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The map label for this gene is livM5 [H]

Identifier: 119900021

GI number: 119900021

Start: 4088347

End: 4089444

Strand: Direct

Name: livM5 [H]

Synonym: azo3732

Alternate gene names: 119900021

Gene position: 4088347-4089444 (Clockwise)

Preceding gene: 119900020

Following gene: 119900022

Centisome position: 93.43

GC content: 66.03

Gene sequence:

>1098_bases
ATGAACGAACTCGCCGCCGCCATTCCCTGGCTGGGCAAACGCAACCCGCTCGCCGCGCGCTGGCTGATCCTGATCCTCGC
CATCGCCGCGCCGCTGATCGCGTGGCAGTTCGGCCGCAGCTGGGTACGCATCCTGGACTTCGCGCTGCTCTACATGATGT
TGGCGCTGGGCCTCAACCTGGTGGTGGGCTTCGCCGGCCTGCTCGACCTCGGCTACATCGCCTTCTACGCGGTCGGCGCC
TACAGCTTCGCCTTCCTCGCCTCGCCGCACTTCGACGTGCATCTGCCGTTCTGGCTGATCCTGCCGCTGGGCGCCGGTTT
CGCCGCGCTCGCCGGCATCGCGCTCGGCTTTCCGGTGCTGAGGTTACGCGGCGACTACCTCGCCATCGTCACCCTCGGCT
TCGGCGAGATCGTGCGCATCTTCATGCTCAACCTGAACTACCCGATCAACATCACCAACGGGCCGCAGGGCATCAACATG
ATCGACCCGATCGTGCTGTTCGGCTGGGACCTCTCACGCAACATCCGCGTCACCGAAGAACTCTCGATCAACTCGCTGTT
CCTCTACTACTACTTCTTCCTCGCCTTCGTCGCCGGCTCCATCATCTTCATCCGCCGCCTGCAGATCTCGCGCATCGGCC
GCGCGTGGGCGGCGATGCGCGACGACGAACTCGCCGCCAAGGCGATCGGCATCAACACCCGCAACATGAAACTGCTCGCG
TTCGCACTCGGCGCCACCTTCGGCGGCGTCTCGGGCTGCCTGTTCGGCGCCTTCCAGGGCTTCGTGTCGCCCGAATCGTT
CTCGCTGATGGAGTCGATCGCGGTGCTGACGATGGTGGTGTTCGGCGGCATGGGCAACATCGCCGGGGCGCTGGTCGGCG
CCTTCGTGCTTGCGCTGCTGCCCGAAATCCTGCGCCACATCGCGGTGCCGCTGCAGCAGGCGGTGTTCGGCCACGTGCTG
CTCGACCCCGAGGTGCTGCGCATGCTGTTGCTGTCGCTGGCGATGATCCTGATGATGCTGCTGCGTCCGGCGGGTCTGAT
CCCGGCGCGGGCGCGCTACGCGCATCCGGAAAACCTGCACCGGAGCGCCGCCGAATGA

Upstream 100 bases:

>100_bases
GGACATCTTCGCCTTCCTGATCCTCGGCCTGGTGCTGATCTTCCGGCCCACCGGGCTGCTCGGCGAACGCGTGTCCGACC
GCGCGTAAGGGGAAAGAACC

Downstream 100 bases:

>100_bases
TCACGCTGCTCGAAGCCCGCAACATCGGCAAGCGCTTCGGCGGCCTGCAGGCGCTCACCGACGTCTCGCTGACCATCCGC
AAGGGCGAGGTGTATGGCCT

Product: putative branched-chain amino acid transport permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MNELAAAIPWLGKRNPLAARWLILILAIAAPLIAWQFGRSWVRILDFALLYMMLALGLNLVVGFAGLLDLGYIAFYAVGA
YSFAFLASPHFDVHLPFWLILPLGAGFAALAGIALGFPVLRLRGDYLAIVTLGFGEIVRIFMLNLNYPINITNGPQGINM
IDPIVLFGWDLSRNIRVTEELSINSLFLYYYFFLAFVAGSIIFIRRLQISRIGRAWAAMRDDELAAKAIGINTRNMKLLA
FALGATFGGVSGCLFGAFQGFVSPESFSLMESIAVLTMVVFGGMGNIAGALVGAFVLALLPEILRHIAVPLQQAVFGHVL
LDPEVLRMLLLSLAMILMMLLRPAGLIPARARYAHPENLHRSAAE

Sequences:

>Translated_365_residues
MNELAAAIPWLGKRNPLAARWLILILAIAAPLIAWQFGRSWVRILDFALLYMMLALGLNLVVGFAGLLDLGYIAFYAVGA
YSFAFLASPHFDVHLPFWLILPLGAGFAALAGIALGFPVLRLRGDYLAIVTLGFGEIVRIFMLNLNYPINITNGPQGINM
IDPIVLFGWDLSRNIRVTEELSINSLFLYYYFFLAFVAGSIIFIRRLQISRIGRAWAAMRDDELAAKAIGINTRNMKLLA
FALGATFGGVSGCLFGAFQGFVSPESFSLMESIAVLTMVVFGGMGNIAGALVGAFVLALLPEILRHIAVPLQQAVFGHVL
LDPEVLRMLLLSLAMILMMLLRPAGLIPARARYAHPENLHRSAAE
>Mature_365_residues
MNELAAAIPWLGKRNPLAARWLILILAIAAPLIAWQFGRSWVRILDFALLYMMLALGLNLVVGFAGLLDLGYIAFYAVGA
YSFAFLASPHFDVHLPFWLILPLGAGFAALAGIALGFPVLRLRGDYLAIVTLGFGEIVRIFMLNLNYPINITNGPQGINM
IDPIVLFGWDLSRNIRVTEELSINSLFLYYYFFLAFVAGSIIFIRRLQISRIGRAWAAMRDDELAAKAIGINTRNMKLLA
FALGATFGGVSGCLFGAFQGFVSPESFSLMESIAVLTMVVFGGMGNIAGALVGAFVLALLPEILRHIAVPLQQAVFGHVL
LDPEVLRMLLLSLAMILMMLLRPAGLIPARARYAHPENLHRSAAE

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=331, Percent_Identity=43.8066465256798, Blast_Score=220, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 39924; Mature: 39924

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELAAAIPWLGKRNPLAARWLILILAIAAPLIAWQFGRSWVRILDFALLYMMLALGLNL
CCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVGFAGLLDLGYIAFYAVGAYSFAFLASPHFDVHLPFWLILPLGAGFAALAGIALGFPVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHCCCHHHHHHHHHCCHHH
RLRGDYLAIVTLGFGEIVRIFMLNLNYPINITNGPQGINMIDPIVLFGWDLSRNIRVTEE
HCCCCEEEEEEECHHHHHHHHHHCCCCCEEECCCCCCCCHHCHHHHEECCCCCCCEEEEC
LSINSLFLYYYFFLAFVAGSIIFIRRLQISRIGRAWAAMRDDELAAKAIGINTRNMKLLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHH
FALGATFGGVSGCLFGAFQGFVSPESFSLMESIAVLTMVVFGGMGNIAGALVGAFVLALL
HHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
PEILRHIAVPLQQAVFGHVLLDPEVLRMLLLSLAMILMMLLRPAGLIPARARYAHPENLH
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHH
RSAAE
HCCCC
>Mature Secondary Structure
MNELAAAIPWLGKRNPLAARWLILILAIAAPLIAWQFGRSWVRILDFALLYMMLALGLNL
CCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVGFAGLLDLGYIAFYAVGAYSFAFLASPHFDVHLPFWLILPLGAGFAALAGIALGFPVL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHCCCHHHHHHHHHCCHHH
RLRGDYLAIVTLGFGEIVRIFMLNLNYPINITNGPQGINMIDPIVLFGWDLSRNIRVTEE
HCCCCEEEEEEECHHHHHHHHHHCCCCCEEECCCCCCCCHHCHHHHEECCCCCCCEEEEC
LSINSLFLYYYFFLAFVAGSIIFIRRLQISRIGRAWAAMRDDELAAKAIGINTRNMKLLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHH
FALGATFGGVSGCLFGAFQGFVSPESFSLMESIAVLTMVVFGGMGNIAGALVGAFVLALL
HHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
PEILRHIAVPLQQAVFGHVLLDPEVLRMLLLSLAMILMMLLRPAGLIPARARYAHPENLH
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHH
RSAAE
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]