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The map label for this gene is hupL [H]

Identifier: 119900076

GI number: 119900076

Start: 4148006

End: 4149802

Strand: Direct

Name: hupL [H]

Synonym: azo3787

Alternate gene names: 119900076

Gene position: 4148006-4149802 (Clockwise)

Preceding gene: 119900075

Following gene: 119900077

Centisome position: 94.79

GC content: 64.83

Gene sequence:

>1797_bases
ATGAACATGGGCGTGTACGAAACTCAGGGCTTCAAGCTCGACAACACCGGCAAGCGCGTCGTCGTCGATCCGGTCACCCG
CATCGAAGGCCACATGCGCGTCGAGGTGAACCTCGACAGCAACAACGTGATCCGCAACGCGGTGTCCACCGGCACCATGT
GGCGCGGCCTCGAAGTCATCCTCAAGGGCCGCGACCCGCGCGACGCCTGGGCCTTCACCGAACGCATCTGCGGCGTGTGC
ACCGGCACCCATGCGCTGACCAGCGTGCGCGCGGTGGAAGACGCGCTCGGCATCGACATCCCGGAGAACGCCAACTCCAT
CCGCAACCTGATGCAGCTCACGCTGCAGGTGCATGACCACCTGGTGCACTTCTACCACCTGCACGCGCTCGACTGGGTGG
ACGTGGTCAGCGCGCTGAAGGCCGACCCCAAGAAGACCTCCGAACTCGCGCAGAGCATCTCGAGCTGGCCGAACTCTTCG
CCAGGTTACTTCCGCGACATCCAGAACCGGCTGAAGAAGTTCGTCGAATCCGGCCAGCTCGGCCCCTTCATGAACGGCTA
CTGGGGCAACCCCGCCTACAAGCTGCCGCCCGAAGCCAACCTGATGGCGGTGACGCACTACCTGGAAGCGCTCGACTTCC
AGAAGGACATCGTCAAGATCCACACCATCTTCGGCGGCAAGAACCCGCACCCGAACTGGCTGGTGGGCGGCATGCCGTGC
GCGATCAACCTCGACGGCGTGGGCGCCGTAGGCGCGGTCAACATGGAACGGCTGAACCTGGTCAAGAGCATCATCGACCG
CTCGATCGAGTTCATCGACCAGGTCTATATCCCCGACCTCGTCGCCATCGCCAGCTTCTACAAGGACTGGACCTACGGCG
GCGGTATCTCCTCGCTGTCGCTGCTGTCCTACGGTGACATCCCGGACCGCGCCAACGACTACGGCGCCGGCAACCTGCTG
CTGCCGCGCGGCGCCATCATCAACGGCGACCTCAGCAAGATCCACGAGGTGGACCTGCGCGATCCCGAGGAGATCCAGGA
GTTCGTCTCCCACTCCTGGTACAAGTACGCCGACGAAACCAAGGGTCTGCACCCCTGGGACGGCGTCACCGAACCCAACT
TCGTGCTCGGCAAGAACACCAAGGGCACCAAGACCAACGTCCAGTCCTTCGACGAGGAAGGCAAGTACTCCTGGATCAAG
TCGCCGCGCTGGCGCGGCCACGCGATGGAAGTCGGCCCGCTGGCGCGGTACGTGATCGGCTACGTGCAGGGCAATCCGGA
ATTCAAGGAGCCGGTCGACAAGCTGCTGAAGGACCTCGGCCTGCCGCTGCAGGCGATCTTCTCGACGCTGGGCCGCACCG
CGGCGCGCGGCCTGGAGGCCTCGTGGGCGGCGCACAAGATGGCCTATTTCTACGACAAGCTGATCGCCAACATCAAGGCC
GGCGACACCGCCACCGCCAACGTCGAGAAGTGGGATCCGAGCACCTGGCCGAAGGAGGTCAAGGGCGTGGGCTTCACCGA
GGCGCCGCGCGGCGCGCTGGGCCACTGGATCAAGATCAAGGACGGCAAGATCGACAACTACCAGGCCGTGGTGCCCACCA
CCTGGAACGGCAGCCCGCGCGACCACAAGGGCCAGATCGGCGCCTTCGAGGCCAGCCTGATGAACACCCCGGTGGCCAAG
GCCGACGAACCGCTGGAGATCCTGCGCACGCTGCACTCGTTCGACCCGTGCCTGGCCTGCTCCACGCACGTGATGAGCCC
CGACGGACAGGAGATGGCCCAGGTGAAAGTGCGCTGA

Upstream 100 bases:

>100_bases
CCGCCGGCGTGGTCGGCGCGAGCGTGGCCGCGCATGCCGCGGTCACCGCGCTGGCACGCGCGCGCAGCAAGACCGGCATC
GACGTGAAGCAAGGGGAAGA

Downstream 100 bases:

>100_bases
TTGACGGGGCGAGGACCGGTCGGGCGCACCCCTCACGACAGGCGGAGCACCCTCCCTCCCCAAGCCTCATGGGCCGCCTG
CGGCCCCCGGCCCCCTCGCC

Product: ferredoxin hydrogenase large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MNMGVYETQGFKLDNTGKRVVVDPVTRIEGHMRVEVNLDSNNVIRNAVSTGTMWRGLEVILKGRDPRDAWAFTERICGVC
TGTHALTSVRAVEDALGIDIPENANSIRNLMQLTLQVHDHLVHFYHLHALDWVDVVSALKADPKKTSELAQSISSWPNSS
PGYFRDIQNRLKKFVESGQLGPFMNGYWGNPAYKLPPEANLMAVTHYLEALDFQKDIVKIHTIFGGKNPHPNWLVGGMPC
AINLDGVGAVGAVNMERLNLVKSIIDRSIEFIDQVYIPDLVAIASFYKDWTYGGGISSLSLLSYGDIPDRANDYGAGNLL
LPRGAIINGDLSKIHEVDLRDPEEIQEFVSHSWYKYADETKGLHPWDGVTEPNFVLGKNTKGTKTNVQSFDEEGKYSWIK
SPRWRGHAMEVGPLARYVIGYVQGNPEFKEPVDKLLKDLGLPLQAIFSTLGRTAARGLEASWAAHKMAYFYDKLIANIKA
GDTATANVEKWDPSTWPKEVKGVGFTEAPRGALGHWIKIKDGKIDNYQAVVPTTWNGSPRDHKGQIGAFEASLMNTPVAK
ADEPLEILRTLHSFDPCLACSTHVMSPDGQEMAQVKVR

Sequences:

>Translated_598_residues
MNMGVYETQGFKLDNTGKRVVVDPVTRIEGHMRVEVNLDSNNVIRNAVSTGTMWRGLEVILKGRDPRDAWAFTERICGVC
TGTHALTSVRAVEDALGIDIPENANSIRNLMQLTLQVHDHLVHFYHLHALDWVDVVSALKADPKKTSELAQSISSWPNSS
PGYFRDIQNRLKKFVESGQLGPFMNGYWGNPAYKLPPEANLMAVTHYLEALDFQKDIVKIHTIFGGKNPHPNWLVGGMPC
AINLDGVGAVGAVNMERLNLVKSIIDRSIEFIDQVYIPDLVAIASFYKDWTYGGGISSLSLLSYGDIPDRANDYGAGNLL
LPRGAIINGDLSKIHEVDLRDPEEIQEFVSHSWYKYADETKGLHPWDGVTEPNFVLGKNTKGTKTNVQSFDEEGKYSWIK
SPRWRGHAMEVGPLARYVIGYVQGNPEFKEPVDKLLKDLGLPLQAIFSTLGRTAARGLEASWAAHKMAYFYDKLIANIKA
GDTATANVEKWDPSTWPKEVKGVGFTEAPRGALGHWIKIKDGKIDNYQAVVPTTWNGSPRDHKGQIGAFEASLMNTPVAK
ADEPLEILRTLHSFDPCLACSTHVMSPDGQEMAQVKVR
>Mature_598_residues
MNMGVYETQGFKLDNTGKRVVVDPVTRIEGHMRVEVNLDSNNVIRNAVSTGTMWRGLEVILKGRDPRDAWAFTERICGVC
TGTHALTSVRAVEDALGIDIPENANSIRNLMQLTLQVHDHLVHFYHLHALDWVDVVSALKADPKKTSELAQSISSWPNSS
PGYFRDIQNRLKKFVESGQLGPFMNGYWGNPAYKLPPEANLMAVTHYLEALDFQKDIVKIHTIFGGKNPHPNWLVGGMPC
AINLDGVGAVGAVNMERLNLVKSIIDRSIEFIDQVYIPDLVAIASFYKDWTYGGGISSLSLLSYGDIPDRANDYGAGNLL
LPRGAIINGDLSKIHEVDLRDPEEIQEFVSHSWYKYADETKGLHPWDGVTEPNFVLGKNTKGTKTNVQSFDEEGKYSWIK
SPRWRGHAMEVGPLARYVIGYVQGNPEFKEPVDKLLKDLGLPLQAIFSTLGRTAARGLEASWAAHKMAYFYDKLIANIKA
GDTATANVEKWDPSTWPKEVKGVGFTEAPRGALGHWIKIKDGKIDNYQAVVPTTWNGSPRDHKGQIGAFEASLMNTPVAK
ADEPLEILRTLHSFDPCLACSTHVMSPDGQEMAQVKVR

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=594, Percent_Identity=66.3299663299663, Blast_Score=850, Evalue=0.0,
Organism=Escherichia coli, GI1789368, Length=592, Percent_Identity=46.6216216216216, Blast_Score=520, Evalue=1e-148,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 66390; Mature: 66390

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMGVYETQGFKLDNTGKRVVVDPVTRIEGHMRVEVNLDSNNVIRNAVSTGTMWRGLEVI
CCCCCEECCCEEECCCCCEEEECCHHHCCCEEEEEEEECCCHHHHHHHHHCHHHHCHHHE
LKGRDPRDAWAFTERICGVCTGTHALTSVRAVEDALGIDIPENANSIRNLMQLTLQVHDH
EECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LVHFYHLHALDWVDVVSALKADPKKTSELAQSISSWPNSSPGYFRDIQNRLKKFVESGQL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
GPFMNGYWGNPAYKLPPEANLMAVTHYLEALDFQKDIVKIHTIFGGKNPHPNWLVGGMPC
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEHHEECCCCCCCCEEECCCCE
AINLDGVGAVGAVNMERLNLVKSIIDRSIEFIDQVYIPDLVAIASFYKDWTYGGGISSLS
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LLSYGDIPDRANDYGAGNLLLPRGAIINGDLSKIHEVDLRDPEEIQEFVSHSWYKYADET
HHCCCCCCCCCCCCCCCCEEECCCCEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
KGLHPWDGVTEPNFVLGKNTKGTKTNVQSFDEEGKYSWIKSPRWRGHAMEVGPLARYVIG
CCCCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHH
YVQGNPEFKEPVDKLLKDLGLPLQAIFSTLGRTAARGLEASWAAHKMAYFYDKLIANIKA
HCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
GDTATANVEKWDPSTWPKEVKGVGFTEAPRGALGHWIKIKDGKIDNYQAVVPTTWNGSPR
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCC
DHKGQIGAFEASLMNTPVAKADEPLEILRTLHSFDPCLACSTHVMSPDGQEMAQVKVR
CCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHEECC
>Mature Secondary Structure
MNMGVYETQGFKLDNTGKRVVVDPVTRIEGHMRVEVNLDSNNVIRNAVSTGTMWRGLEVI
CCCCCEECCCEEECCCCCEEEECCHHHCCCEEEEEEEECCCHHHHHHHHHCHHHHCHHHE
LKGRDPRDAWAFTERICGVCTGTHALTSVRAVEDALGIDIPENANSIRNLMQLTLQVHDH
EECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LVHFYHLHALDWVDVVSALKADPKKTSELAQSISSWPNSSPGYFRDIQNRLKKFVESGQL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
GPFMNGYWGNPAYKLPPEANLMAVTHYLEALDFQKDIVKIHTIFGGKNPHPNWLVGGMPC
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEHHEECCCCCCCCEEECCCCE
AINLDGVGAVGAVNMERLNLVKSIIDRSIEFIDQVYIPDLVAIASFYKDWTYGGGISSLS
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LLSYGDIPDRANDYGAGNLLLPRGAIINGDLSKIHEVDLRDPEEIQEFVSHSWYKYADET
HHCCCCCCCCCCCCCCCCEEECCCCEECCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
KGLHPWDGVTEPNFVLGKNTKGTKTNVQSFDEEGKYSWIKSPRWRGHAMEVGPLARYVIG
CCCCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHH
YVQGNPEFKEPVDKLLKDLGLPLQAIFSTLGRTAARGLEASWAAHKMAYFYDKLIANIKA
HCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
GDTATANVEKWDPSTWPKEVKGVGFTEAPRGALGHWIKIKDGKIDNYQAVVPTTWNGSPR
CCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCC
DHKGQIGAFEASLMNTPVAKADEPLEILRTLHSFDPCLACSTHVMSPDGQEMAQVKVR
CCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCCCHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3054886; 12597275; 8163174 [H]