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The map label for this gene is prpB [H]

Identifier: 119776426

GI number: 119776426

Start: 3914095

End: 3914979

Strand: Direct

Name: prpB [H]

Synonym: Sama_3294

Alternate gene names: 119776426

Gene position: 3914095-3914979 (Clockwise)

Preceding gene: 119776425

Following gene: 119776427

Centisome position: 90.9

GC content: 58.42

Gene sequence:

>885_bases
ATGACCCACAGCGCAGGCAAGCGTTTTCGCGACGCACTGGCACGCTCCAAGCCACTGCAAATCGTAGGTACCACCAACGC
CTATTTCGCTCTGATGGCCGAAAAGACCGGCTTTCAGGCTCTCTATCTGTCGGGCGCCGGGGTGGCTAACGCCTCTTATG
GTCTGCCGGATCTGGGCATGACCTCCATGAATGATGTGCTGATTGATGCCGGCCGCATCACCTCGGCCACCCAGCTGCCA
CTGCTGGTCGACATAGACACCGGCTGGGGCGGCGCCTTCAACATCGCCCGCACCATCAAGGAATTTGAAAAGGTTGGTGT
TGCCGCGGTGCATATGGAAGACCAGGTGTCGCAAAAGCGTTGCGGCCACCGCCCCAACAAGGCCGTGGTGAGCACCGAAG
AGATGGTTGACCGCATCAAGGCCGCCGTGGATGCCCGCACCGATGAGAACTTCGTCATCATGGCACGCACCGACGCGGTA
GCCGTTGAAGGGCTGGATGCCGGTATAGAGCGTGCCCAGGCCTATATCGAGGCCGGTGCCGACATGATTTTTGCCGAGGC
CCTGACCGAGCTGCCACAGTACCGCAAGTTCAAGGACGCGGTAAAAGCGCCCATCCTCGCCAATATGACCGAATTCGGTC
AAACCGAGCTGTTCGACAAGTCAGCCCTGTATGAAGCCGGAGCCGATATGGTGCTCTATCCACTCGGCACCTTCCGCGCC
GCCAACCAGGCCGCCCTTAAGGTGATGCAGGCACTGATGAACGATGGCCACCAGCGCAATGTGCTCAGCACCATGCAAAG
CCGGAAAGAGCTGTACGAGTTTTTGAACTACCACAGCTTTGAAGACACGCTGGACCGCCTCTTTGCTGAAGGCAAAAACA
AATAA

Upstream 100 bases:

>100_bases
ACACAGAGGCACGTCTTAAAGAACTCAAGGCCGAGGCAAGCGCCCGTGAGCACAGCTCGCTTACCTTCGCCAATACACAG
CAATTAAAGGGAGAGACAGC

Downstream 100 bases:

>100_bases
GGCCGCAGGCCTGAACAAGTAAAAGACAAGCAAGGACGAAGACAGCGGGGCATAAGGCCCAGGCAGTAGCTGCTTCCCGC
CCCGGCAAAAATCCGTGGCG

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTHSAGKRFRDALARSKPLQIVGTTNAYFALMAEKTGFQALYLSGAGVANASYGLPDLGMTSMNDVLIDAGRITSATQLP
LLVDIDTGWGGAFNIARTIKEFEKVGVAAVHMEDQVSQKRCGHRPNKAVVSTEEMVDRIKAAVDARTDENFVIMARTDAV
AVEGLDAGIERAQAYIEAGADMIFAEALTELPQYRKFKDAVKAPILANMTEFGQTELFDKSALYEAGADMVLYPLGTFRA
ANQAALKVMQALMNDGHQRNVLSTMQSRKELYEFLNYHSFEDTLDRLFAEGKNK

Sequences:

>Translated_294_residues
MTHSAGKRFRDALARSKPLQIVGTTNAYFALMAEKTGFQALYLSGAGVANASYGLPDLGMTSMNDVLIDAGRITSATQLP
LLVDIDTGWGGAFNIARTIKEFEKVGVAAVHMEDQVSQKRCGHRPNKAVVSTEEMVDRIKAAVDARTDENFVIMARTDAV
AVEGLDAGIERAQAYIEAGADMIFAEALTELPQYRKFKDAVKAPILANMTEFGQTELFDKSALYEAGADMVLYPLGTFRA
ANQAALKVMQALMNDGHQRNVLSTMQSRKELYEFLNYHSFEDTLDRLFAEGKNK
>Mature_293_residues
THSAGKRFRDALARSKPLQIVGTTNAYFALMAEKTGFQALYLSGAGVANASYGLPDLGMTSMNDVLIDAGRITSATQLPL
LVDIDTGWGGAFNIARTIKEFEKVGVAAVHMEDQVSQKRCGHRPNKAVVSTEEMVDRIKAAVDARTDENFVIMARTDAVA
VEGLDAGIERAQAYIEAGADMIFAEALTELPQYRKFKDAVKAPILANMTEFGQTELFDKSALYEAGADMVLYPLGTFRAA
NQAALKVMQALMNDGHQRNVLSTMQSRKELYEFLNYHSFEDTLDRLFAEGKNK

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=293, Percent_Identity=61.7747440273038, Blast_Score=373, Evalue=1e-105,
Organism=Escherichia coli, GI1790445, Length=248, Percent_Identity=27.4193548387097, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32236; Mature: 32105

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSAGKRFRDALARSKPLQIVGTTNAYFALMAEKTGFQALYLSGAGVANASYGLPDLGM
CCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCC
TSMNDVLIDAGRITSATQLPLLVDIDTGWGGAFNIARTIKEFEKVGVAAVHMEDQVSQKR
CCHHHHHEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHH
CGHRPNKAVVSTEEMVDRIKAAVDARTDENFVIMARTDAVAVEGLDAGIERAQAYIEAGA
CCCCCCCCEECHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEHHHHHHHHHHHHHHHHCC
DMIFAEALTELPQYRKFKDAVKAPILANMTEFGQTELFDKSALYEAGADMVLYPLGTFRA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCHHHC
ANQAALKVMQALMNDGHQRNVLSTMQSRKELYEFLNYHSFEDTLDRLFAEGKNK
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
THSAGKRFRDALARSKPLQIVGTTNAYFALMAEKTGFQALYLSGAGVANASYGLPDLGM
CCCHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCC
TSMNDVLIDAGRITSATQLPLLVDIDTGWGGAFNIARTIKEFEKVGVAAVHMEDQVSQKR
CCHHHHHEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEHHHHHHHHH
CGHRPNKAVVSTEEMVDRIKAAVDARTDENFVIMARTDAVAVEGLDAGIERAQAYIEAGA
CCCCCCCCEECHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEHHHHHHHHHHHHHHHHCC
DMIFAEALTELPQYRKFKDAVKAPILANMTEFGQTELFDKSALYEAGADMVLYPLGTFRA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCHHHC
ANQAALKVMQALMNDGHQRNVLSTMQSRKELYEFLNYHSFEDTLDRLFAEGKNK
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]