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The map label for this gene is ampR [H]

Identifier: 119900038

GI number: 119900038

Start: 4104611

End: 4105519

Strand: Direct

Name: ampR [H]

Synonym: azo3749

Alternate gene names: 119900038

Gene position: 4104611-4105519 (Clockwise)

Preceding gene: 119900035

Following gene: 119900039

Centisome position: 93.8

GC content: 73.6

Gene sequence:

>909_bases
ATGAATAACCCGCTTCTCCACCTGCCCCCGCTGGATCCGCTGCGCGGCTTTGTCGTGGCCGCGCGGCTGCGCTCCTTCAC
GCGCGCGGCGGCGGAGCTGTGCCTCACGCAGTCCGCGATCAGCCGTCAGGTCCAGACGCTGGAAGCAGCGCTCGGCACGC
CGCTGTTCGTCCGCGGCGTCCGCAGCCTGACGCTGACGCCCGCCGGGGCCCGCCTCGCGGCGGCGGCCGAGGCCTGGTTG
GCCGACTACGCGACGCTTGCGGTCGAACTGCGGGCGCCCGGCCCGCGCCCGGTCACCGTCACCGCCTCGATCGGCATCTC
CTCGCTGTGGCTGGTGCCGCGGCTGCGCGAGTTCCAGGCCCGCCATCCGGATACCGAAGTGCGCATCGCCGCCGGCAATC
GCGTGGTCGACCTGGCGCGCGAGGACATCGACCTCGCGCTGCGCTACTGCGGCGACCACGACGCCCCCGCGGGCGCGACC
CGCCTGTTCGGCGAAACCCTGTTTCCGGTCGCCCACCCGTCGATCGCCGCCGCCATCGAGGCGCTCGACGCCGCCACGCT
GCCGCTGCAGACCCTGCTCGACTACGACGAACCGGGGTTTCCGTGGATACGCTGGGAACACTGGCTGGGCAGCCACGGCC
TGGCGCATGTGCGGCCGCGCCAGCGCATCGGCTACAGCCACTACGACCAGCTGATCCACGCCGCCGCCGCGGGCCAGGGC
ATCGCGCTCGGCCGCGCGGTGCTGGTCGATCCGATGCTGGAGGACGGCCGGCTGGTGATGGTGGGGGACGAACGGCTCCC
GATCGCCGGTCGCGGCTTCTGGCTGGTGCCGGCCCCGCGGCCGATGCGGCCCGAGGTGGCACGCTTTGCGGAATGGGTGC
GCGAAACCGCGGCGGCCACCGCGCGATGA

Upstream 100 bases:

>100_bases
TGCCATCAAGTGCGTCTCAGCATTGACCTTGAAGGCAGTATCGTCATCGCCTAAGCTCGCCACAAACGGTCAATTTGCAT
TTCATCCATGCGCAAACCGC

Downstream 100 bases:

>100_bases
TGTCCGCAATGGTCCGCATCCTGTCCGGATCACCCCTGTCTGCCTCGGCTGGCAAGGCCGATGATGGCGCCACCTTCAAC
CAGACGGAGTCCCGCAATGT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MNNPLLHLPPLDPLRGFVVAARLRSFTRAAAELCLTQSAISRQVQTLEAALGTPLFVRGVRSLTLTPAGARLAAAAEAWL
ADYATLAVELRAPGPRPVTVTASIGISSLWLVPRLREFQARHPDTEVRIAAGNRVVDLAREDIDLALRYCGDHDAPAGAT
RLFGETLFPVAHPSIAAAIEALDAATLPLQTLLDYDEPGFPWIRWEHWLGSHGLAHVRPRQRIGYSHYDQLIHAAAAGQG
IALGRAVLVDPMLEDGRLVMVGDERLPIAGRGFWLVPAPRPMRPEVARFAEWVRETAAATAR

Sequences:

>Translated_302_residues
MNNPLLHLPPLDPLRGFVVAARLRSFTRAAAELCLTQSAISRQVQTLEAALGTPLFVRGVRSLTLTPAGARLAAAAEAWL
ADYATLAVELRAPGPRPVTVTASIGISSLWLVPRLREFQARHPDTEVRIAAGNRVVDLAREDIDLALRYCGDHDAPAGAT
RLFGETLFPVAHPSIAAAIEALDAATLPLQTLLDYDEPGFPWIRWEHWLGSHGLAHVRPRQRIGYSHYDQLIHAAAAGQG
IALGRAVLVDPMLEDGRLVMVGDERLPIAGRGFWLVPAPRPMRPEVARFAEWVRETAAATAR
>Mature_302_residues
MNNPLLHLPPLDPLRGFVVAARLRSFTRAAAELCLTQSAISRQVQTLEAALGTPLFVRGVRSLTLTPAGARLAAAAEAWL
ADYATLAVELRAPGPRPVTVTASIGISSLWLVPRLREFQARHPDTEVRIAAGNRVVDLAREDIDLALRYCGDHDAPAGAT
RLFGETLFPVAHPSIAAAIEALDAATLPLQTLLDYDEPGFPWIRWEHWLGSHGLAHVRPRQRIGYSHYDQLIHAAAAGQG
IALGRAVLVDPMLEDGRLVMVGDERLPIAGRGFWLVPAPRPMRPEVARFAEWVRETAAATAR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=35.3535353535354, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788706, Length=312, Percent_Identity=29.4871794871795, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786448, Length=293, Percent_Identity=27.3037542662116, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787589, Length=265, Percent_Identity=27.5471698113208, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081978, Length=270, Percent_Identity=29.2592592592593, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI145693193, Length=135, Percent_Identity=32.5925925925926, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786401, Length=263, Percent_Identity=25.8555133079848, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789440, Length=247, Percent_Identity=27.1255060728745, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790208, Length=126, Percent_Identity=35.7142857142857, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI157672245, Length=165, Percent_Identity=29.6969696969697, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32853; Mature: 32853

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNPLLHLPPLDPLRGFVVAARLRSFTRAAAELCLTQSAISRQVQTLEAALGTPLFVRGV
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RSLTLTPAGARLAAAAEAWLADYATLAVELRAPGPRPVTVTASIGISSLWLVPRLREFQA
HHEEECCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHH
RHPDTEVRIAAGNRVVDLAREDIDLALRYCGDHDAPAGATRLFGETLFPVAHPSIAAAIE
CCCCCEEEEECCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
ALDAATLPLQTLLDYDEPGFPWIRWEHWLGSHGLAHVRPRQRIGYSHYDQLIHAAAAGQG
HHHHHHCCHHHHHCCCCCCCCEEEEEHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCC
IALGRAVLVDPMLEDGRLVMVGDERLPIAGRGFWLVPAPRPMRPEVARFAEWVRETAAAT
HHHCHHHEECCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
AR
CC
>Mature Secondary Structure
MNNPLLHLPPLDPLRGFVVAARLRSFTRAAAELCLTQSAISRQVQTLEAALGTPLFVRGV
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
RSLTLTPAGARLAAAAEAWLADYATLAVELRAPGPRPVTVTASIGISSLWLVPRLREFQA
HHEEECCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHH
RHPDTEVRIAAGNRVVDLAREDIDLALRYCGDHDAPAGATRLFGETLFPVAHPSIAAAIE
CCCCCEEEEECCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
ALDAATLPLQTLLDYDEPGFPWIRWEHWLGSHGLAHVRPRQRIGYSHYDQLIHAAAAGQG
HHHHHHCCHHHHHCCCCCCCCEEEEEHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCC
IALGRAVLVDPMLEDGRLVMVGDERLPIAGRGFWLVPAPRPMRPEVARFAEWVRETAAAT
HHHCHHHEECCCCCCCEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
AR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]