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The map label for this gene is cysD

Identifier: 119776023

GI number: 119776023

Start: 3433525

End: 3434433

Strand: Reverse

Name: cysD

Synonym: Sama_2891

Alternate gene names: 119776023

Gene position: 3434433-3433525 (Counterclockwise)

Preceding gene: 119776024

Following gene: 119776022

Centisome position: 79.76

GC content: 55.23

Gene sequence:

>909_bases
ATGTCAGGGCCTGTGTTAAGTCACCTTGAGCAACTGGAAGCGGAAAGCATCCAGATCTTCCGTGAAGTCGCAGCGGAGTT
TGATAACCCTGTGATGCTGTATTCCATCGGTAAGGATTCCTCTGTGCTGCTGCATTTGGCGCGCAAGGCCTTCTATCCCG
GCAAAATTCCCTTTCCCCTGCTGCATGTGGATACCGACTGGAAATTCCGCGAGATGATTAAGTTTCGCGACGAAGCGGCA
GTGAAGTACGGCTTTGATCTGCTGGTGCACAAAAACCCCGAAGGCCTTGCCATGGGCATGAACCCCTTCACCTTTGGTTC
GTCCAAGCACACAGACGTGATGAAGACCGAGGGTCTGAAACAGGCGCTCAACAAATACGGTTTCGATGCCGCCTTCGGTG
GTGCGCGCCGCGATGAAGAAAAGTCCCGCGCCAAGGAGCGCGTGTATTCCTTCCGCGACAGCAAGCACCGCTGGGACCCG
AAAAACCAGCGCCCCGAGCTGTGGCACACCTACAACGGTCAGGTGAACAAGGGCGAAAGCATTCGTGTGTTCCCGCTGTC
CAACTGGACCGAACTGGATATCTGGCAATACATCTATCAGGAAAACATCGACATAGTGCCCTTGTACTTTGCCGATTACC
GTCCAGTGGTTGAGCGTAACGGCACCCTGATCATGGTTGACGATGAGCGTATGCCGCTGAACGAAGGCGAAGTGCCCGAG
CAAAAGCTGGTGCGTTTCCGCACCCTGGGTTGCTACCCGCTTACCGGCGCTATCGAATCCAGCGCCACCACCCTCACCGG
CATCATTGAAGAGATGCTGCTGTCCCGCTCCAGTGAGCGTCAGGGCAGGGTGATTGACCACGATTCATCGGGTTCCATGG
AAAAGAAAAAGCGCGAGGGGTATTTCTGA

Upstream 100 bases:

>100_bases
CTGGTTTGAACCCGCCCAAGTGGCCGAGGTTCTGCCCAAACAGAATACGAATTGATGCTGCCACGGCAGCCTTCTCAAAT
ATAAGACTCGGAGAAACAAG

Downstream 100 bases:

>100_bases
TGTCTATTGCACAGCAAGTTAAAGATCTTGGTATTGAGCAATACCTGGAAAGACAACAGCATAAGTCGCTGCTTAAATTC
CTCACCTGTGGCTCTGTGGA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSGPVLSHLEQLEAESIQIFREVAAEFDNPVMLYSIGKDSSVLLHLARKAFYPGKIPFPLLHVDTDWKFREMIKFRDEAA
VKYGFDLLVHKNPEGLAMGMNPFTFGSSKHTDVMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERVYSFRDSKHRWDP
KNQRPELWHTYNGQVNKGESIRVFPLSNWTELDIWQYIYQENIDIVPLYFADYRPVVERNGTLIMVDDERMPLNEGEVPE
QKLVRFRTLGCYPLTGAIESSATTLTGIIEEMLLSRSSERQGRVIDHDSSGSMEKKKREGYF

Sequences:

>Translated_302_residues
MSGPVLSHLEQLEAESIQIFREVAAEFDNPVMLYSIGKDSSVLLHLARKAFYPGKIPFPLLHVDTDWKFREMIKFRDEAA
VKYGFDLLVHKNPEGLAMGMNPFTFGSSKHTDVMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERVYSFRDSKHRWDP
KNQRPELWHTYNGQVNKGESIRVFPLSNWTELDIWQYIYQENIDIVPLYFADYRPVVERNGTLIMVDDERMPLNEGEVPE
QKLVRFRTLGCYPLTGAIESSATTLTGIIEEMLLSRSSERQGRVIDHDSSGSMEKKKREGYF
>Mature_301_residues
SGPVLSHLEQLEAESIQIFREVAAEFDNPVMLYSIGKDSSVLLHLARKAFYPGKIPFPLLHVDTDWKFREMIKFRDEAAV
KYGFDLLVHKNPEGLAMGMNPFTFGSSKHTDVMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERVYSFRDSKHRWDPK
NQRPELWHTYNGQVNKGESIRVFPLSNWTELDIWQYIYQENIDIVPLYFADYRPVVERNGTLIMVDDERMPLNEGEVPEQ
KLVRFRTLGCYPLTGAIESSATTLTGIIEEMLLSRSSERQGRVIDHDSSGSMEKKKREGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=78.7878787878788, Blast_Score=500, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSD_SHEAM (A1S9N7)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928763.1
- ProteinModelPortal:   A1S9N7
- SMR:   A1S9N7
- STRING:   A1S9N7
- GeneID:   4605138
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2891
- NMPDR:   fig|326297.7.peg.2710
- eggNOG:   COG0175
- HOGENOM:   HBG758022
- OMA:   GMIRFRT
- PhylomeDB:   A1S9N7
- ProtClustDB:   PRK05253
- HAMAP:   MF_00064
- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF002936
- TIGRFAMs:   TIGR02039

Pfam domain/function: PF01507 PAPS_reduct

EC number: =2.7.7.4

Molecular weight: Translated: 34842; Mature: 34711

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPVLSHLEQLEAESIQIFREVAAEFDNPVMLYSIGKDSSVLLHLARKAFYPGKIPFPL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEE
LHVDTDWKFREMIKFRDEAAVKYGFDLLVHKNPEGLAMGMNPFTFGSSKHTDVMKTEGLK
EEECCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHH
QALNKYGFDAAFGGARRDEEKSRAKERVYSFRDSKHRWDPKNQRPELWHTYNGQVNKGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCE
IRVFPLSNWTELDIWQYIYQENIDIVPLYFADYRPVVERNGTLIMVDDERMPLNEGEVPE
EEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCEEEEECCCCCCCCCCCCCH
QKLVRFRTLGCYPLTGAIESSATTLTGIIEEMLLSRSSERQGRVIDHDSSGSMEKKKREG
HHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHCC
YF
CC
>Mature Secondary Structure 
SGPVLSHLEQLEAESIQIFREVAAEFDNPVMLYSIGKDSSVLLHLARKAFYPGKIPFPL
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEE
LHVDTDWKFREMIKFRDEAAVKYGFDLLVHKNPEGLAMGMNPFTFGSSKHTDVMKTEGLK
EEECCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHHHHHHH
QALNKYGFDAAFGGARRDEEKSRAKERVYSFRDSKHRWDPKNQRPELWHTYNGQVNKGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCE
IRVFPLSNWTELDIWQYIYQENIDIVPLYFADYRPVVERNGTLIMVDDERMPLNEGEVPE
EEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHHCCCEEEEECCCCCCCCCCCCCH
QKLVRFRTLGCYPLTGAIESSATTLTGIIEEMLLSRSSERQGRVIDHDSSGSMEKKKREG
HHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHCC
YF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA