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The map label for this gene is hupS [H]

Identifier: 119900075

GI number: 119900075

Start: 4146921

End: 4148009

Strand: Direct

Name: hupS [H]

Synonym: azo3786

Alternate gene names: 119900075

Gene position: 4146921-4148009 (Clockwise)

Preceding gene: 119900072

Following gene: 119900076

Centisome position: 94.76

GC content: 66.76

Gene sequence:

>1089_bases
ATGACCGAAACCTTTTACGACGTACTGCGCCGCCAGGGCATCACGCGGCGCAGCTTCCTGAAATTCTGCAGCCTCACCGC
CACCTCGCTCGGGCTGGGCTCCGCCGCCGCGCCGCAGATCGCCCACGCACTGGAAACCAAGCCGCGCACCCCGGTGCTGT
GGCTGCACGGGCTGGAATGCACCTGCTGCTCGGAATCCTTCATCCGCTCGGCGCATCCGCTGACCAAGGACGTGGTGCTG
TCGATGCTGTCGCTCGACTACGACGACACCCTGATGGCCGCCGCCGGCCACCAGGCCGAGGCGATCCTCGACGAGATCCG
CAAGAAGTACAAAGGCAACTACATCCTCGCGGTGGAAGGCAACCCGCCGCTCAACGAAGACGGCATGTTCGCGATCCAGG
GCGGCCGCCCGTTCGTGGAAAAGCTGCGCGAGACCGCCGCCGACTGCAAGGCGATCATCTCCTGGGGTTCCTGTGCGTCC
TGGGGCTGCGTGCAGGCCGCCAAGCCCAACCCGACCCGCGCGACGCCGGTGCACAAGGTCATCACCGACAAGCCCATCGT
CAAGGTGCCCGGCTGCCCGCCGATCGCCGAGGTGATGACCGCGGTGGTCACCTACATGCTGACCTTCGACAAGCTGCCCG
AACTGGACCGCATGGGCCGGCCGAAGATGTTCTACGGCCAGCGCATCCACGACAAATGCTACCGCCGCCCGCACTTCGAC
GCCGGCCAGTTCGCCGAAGCCTGGGACGACGAGGGGTCGCGCAAGGGCTACTGCCTGTACAAGATGGGCTGCAAGGGCCC
CACCACCTATAACGCCTGCTCCACCGTGCGCTGGAACGAAGGCGTGTCCTGGCCGGTGCAGTCCGGCCACGGCTGCATCG
GCTGCTCGGAAGACGGCTTCTGGGACAAGGGCAGCTTCTACGACCGCGTCACCGACATCAAACAATTCGGCATCGAAGCC
AACGCCGACAAGGTCGGCGCCACCGCCGCCGGCGTGGTCGGCGCGAGCGTGGCCGCGCATGCCGCGGTCACCGCGCTGGC
ACGCGCGCGCAGCAAGACCGGCATCGACGTGAAGCAAGGGGAAGAATGA

Upstream 100 bases:

>100_bases
CCTTAGGTCATTCCAACCCGCTGGCACGGAACTTGTTGTTGTCGCCACCACGCCCGCCGCAACGAAGCGGCGGACCTACG
AATGCAGGGGGTACAAAACA

Downstream 100 bases:

>100_bases
ACATGGGCGTGTACGAAACTCAGGGCTTCAAGCTCGACAACACCGGCAAGCGCGTCGTCGTCGATCCGGTCACCCGCATC
GAAGGCCACATGCGCGTCGA

Product: ferredoxin hydrogenase small subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTETFYDVLRRQGITRRSFLKFCSLTATSLGLGSAAAPQIAHALETKPRTPVLWLHGLECTCCSESFIRSAHPLTKDVVL
SMLSLDYDDTLMAAAGHQAEAILDEIRKKYKGNYILAVEGNPPLNEDGMFAIQGGRPFVEKLRETAADCKAIISWGSCAS
WGCVQAAKPNPTRATPVHKVITDKPIVKVPGCPPIAEVMTAVVTYMLTFDKLPELDRMGRPKMFYGQRIHDKCYRRPHFD
AGQFAEAWDDEGSRKGYCLYKMGCKGPTTYNACSTVRWNEGVSWPVQSGHGCIGCSEDGFWDKGSFYDRVTDIKQFGIEA
NADKVGATAAGVVGASVAAHAAVTALARARSKTGIDVKQGEE

Sequences:

>Translated_362_residues
MTETFYDVLRRQGITRRSFLKFCSLTATSLGLGSAAAPQIAHALETKPRTPVLWLHGLECTCCSESFIRSAHPLTKDVVL
SMLSLDYDDTLMAAAGHQAEAILDEIRKKYKGNYILAVEGNPPLNEDGMFAIQGGRPFVEKLRETAADCKAIISWGSCAS
WGCVQAAKPNPTRATPVHKVITDKPIVKVPGCPPIAEVMTAVVTYMLTFDKLPELDRMGRPKMFYGQRIHDKCYRRPHFD
AGQFAEAWDDEGSRKGYCLYKMGCKGPTTYNACSTVRWNEGVSWPVQSGHGCIGCSEDGFWDKGSFYDRVTDIKQFGIEA
NADKVGATAAGVVGASVAAHAAVTALARARSKTGIDVKQGEE
>Mature_361_residues
TETFYDVLRRQGITRRSFLKFCSLTATSLGLGSAAAPQIAHALETKPRTPVLWLHGLECTCCSESFIRSAHPLTKDVVLS
MLSLDYDDTLMAAAGHQAEAILDEIRKKYKGNYILAVEGNPPLNEDGMFAIQGGRPFVEKLRETAADCKAIISWGSCASW
GCVQAAKPNPTRATPVHKVITDKPIVKVPGCPPIAEVMTAVVTYMLTFDKLPELDRMGRPKMFYGQRIHDKCYRRPHFDA
GQFAEAWDDEGSRKGYCLYKMGCKGPTTYNACSTVRWNEGVSWPVQSGHGCIGCSEDGFWDKGSFYDRVTDIKQFGIEAN
ADKVGATAAGVVGASVAAHAAVTALARARSKTGIDVKQGEE

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=329, Percent_Identity=73.2522796352584, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI1789371, Length=294, Percent_Identity=44.2176870748299, Blast_Score=276, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 39498; Mature: 39367

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETFYDVLRRQGITRRSFLKFCSLTATSLGLGSAAAPQIAHALETKPRTPVLWLHGLEC
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCCC
TCCSESFIRSAHPLTKDVVLSMLSLDYDDTLMAAAGHQAEAILDEIRKKYKGNYILAVEG
CHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEEECC
NPPLNEDGMFAIQGGRPFVEKLRETAADCKAIISWGSCASWGCVQAAKPNPTRATPVHKV
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
ITDKPIVKVPGCPPIAEVMTAVVTYMLTFDKLPELDRMGRPKMFYGQRIHDKCYRRPHFD
HCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHCCHHHHHHHCCCCCC
AGQFAEAWDDEGSRKGYCLYKMGCKGPTTYNACSTVRWNEGVSWPVQSGHGCIGCSEDGF
CCHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCEEECCCCCC
WDKGSFYDRVTDIKQFGIEANADKVGATAAGVVGASVAAHAAVTALARARSKTGIDVKQG
CCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EE
CC
>Mature Secondary Structure 
TETFYDVLRRQGITRRSFLKFCSLTATSLGLGSAAAPQIAHALETKPRTPVLWLHGLEC
CHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCCC
TCCSESFIRSAHPLTKDVVLSMLSLDYDDTLMAAAGHQAEAILDEIRKKYKGNYILAVEG
CHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHCCCEEEEECC
NPPLNEDGMFAIQGGRPFVEKLRETAADCKAIISWGSCASWGCVQAAKPNPTRATPVHKV
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHH
ITDKPIVKVPGCPPIAEVMTAVVTYMLTFDKLPELDRMGRPKMFYGQRIHDKCYRRPHFD
HCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHCCHHHHHHHCCCCCC
AGQFAEAWDDEGSRKGYCLYKMGCKGPTTYNACSTVRWNEGVSWPVQSGHGCIGCSEDGF
CCHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCEEECCCCCC
WDKGSFYDRVTDIKQFGIEANADKVGATAAGVVGASVAAHAAVTALARARSKTGIDVKQG
CCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1383192; 12948488; 2493816 [H]