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The map label for this gene is miaB [H]

Identifier: 119775707

GI number: 119775707

Start: 3081949

End: 3083400

Strand: Direct

Name: miaB [H]

Synonym: Sama_2575

Alternate gene names: 119775707

Gene position: 3081949-3083400 (Clockwise)

Preceding gene: 119775706

Following gene: 119775708

Centisome position: 71.57

GC content: 56.13

Gene sequence:

>1452_bases
ATGTGGTTCCAAGAGCGACGCAAACTGATGAGTAAAAAACTCCATATCAAAACCTGGGGCTGTCAGATGAATGAGTACGA
CTCATCCAAGATGGCTGACTTGCTGGGCGAATATCAGGGCTACACCTTGACCGAAGAGGCCGAGGAAGCAGACATCCTGC
TGCTCAACACCTGCTCAATTCGTGAGAAGGCGCAGGAGAAGGTGTTTCATCAGCTCGGTCGCTGGAAAACCCTGAAAGAC
AAGAATCCGGACCTTATCATCGGCGTCGGCGGCTGTGTGGCTTCTCAGGAAGGCAAGGCCATCAAGGACCGCGCCCAATG
CGTGGACATCATCTTTGGCCCCCAGACCCTGCACCGTCTGCCGGAAATGATTGACCAGGTTCGCAGCGGCGAAAAAGCCG
TGATTGACGTGTCCTTCCCGGAAATCGAGAAGTTCGACCGTCTGCCAGAACCCCGCGCCGAAGGCCCGACCGCCTTTGTA
TCCATCATGGAAGGTTGCAGCAAGTACTGCTCTTTCTGCGTGGTGCCTTACACCCGCGGTGAGGAAGTCAGCCGTCCGAT
GGATGACATCATCCTCGAAATCGCTCAGCTCGCCGAGCAAGGCGTGCGTGAAGTGAACCTGCTCGGTCAGAACGTGAACG
CCTACCGCGGCGCCAAGCATGACGGTGAAATCTGTACCTTCGCCGAACTGCTGCGTCACGTTGCCGCCATCGATGGTATC
GACCGTCTGCGCTTTACCACCAGCCACCCGATTGAATTTACTCAGGATATCATCGACGTTTACGAAGATACCCCTGAGCT
GGTGAGCTTCCTGCACCTGCCGGTACAGTCCGGTTCCGACCGGATTCTCACCGCCATGAAGCGTGGCCACATGGCTATCG
AGTACAAGTCGATTATCCGCCGCCTGCGCAAGGCCCGTCCGGACATTCAAATAAGCTCCGATTTTATCGTGGGCTTCCCC
GGCGAAACCGCCCAGGACTTCCAGGACACCATGAAGCTGATTGAAGATGTGAACTTCGACATGAGCTTCAGCTTTATCTA
CAGCGCCCGCCCGGGTACTCCGGCAGCCGATCTGCCGGATGATGTGGATATGGAAGAGAAAAAACAGCGCCTTGCTGATT
TGCAGGAACTTATCAACCAGCAGGCCATGCGTTACAGCCGTCAGATGATGGGCACAGTGCAGCGCATTCTGGTTGAAGGT
CCCTCGGTGAAGAACCCGATGGAACTGCGTGGCCGCACCGAAACCAACCGGGTGGTCAACTTTGAAGGCCTGCACAAGCA
CATAGGCACCTTCGTGGATGTGGAAATTGTGGACGTGTTCCCCAACTCACTGCGCGGCAAGTTTATCCGCGGCGAAGACG
AGATGGACCTGCGCAAGAGCCTTCGCCCGGAGGACATCCTCGCCAAGCACCAAAAGGCAGATGACTTGGGCGTGACCCAT
TTCAAGCCCTGA

Upstream 100 bases:

>100_bases
GATGCCGCCGCCTCGGGCCGTGACTAAAAAATGCCCCGATAATTGCAGGCCAACCCTAGCCACAACCAGTTTCAATGGGT
AAAATGTCGCGCTATTTTTT

Downstream 100 bases:

>100_bases
TGGCACAAACTTACAAGGGCGACTCTGGTCGCCCTTGTTATTTTAAGGCACCGCACCGCTTTGCATCACAGCCGCGAAAC
GCAGTATATTTCAGCTATAG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MWFQERRKLMSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVFHQLGRWKTLKD
KNPDLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIDQVRSGEKAVIDVSFPEIEKFDRLPEPRAEGPTAFV
SIMEGCSKYCSFCVVPYTRGEEVSRPMDDIILEIAQLAEQGVREVNLLGQNVNAYRGAKHDGEICTFAELLRHVAAIDGI
DRLRFTTSHPIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARPDIQISSDFIVGFP
GETAQDFQDTMKLIEDVNFDMSFSFIYSARPGTPAADLPDDVDMEEKKQRLADLQELINQQAMRYSRQMMGTVQRILVEG
PSVKNPMELRGRTETNRVVNFEGLHKHIGTFVDVEIVDVFPNSLRGKFIRGEDEMDLRKSLRPEDILAKHQKADDLGVTH
FKP

Sequences:

>Translated_483_residues
MWFQERRKLMSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVFHQLGRWKTLKD
KNPDLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIDQVRSGEKAVIDVSFPEIEKFDRLPEPRAEGPTAFV
SIMEGCSKYCSFCVVPYTRGEEVSRPMDDIILEIAQLAEQGVREVNLLGQNVNAYRGAKHDGEICTFAELLRHVAAIDGI
DRLRFTTSHPIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARPDIQISSDFIVGFP
GETAQDFQDTMKLIEDVNFDMSFSFIYSARPGTPAADLPDDVDMEEKKQRLADLQELINQQAMRYSRQMMGTVQRILVEG
PSVKNPMELRGRTETNRVVNFEGLHKHIGTFVDVEIVDVFPNSLRGKFIRGEDEMDLRKSLRPEDILAKHQKADDLGVTH
FKP
>Mature_483_residues
MWFQERRKLMSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVFHQLGRWKTLKD
KNPDLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIDQVRSGEKAVIDVSFPEIEKFDRLPEPRAEGPTAFV
SIMEGCSKYCSFCVVPYTRGEEVSRPMDDIILEIAQLAEQGVREVNLLGQNVNAYRGAKHDGEICTFAELLRHVAAIDGI
DRLRFTTSHPIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARPDIQISSDFIVGFP
GETAQDFQDTMKLIEDVNFDMSFSFIYSARPGTPAADLPDDVDMEEKKQRLADLQELINQQAMRYSRQMMGTVQRILVEG
PSVKNPMELRGRTETNRVVNFEGLHKHIGTFVDVEIVDVFPNSLRGKFIRGEDEMDLRKSLRPEDILAKHQKADDLGVTH
FKP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=36.1924686192469, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=36.1924686192469, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI93277076, Length=398, Percent_Identity=31.4070351758794, Blast_Score=182, Evalue=5e-46,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=77.2151898734177, Blast_Score=761, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=430, Percent_Identity=27.4418604651163, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=461, Percent_Identity=34.056399132321, Blast_Score=254, Evalue=6e-68,
Organism=Caenorhabditis elegans, GI71996771, Length=315, Percent_Identity=28.8888888888889, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=36.9515011547344, Blast_Score=291, Evalue=9e-79,
Organism=Drosophila melanogaster, GI19922432, Length=469, Percent_Identity=27.9317697228145, Blast_Score=158, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 55029; Mature: 55029

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWFQERRKLMSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSI
CCHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEECCHH
REKAQEKVFHQLGRWKTLKDKNPDLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRL
HHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHEECHHHHHHH
PEMIDQVRSGEKAVIDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCSFCVVPYTRG
HHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCC
EEVSRPMDDIILEIAQLAEQGVREVNLLGQNVNAYRGAKHDGEICTFAELLRHVAAIDGI
CHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCH
DRLRFTTSHPIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIR
HHHEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCEEEHHHHHHH
RLRKARPDIQISSDFIVGFPGETAQDFQDTMKLIEDVNFDMSFSFIYSARPGTPAADLPD
HHHHCCCCCEECCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCC
DVDMEEKKQRLADLQELINQQAMRYSRQMMGTVQRILVEGPSVKNPMELRGRTETNRVVN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEE
FEGLHKHIGTFVDVEIVDVFPNSLRGKFIRGEDEMDLRKSLRPEDILAKHQKADDLGVTH
HHHHHHHCCCCEEEEEEECCCCCCCCCEECCCCHHHHHHCCCHHHHHHHHCCCCCCCCCC
FKP
CCC
>Mature Secondary Structure
MWFQERRKLMSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSI
CCHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHCCCEECCCCCCCCEEEEECCHH
REKAQEKVFHQLGRWKTLKDKNPDLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRL
HHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHEECHHHHHHH
PEMIDQVRSGEKAVIDVSFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCSFCVVPYTRG
HHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCC
EEVSRPMDDIILEIAQLAEQGVREVNLLGQNVNAYRGAKHDGEICTFAELLRHVAAIDGI
CHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHCCH
DRLRFTTSHPIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIR
HHHEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCEEEHHHHHHH
RLRKARPDIQISSDFIVGFPGETAQDFQDTMKLIEDVNFDMSFSFIYSARPGTPAADLPD
HHHHCCCCCEECCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCC
DVDMEEKKQRLADLQELINQQAMRYSRQMMGTVQRILVEGPSVKNPMELRGRTETNRVVN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEE
FEGLHKHIGTFVDVEIVDVFPNSLRGKFIRGEDEMDLRKSLRPEDILAKHQKADDLGVTH
HHHHHHHCCCCEEEEEEECCCCCCCCCEECCCCHHHHHHCCCHHHHHHHHCCCCCCCCCC
FKP
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA