Click here to switch to the map view.

The map label for this gene is hcaF [H]

Identifier: 119855004

GI number: 119855004

Start: 121929

End: 122441

Strand: Direct

Name: hcaF [H]

Synonym: Mkms_5611

Alternate gene names: 119855004

Gene position: 121929-122441 (Clockwise)

Preceding gene: 119855003

Following gene: 119855005

Centisome position: 40.36

GC content: 57.89

Gene sequence:

>513_bases
ATGGAGACCAGCCAGTTGGACGTGGAACTGCGGCTGCGAATAAACGAATTCTATGCGCGGGAGACCATGCTTCTCGATGA
TGGGCGGCTGGACGAGTGGCTGGGCTTATTGGCGCCGGATGTCAGATACACGATGCCATTCCCAGAGTCCAGCACGCATC
CGGTCGACGATGATGACGGCCTACCGCCGTTTCTGTTGTTCAACGACGACTACGAATCGCTGAAGCTCAGAATTGCCCGG
CTGGCTACTGGGCTCGCCCCCGGGGAAACTCCACGCACGATCACTCAGCGCTTAGTCAGCGACATCCTGGTCACCGATGT
ATCCGATCAGGAGCTGAGCGTCCGATCCAGCGTCATGGTGTTTCTCGTACGCCATGAACGCCACGAGAGCTTCTTCATCG
GCAAACGACAAGACGTTCTACGCCGAGACGAAGACTCGGGCCTGCTGCTGGCCGCCCGGGTCATCACCCTGGCTCATAGC
GTTCTTCCACGAGCGATTTCGATCTTCTTCTAA

Upstream 100 bases:

>100_bases
TTGCGGAGTTACCCGGCAATGTCAGGCCGCAGATCAGCGAAGCCTACCAGCGCGAGTTCTATCGGCACTGGCACCGAACT
ATGACGGAGCCGGCCTAGTC

Downstream 100 bases:

>100_bases
TGCCCAGTCGTTCACTAGCTCTCAGTTAGGTCGCACATGACAGATGTCGCGTCACGCGAGCTCGGCCTAGGATTTCTCGG
AATCGGTCAGGCTGTCGCCC

Product: aromatic-ring-hydroxylating dioxygenase, beta subunit

Products: NA

Alternate protein names: Digoxigenin subunit beta [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
METSQLDVELRLRINEFYARETMLLDDGRLDEWLGLLAPDVRYTMPFPESSTHPVDDDDGLPPFLLFNDDYESLKLRIAR
LATGLAPGETPRTITQRLVSDILVTDVSDQELSVRSSVMVFLVRHERHESFFIGKRQDVLRRDEDSGLLLAARVITLAHS
VLPRAISIFF

Sequences:

>Translated_170_residues
METSQLDVELRLRINEFYARETMLLDDGRLDEWLGLLAPDVRYTMPFPESSTHPVDDDDGLPPFLLFNDDYESLKLRIAR
LATGLAPGETPRTITQRLVSDILVTDVSDQELSVRSSVMVFLVRHERHESFFIGKRQDVLRRDEDSGLLLAARVITLAHS
VLPRAISIFF
>Mature_170_residues
METSQLDVELRLRINEFYARETMLLDDGRLDEWLGLLAPDVRYTMPFPESSTHPVDDDDGLPPFLLFNDDYESLKLRIAR
LATGLAPGETPRTITQRLVSDILVTDVSDQELSVRSSVMVFLVRHERHESFFIGKRQDVLRRDEDSGLLLAARVITLAHS
VLPRAISIFF

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family [H]

Homologues:

Organism=Escherichia coli, GI1788889, Length=173, Percent_Identity=34.6820809248555, Blast_Score=93, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000391 [H]

Pfam domain/function: PF00866 Ring_hydroxyl_B [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 19442; Mature: 19442

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METSQLDVELRLRINEFYARETMLLDDGRLDEWLGLLAPDVRYTMPFPESSTHPVDDDDG
CCCCCCCEEEEEEEHHHHHHHEEEECCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCC
LPPFLLFNDDYESLKLRIARLATGLAPGETPRTITQRLVSDILVTDVSDQELSVRSSVMV
CCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
FLVRHERHESFFIGKRQDVLRRDEDSGLLLAARVITLAHSVLPRAISIFF
HEEHHCCCCCEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
METSQLDVELRLRINEFYARETMLLDDGRLDEWLGLLAPDVRYTMPFPESSTHPVDDDDG
CCCCCCCEEEEEEEHHHHHHHEEEECCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCC
LPPFLLFNDDYESLKLRIARLATGLAPGETPRTITQRLVSDILVTDVSDQELSVRSSVMV
CCCEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
FLVRHERHESFFIGKRQDVLRRDEDSGLLLAARVITLAHSVLPRAISIFF
HEEHHCCCCCEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA