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The map label for this gene is bphE [H]

Identifier: 119855032

GI number: 119855032

Start: 152566

End: 153108

Strand: Direct

Name: bphE [H]

Synonym: Mkms_5640

Alternate gene names: 119855032

Gene position: 152566-153108 (Clockwise)

Preceding gene: 119855031

Following gene: 119855033

Centisome position: 50.5

GC content: 59.12

Gene sequence:

>543_bases
ATGACGACAACGGTGCCGCAGGATCCACCTCAGGCTGCGAGGGTTTCGCGCGACGTGCGCGAGGAAATCGAAGACTTCCT
TTTCGACGAGGCCGATCTGTTGGATCGCGATGAGTTCGAAGAGTGGCTGGAATTGCTGGCACCGGATGTGCACTATTTCG
CGCGCGTCCGTCAGACGCTTCGCAGAGCAGGTGGCCCGGGGTTCTCTGAGCGCTCTATGCACCTGAGTGATAACTACACG
AGTCTGAAGATGCGTATTCAGCGGCACCGAACGTCGTCGGATTGGGCTGAGGATCCTCCGTCGCGGATCCGGCACTACAT
CACCAATGTCAGGGTCAGGTCTGGTGAGGACGGCGATGAGTACCGTGTGACGTCGAATGCGTTGCTGGTGCGCACGCGCG
GAGACGAGCCTAAGGTGGAGACAGTCGCTGCCGAGCGCCGAGACATTATTCGTCGCGACGAGACGGGTTTGAAACTGGTC
CAGCGCGAGATCCTGCTGGATCACACCACGTTGCCCACGCACAACCTGTCATTCTGGATCTAA

Upstream 100 bases:

>100_bases
CGGGACGACCGTTGGCCAGTGGCTACGCCGAACAGAATCAGCGCGAGTACTGGAAGCAGTACTTCGACTATCTGGCCACA
CCGAGAAGGGATGAGAACGT

Downstream 100 bases:

>100_bases
GGTGCCGGTGAGCTTGACATCATTACGGCGGCGCCTCCGCCGAAACTCCGGCCCACGAACGTTGTGGCCGAGCGAATTTG
GGTCTGTCCTTAAGGTAGGC

Product: aromatic-ring-hydroxylating dioxygenase, beta subunit

Products: NA

Alternate protein names: Biphenyl 2,3-dioxygenase [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MTTTVPQDPPQAARVSRDVREEIEDFLFDEADLLDRDEFEEWLELLAPDVHYFARVRQTLRRAGGPGFSERSMHLSDNYT
SLKMRIQRHRTSSDWAEDPPSRIRHYITNVRVRSGEDGDEYRVTSNALLVRTRGDEPKVETVAAERRDIIRRDETGLKLV
QREILLDHTTLPTHNLSFWI

Sequences:

>Translated_180_residues
MTTTVPQDPPQAARVSRDVREEIEDFLFDEADLLDRDEFEEWLELLAPDVHYFARVRQTLRRAGGPGFSERSMHLSDNYT
SLKMRIQRHRTSSDWAEDPPSRIRHYITNVRVRSGEDGDEYRVTSNALLVRTRGDEPKVETVAAERRDIIRRDETGLKLV
QREILLDHTTLPTHNLSFWI
>Mature_179_residues
TTTVPQDPPQAARVSRDVREEIEDFLFDEADLLDRDEFEEWLELLAPDVHYFARVRQTLRRAGGPGFSERSMHLSDNYTS
LKMRIQRHRTSSDWAEDPPSRIRHYITNVRVRSGEDGDEYRVTSNALLVRTRGDEPKVETVAAERRDIIRRDETGLKLVQ
REILLDHTTLPTHNLSFWI

Specific function: The beta subunit may be responsible for the substrate specificity of the enzyme [H]

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family [H]

Homologues:

Organism=Escherichia coli, GI1788889, Length=169, Percent_Identity=32.5443786982249, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000391 [H]

Pfam domain/function: PF00866 Ring_hydroxyl_B [H]

EC number: =1.14.12.18 [H]

Molecular weight: Translated: 21087; Mature: 20956

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTVPQDPPQAARVSRDVREEIEDFLFDEADLLDRDEFEEWLELLAPDVHYFARVRQTL
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHH
RRAGGPGFSERSMHLSDNYTSLKMRIQRHRTSSDWAEDPPSRIRHYITNVRVRSGEDGDE
HHCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCEEECCCCCCC
YRVTSNALLVRTRGDEPKVETVAAERRDIIRRDETGLKLVQREILLDHTTLPTHNLSFWI
EEEECCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEECC
>Mature Secondary Structure 
TTTVPQDPPQAARVSRDVREEIEDFLFDEADLLDRDEFEEWLELLAPDVHYFARVRQTL
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHH
RRAGGPGFSERSMHLSDNYTSLKMRIQRHRTSSDWAEDPPSRIRHYITNVRVRSGEDGDE
HHCCCCCCCCCCEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCEEECCCCCCC
YRVTSNALLVRTRGDEPKVETVAAERRDIIRRDETGLKLVQREILLDHTTLPTHNLSFWI
EEEECCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8890734 [H]