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The map label for this gene is hcaE [H]

Identifier: 119855018

GI number: 119855018

Start: 138142

End: 139518

Strand: Direct

Name: hcaE [H]

Synonym: Mkms_5626

Alternate gene names: 119855018

Gene position: 138142-139518 (Clockwise)

Preceding gene: 119855017

Following gene: 119855019

Centisome position: 45.73

GC content: 63.33

Gene sequence:

>1377_bases
ATGACCACCGAAACAACCGAAACCACCGGCACAGCTGACGCGACCGATCCCTACCTGCGGCGCGCGTTGCGGGAGGTAGC
GGACGGGCTCAAGGTCGGGCGCTTACCGGCCCGCGTCGTCAGCGATCCCGCGCTACACACGATCGAGATGGAGCGGATCT
TCGGGCGCGCCTGGGTGTTTCTCGGACACGAGTCGGAGTTGGCCAAGTCCGGCGACTTCGTCGTGCGGCACATCGGGGCC
GATTCGGTGATCGTTTGCCGGGACAACTCCGGCCGCATCCAGGCGCTGTCCAATTCTTGTCGCCACCGTGGTGCGCTCGT
GTGCCGCGCTGAGATGGGAAACACCGCGCACTTCCAATGCCCGTACCACGGCTGGGTGTACAGCAACACCGGAGAGCTCG
TCGGCGTGCCGGCGATGAGTGAGGCCTATCCCGGCGGCTTCGACAAGTCGCAGTGGGGATTACGTCACATCCCCCATGTC
GACTCGTACGCCGGATTCATCTTCGGCAGCGTCGATCCGAAGGCGCCGAGCCTGACCGACTACCTCGGCGACACGACGTT
CTACCTCGACCTCATTGCGAAGAAGACAGCGGGCGGTCTGGAGGTGATAGGGGCACCGCATCGATGGGTGATGTCAGCGA
ACTGGAAGACAGCCGCCGACAATTTTGTCGGCGACTCCTACCACACCCTCTTTGCTCACCGCTCGATGGTCGAGCTAGGC
ATGGCGCCCGGTGACCCAAACTTCGCGAGCGCACCAGCGGAGATCTCGCTGCAGAACGGCCACGGCGTCGGCGTACTCGG
CTTTCCGCCCACGCTCGCCGATTTTCCCGAGTACGAGGGATACCCCGACGAAGTCGTCGACCAGATGGCGACGTCCTACC
CGTCGCCGGTACACAAGGACCTGATGCGACGCTCATCCTTTATTCACGGCACCGTGTTCCCGAATTTGTCGTTCATCAAC
GTGACCCTCGCGCCGGACCACATGTCGCCCCCTACCCCCTTCATCACGTTCCGGGTATGGCATCCGCTCTCCCATGATCG
GATGGAGATCCTCTCCTGGTTCCTGGTCGAACGCGATGCTCCGGAATGGTTGCGCGATGCGTCCCAGGCGTCCTACGTCA
ACAACTTCGGCCCAGGTGGGGTTTTCGAACAGGACGACGCCGAGGCATGGAAGGCCATCACCGAATCTGTCCAGGGCCCG
TTCGCCGGTGAAGGCCTGCTGAACTACGAAATGGGCATGGACTTGACTCCGCTCACCGACTGGCCAGGGCCGGGAGAGGC
CCTCCCGAGCGGGTACGCCGAGCAGAATCAGCGGCGGTTTTGGGGGAGATGGCTGGAATACATGGGTCAGCCTCCCGCAT
TCGGCGGGCGTGCTTGA

Upstream 100 bases:

>100_bases
CGACGTTGCCCATCGACAACCTGTCGGTATTCCTGTAGGGCCAGCTCCCCTCACCGCCTTCCTCCTTGGCTATCGCCTAC
TCCAGAAAGGGTCCAACCAT

Downstream 100 bases:

>100_bases
TGAAGCTTCTGCCCAGGCTATGCGCGAAGTGCGCCCGTTCACCAATCGGCGCCGCGTGAGTGCCGTGAAGGCGGGGGACC
GCCGGGTCGCAATTGTCACT

Product: ring hydroxylating dioxygenase, alpha subunit

Products: NA

Alternate protein names: Digoxigenin subunit alpha [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTTETTETTGTADATDPYLRRALREVADGLKVGRLPARVVSDPALHTIEMERIFGRAWVFLGHESELAKSGDFVVRHIGA
DSVIVCRDNSGRIQALSNSCRHRGALVCRAEMGNTAHFQCPYHGWVYSNTGELVGVPAMSEAYPGGFDKSQWGLRHIPHV
DSYAGFIFGSVDPKAPSLTDYLGDTTFYLDLIAKKTAGGLEVIGAPHRWVMSANWKTAADNFVGDSYHTLFAHRSMVELG
MAPGDPNFASAPAEISLQNGHGVGVLGFPPTLADFPEYEGYPDEVVDQMATSYPSPVHKDLMRRSSFIHGTVFPNLSFIN
VTLAPDHMSPPTPFITFRVWHPLSHDRMEILSWFLVERDAPEWLRDASQASYVNNFGPGGVFEQDDAEAWKAITESVQGP
FAGEGLLNYEMGMDLTPLTDWPGPGEALPSGYAEQNQRRFWGRWLEYMGQPPAFGGRA

Sequences:

>Translated_458_residues
MTTETTETTGTADATDPYLRRALREVADGLKVGRLPARVVSDPALHTIEMERIFGRAWVFLGHESELAKSGDFVVRHIGA
DSVIVCRDNSGRIQALSNSCRHRGALVCRAEMGNTAHFQCPYHGWVYSNTGELVGVPAMSEAYPGGFDKSQWGLRHIPHV
DSYAGFIFGSVDPKAPSLTDYLGDTTFYLDLIAKKTAGGLEVIGAPHRWVMSANWKTAADNFVGDSYHTLFAHRSMVELG
MAPGDPNFASAPAEISLQNGHGVGVLGFPPTLADFPEYEGYPDEVVDQMATSYPSPVHKDLMRRSSFIHGTVFPNLSFIN
VTLAPDHMSPPTPFITFRVWHPLSHDRMEILSWFLVERDAPEWLRDASQASYVNNFGPGGVFEQDDAEAWKAITESVQGP
FAGEGLLNYEMGMDLTPLTDWPGPGEALPSGYAEQNQRRFWGRWLEYMGQPPAFGGRA
>Mature_457_residues
TTETTETTGTADATDPYLRRALREVADGLKVGRLPARVVSDPALHTIEMERIFGRAWVFLGHESELAKSGDFVVRHIGAD
SVIVCRDNSGRIQALSNSCRHRGALVCRAEMGNTAHFQCPYHGWVYSNTGELVGVPAMSEAYPGGFDKSQWGLRHIPHVD
SYAGFIFGSVDPKAPSLTDYLGDTTFYLDLIAKKTAGGLEVIGAPHRWVMSANWKTAADNFVGDSYHTLFAHRSMVELGM
APGDPNFASAPAEISLQNGHGVGVLGFPPTLADFPEYEGYPDEVVDQMATSYPSPVHKDLMRRSSFIHGTVFPNLSFINV
TLAPDHMSPPTPFITFRVWHPLSHDRMEILSWFLVERDAPEWLRDASQASYVNNFGPGGVFEQDDAEAWKAITESVQGPF
AGEGLLNYEMGMDLTPLTDWPGPGEALPSGYAEQNQRRFWGRWLEYMGQPPAFGGRA

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=437, Percent_Identity=36.1556064073227, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1788103, Length=204, Percent_Identity=31.3725490196078, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 50457; Mature: 50326

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTETTETTGTADATDPYLRRALREVADGLKVGRLPARVVSDPALHTIEMERIFGRAWVF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHCCCCCHHHHCCCCHHHHHHHHHHCEEEEE
LGHESELAKSGDFVVRHIGADSVIVCRDNSGRIQALSNSCRHRGALVCRAEMGNTAHFQC
ECCHHHHHCCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCCEEEEC
PYHGWVYSNTGELVGVPAMSEAYPGGFDKSQWGLRHIPHVDSYAGFIFGSVDPKAPSLTD
CCCCEEECCCCCEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHH
YLGDTTFYLDLIAKKTAGGLEVIGAPHRWVMSANWKTAADNFVGDSYHTLFAHRSMVELG
HHCCCEEEEHHHHHHCCCCEEEECCCCEEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHC
MAPGDPNFASAPAEISLQNGHGVGVLGFPPTLADFPEYEGYPDEVVDQMATSYPSPVHKD
CCCCCCCCCCCCCEEEEECCCEEEEECCCCCHHCCCCCCCCHHHHHHHHHHCCCCHHHHH
LMRRSSFIHGTVFPNLSFINVTLAPDHMSPPTPFITFRVWHPLSHDRMEILSWFLVERDA
HHHHHCCEEEEECCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCC
PEWLRDASQASYVNNFGPGGVFEQDDAEAWKAITESVQGPFAGEGLLNYEMGMDLTPLTD
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCC
WPGPGEALPSGYAEQNQRRFWGRWLEYMGQPPAFGGRA
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TTETTETTGTADATDPYLRRALREVADGLKVGRLPARVVSDPALHTIEMERIFGRAWVF
CCCCCCCCCCCCCCCHHHHHHHHHHHCCHHCCCCCHHHHCCCCHHHHHHHHHHCEEEEE
LGHESELAKSGDFVVRHIGADSVIVCRDNSGRIQALSNSCRHRGALVCRAEMGNTAHFQC
ECCHHHHHCCCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHCCEEEEEECCCCCEEEEC
PYHGWVYSNTGELVGVPAMSEAYPGGFDKSQWGLRHIPHVDSYAGFIFGSVDPKAPSLTD
CCCCEEECCCCCEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHH
YLGDTTFYLDLIAKKTAGGLEVIGAPHRWVMSANWKTAADNFVGDSYHTLFAHRSMVELG
HHCCCEEEEHHHHHHCCCCEEEECCCCEEEEECCCCCCHHHCCCCCHHHHHHHHHHHHHC
MAPGDPNFASAPAEISLQNGHGVGVLGFPPTLADFPEYEGYPDEVVDQMATSYPSPVHKD
CCCCCCCCCCCCCEEEEECCCEEEEECCCCCHHCCCCCCCCHHHHHHHHHHCCCCHHHHH
LMRRSSFIHGTVFPNLSFINVTLAPDHMSPPTPFITFRVWHPLSHDRMEILSWFLVERDA
HHHHHCCEEEEECCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHCCCC
PEWLRDASQASYVNNFGPGGVFEQDDAEAWKAITESVQGPFAGEGLLNYEMGMDLTPLTD
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCC
WPGPGEALPSGYAEQNQRRFWGRWLEYMGQPPAFGGRA
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]