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The map label for this gene is prpC [H]

Identifier: 119776427

GI number: 119776427

Start: 3915169

End: 3916305

Strand: Direct

Name: prpC [H]

Synonym: Sama_3295

Alternate gene names: 119776427

Gene position: 3915169-3916305 (Clockwise)

Preceding gene: 119776426

Following gene: 119776428

Centisome position: 90.92

GC content: 58.49

Gene sequence:

>1137_bases
ATGGCTGAGACAACCGATAAAAAACTGAGTGGTGCAGGCCTGCGTGGTCAGAGCGCCGGTGAAACCGCCCTGAGCACAGT
AGGTAAATCCGGCTCAGGCCTCACCTACCGTGGCTACGACGTAAAAGATCTGGCCGAAAACGCCAGCTTCGAAGAAGTGG
CCTTTCTCATTCTGTACGGCGAGCTGCCCACTCAACTGGAGCTGGACAACTACCGCGCCAAGCTCAAAGGCCTGCGCGGC
CTGCCACAGGCACTGAAAGAAGTGCTGGAGCGCATCCCCGCCAATGCCCATCCCATGGATGTGCTGCGTACCGGCTGCTC
TATGCTGGGCAACCTGGAAACCGAACACAGCTTTGCCGACCAGTTTGATGTAACCAACCGCATGCTGGCGGTGTTCCCGT
CCATCATCTGCTACTGGTATCGTTTCAGCCACGATGGCGTGCGCATCGAAACCGAGACCGATGACGAGCAAATCGGTGCC
CACTTCCTGCACCTGCTGCACGGCAAGGCGCCTTCTGCCCTGCATGCCCGGGTAATGGATGTATCCCTCATCCTCTACGC
AGAGCACGAGTTCAACGCCTCTACCTTTACTGCCCGCGTCTGTGCCTCGACCCTGTCTGATATGCACTCTTGCGTAACCG
GTGCCATCGGTTCACTGCGCGGCCCGCTGCACGGCGGCGCCAACGAGGCGGCCATGGAGCTTATCCAGGACATGCGCGAT
GAGCAGCACGCCCGCGACGTACTGGCCGGTATGCTGGAGCGCAAAGAAAAAATCATGGGCTTTGGTCACGCCATCTACCG
CGAATCCGACCCTCGTAACGCCATCATCAAAGAATGGTCTGAAAAGCTCGCCAAGGAATATGGCGACGACCGTCTGTACC
GTGTTTCCGTGGCCTGCGAAGCCTTTATGTGGGAACAAAAGAAGCTCTTCTGTAACGCCGACTTCTTCCATGCCAGCGCC
TATCACTTCATGGGCATCCCCACCAAGCTGTTCACCCCCATATTCGTATGTTCACGGGTGAGCGGCTGGACTGCCCACGT
GATGGAGCAGCGCAGCAACAACCGCATCATCCGCCCAAGCGCCGATTATGTGGGTGTGGAACTGCGCAGCGTGACCCCAA
TCAGCGAGCGCGGCTGA

Upstream 100 bases:

>100_bases
AATCCGTGGCGAACAAAGTAATCCATAAATCAGCGCCGGGACCCCATCGCCCTACCGATGCCGGCAACCAAGAACAAACG
AGTTTACGAGGGAATGCAAA

Downstream 100 bases:

>100_bases
TAACAGCGGGGCCGACGAGCAGTCGGCCCCAATAACGCCCGCCGCACAAAAACAACAGCCACTCATTATTCGCGCCCTTT
TGCCTGTCTGGAACGCCGTT

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MAETTDKKLSGAGLRGQSAGETALSTVGKSGSGLTYRGYDVKDLAENASFEEVAFLILYGELPTQLELDNYRAKLKGLRG
LPQALKEVLERIPANAHPMDVLRTGCSMLGNLETEHSFADQFDVTNRMLAVFPSIICYWYRFSHDGVRIETETDDEQIGA
HFLHLLHGKAPSALHARVMDVSLILYAEHEFNASTFTARVCASTLSDMHSCVTGAIGSLRGPLHGGANEAAMELIQDMRD
EQHARDVLAGMLERKEKIMGFGHAIYRESDPRNAIIKEWSEKLAKEYGDDRLYRVSVACEAFMWEQKKLFCNADFFHASA
YHFMGIPTKLFTPIFVCSRVSGWTAHVMEQRSNNRIIRPSADYVGVELRSVTPISERG

Sequences:

>Translated_378_residues
MAETTDKKLSGAGLRGQSAGETALSTVGKSGSGLTYRGYDVKDLAENASFEEVAFLILYGELPTQLELDNYRAKLKGLRG
LPQALKEVLERIPANAHPMDVLRTGCSMLGNLETEHSFADQFDVTNRMLAVFPSIICYWYRFSHDGVRIETETDDEQIGA
HFLHLLHGKAPSALHARVMDVSLILYAEHEFNASTFTARVCASTLSDMHSCVTGAIGSLRGPLHGGANEAAMELIQDMRD
EQHARDVLAGMLERKEKIMGFGHAIYRESDPRNAIIKEWSEKLAKEYGDDRLYRVSVACEAFMWEQKKLFCNADFFHASA
YHFMGIPTKLFTPIFVCSRVSGWTAHVMEQRSNNRIIRPSADYVGVELRSVTPISERG
>Mature_377_residues
AETTDKKLSGAGLRGQSAGETALSTVGKSGSGLTYRGYDVKDLAENASFEEVAFLILYGELPTQLELDNYRAKLKGLRGL
PQALKEVLERIPANAHPMDVLRTGCSMLGNLETEHSFADQFDVTNRMLAVFPSIICYWYRFSHDGVRIETETDDEQIGAH
FLHLLHGKAPSALHARVMDVSLILYAEHEFNASTFTARVCASTLSDMHSCVTGAIGSLRGPLHGGANEAAMELIQDMRDE
QHARDVLAGMLERKEKIMGFGHAIYRESDPRNAIIKEWSEKLAKEYGDDRLYRVSVACEAFMWEQKKLFCNADFFHASAY
HFMGIPTKLFTPIFVCSRVSGWTAHVMEQRSNNRIIRPSADYVGVELRSVTPISERG

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=382, Percent_Identity=22.5130890052356, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786527, Length=374, Percent_Identity=51.3368983957219, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1786939, Length=380, Percent_Identity=29.4736842105263, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17555174, Length=359, Percent_Identity=22.5626740947075, Blast_Score=90, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6319850, Length=387, Percent_Identity=23.2558139534884, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324328, Length=366, Percent_Identity=22.9508196721311, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6325257, Length=348, Percent_Identity=24.1379310344828, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640126, Length=392, Percent_Identity=23.469387755102, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24640124, Length=392, Percent_Identity=23.469387755102, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356863, Length=399, Percent_Identity=21.5538847117794, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 42161; Mature: 42030

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETTDKKLSGAGLRGQSAGETALSTVGKSGSGLTYRGYDVKDLAENASFEEVAFLILYG
CCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCCHHHHHHCCCHHHEEEEEEEC
ELPTQLELDNYRAKLKGLRGLPQALKEVLERIPANAHPMDVLRTGCSMLGNLETEHSFAD
CCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
QFDVTNRMLAVFPSIICYWYRFSHDGVRIETETDDEQIGAHFLHLLHGKAPSALHARVMD
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHE
VSLILYAEHEFNASTFTARVCASTLSDMHSCVTGAIGSLRGPLHGGANEAAMELIQDMRD
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
EQHARDVLAGMLERKEKIMGFGHAIYRESDPRNAIIKEWSEKLAKEYGDDRLYRVSVACE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHH
AFMWEQKKLFCNADFFHASAYHFMGIPTKLFTPIFVCSRVSGWTAHVMEQRSNNRIIRPS
HHHHHHHHHEECCCHHHCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEECCC
ADYVGVELRSVTPISERG
CCEEEEEEEECCCCCCCC
>Mature Secondary Structure 
AETTDKKLSGAGLRGQSAGETALSTVGKSGSGLTYRGYDVKDLAENASFEEVAFLILYG
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEECCCCHHHHHHCCCHHHEEEEEEEC
ELPTQLELDNYRAKLKGLRGLPQALKEVLERIPANAHPMDVLRTGCSMLGNLETEHSFAD
CCCCCEEHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
QFDVTNRMLAVFPSIICYWYRFSHDGVRIETETDDEQIGAHFLHLLHGKAPSALHARVMD
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHE
VSLILYAEHEFNASTFTARVCASTLSDMHSCVTGAIGSLRGPLHGGANEAAMELIQDMRD
EEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
EQHARDVLAGMLERKEKIMGFGHAIYRESDPRNAIIKEWSEKLAKEYGDDRLYRVSVACE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHHH
AFMWEQKKLFCNADFFHASAYHFMGIPTKLFTPIFVCSRVSGWTAHVMEQRSNNRIIRPS
HHHHHHHHHEECCCHHHCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEECCC
ADYVGVELRSVTPISERG
CCEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]