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The map label for this gene is lysS

Identifier: 119776009

GI number: 119776009

Start: 3416440

End: 3417942

Strand: Reverse

Name: lysS

Synonym: Sama_2877

Alternate gene names: 119776009

Gene position: 3417942-3416440 (Counterclockwise)

Preceding gene: 119776010

Following gene: 119776007

Centisome position: 79.37

GC content: 54.62

Gene sequence:

>1503_bases
ATGACTGAACACATCCAAGACGAGAACAAGCTGATTGCTGAACGTCGTGCCAAGCTGGAACACGTCCGCAAGAACTGCCC
AGCCAATGGTCACCCGAACACCTTCCGCCGTAAACACAAGGCTGCCGAGCTTCAAGCGCAGTTCGGTGACAAGACCAAGG
AAGAGCTGGAAGCCCTGGGTTTCCAAACCGCCATCGCTGGCCGCGTAATGGCCAAGCGTGGTCCTTTCCTGGTACTGCAG
GACGTGTCTGGCCGTATCCAGGCCTATGCCGATAAAGGCGTACAGGCCGACTTGAAAGACCGTTATGCCGGTCTGGATAT
CGGTGACATCATCGGCGTGGAAGGTACCCTGCATCTGTCCGGTAAGGGCGACCTCTATGTGAACATGGAACGCTACGAGC
TGCTGACCAAGGCGCTGCGCCCACTGCCTGAGAAATTCCACGGTCTGACCGACCAGGAAACCCGCTACCGCCAGCGTTAT
GTTGACCTCATCGTGAACGAAGACTCACGTGAAGCCTTCAAAATGCGCTCAAAAGTGGTTGCAGCTATCCGTAACTTCAT
GATCAAAAAAGAGTTCATGGAAGTTGAAACCCCAATGATGCACGTGATCCCAGGCGGCGCTTCTGCCCGTCCTTTCGTCA
CCCATCACAATGCGCTGGACATCGAAATGTACCTGCGTATTGCCCCCGAACTGTACCTGAAGCGTCTGGTTGTGGGCGGT
TTCGAGCGCGTGTTCGAAATCAACCGTAACTTCCGTAACGAGGGTCTGTCGCCACGCCATAACCCGGAATTCACCATGAT
GGAATTCTATATGGCCTACGCCGACTACAAGGATCTGATTGATTTGACCGAAGAGATGCTGTCCAGTATCGCCAAGGAAC
TGCTGGGTGACACCAAGCTGCCTTACGGCGAGCACGTGATTGATTTCGGCGGCCCATACACCCGTATGAGCATGCTCGAA
GCCATCAAGCACTACAACCCGGACAACGCCACCATCCAGGCCATGACCTACGAAGAAGTGAAGAACATAGACTTCATGCG
TAAGCTTGCCAAGGACGTGGGCATCGAAACCGAAACCTTCTGGAGCTGTGGTCAGCTTCTGGAAGAAATCTTTGGTGAGA
CTGCCGAGCCCAAGCTGATGCAGCCAACCTTCATCACTGGCTACCCAGCGGACATTTCGCCGCTGGCCCGTCGCAGCGAT
GGTAATGATTTCATCACCGACCGTTTTGAATTCTTCATTGGTGGACGTGAAGTGGCCAACGGCTTCAGCGAGCTTAACGA
CGCTGAAGATCAGGACAACCGCTTCAAGGCGCAGGTAGAAGCCAAGGACGCCGGTGACGATGAAGCCATGTTCTACGATG
CTGACTTCATCACAGCGCTGGAACACGGTCTGCCGCCCACTGCTGGTCAGGGCATTGGTATCGACCGTTTGGTAATGCTC
TTTACCAACACTCACACCATCCGTGACGTGATCCTGTTCCCGGCCATGCGTCCTCAGGCGTAA

Upstream 100 bases:

>100_bases
GTAGAGACCCGCAACACCCAATCGGTGCTGGACGGCGATCTGGACAAGTTTATTGAAGCCAGCCTTAAATCCGGGCTGTA
ATTTACTGCGAGAAGAGACG

Downstream 100 bases:

>100_bases
GCTGTGTGAACAAATAAAAAACCGGCCAACTGGCCGGTTTTTTTATGGGGTTAACGGTGCATTCCTGCGGGTTACAGGTA
CAGACCAATCAGCATATTGC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MTEHIQDENKLIAERRAKLEHVRKNCPANGHPNTFRRKHKAAELQAQFGDKTKEELEALGFQTAIAGRVMAKRGPFLVLQ
DVSGRIQAYADKGVQADLKDRYAGLDIGDIIGVEGTLHLSGKGDLYVNMERYELLTKALRPLPEKFHGLTDQETRYRQRY
VDLIVNEDSREAFKMRSKVVAAIRNFMIKKEFMEVETPMMHVIPGGASARPFVTHHNALDIEMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLIDLTEEMLSSIAKELLGDTKLPYGEHVIDFGGPYTRMSMLE
AIKHYNPDNATIQAMTYEEVKNIDFMRKLAKDVGIETETFWSCGQLLEEIFGETAEPKLMQPTFITGYPADISPLARRSD
GNDFITDRFEFFIGGREVANGFSELNDAEDQDNRFKAQVEAKDAGDDEAMFYDADFITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPAMRPQA

Sequences:

>Translated_500_residues
MTEHIQDENKLIAERRAKLEHVRKNCPANGHPNTFRRKHKAAELQAQFGDKTKEELEALGFQTAIAGRVMAKRGPFLVLQ
DVSGRIQAYADKGVQADLKDRYAGLDIGDIIGVEGTLHLSGKGDLYVNMERYELLTKALRPLPEKFHGLTDQETRYRQRY
VDLIVNEDSREAFKMRSKVVAAIRNFMIKKEFMEVETPMMHVIPGGASARPFVTHHNALDIEMYLRIAPELYLKRLVVGG
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLIDLTEEMLSSIAKELLGDTKLPYGEHVIDFGGPYTRMSMLE
AIKHYNPDNATIQAMTYEEVKNIDFMRKLAKDVGIETETFWSCGQLLEEIFGETAEPKLMQPTFITGYPADISPLARRSD
GNDFITDRFEFFIGGREVANGFSELNDAEDQDNRFKAQVEAKDAGDDEAMFYDADFITALEHGLPPTAGQGIGIDRLVML
FTNTHTIRDVILFPAMRPQA
>Mature_499_residues
TEHIQDENKLIAERRAKLEHVRKNCPANGHPNTFRRKHKAAELQAQFGDKTKEELEALGFQTAIAGRVMAKRGPFLVLQD
VSGRIQAYADKGVQADLKDRYAGLDIGDIIGVEGTLHLSGKGDLYVNMERYELLTKALRPLPEKFHGLTDQETRYRQRYV
DLIVNEDSREAFKMRSKVVAAIRNFMIKKEFMEVETPMMHVIPGGASARPFVTHHNALDIEMYLRIAPELYLKRLVVGGF
ERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLIDLTEEMLSSIAKELLGDTKLPYGEHVIDFGGPYTRMSMLEA
IKHYNPDNATIQAMTYEEVKNIDFMRKLAKDVGIETETFWSCGQLLEEIFGETAEPKLMQPTFITGYPADISPLARRSDG
NDFITDRFEFFIGGREVANGFSELNDAEDQDNRFKAQVEAKDAGDDEAMFYDADFITALEHGLPPTAGQGIGIDRLVMLF
TNTHTIRDVILFPAMRPQA

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=454, Percent_Identity=43.3920704845815, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI194272210, Length=454, Percent_Identity=43.3920704845815, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI45439306, Length=445, Percent_Identity=22.247191011236, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI40789249, Length=244, Percent_Identity=26.2295081967213, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI4758762, Length=433, Percent_Identity=22.4018475750577, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790571, Length=499, Percent_Identity=61.5230460921844, Blast_Score=626, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=500, Percent_Identity=60.4, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI87082379, Length=332, Percent_Identity=30.421686746988, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI1788173, Length=292, Percent_Identity=25, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=504, Percent_Identity=39.484126984127, Blast_Score=355, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI17535927, Length=504, Percent_Identity=39.484126984127, Blast_Score=354, Evalue=7e-98,
Organism=Caenorhabditis elegans, GI71994340, Length=504, Percent_Identity=39.484126984127, Blast_Score=353, Evalue=8e-98,
Organism=Caenorhabditis elegans, GI32566633, Length=310, Percent_Identity=26.4516129032258, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17551876, Length=351, Percent_Identity=24.2165242165242, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=522, Percent_Identity=41.1877394636015, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324256, Length=383, Percent_Identity=30.8093994778068, Blast_Score=170, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6323011, Length=354, Percent_Identity=26.271186440678, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6325153, Length=229, Percent_Identity=27.5109170305677, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24640849, Length=509, Percent_Identity=39.8821218074656, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640851, Length=509, Percent_Identity=39.8821218074656, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17136276, Length=462, Percent_Identity=22.9437229437229, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24584738, Length=332, Percent_Identity=25.6024096385542, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYK_SHEAM (A1S9M3)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928749.1
- ProteinModelPortal:   A1S9M3
- SMR:   A1S9M3
- STRING:   A1S9M3
- GeneID:   4605124
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2877
- NMPDR:   fig|326297.7.peg.2696
- eggNOG:   COG1190
- HOGENOM:   HBG631383
- OMA:   VDMIANP
- PhylomeDB:   A1S9M3
- ProtClustDB:   PRK00484
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 56947; Mature: 56815

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHIQDENKLIAERRAKLEHVRKNCPANGHPNTFRRKHKAAELQAQFGDKTKEELEALG
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FQTAIAGRVMAKRGPFLVLQDVSGRIQAYADKGVQADLKDRYAGLDIGDIIGVEGTLHLS
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCHHHHHCCCCHHHEECCCEEEEEC
GKGDLYVNMERYELLTKALRPLPEKFHGLTDQETRYRQRYVDLIVNEDSREAFKMRSKVV
CCCCEEEEHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AAIRNFMIKKEFMEVETPMMHVIPGGASARPFVTHHNALDIEMYLRIAPELYLKRLVVGG
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHH
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLIDLTEEMLSSIAKELLGDTKL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PYGEHVIDFGGPYTRMSMLEAIKHYNPDNATIQAMTYEEVKNIDFMRKLAKDVGIETETF
CCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCHHHHHHHHHHCCCCHHHH
WSCGQLLEEIFGETAEPKLMQPTFITGYPADISPLARRSDGNDFITDRFEFFIGGREVAN
HHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHCCCCCCHHHHHHHHHCCCHHHHH
GFSELNDAEDQDNRFKAQVEAKDAGDDEAMFYDADFITALEHGLPPTAGQGIGIDRLVML
HHHHCCCCCCCCCCEEEEEECCCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCHHHHHHH
FTNTHTIRDVILFPAMRPQA
HHCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TEHIQDENKLIAERRAKLEHVRKNCPANGHPNTFRRKHKAAELQAQFGDKTKEELEALG
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHH
FQTAIAGRVMAKRGPFLVLQDVSGRIQAYADKGVQADLKDRYAGLDIGDIIGVEGTLHLS
HHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCHHHHHCCCCHHHEECCCEEEEEC
GKGDLYVNMERYELLTKALRPLPEKFHGLTDQETRYRQRYVDLIVNEDSREAFKMRSKVV
CCCCEEEEHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AAIRNFMIKKEFMEVETPMMHVIPGGASARPFVTHHNALDIEMYLRIAPELYLKRLVVGG
HHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEECCCCEEHHHHHHHHHHHHHHHHHHHH
FERVFEINRNFRNEGLSPRHNPEFTMMEFYMAYADYKDLIDLTEEMLSSIAKELLGDTKL
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PYGEHVIDFGGPYTRMSMLEAIKHYNPDNATIQAMTYEEVKNIDFMRKLAKDVGIETETF
CCCCEEEECCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHCHHHHHHHHHHCCCCHHHH
WSCGQLLEEIFGETAEPKLMQPTFITGYPADISPLARRSDGNDFITDRFEFFIGGREVAN
HHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCHHHHCCCCCCHHHHHHHHHCCCHHHHH
GFSELNDAEDQDNRFKAQVEAKDAGDDEAMFYDADFITALEHGLPPTAGQGIGIDRLVML
HHHHCCCCCCCCCCEEEEEECCCCCCCCEEEECHHHHHHHHHCCCCCCCCCCCHHHHHHH
FTNTHTIRDVILFPAMRPQA
HHCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA