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The map label for this gene is rlmG

Identifier: 119775680

GI number: 119775680

Start: 3056722

End: 3057873

Strand: Direct

Name: rlmG

Synonym: Sama_2548

Alternate gene names: 119775680

Gene position: 3056722-3057873 (Clockwise)

Preceding gene: 119775657

Following gene: 119775685

Centisome position: 70.99

GC content: 55.12

Gene sequence:

>1152_bases
ATGACGACCCGATTTTCAGCATTCCTTTCCGGTACCCCGTGTGAGCTGGACCTTCACAGGTTTCCCGCCACCAGCGATCC
CAACCTGCAAGCCTGGGACGCCGCCGACGAACACCTGCTGAAATACCTGAATGAGTCCAACACCGAATTGGCCGCCAAAT
TTCTGGTGATCAATGACAGCTTTGGTGCCCTCACCTGCGCCCTTGCCACCGCCAGGTCCGATGCCGATATCGTCTTTGCT
GCCGATGGCCGCACCGCGCATCTGGGCTGTAAGGCAAACCTGGCATCCAATGGGCTCGCAAGCGCCAAAGTCGATTATCA
GGACTGCACAAACCTGGGCAGCTTTGCCAAGGGCAGACAGATACTGATGAAGCTGCCCAAGAATCTCAACTTCCTGACCG
ATACTTTAAGCCAGCTGAGCCAAACCCTGGCGGCGGGGGATGTGATCCTGATGGGGGCAAAGGCCAAACACATCAATCAA
TCGCTGCTTGCCTTGATTGCCAAAGCGGTGGGCCCGGCTACTGCCAGCCTCACCTGGAAAAAGACCCGCATCATTACCAT
CACCGCCGATGGCAACCCGCGTCCAGTCAGTAAGCCCAGTGTTTGGGATGTGCCAGAGCACGGCTTGAAGGTGACCAATC
TCAGTAACGTGTTCGCTGCCAGCAAACTGGATATTGGCGCCCGCTTGATGATGGCGAATCTGCCACAGGGGCATTTCAGC
TCAGTCATCGACCTTGGCTGCGGCAATGGTGTACTGGCTCTCAAGGCAGCCCAGACCTATCCGGATGCCAGACTCTATTT
GGTGGATGAGTCGGCCATGGCAGTGGAAAGTGCCCGCCAGAACTGGGCACTGAACGCCCTGGATGAGGGCCGGGCAGAAT
TTATCTGGGATGATTGTTTAAGCCACCTGCCCAATGAAGTTCAGGCAGATTTGGTCCTGTGCAACCCGCCGTTCCATCAG
GGGGAAGCCATTACTGACCATATTGCCTGGCAGATGTTTAACGATGCCAAACGCGCGTTGAAGCCGGGGGGCTTACTGCA
CATAGTTGGCAACCGCCACCTTGGTTATCACATTAAACTCAAGCGTTTATTTGGCAACTGTAAAACCATCGCCAGCAACG
GAAAGTTTGTGATTTTGCAAGCGGTAAAATAG

Upstream 100 bases:

>100_bases
ATTGGTCACATCTGCTCCTATGCTTGAGGTAACAGGAAGAGTCGCAAGCGGCACGACAATTTGCGATAATCGCGCCCCCA
CTGATGAGATACTCTGACAT

Downstream 100 bases:

>100_bases
TGCCTGACTGCGCTGATAACAATGTGTAGTGACATCGAAAAAACGCCCCTGTTATGGGGCGTTTTCGTTATCGGATAGCC
TTGGCAAAAAGGCCGGGAGG

Product: rRNA (guanine-N(2)-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G1835 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmG

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MTTRFSAFLSGTPCELDLHRFPATSDPNLQAWDAADEHLLKYLNESNTELAAKFLVINDSFGALTCALATARSDADIVFA
ADGRTAHLGCKANLASNGLASAKVDYQDCTNLGSFAKGRQILMKLPKNLNFLTDTLSQLSQTLAAGDVILMGAKAKHINQ
SLLALIAKAVGPATASLTWKKTRIITITADGNPRPVSKPSVWDVPEHGLKVTNLSNVFAASKLDIGARLMMANLPQGHFS
SVIDLGCGNGVLALKAAQTYPDARLYLVDESAMAVESARQNWALNALDEGRAEFIWDDCLSHLPNEVQADLVLCNPPFHQ
GEAITDHIAWQMFNDAKRALKPGGLLHIVGNRHLGYHIKLKRLFGNCKTIASNGKFVILQAVK

Sequences:

>Translated_383_residues
MTTRFSAFLSGTPCELDLHRFPATSDPNLQAWDAADEHLLKYLNESNTELAAKFLVINDSFGALTCALATARSDADIVFA
ADGRTAHLGCKANLASNGLASAKVDYQDCTNLGSFAKGRQILMKLPKNLNFLTDTLSQLSQTLAAGDVILMGAKAKHINQ
SLLALIAKAVGPATASLTWKKTRIITITADGNPRPVSKPSVWDVPEHGLKVTNLSNVFAASKLDIGARLMMANLPQGHFS
SVIDLGCGNGVLALKAAQTYPDARLYLVDESAMAVESARQNWALNALDEGRAEFIWDDCLSHLPNEVQADLVLCNPPFHQ
GEAITDHIAWQMFNDAKRALKPGGLLHIVGNRHLGYHIKLKRLFGNCKTIASNGKFVILQAVK
>Mature_382_residues
TTRFSAFLSGTPCELDLHRFPATSDPNLQAWDAADEHLLKYLNESNTELAAKFLVINDSFGALTCALATARSDADIVFAA
DGRTAHLGCKANLASNGLASAKVDYQDCTNLGSFAKGRQILMKLPKNLNFLTDTLSQLSQTLAAGDVILMGAKAKHINQS
LLALIAKAVGPATASLTWKKTRIITITADGNPRPVSKPSVWDVPEHGLKVTNLSNVFAASKLDIGARLMMANLPQGHFSS
VIDLGCGNGVLALKAAQTYPDARLYLVDESAMAVESARQNWALNALDEGRAEFIWDDCLSHLPNEVQADLVLCNPPFHQG
EAITDHIAWQMFNDAKRALKPGGLLHIVGNRHLGYHIKLKRLFGNCKTIASNGKFVILQAVK

Specific function: Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA

COG id: COG2813

COG function: function code J; 16S RNA G1207 methylase RsmC

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmG family

Homologues:

Organism=Escherichia coli, GI87082206, Length=374, Percent_Identity=43.8502673796791, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1790830, Length=175, Percent_Identity=35.4285714285714, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMG_SHEAM (A1S8P4)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_928420.1
- ProteinModelPortal:   A1S8P4
- STRING:   A1S8P4
- GeneID:   4604795
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_2548
- NMPDR:   fig|326297.7.peg.2388
- eggNOG:   COG2813
- HOGENOM:   HBG321931
- OMA:   NRHLGYH
- PhylomeDB:   A1S8P4
- ProtClustDB:   CLSK906089
- GO:   GO:0005737
- HAMAP:   MF_01859
- InterPro:   IPR002052
- InterPro:   IPR017237
- InterPro:   IPR007848
- PIRSF:   PIRSF037565

Pfam domain/function: PF05175 MTS

EC number: =2.1.1.174

Molecular weight: Translated: 41368; Mature: 41237

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRFSAFLSGTPCELDLHRFPATSDPNLQAWDAADEHLLKYLNESNTELAAKFLVINDS
CCCEEEHHHCCCCCEEEHEECCCCCCCCCEECCCHHHHHHHHHCCCCCEEEEEEEEEECC
FGALTCALATARSDADIVFAADGRTAHLGCKANLASNGLASAKVDYQDCTNLGSFAKGRQ
CCCEEEEEHHCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCEECHHHHCCCCHHHHHHH
ILMKLPKNLNFLTDTLSQLSQTLAAGDVILMGAKAKHINQSLLALIAKAVGPATASLTWK
HHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEE
KTRIITITADGNPRPVSKPSVWDVPEHGLKVTNLSNVFAASKLDIGARLMMANLPQGHFS
EEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCEEEEECCCCHHHH
SVIDLGCGNGVLALKAAQTYPDARLYLVDESAMAVESARQNWALNALDEGRAEFIWDDCL
HHEECCCCCCEEEEEECCCCCCCEEEEEECHHHHHHHHHCCCEECCCCCCCHHHHHHHHH
SHLPNEVQADLVLCNPPFHQGEAITDHIAWQMFNDAKRALKPGGLLHIVGNRHLGYHIKL
HHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEE
KRLFGNCKTIASNGKFVILQAVK
EHHHCCCCEEECCCCEEEEEECC
>Mature Secondary Structure 
TTRFSAFLSGTPCELDLHRFPATSDPNLQAWDAADEHLLKYLNESNTELAAKFLVINDS
CCEEEHHHCCCCCEEEHEECCCCCCCCCEECCCHHHHHHHHHCCCCCEEEEEEEEEECC
FGALTCALATARSDADIVFAADGRTAHLGCKANLASNGLASAKVDYQDCTNLGSFAKGRQ
CCCEEEEEHHCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCEECHHHHCCCCHHHHHHH
ILMKLPKNLNFLTDTLSQLSQTLAAGDVILMGAKAKHINQSLLALIAKAVGPATASLTWK
HHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEE
KTRIITITADGNPRPVSKPSVWDVPEHGLKVTNLSNVFAASKLDIGARLMMANLPQGHFS
EEEEEEEEECCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHCCCCCEEEEECCCCHHHH
SVIDLGCGNGVLALKAAQTYPDARLYLVDESAMAVESARQNWALNALDEGRAEFIWDDCL
HHEECCCCCCEEEEEECCCCCCCEEEEEECHHHHHHHHHCCCEECCCCCCCHHHHHHHHH
SHLPNEVQADLVLCNPPFHQGEAITDHIAWQMFNDAKRALKPGGLLHIVGNRHLGYHIKL
HHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEE
KRLFGNCKTIASNGKFVILQAVK
EHHHCCCCEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA