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The map label for this gene is amtB [H]

Identifier: 119900029

GI number: 119900029

Start: 4095436

End: 4096842

Strand: Direct

Name: amtB [H]

Synonym: azo3740

Alternate gene names: 119900029

Gene position: 4095436-4096842 (Clockwise)

Preceding gene: 119900028

Following gene: 119900030

Centisome position: 93.59

GC content: 67.24

Gene sequence:

>1407_bases
ATGAAAAAACTATTCGCGATCCTGCTGACGGCCTTAACCGTCGGCTTTGCGGGCGGCGTTATCGCCCAGGAAAACACGGC
GGCGCCGACTGCTCCGGCCGCCGTCGAGGCGCCGGCCGCCACCGACGCCGCCCCGGCTGCAGCTGAAGCCCCGGCCGCCG
CCGAAGAAGTCGTGGCCACGGTCAACAAGGGCGACGTCGCCTGGATCATGACCGCCACCCTGCTGGTGCTGTTCATGGCC
CTGCCCGGCCTCGCGCTGTTCTACGGCGGCCTCGTCCGCACCAAGAACATGCTGTCGGTGCTGATGCAGGTGATGGTGGT
GTTCTCGCTGATCGCCGTGCTGTGGGCCTTCTACGGCTACAGCCTGGCCTTCACCGAGGCCAGCCCCTTCATCGGCTCGC
TCGACAAGCTGTTCCTGTCCGGGGTGAGCAACAGCACCCTCGCCGACACCTTCAGCGCCGATGCGAAGCTGCCGGAGTAC
GCCTTCATCGCCTTCCAGGCCACCTTCGCGGGCATCACCTGCGCGCTGATCGTCGGCTCCTTCGCCGAACGCATCAAGTT
CTCCGCGGTGCTGCTGTTCTCGGTGATCTGGTTCACCTTCTGCTACCTGCCGATCTGCCACATGGTGTGGGGCCCGGGCG
GCTTCCTGCTGGGTGACGGCGCGCTCGACTTCGCCGGCGGTACCGTGGTGCACATCAACGCCGGTGTCGCCGGTCTGGTG
GGTGCCTACATGGTCGGCAAGCGCATCGGCTTCGGTCGCGAATCCATGGCTCCGCACTCGCTGACGCTGACCATGGTCGG
TGCCTCGATGCTGTGGGTGGGCTGGTTCGGCTTCAACGCCGGCTCCAACCTGGAAGCCACCGCGGGTGCCGCGCTCGCCT
TCATCAACACCCTGCTCGCCACCGCCGCCGCCGTGCTGGCCTGGTCGCTGGGCGAAGCGATGTTCAAGGGCAAGCCCTCG
ATGCTGGGTGCGGCCTCCGGCGCGGTTGCCGGCCTGGTGGCGATCACCCCGGCTTGCGGCTCGGTCGGCCCGATGGGCGC
GATCATCATCGGCCTGCTCGCCGGCTTCGTCTGCCTGTGGGGCGTCAATGGCCTCAAGCGCATGCTCGGCGCGGATGACT
CGCTCGACGTGTTCGGCGTGCATGGCCTCGGCGGCATCCTCGGCGCCATCCTGACCGGCGTGTTCACCGCGCCGTCGCTC
GGCGGCACCGGTGCCGAAGACTTCGCGATGGGCCACCAGGTGTGGGTGCAGACCTACTCGGTGCTGATCACCATCGTCTG
GTCCGCCGTGGTCGCCTTCATCGGCTACAAGATCGCCGACATGTTCGTCGGTCTGCGGGTGCCGGAAGAAGAGGAACGCG
AAGGCCTGGACGTGACCGCCCACGGCGAAACCGCGTACCACTACTAA

Upstream 100 bases:

>100_bases
AGATCGGTGACGGCAAGATCTTTGTCTTCGACCTGGAGCAGGCGATCCGCATCCGCACCGGAGAAACCGGGGCCGACGCC
CTGTAACGGAGAATCAAACA

Downstream 100 bases:

>100_bases
GCACGCTCTACCGGGTCAGGACCGCCTCTCCTCCGGTCCTGCCTCATCGGGCGCCCTGCGGGGCGCCCTTTTTCGTTTAC
CGCGCCCGCTCCTGCGCCAC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MKKLFAILLTALTVGFAGGVIAQENTAAPTAPAAVEAPAATDAAPAAAEAPAAAEEVVATVNKGDVAWIMTATLLVLFMA
LPGLALFYGGLVRTKNMLSVLMQVMVVFSLIAVLWAFYGYSLAFTEASPFIGSLDKLFLSGVSNSTLADTFSADAKLPEY
AFIAFQATFAGITCALIVGSFAERIKFSAVLLFSVIWFTFCYLPICHMVWGPGGFLLGDGALDFAGGTVVHINAGVAGLV
GAYMVGKRIGFGRESMAPHSLTLTMVGASMLWVGWFGFNAGSNLEATAGAALAFINTLLATAAAVLAWSLGEAMFKGKPS
MLGAASGAVAGLVAITPACGSVGPMGAIIIGLLAGFVCLWGVNGLKRMLGADDSLDVFGVHGLGGILGAILTGVFTAPSL
GGTGAEDFAMGHQVWVQTYSVLITIVWSAVVAFIGYKIADMFVGLRVPEEEEREGLDVTAHGETAYHY

Sequences:

>Translated_468_residues
MKKLFAILLTALTVGFAGGVIAQENTAAPTAPAAVEAPAATDAAPAAAEAPAAAEEVVATVNKGDVAWIMTATLLVLFMA
LPGLALFYGGLVRTKNMLSVLMQVMVVFSLIAVLWAFYGYSLAFTEASPFIGSLDKLFLSGVSNSTLADTFSADAKLPEY
AFIAFQATFAGITCALIVGSFAERIKFSAVLLFSVIWFTFCYLPICHMVWGPGGFLLGDGALDFAGGTVVHINAGVAGLV
GAYMVGKRIGFGRESMAPHSLTLTMVGASMLWVGWFGFNAGSNLEATAGAALAFINTLLATAAAVLAWSLGEAMFKGKPS
MLGAASGAVAGLVAITPACGSVGPMGAIIIGLLAGFVCLWGVNGLKRMLGADDSLDVFGVHGLGGILGAILTGVFTAPSL
GGTGAEDFAMGHQVWVQTYSVLITIVWSAVVAFIGYKIADMFVGLRVPEEEEREGLDVTAHGETAYHY
>Mature_468_residues
MKKLFAILLTALTVGFAGGVIAQENTAAPTAPAAVEAPAATDAAPAAAEAPAAAEEVVATVNKGDVAWIMTATLLVLFMA
LPGLALFYGGLVRTKNMLSVLMQVMVVFSLIAVLWAFYGYSLAFTEASPFIGSLDKLFLSGVSNSTLADTFSADAKLPEY
AFIAFQATFAGITCALIVGSFAERIKFSAVLLFSVIWFTFCYLPICHMVWGPGGFLLGDGALDFAGGTVVHINAGVAGLV
GAYMVGKRIGFGRESMAPHSLTLTMVGASMLWVGWFGFNAGSNLEATAGAALAFINTLLATAAAVLAWSLGEAMFKGKPS
MLGAASGAVAGLVAITPACGSVGPMGAIIIGLLAGFVCLWGVNGLKRMLGADDSLDVFGVHGLGGILGAILTGVFTAPSL
GGTGAEDFAMGHQVWVQTYSVLITIVWSAVVAFIGYKIADMFVGLRVPEEEEREGLDVTAHGETAYHY

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=410, Percent_Identity=59.2682926829268, Blast_Score=480, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17550182, Length=446, Percent_Identity=28.47533632287, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17550180, Length=433, Percent_Identity=29.0993071593533, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI133931100, Length=448, Percent_Identity=27.2321428571429, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32564042, Length=424, Percent_Identity=26.8867924528302, Blast_Score=117, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6325396, Length=424, Percent_Identity=35.1415094339623, Blast_Score=220, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=435, Percent_Identity=33.3333333333333, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6324187, Length=422, Percent_Identity=35.0710900473934, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI161078314, Length=419, Percent_Identity=27.4463007159904, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 48276; Mature: 48276

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFAILLTALTVGFAGGVIAQENTAAPTAPAAVEAPAATDAAPAAAEAPAAAEEVVAT
CHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VNKGDVAWIMTATLLVLFMALPGLALFYGGLVRTKNMLSVLMQVMVVFSLIAVLWAFYGY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SLAFTEASPFIGSLDKLFLSGVSNSTLADTFSADAKLPEYAFIAFQATFAGITCALIVGS
EEEECCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
FAERIKFSAVLLFSVIWFTFCYLPICHMVWGPGGFLLGDGALDFAGGTVVHINAGVAGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCEEEEECCCHHHHH
GAYMVGKRIGFGRESMAPHSLTLTMVGASMLWVGWFGFNAGSNLEATAGAALAFINTLLA
HHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
TAAAVLAWSLGEAMFKGKPSMLGAASGAVAGLVAITPACGSVGPMGAIIIGLLAGFVCLW
HHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
GVNGLKRMLGADDSLDVFGVHGLGGILGAILTGVFTAPSLGGTGAEDFAMGHQVWVQTYS
CHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
VLITIVWSAVVAFIGYKIADMFVGLRVPEEEEREGLDVTAHGETAYHY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEECCCCCCCC
>Mature Secondary Structure
MKKLFAILLTALTVGFAGGVIAQENTAAPTAPAAVEAPAATDAAPAAAEAPAAAEEVVAT
CHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
VNKGDVAWIMTATLLVLFMALPGLALFYGGLVRTKNMLSVLMQVMVVFSLIAVLWAFYGY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SLAFTEASPFIGSLDKLFLSGVSNSTLADTFSADAKLPEYAFIAFQATFAGITCALIVGS
EEEECCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHH
FAERIKFSAVLLFSVIWFTFCYLPICHMVWGPGGFLLGDGALDFAGGTVVHINAGVAGLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCEEEEECCCHHHHH
GAYMVGKRIGFGRESMAPHSLTLTMVGASMLWVGWFGFNAGSNLEATAGAALAFINTLLA
HHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
TAAAVLAWSLGEAMFKGKPSMLGAASGAVAGLVAITPACGSVGPMGAIIIGLLAGFVCLW
HHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
GVNGLKRMLGADDSLDVFGVHGLGGILGAILTGVFTAPSLGGTGAEDFAMGHQVWVQTYS
CHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
VLITIVWSAVVAFIGYKIADMFVGLRVPEEEEREGLDVTAHGETAYHY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]