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The map label for this gene is oprF [H]

Identifier: 119776394

GI number: 119776394

Start: 3870944

End: 3871549

Strand: Direct

Name: oprF [H]

Synonym: Sama_3262

Alternate gene names: 119776394

Gene position: 3870944-3871549 (Clockwise)

Preceding gene: 119776393

Following gene: 119776395

Centisome position: 89.89

GC content: 50.99

Gene sequence:

>606_bases
ATGAGAATGACTTTATTGATGTTGGCTCTGCTGAGCCTTGGCGCTTGTTCTATGCGCGATACCGTGAGTATGGATCAGCC
TACAACACAGGTATTTGATCTGAACGATCCAGACCGTGACGGCGTAATCGAAGCCCGTGAACGTTGTGCTGGCACCGTAA
AGGGTGCCGCTATCGACAACTACGGCTGCGGTGATATCAAAACCATCAACGAACGTCGTGAGCTCAAGGTATTGTTCCCC
AACGACTCATCCTACCTTGCCCCACAATACTTCGGCCAAATTGAAGAGCTGGCGTCGTTCATGCGTCAGTTTCCCAATAC
TAAAGTGACCATTGAAGGTCACTGCAGCCGAACCGGCGGCTACGACTACAACATGGAGCTGTCTCAGCGCCGTGCCAACG
CCGTCACTGAAGTATTAACACAGCAATATGGCATCGAGTCCAGTCGCCTCAAAGCTGTGGGATTTGGCTACAACAGCCCG
GTTGACCCAAGTGACACCCCTGAAGCACACGATAAAAACCGTCGGGTAATCGCGGAGATTGTTGGAGACGACACTACTGC
CGACATGAAGTGGAACATATATACTGTCGACCAGGAAGTGAAATAA

Upstream 100 bases:

>100_bases
ATCGGGTACTGAATGCCACCGGTCGCCTGTTGGATTCACTGAAGGTAACCAGACCCACGGTATGGCAGGGAGAGCGTGAA
TATGAAGGGGGAGTAAAACC

Downstream 100 bases:

>100_bases
CATTTGAATCAATAGCATGAAAGGCGGACAAACGTTTACCCAAACAATGCTTAAGGTCTGCCAACACAGCCTGTTGGCAA
CGGCCATATTGGGAACTTGT

Product: OmpA/MotB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MRMTLLMLALLSLGACSMRDTVSMDQPTTQVFDLNDPDRDGVIEARERCAGTVKGAAIDNYGCGDIKTINERRELKVLFP
NDSSYLAPQYFGQIEELASFMRQFPNTKVTIEGHCSRTGGYDYNMELSQRRANAVTEVLTQQYGIESSRLKAVGFGYNSP
VDPSDTPEAHDKNRRVIAEIVGDDTTADMKWNIYTVDQEVK

Sequences:

>Translated_201_residues
MRMTLLMLALLSLGACSMRDTVSMDQPTTQVFDLNDPDRDGVIEARERCAGTVKGAAIDNYGCGDIKTINERRELKVLFP
NDSSYLAPQYFGQIEELASFMRQFPNTKVTIEGHCSRTGGYDYNMELSQRRANAVTEVLTQQYGIESSRLKAVGFGYNSP
VDPSDTPEAHDKNRRVIAEIVGDDTTADMKWNIYTVDQEVK
>Mature_201_residues
MRMTLLMLALLSLGACSMRDTVSMDQPTTQVFDLNDPDRDGVIEARERCAGTVKGAAIDNYGCGDIKTINERRELKVLFP
NDSSYLAPQYFGQIEELASFMRQFPNTKVTIEGHCSRTGGYDYNMELSQRRANAVTEVLTQQYGIESSRLKAVGFGYNSP
VDPSDTPEAHDKNRRVIAEIVGDDTTADMKWNIYTVDQEVK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=100, Percent_Identity=36, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786962, Length=88, Percent_Identity=37.5, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 22507; Mature: 22507

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMTLLMLALLSLGACSMRDTVSMDQPTTQVFDLNDPDRDGVIEARERCAGTVKGAAIDN
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCEECC
YGCGDIKTINERRELKVLFPNDSSYLAPQYFGQIEELASFMRQFPNTKVTIEGHCSRTGG
CCCCCCEECCCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
YDYNMELSQRRANAVTEVLTQQYGIESSRLKAVGFGYNSPVDPSDTPEAHDKNRRVIAEI
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEE
VGDDTTADMKWNIYTVDQEVK
CCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MRMTLLMLALLSLGACSMRDTVSMDQPTTQVFDLNDPDRDGVIEARERCAGTVKGAAIDN
CHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCCCCEECC
YGCGDIKTINERRELKVLFPNDSSYLAPQYFGQIEELASFMRQFPNTKVTIEGHCSRTGG
CCCCCCEECCCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
YDYNMELSQRRANAVTEVLTQQYGIESSRLKAVGFGYNSPVDPSDTPEAHDKNRRVIAEI
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEE
VGDDTTADMKWNIYTVDQEVK
CCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]