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The map label for this gene is cysI

Identifier: 119775970

GI number: 119775970

Start: 3375438

End: 3377153

Strand: Reverse

Name: cysI

Synonym: Sama_2838

Alternate gene names: 119775970

Gene position: 3377153-3375438 (Counterclockwise)

Preceding gene: 119775971

Following gene: 119775969

Centisome position: 78.43

GC content: 53.79

Gene sequence:

>1716_bases
ATGAGCCAGTATCCAATTAAGCCTGAACTGCAGGTGCAGGGCCAGTTGTCTGATAACGAGCGCCTGAAAGCTGAAAGCAA
CCACTTGCGTGGCACTATCACCACAGATTTAAAAGACGAAATTACCGGTGGTTTTACCGGTGATAACTTCATGTTGGTGC
GTTTTCACGGCATGTATCAGCAGGATGACCGCGATTTACGGGCTGAACGCGCAGAGCAAAAGCTGGAGCCGCTGCACAAT
GTGATGCTGCGGGCGCGCATGCCCGGCGGCATTATCAAACCTGAGCAATGGCTGGCGATTGATGAGTTTGCCCGCGAGCA
CACCATGTACGGCTCCATTCGGTTAACCACCCGTCAGACCTTCCAGTTCCATGGCGTGTTTAAACCGGACATCAAGCCGA
TGCACCAGATGCTGAATAAAATTGGCATCGATTCCATTGCCACCGCCGGTGACGTAAACCGCAACGTGCTGTGTACCTCA
AACCCGGTGGAATCGGAGTTGCACCAGCAAGCCTATGAGTGGGCTGCGAAAATCTCTGAACACCTGCTGCCAAAAACCCG
CGCCTATGCCGAGATTTGGCTGGGTGAGGAAAAAGTAGAAACCACGGAAACCGAGCCGGTGCTGGGTGATAGCTACCTGC
CGCGTAAGTTTAAAACCACTGTGGTGATCCCACCGCACAACGATGTCGACGTTCATGCCAATGACCTGAACTTTGTCGCT
ATTGCCGAAAATGGCCAGTTGGTTGGTTTTAACGTGTTGGTTGGTGGCGGCCTTGCGATGACCCACGGCGATAAATCGAC
TTATCCGCGCCGGGCAGAAGATTTGGGCTTTATCGGTCTTGAGCATGTGCTGAAAGTCGCAGAGCACGTGGTGACTGTAC
AGCGTGACTATGGCGATCGGGTTAACCGCAAAAATGCCAAAACCAAATACACCCTGGATCGTATCGGCGTGGACGCCTTC
AAATCTGAGGTGGAACAACGGGTTGGCGTAGCCTTTGGCCCAAGCCGTCCTTACGAGTTTACCCACCGCGGCGATCGCTT
TGGTTGGGTGGAAGGCATTGATGGCAAGCACCATTTAACCCTGTTTATCGAAAACGGCCGTTTGCTGGATTACCCGGGTA
AGCCGCTGAAAACCGGCGTTGCTGAAATTGCCAAAGTGCATAAAGGCGATTTGCGCATGACGGCAAACCAGAACCTCATC
ATCGCGGGGGTTGCCGCTGAAGATAAAGCGCAGATTGAGCAGCTGGCGCGCGAGCATGGGTTGTTGGATAACGTCACCAC
CCAGCGCAAAAACTCGATGGCGTGCGTGTCACTGCCAACCTGTCCGCTGGCGATGGCCGAAGCCGAACGCTACTTGCCAA
CGTTGGTCACCCACGTTGAAGGCATTTTAAGCAAGCACGGCATTGCAGACGACCATATCATCCTGCGCGTCGTGGGCTGC
CCCAATGGCTGTGGCCGCGCCATGTTGGCTGAAGCCGGTTTAGTGGGCCGTGGTCCGGGCAAATACAACCTGTATCTGGG
CGGTAACACTGCCGGTACCCGTATTCCAAAATTGTATTTGGATAACGTAGCCGAAGCGGAAATTCTGGCGGCACTGGATA
GCTTGATTGGCCGCTGGTCTGTTGAGCGCAACCCAGGTGAATGTTTTGGTGATTTTGTGATTAGGGTGGGTGTTGTTGCC
GAAGTGAAAGTCAGTAAAACCGACTTCCACGCCTGA

Upstream 100 bases:

>100_bases
TACACATTGCCCAAACCCACGGTGGCTTGTCTGCCGAAGCAGCCGACGAATATTTTGAAGAGTTACGCGCGAACAAACGT
TATCAGAAGGATGTTTATTA

Downstream 100 bases:

>100_bases
GACCAGTCAGGGGGAAGCAAGGCTTCCCCCGTTTGTTTTGGCCGCTCGTTGGCCTGTGCCAAGACGTGAAGCGTCGCCAG
AACCCTTGGAGATAAGCATG

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: SiR-HP; SiRHP

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MSQYPIKPELQVQGQLSDNERLKAESNHLRGTITTDLKDEITGGFTGDNFMLVRFHGMYQQDDRDLRAERAEQKLEPLHN
VMLRARMPGGIIKPEQWLAIDEFAREHTMYGSIRLTTRQTFQFHGVFKPDIKPMHQMLNKIGIDSIATAGDVNRNVLCTS
NPVESELHQQAYEWAAKISEHLLPKTRAYAEIWLGEEKVETTETEPVLGDSYLPRKFKTTVVIPPHNDVDVHANDLNFVA
IAENGQLVGFNVLVGGGLAMTHGDKSTYPRRAEDLGFIGLEHVLKVAEHVVTVQRDYGDRVNRKNAKTKYTLDRIGVDAF
KSEVEQRVGVAFGPSRPYEFTHRGDRFGWVEGIDGKHHLTLFIENGRLLDYPGKPLKTGVAEIAKVHKGDLRMTANQNLI
IAGVAAEDKAQIEQLAREHGLLDNVTTQRKNSMACVSLPTCPLAMAEAERYLPTLVTHVEGILSKHGIADDHIILRVVGC
PNGCGRAMLAEAGLVGRGPGKYNLYLGGNTAGTRIPKLYLDNVAEAEILAALDSLIGRWSVERNPGECFGDFVIRVGVVA
EVKVSKTDFHA

Sequences:

>Translated_571_residues
MSQYPIKPELQVQGQLSDNERLKAESNHLRGTITTDLKDEITGGFTGDNFMLVRFHGMYQQDDRDLRAERAEQKLEPLHN
VMLRARMPGGIIKPEQWLAIDEFAREHTMYGSIRLTTRQTFQFHGVFKPDIKPMHQMLNKIGIDSIATAGDVNRNVLCTS
NPVESELHQQAYEWAAKISEHLLPKTRAYAEIWLGEEKVETTETEPVLGDSYLPRKFKTTVVIPPHNDVDVHANDLNFVA
IAENGQLVGFNVLVGGGLAMTHGDKSTYPRRAEDLGFIGLEHVLKVAEHVVTVQRDYGDRVNRKNAKTKYTLDRIGVDAF
KSEVEQRVGVAFGPSRPYEFTHRGDRFGWVEGIDGKHHLTLFIENGRLLDYPGKPLKTGVAEIAKVHKGDLRMTANQNLI
IAGVAAEDKAQIEQLAREHGLLDNVTTQRKNSMACVSLPTCPLAMAEAERYLPTLVTHVEGILSKHGIADDHIILRVVGC
PNGCGRAMLAEAGLVGRGPGKYNLYLGGNTAGTRIPKLYLDNVAEAEILAALDSLIGRWSVERNPGECFGDFVIRVGVVA
EVKVSKTDFHA
>Mature_570_residues
SQYPIKPELQVQGQLSDNERLKAESNHLRGTITTDLKDEITGGFTGDNFMLVRFHGMYQQDDRDLRAERAEQKLEPLHNV
MLRARMPGGIIKPEQWLAIDEFAREHTMYGSIRLTTRQTFQFHGVFKPDIKPMHQMLNKIGIDSIATAGDVNRNVLCTSN
PVESELHQQAYEWAAKISEHLLPKTRAYAEIWLGEEKVETTETEPVLGDSYLPRKFKTTVVIPPHNDVDVHANDLNFVAI
AENGQLVGFNVLVGGGLAMTHGDKSTYPRRAEDLGFIGLEHVLKVAEHVVTVQRDYGDRVNRKNAKTKYTLDRIGVDAFK
SEVEQRVGVAFGPSRPYEFTHRGDRFGWVEGIDGKHHLTLFIENGRLLDYPGKPLKTGVAEIAKVHKGDLRMTANQNLII
AGVAAEDKAQIEQLAREHGLLDNVTTQRKNSMACVSLPTCPLAMAEAERYLPTLVTHVEGILSKHGIADDHIILRVVGCP
NGCGRAMLAEAGLVGRGPGKYNLYLGGNTAGTRIPKLYLDNVAEAEILAALDSLIGRWSVERNPGECFGDFVIRVGVVAE
VKVSKTDFHA

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family

Homologues:

Organism=Escherichia coli, GI1789122, Length=553, Percent_Identity=68.5352622061483, Blast_Score=845, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322597, Length=577, Percent_Identity=44.8873483535529, Blast_Score=507, Evalue=1e-144,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): CYSI_SHEAM (A1S9I4)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928710.1
- ProteinModelPortal:   A1S9I4
- SMR:   A1S9I4
- STRING:   A1S9I4
- GeneID:   4605085
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2838
- NMPDR:   fig|326297.7.peg.2659
- eggNOG:   COG0155
- HOGENOM:   HBG626671
- OMA:   VGDEHIV
- PhylomeDB:   A1S9I4
- ProtClustDB:   PRK13504
- HAMAP:   MF_01540
- InterPro:   IPR011786
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- PRINTS:   PR00397
- TIGRFAMs:   TIGR02041

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; SSF55124 NiR_SiRalpha_1/3

EC number: =1.8.1.2

Molecular weight: Translated: 63431; Mature: 63300

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYPIKPELQVQGQLSDNERLKAESNHLRGTITTDLKDEITGGFTGDNFMLVRFHGMYQ
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCHHHHCCCCCCCCEEEEEEECCCC
QDDRDLRAERAEQKLEPLHNVMLRARMPGGIIKPEQWLAIDEFAREHTMYGSIRLTTRQT
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEEEEEEE
FQFHGVFKPDIKPMHQMLNKIGIDSIATAGDVNRNVLCTSNPVESELHQQAYEWAAKISE
EEEECCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
HLLPKTRAYAEIWLGEEKVETTETEPVLGDSYLPRKFKTTVVIPPHNDVDVHANDLNFVA
HHCCCHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCEEEEE
IAENGQLVGFNVLVGGGLAMTHGDKSTYPRRAEDLGFIGLEHVLKVAEHVVTVQRDYGDR
EECCCEEEEEEEEEECCEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
VNRKNAKTKYTLDRIGVDAFKSEVEQRVGVAFGPSRPYEFTHRGDRFGWVEGIDGKHHLT
CCCCCCCCEEEHHHHCHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCEEECCCCCEEEE
LFIENGRLLDYPGKPLKTGVAEIAKVHKGDLRMTANQNLIIAGVAAEDKAQIEQLAREHG
EEEECCEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHCC
LLDNVTTQRKNSMACVSLPTCPLAMAEAERYLPTLVTHVEGILSKHGIADDHIILRVVGC
CCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEC
PNGCGRAMLAEAGLVGRGPGKYNLYLGGNTAGTRIPKLYLDNVAEAEILAALDSLIGRWS
CCCCCCHHHHHCCCEECCCCEEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCC
VERNPGECFGDFVIRVGVVAEVKVSKTDFHA
CCCCCHHHHHHHHHHHCEEEEEEECCCCCCC
>Mature Secondary Structure 
SQYPIKPELQVQGQLSDNERLKAESNHLRGTITTDLKDEITGGFTGDNFMLVRFHGMYQ
CCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCHHHHCCCCCCCCEEEEEEECCCC
QDDRDLRAERAEQKLEPLHNVMLRARMPGGIIKPEQWLAIDEFAREHTMYGSIRLTTRQT
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEEEEEEE
FQFHGVFKPDIKPMHQMLNKIGIDSIATAGDVNRNVLCTSNPVESELHQQAYEWAAKISE
EEEECCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHH
HLLPKTRAYAEIWLGEEKVETTETEPVLGDSYLPRKFKTTVVIPPHNDVDVHANDLNFVA
HHCCCHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEECCEEEEE
IAENGQLVGFNVLVGGGLAMTHGDKSTYPRRAEDLGFIGLEHVLKVAEHVVTVQRDYGDR
EECCCEEEEEEEEEECCEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
VNRKNAKTKYTLDRIGVDAFKSEVEQRVGVAFGPSRPYEFTHRGDRFGWVEGIDGKHHLT
CCCCCCCCEEEHHHHCHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCEEECCCCCEEEE
LFIENGRLLDYPGKPLKTGVAEIAKVHKGDLRMTANQNLIIAGVAAEDKAQIEQLAREHG
EEEECCEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCHHHHHHHHHHCC
LLDNVTTQRKNSMACVSLPTCPLAMAEAERYLPTLVTHVEGILSKHGIADDHIILRVVGC
CCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEC
PNGCGRAMLAEAGLVGRGPGKYNLYLGGNTAGTRIPKLYLDNVAEAEILAALDSLIGRWS
CCCCCCHHHHHCCCEECCCCEEEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCC
VERNPGECFGDFVIRVGVVAEVKVSKTDFHA
CCCCCHHHHHHHHHHHCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA