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The map label for this gene is btuF [H]

Identifier: 119899809

GI number: 119899809

Start: 3862136

End: 3863032

Strand: Direct

Name: btuF [H]

Synonym: azo3520

Alternate gene names: 119899809

Gene position: 3862136-3863032 (Clockwise)

Preceding gene: 119899808

Following gene: 119899811

Centisome position: 88.26

GC content: 69.01

Gene sequence:

>897_bases
ATGCACCGACGACTTGCCGCAACCGCCCTTGCCTGCACCCTTGCAGGACTCCCCGCCGCCGCCGTCGCGGACATCCGCAT
CACCGACGATGCCGGACGGGAGGTGGTGCTGCCGCGGCCGGCACAACGCATCGTCAGCCTCGCGCCGCACGTCACCGAAC
TCCTGTTTGCCGCCGGCGCCGCGCCGCGCGTGGTGGGCGTGGTCAGCTACAGCGACTTCCCCGATGCCGCGCGCCAGCTA
CCGAAGGTGGGCGGCTATGCGAGCGTCGACCTCGAAGCGGTCGTCGCGCTGCGGCCGGACCTGGTGATCGCGTGGAAGAG
CGGCAACCGCAACACGCACCTCGACCGCCTGACCGCTCTCGGCATTCCGGTATTCATCAATGAACCGCGCCGCCTGGACG
ATGTGGCCGGCAGCCTGCGCTCGCTCGGCCGCCTCGCGGGCACCGACGAAGTGGCCGAACGCGCCGCCAGCGCCTTTGCC
GCGCGGCGCGATGCACTGCGGAACCGCTACGCCGAGCGGCCGAAGGTGCGGATGCTGTACCAGATCTGGAACCAGCCGCT
GATGACGGTCAATGGCGAGCACCTGATTTCGGACGTGATCAGGCTGTGCGGAGGCGACAACGTGTTCGCCGGCTTGCCTC
AACTCGCGCCGACGATAGGCGTGGAAGCGGTGCTGGCGGCGAACCCGGAGGTCGTCGTGGCAAGCGGCATGGGCGAAGCC
CGGCCGGAATGGCTGGAACAATGGAAGCGTTGGCCGGCACTGACCGCGAACGCCGCAAACAACCTCTACTTCATCCCGCC
GGAACTGGTGCAGCGCCACACGCCACGCATCCTGGATGGCGCCCAGCGCTTGTGCGAGCAGCTGGAGGAAGCACGCGCCA
AACGCCCGCCGCGCTAA

Upstream 100 bases:

>100_bases
CCGCCAGCCTGCGCTTCGGCCTTCCCGCAACCGAAACCGAGTGGCGCCGGCTCGGCACCGCGCTGGGCGGTCTGCACGAC
CTGATCCGACAAGGATGAAC

Downstream 100 bases:

>100_bases
GCCGCTTCAGGGCGTGCGCGAACGCCCCTGACTGCGGCGGTGCTGCCGCGCCTCGAGCCACCAGACGCGATCGAACACCC
GCGCCAGACTGGGCAGCAGC

Product: putative vitamin B12 transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MHRRLAATALACTLAGLPAAAVADIRITDDAGREVVLPRPAQRIVSLAPHVTELLFAAGAAPRVVGVVSYSDFPDAARQL
PKVGGYASVDLEAVVALRPDLVIAWKSGNRNTHLDRLTALGIPVFINEPRRLDDVAGSLRSLGRLAGTDEVAERAASAFA
ARRDALRNRYAERPKVRMLYQIWNQPLMTVNGEHLISDVIRLCGGDNVFAGLPQLAPTIGVEAVLAANPEVVVASGMGEA
RPEWLEQWKRWPALTANAANNLYFIPPELVQRHTPRILDGAQRLCEQLEEARAKRPPR

Sequences:

>Translated_298_residues
MHRRLAATALACTLAGLPAAAVADIRITDDAGREVVLPRPAQRIVSLAPHVTELLFAAGAAPRVVGVVSYSDFPDAARQL
PKVGGYASVDLEAVVALRPDLVIAWKSGNRNTHLDRLTALGIPVFINEPRRLDDVAGSLRSLGRLAGTDEVAERAASAFA
ARRDALRNRYAERPKVRMLYQIWNQPLMTVNGEHLISDVIRLCGGDNVFAGLPQLAPTIGVEAVLAANPEVVVASGMGEA
RPEWLEQWKRWPALTANAANNLYFIPPELVQRHTPRILDGAQRLCEQLEEARAKRPPR
>Mature_298_residues
MHRRLAATALACTLAGLPAAAVADIRITDDAGREVVLPRPAQRIVSLAPHVTELLFAAGAAPRVVGVVSYSDFPDAARQL
PKVGGYASVDLEAVVALRPDLVIAWKSGNRNTHLDRLTALGIPVFINEPRRLDDVAGSLRSLGRLAGTDEVAERAASAFA
ARRDALRNRYAERPKVRMLYQIWNQPLMTVNGEHLISDVIRLCGGDNVFAGLPQLAPTIGVEAVLAANPEVVVASGMGEA
RPEWLEQWKRWPALTANAANNLYFIPPELVQRHTPRILDGAQRLCEQLEEARAKRPPR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=250, Percent_Identity=34.4, Blast_Score=152, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 32407; Mature: 32407

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRRLAATALACTLAGLPAAAVADIRITDDAGREVVLPRPAQRIVSLAPHVTELLFAAGA
CCCHHHHHHHHHHHHCCCHHHEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHCCC
APRVVGVVSYSDFPDAARQLPKVGGYASVDLEAVVALRPDLVIAWKSGNRNTHLDRLTAL
CCCEEEEEECCCCCHHHHHCCCCCCCCCCCHHHHEEECCCEEEEECCCCCCCHHHHHHHH
GIPVFINEPRRLDDVAGSLRSLGRLAGTDEVAERAASAFAARRDALRNRYAERPKVRMLY
CCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
QIWNQPLMTVNGEHLISDVIRLCGGDNVFAGLPQLAPTIGVEAVLAANPEVVVASGMGEA
HHHCCCCEEECHHHHHHHHHHHHCCCCHHHCCHHHHHHHCCCEEEECCCCEEEECCCCCC
RPEWLEQWKRWPALTANAANNLYFIPPELVQRHTPRILDGAQRLCEQLEEARAKRPPR
CHHHHHHHHHCCCEECCCCCCEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHRRLAATALACTLAGLPAAAVADIRITDDAGREVVLPRPAQRIVSLAPHVTELLFAAGA
CCCHHHHHHHHHHHHCCCHHHEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHCCC
APRVVGVVSYSDFPDAARQLPKVGGYASVDLEAVVALRPDLVIAWKSGNRNTHLDRLTAL
CCCEEEEEECCCCCHHHHHCCCCCCCCCCCHHHHEEECCCEEEEECCCCCCCHHHHHHHH
GIPVFINEPRRLDDVAGSLRSLGRLAGTDEVAERAASAFAARRDALRNRYAERPKVRMLY
CCCEEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
QIWNQPLMTVNGEHLISDVIRLCGGDNVFAGLPQLAPTIGVEAVLAANPEVVVASGMGEA
HHHCCCCEEECHHHHHHHHHHHHCCCCHHHCCHHHHHHHCCCEEEECCCCEEEECCCCCC
RPEWLEQWKRWPALTANAANNLYFIPPELVQRHTPRILDGAQRLCEQLEEARAKRPPR
CHHHHHHHHHCCCEECCCCCCEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA