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The map label for this gene is nadE

Identifier: 119774689

GI number: 119774689

Start: 1888929

End: 1889759

Strand: Direct

Name: nadE

Synonym: Sama_1552

Alternate gene names: 119774689

Gene position: 1888929-1889759 (Clockwise)

Preceding gene: 119774686

Following gene: 119774690

Centisome position: 43.87

GC content: 53.31

Gene sequence:

>831_bases
GTGAAAGGACAAATTTTGCGTGAAATGCGGGTACTCAAAGCCATTACGCCAGACTTTGAAGTGCAGCGCCGGGTAGCTTT
TATCAAGGCCAAGCTGCGCGAAGCCCACAGCAAAACCCTGGTGTTGGGAATAAGTGGCGGGGTGGACTCATCGGTGGCCG
GTCGTTTGTGTCAACTGGCAGTCAATGAGCTCAACCAGGAAACCGACAGCCAGCAATACCGTTTTATCGCGGTCCGTTTA
CCGTACCTGGTGCAAAAAGATGAGCACGAAGCGCAAATGGCCTGTGAATTTATTCAGCCGAGCAAACTGGTCACTGTGAA
CGTAGGCAGCGGCAGCGATGGCATACACACCGAAACCCTGACAGGGTTTAAAGCTGCGGGTCTTGAATTGCCTGAAGCCA
GCAAGGTGGATTTTGTCAAAGGCAACGTCAAAGCCAGAATGCGCATGATTGCCCAGTATGAAATCGCCGGTCTCACCGGC
GGCCTGGTGGTGGGAACTGACCACAGCGCCGAAAACATCACTGGTTTTTATACCAAGTGGGGTGATGGTGCCTGCGATCT
GGCACCGCTCTTTGGCCTCAATAAGCGTCAGGTAAGACAGATTGCACGGTATCTCGGCGCTCCTGATGTGCTGGTGGTTA
AGGCTCCCACTGCTGATCTGGAATGCCATCGTCCGGGTCTGGAAGACGAAGTGGCACTGGGACTGACCTACGACCAGATT
GACGACTTTCTTGAAGGTAAAGAGGTGCCCAGGTCGGCGGAAGACAAGCTGATTGGTATTTATAAGGCTACCCAGCACAA
GCGTCAGCCCATTCCTACCATCTACGACTGA

Upstream 100 bases:

>100_bases
GATATGAAACAATTTACCCCAGTTTACGGGGCGGTCCCGGCCGCGCGCTAGAAAAATATTGCAGCGGAGCCGGGCTTGGT
TTGCGACAGCGGAGAGAAGT

Downstream 100 bases:

>100_bases
TGAAAAAAGCCGGCGTTTGCCGGCTTTTTTTTCTGGCATAAGGAAATCCGCCCATGAAAACCTTACTGCTGAGCACGTCA
AAGCACTTTTTAATTGCCGT

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKGQILREMRVLKAITPDFEVQRRVAFIKAKLREAHSKTLVLGISGGVDSSVAGRLCQLAVNELNQETDSQQYRFIAVRL
PYLVQKDEHEAQMACEFIQPSKLVTVNVGSGSDGIHTETLTGFKAAGLELPEASKVDFVKGNVKARMRMIAQYEIAGLTG
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQIARYLGAPDVLVVKAPTADLECHRPGLEDEVALGLTYDQI
DDFLEGKEVPRSAEDKLIGIYKATQHKRQPIPTIYD

Sequences:

>Translated_276_residues
MKGQILREMRVLKAITPDFEVQRRVAFIKAKLREAHSKTLVLGISGGVDSSVAGRLCQLAVNELNQETDSQQYRFIAVRL
PYLVQKDEHEAQMACEFIQPSKLVTVNVGSGSDGIHTETLTGFKAAGLELPEASKVDFVKGNVKARMRMIAQYEIAGLTG
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQIARYLGAPDVLVVKAPTADLECHRPGLEDEVALGLTYDQI
DDFLEGKEVPRSAEDKLIGIYKATQHKRQPIPTIYD
>Mature_276_residues
MKGQILREMRVLKAITPDFEVQRRVAFIKAKLREAHSKTLVLGISGGVDSSVAGRLCQLAVNELNQETDSQQYRFIAVRL
PYLVQKDEHEAQMACEFIQPSKLVTVNVGSGSDGIHTETLTGFKAAGLELPEASKVDFVKGNVKARMRMIAQYEIAGLTG
GLVVGTDHSAENITGFYTKWGDGACDLAPLFGLNKRQVRQIARYLGAPDVLVVKAPTADLECHRPGLEDEVALGLTYDQI
DDFLEGKEVPRSAEDKLIGIYKATQHKRQPIPTIYD

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=277, Percent_Identity=50.9025270758123, Blast_Score=268, Evalue=3e-73,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_SHEAM (A1S5V3)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927429.1
- ProteinModelPortal:   A1S5V3
- SMR:   A1S5V3
- STRING:   A1S5V3
- GeneID:   4603804
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1552
- NMPDR:   fig|326297.7.peg.1467
- eggNOG:   COG0171
- HOGENOM:   HBG351567
- OMA:   KVAQATI
- PhylomeDB:   A1S5V3
- ProtClustDB:   PRK00768
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30383; Mature: 30383

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: ACT_SITE 45-45

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGQILREMRVLKAITPDFEVQRRVAFIKAKLREAHSKTLVLGISGGVDSSVAGRLCQLA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
VNELNQETDSQQYRFIAVRLPYLVQKDEHEAQMACEFIQPSKLVTVNVGSGSDGIHTETL
HHHHCCCCCCCCEEEEEEECCHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHH
TGFKAAGLELPEASKVDFVKGNVKARMRMIAQYEIAGLTGGLVVGTDHSAENITGFYTKW
CCHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEEC
GDGACDLAPLFGLNKRQVRQIARYLGAPDVLVVKAPTADLECHRPGLEDEVALGLTYDQI
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEECCHHHH
DDFLEGKEVPRSAEDKLIGIYKATQHKRQPIPTIYD
HHHHCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCC
>Mature Secondary Structure
MKGQILREMRVLKAITPDFEVQRRVAFIKAKLREAHSKTLVLGISGGVDSSVAGRLCQLA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHH
VNELNQETDSQQYRFIAVRLPYLVQKDEHEAQMACEFIQPSKLVTVNVGSGSDGIHTETL
HHHHCCCCCCCCEEEEEEECCHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHH
TGFKAAGLELPEASKVDFVKGNVKARMRMIAQYEIAGLTGGLVVGTDHSAENITGFYTKW
CCHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEEEEEC
GDGACDLAPLFGLNKRQVRQIARYLGAPDVLVVKAPTADLECHRPGLEDEVALGLTYDQI
CCCCCCHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEECCHHHH
DDFLEGKEVPRSAEDKLIGIYKATQHKRQPIPTIYD
HHHHCCCCCCCCCCCCEEEEEEHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA