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The map label for this gene is prfB [H]

Identifier: 119776010

GI number: 119776010

Start: 3417962

End: 3419056

Strand: Reverse

Name: prfB [H]

Synonym: Sama_2878

Alternate gene names: 119776010

Gene position: 3419056-3417962 (Counterclockwise)

Preceding gene: 119776012

Following gene: 119776009

Centisome position: 79.4

GC content: 54.06

Gene sequence:

>1095_bases
TTGAAGTCAATCCGGTTAAATTCAAAATTAAGGAGCTGGCCCAGCGCACTATGCTCCTTAGGGGGTATCTTTGACTACGA
CGCCAAAAAAGAGCGTCTGGAAGAAGTTACCCGCGAGCTTGAAAGCTCAGATGTCTGGAACAACCCCGAGCGTGCTCAGG
CACTGGGCAAAGAACGCGCCAGCCTGGAGAATGTGGTCAAGACCATAGACACTCTGGATTCGGGTCTGGAAGATGTGGAA
GGCCTGCTTGAGCTGGCCGTTGAGGAAGAAGACGAAGGCACCTTTGAAGATGCCAAGGCCGAGCTTGATGAGCTTGAGAA
GAGCCTTGAGGATCTGGAGTTCCGCCGCATGTTCTCCGGACCACAGGATCCTTCCGACTGCTATCTGGACATTCAGTCCG
GCTCCGGCGGTACCGAAGCCCAGGACTGGGCAGAAATGGTGCTGCGCATGTACCTGCGTTGGGGTGAAGCACACGACTTC
AAACCTGAATTGATTGAAGTGTCAGACGGTGACGTGGCCGGTATCAAGAGTGCCACCATCAAGTTTACCGGCGAGTATGC
CTATGGTTGGCTGCGCACAGAAACCGGTGTACATCGTTTGGTGCGTAAGTCGCCATTCGATTCCAGCGGCCGTCGTCACA
CCTCGTTCTGCTCTGTGTTTGTGTATCCTGAAATCGATGATGACATCGAGATTGATATCAACCCGGCCGATCTGCGTATC
GACGTATACCGCGCCTCGGGTGCCGGTGGTCAGCACGTAAACCGGACTGAGTCAGCGGTACGTATTACCCACTTGCCCAC
CAACACCGTGGTGCAATGTCAGAACGACCGCTCACAGCACAAGAACAAAGACACCGCCATGAAGCAGCTGAAAGCCAAGC
TTTATGAGCTGGAGCTGCAAAAGCAAAACGCCGAAAAGCAAGCCGCCGAAGATGCCAAGTCTGACATCGGCTGGGGCAGC
CAAATCCGCTCTTATGTACTGGATGACTCCCGTATCAAGGATTTGCGTACCGGCGTAGAGACCCGCAACACCCAATCGGT
GCTGGACGGCGATCTGGACAAGTTTATTGAAGCCAGCCTTAAATCCGGGCTGTAA

Upstream 100 bases:

>100_bases
TGACAAAGCCGGATGCTTAACTGGTATCCAGCCAGTCACTTTGGTAGAATACGCCGTTAATTTTTCTGTTATCCAATCGA
TTTAAGTTGAATCACCATGT

Downstream 100 bases:

>100_bases
TTTACTGCGAGAAGAGACGATGACTGAACACATCCAAGACGAGAACAAGCTGATTGCTGAACGTCGTGCCAAGCTGGAAC
ACGTCCGCAAGAACTGCCCA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKSIRLNSKLRSWPSALCSLGGIFDYDAKKERLEEVTRELESSDVWNNPERAQALGKERASLENVVKTIDTLDSGLEDVE
GLLELAVEEEDEGTFEDAKAELDELEKSLEDLEFRRMFSGPQDPSDCYLDIQSGSGGTEAQDWAEMVLRMYLRWGEAHDF
KPELIEVSDGDVAGIKSATIKFTGEYAYGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDIEIDINPADLRI
DVYRASGAGGQHVNRTESAVRITHLPTNTVVQCQNDRSQHKNKDTAMKQLKAKLYELELQKQNAEKQAAEDAKSDIGWGS
QIRSYVLDDSRIKDLRTGVETRNTQSVLDGDLDKFIEASLKSGL

Sequences:

>Translated_364_residues
MKSIRLNSKLRSWPSALCSLGGIFDYDAKKERLEEVTRELESSDVWNNPERAQALGKERASLENVVKTIDTLDSGLEDVE
GLLELAVEEEDEGTFEDAKAELDELEKSLEDLEFRRMFSGPQDPSDCYLDIQSGSGGTEAQDWAEMVLRMYLRWGEAHDF
KPELIEVSDGDVAGIKSATIKFTGEYAYGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDIEIDINPADLRI
DVYRASGAGGQHVNRTESAVRITHLPTNTVVQCQNDRSQHKNKDTAMKQLKAKLYELELQKQNAEKQAAEDAKSDIGWGS
QIRSYVLDDSRIKDLRTGVETRNTQSVLDGDLDKFIEASLKSGL
>Mature_364_residues
MKSIRLNSKLRSWPSALCSLGGIFDYDAKKERLEEVTRELESSDVWNNPERAQALGKERASLENVVKTIDTLDSGLEDVE
GLLELAVEEEDEGTFEDAKAELDELEKSLEDLEFRRMFSGPQDPSDCYLDIQSGSGGTEAQDWAEMVLRMYLRWGEAHDF
KPELIEVSDGDVAGIKSATIKFTGEYAYGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDIEIDINPADLRI
DVYRASGAGGQHVNRTESAVRITHLPTNTVVQCQNDRSQHKNKDTAMKQLKAKLYELELQKQNAEKQAAEDAKSDIGWGS
QIRSYVLDDSRIKDLRTGVETRNTQSVLDGDLDKFIEASLKSGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=307, Percent_Identity=34.2019543973941, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI34577120, Length=378, Percent_Identity=29.1005291005291, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI166795305, Length=214, Percent_Identity=35.0467289719626, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI2367172, Length=359, Percent_Identity=76.8802228412256, Blast_Score=571, Evalue=1e-164,
Organism=Escherichia coli, GI1787462, Length=347, Percent_Identity=38.6167146974063, Blast_Score=211, Evalue=6e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=217, Percent_Identity=34.5622119815668, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=33.5443037974684, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI19921226, Length=222, Percent_Identity=40.0900900900901, Blast_Score=157, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40966; Mature: 40966

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIRLNSKLRSWPSALCSLGGIFDYDAKKERLEEVTRELESSDVWNNPERAQALGKERA
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
SLENVVKTIDTLDSGLEDVEGLLELAVEEEDEGTFEDAKAELDELEKSLEDLEFRRMFSG
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PQDPSDCYLDIQSGSGGTEAQDWAEMVLRMYLRWGEAHDFKPELIEVSDGDVAGIKSATI
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEEE
KFTGEYAYGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDIEIDINPADLRI
EEECCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCEEE
DVYRASGAGGQHVNRTESAVRITHLPTNTVVQCQNDRSQHKNKDTAMKQLKAKLYELELQ
EEEEECCCCCCCCCCCCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHH
KQNAEKQAAEDAKSDIGWGSQIRSYVLDDSRIKDLRTGVETRNTQSVLDGDLDKFIEASL
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
KSGL
HCCC
>Mature Secondary Structure
MKSIRLNSKLRSWPSALCSLGGIFDYDAKKERLEEVTRELESSDVWNNPERAQALGKERA
CCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
SLENVVKTIDTLDSGLEDVEGLLELAVEEEDEGTFEDAKAELDELEKSLEDLEFRRMFSG
HHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PQDPSDCYLDIQSGSGGTEAQDWAEMVLRMYLRWGEAHDFKPELIEVSDGDVAGIKSATI
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEEE
KFTGEYAYGWLRTETGVHRLVRKSPFDSSGRRHTSFCSVFVYPEIDDDIEIDINPADLRI
EEECCEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCEEE
DVYRASGAGGQHVNRTESAVRITHLPTNTVVQCQNDRSQHKNKDTAMKQLKAKLYELELQ
EEEEECCCCCCCCCCCCCEEEEEECCCCCEEEECCCHHHHCCHHHHHHHHHHHHHHHHHH
KQNAEKQAAEDAKSDIGWGSQIRSYVLDDSRIKDLRTGVETRNTQSVLDGDLDKFIEASL
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
KSGL
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA