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The map label for this gene is bphA [H]

Identifier: 119855003

GI number: 119855003

Start: 120610

End: 121926

Strand: Direct

Name: bphA [H]

Synonym: Mkms_5610

Alternate gene names: 119855003

Gene position: 120610-121926 (Clockwise)

Preceding gene: 119855002

Following gene: 119855004

Centisome position: 39.93

GC content: 59.68

Gene sequence:

>1317_bases
ATGGAGACAATCGGTTCCAAAACCGGCTCGGCGCTGCCGATAGACTCCTCGGCCTTCTACGACGAGGCGGTGTACCAGCG
GGAACTCGACTCTATCTTCAAGCGGTCGTGGCTATTCGTCGGCCACGAGTCCATGATCCCCAAGCCCGGTGACTTCCGCA
CAACCTATATGGCCGACGACGCGGTGATCGTGTGCCGGGACAAGGAATCTCGTGTTCGTGTGCTGCTGAACAAATGTCGG
CACCGGGGCAATAAAGTCTGTCAGTTCGACAAGGGCAACGCGAACATCTTTCACTGCAGCTATCACGGCTGGAGTTACGA
CACTGCCGGACGGCTGCGCAGTGTTCCGCTAGCCGAGAGCGCCTACGGGCCGCAGTTCGATAAGGCCAGCATGGGCCTCG
TCTCCCCCCGGGTAGCTACCTACAAAGGACTTATCTTTGCGTGCTGGGATCAGTCGGCGCCGCCGCTGGAAGAATATCTG
GGGGAGGGCCTGCTGTGGTACCTCGACAACTTTCTACTCGACTGTGACCCCAACGGCCTGCAGGTCGTTCCCGGCCTGCA
CCGCTACCTCATGCCCGTCAACTGGAAGTTGTTGGCCGAGAACTTCGGCGGCGACCAGTATCACTTCGCTGCCACTCACG
GATCGGTTTCCGCACTGTCGAAAGCTGGGCAGACTGCCCGCATCAACTTTTCGATAGACGAGGGGCAGCACTACAGCGTG
GTTCTCGACGGCTGCGCGCCCCATGGGTTGCTGCAACTGGCAGTCGGTAAGAATTTTTATCAGGACGATCTCGCCCAAGC
CGAAACGCTGGGTACCGAAGCAGTCGACTGGCTAACCGAGCGGCAGCGCCTGCAAGACGAGCGGTTGGCGACATCCCCCG
TGCAGCCCTACAGCTTTCACGTAGCCAATATCTTCCCAAACTTCAGCATGATTGGCATGGGCACGGCTTTTTACGGTCGG
GGATTCATCATGTGGCAGCCTCGGGGACCGCGGTTGACCGAGGTTTGGGAGTGGTGTTTGGTGGAAAGCTCCGCGCCCCG
TGCTGTCAAGGAACGCATGGTCTTCGTCTTGAGTCAGCGGCAATCGGCGGCCGGTCTCGTGACGCCCGATGATCACGAAA
ACTTCGAACGATTGTCTGACGCCCTTGACACCGGAGTCGCCCGGGATGTGCCGTTCAACTACTCACTCGGCGAGGACGTC
GAACCAATGGAGTCGCTGGTTGCGGAGTTACCCGGCAATGTCAGGCCGCAGATCAGCGAAGCCTACCAGCGCGAGTTCTA
TCGGCACTGGCACCGAACTATGACGGAGCCGGCCTAG

Upstream 100 bases:

>100_bases
AGGATTGTGGCCATTGGCCGCAGTGGGAACGGCCGGAAGAGTTCAACCAAATCCTCACCAATTATCTTCAAGGTGATCGC
GAGTCCCGAGGGCAACGTTG

Downstream 100 bases:

>100_bases
TCATGGAGACCAGCCAGTTGGACGTGGAACTGCGGCTGCGAATAAACGAATTCTATGCGCGGGAGACCATGCTTCTCGAT
GATGGGCGGCTGGACGAGTG

Product: Rieske (2Fe-2S) domain-containing protein

Products: NA

Alternate protein names: Biphenyl 2,3-dioxygenase [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
METIGSKTGSALPIDSSAFYDEAVYQRELDSIFKRSWLFVGHESMIPKPGDFRTTYMADDAVIVCRDKESRVRVLLNKCR
HRGNKVCQFDKGNANIFHCSYHGWSYDTAGRLRSVPLAESAYGPQFDKASMGLVSPRVATYKGLIFACWDQSAPPLEEYL
GEGLLWYLDNFLLDCDPNGLQVVPGLHRYLMPVNWKLLAENFGGDQYHFAATHGSVSALSKAGQTARINFSIDEGQHYSV
VLDGCAPHGLLQLAVGKNFYQDDLAQAETLGTEAVDWLTERQRLQDERLATSPVQPYSFHVANIFPNFSMIGMGTAFYGR
GFIMWQPRGPRLTEVWEWCLVESSAPRAVKERMVFVLSQRQSAAGLVTPDDHENFERLSDALDTGVARDVPFNYSLGEDV
EPMESLVAELPGNVRPQISEAYQREFYRHWHRTMTEPA

Sequences:

>Translated_438_residues
METIGSKTGSALPIDSSAFYDEAVYQRELDSIFKRSWLFVGHESMIPKPGDFRTTYMADDAVIVCRDKESRVRVLLNKCR
HRGNKVCQFDKGNANIFHCSYHGWSYDTAGRLRSVPLAESAYGPQFDKASMGLVSPRVATYKGLIFACWDQSAPPLEEYL
GEGLLWYLDNFLLDCDPNGLQVVPGLHRYLMPVNWKLLAENFGGDQYHFAATHGSVSALSKAGQTARINFSIDEGQHYSV
VLDGCAPHGLLQLAVGKNFYQDDLAQAETLGTEAVDWLTERQRLQDERLATSPVQPYSFHVANIFPNFSMIGMGTAFYGR
GFIMWQPRGPRLTEVWEWCLVESSAPRAVKERMVFVLSQRQSAAGLVTPDDHENFERLSDALDTGVARDVPFNYSLGEDV
EPMESLVAELPGNVRPQISEAYQREFYRHWHRTMTEPA
>Mature_438_residues
METIGSKTGSALPIDSSAFYDEAVYQRELDSIFKRSWLFVGHESMIPKPGDFRTTYMADDAVIVCRDKESRVRVLLNKCR
HRGNKVCQFDKGNANIFHCSYHGWSYDTAGRLRSVPLAESAYGPQFDKASMGLVSPRVATYKGLIFACWDQSAPPLEEYL
GEGLLWYLDNFLLDCDPNGLQVVPGLHRYLMPVNWKLLAENFGGDQYHFAATHGSVSALSKAGQTARINFSIDEGQHYSV
VLDGCAPHGLLQLAVGKNFYQDDLAQAETLGTEAVDWLTERQRLQDERLATSPVQPYSFHVANIFPNFSMIGMGTAFYGR
GFIMWQPRGPRLTEVWEWCLVESSAPRAVKERMVFVLSQRQSAAGLVTPDDHENFERLSDALDTGVARDVPFNYSLGEDV
EPMESLVAELPGNVRPQISEAYQREFYRHWHRTMTEPA

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=433, Percent_Identity=35.3348729792148, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1788103, Length=224, Percent_Identity=24.1071428571429, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.18 [H]

Molecular weight: Translated: 49377; Mature: 49377

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIGSKTGSALPIDSSAFYDEAVYQRELDSIFKRSWLFVGHESMIPKPGDFRTTYMADD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCEEEEEECC
AVIVCRDKESRVRVLLNKCRHRGNKVCQFDKGNANIFHCSYHGWSYDTAGRLRSVPLAES
EEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCC
AYGPQFDKASMGLVSPRVATYKGLIFACWDQSAPPLEEYLGEGLLWYLDNFLLDCDPNGL
CCCCCCCCCCCCCCCCCHHHHCCEEEEECCCCCCCHHHHHCCCHHHHHHHHEEECCCCCC
QVVPGLHRYLMPVNWKLLAENFGGDQYHFAATHGSVSALSKAGQTARINFSIDEGQHYSV
EECCCHHHHHCCCCEEEEEECCCCCEEEEEECCCCHHHHHHCCCEEEEEEEECCCCEEEE
VLDGCAPHGLLQLAVGKNFYQDDLAQAETLGTEAVDWLTERQRLQDERLATSPVQPYSFH
EEECCCCCCCEEHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
VANIFPNFSMIGMGTAFYGRGFIMWQPRGPRLTEVWEWCLVESSAPRAVKERMVFVLSQR
EEECCCCCEEEECCHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
QSAAGLVTPDDHENFERLSDALDTGVARDVPFNYSLGEDVEPMESLVAELPGNVRPQISE
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHH
AYQREFYRHWHRTMTEPA
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
METIGSKTGSALPIDSSAFYDEAVYQRELDSIFKRSWLFVGHESMIPKPGDFRTTYMADD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCEEEEEECC
AVIVCRDKESRVRVLLNKCRHRGNKVCQFDKGNANIFHCSYHGWSYDTAGRLRSVPLAES
EEEEEECCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCC
AYGPQFDKASMGLVSPRVATYKGLIFACWDQSAPPLEEYLGEGLLWYLDNFLLDCDPNGL
CCCCCCCCCCCCCCCCCHHHHCCEEEEECCCCCCCHHHHHCCCHHHHHHHHEEECCCCCC
QVVPGLHRYLMPVNWKLLAENFGGDQYHFAATHGSVSALSKAGQTARINFSIDEGQHYSV
EECCCHHHHHCCCCEEEEEECCCCCEEEEEECCCCHHHHHHCCCEEEEEEEECCCCEEEE
VLDGCAPHGLLQLAVGKNFYQDDLAQAETLGTEAVDWLTERQRLQDERLATSPVQPYSFH
EEECCCCCCCEEHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
VANIFPNFSMIGMGTAFYGRGFIMWQPRGPRLTEVWEWCLVESSAPRAVKERMVFVLSQR
EEECCCCCEEEECCHHHHCCEEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
QSAAGLVTPDDHENFERLSDALDTGVARDVPFNYSLGEDVEPMESLVAELPGNVRPQISE
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHH
AYQREFYRHWHRTMTEPA
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1569021; 7592331 [H]