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The map label for this gene is ligA

Identifier: 119774653

GI number: 119774653

Start: 1844244

End: 1846289

Strand: Direct

Name: ligA

Synonym: Sama_1516

Alternate gene names: 119774653

Gene position: 1844244-1846289 (Clockwise)

Preceding gene: 119774652

Following gene: 119774654

Centisome position: 42.83

GC content: 58.06

Gene sequence:

>2046_bases
ATGAGCGGCCCCGCGCATTTCCAAGGTACCATCATGCAAGTCAAAGCCGAAATTCAACGTCTTACCGCTGAAATCAATCG
TCACAACATCGCCTACTATGTGCAGGATGCGCCAAGCATTCCCGATGCGGAATACGACCGGCTGATGAACCAGCTCAAAG
CGCTGGAAGCCGAGTACCCACAGTTTGCCGAGCCCGACTCGCCCACCCAGCGTGTGGGCGGCGCAGCACTGAATAAATTT
GAACAGGTCAGCCACTTGAAACCCATGCTGAGCCTGGATAATGCCTTTAATGAAGAAGACTTCGTCGCCTTCGATAAGCG
CCTGACCGATAAGGTGGGCTCCCAGGTGTACTGCGCCGAGCCTAAACTCGACGGCCTCGCGGTGAGCCTGATTTACCGTA
ACGGTGTGCTTGAGCGTGCCGCTACCCGTGGAGATGGCGCCGTCGGGGAAGATATCAGCGTCAATATGCGCACCATCAAG
TCGGTACCACTGAGACTGCAGGGCGATGATATTCCGGCATTGGTGGAGGTGCGGGGAGAGGTCTTTATGCCAAGGGCCGC
CTTTGAAGCGCTGAATGACCGCGCCCGTGCAAAGGGTGACAAGCTCTTCGTCAATCCCCGTAACGCCGCCGCGGGCAGCT
TGCGTCAGCTCGACAGCAAGATAACCGCCGAGCGCTCACTGGCATTTTATGCCTATGCGCTGGGTGTGGTGCAGGACGAC
AATGGTGCCGATTTGGTGCTGGCGCCCACCCACAAAGGCCAACTCGAGCGCCTTACAGCCTTCGGTTTGCCGGTGAGCAA
GGAAGTGAAGCTCTGTGAAGGCCTTGCAGAGGTGATGGCCTACTATGCGGATATCCTTAAACGCCGTGACGAGCTTGCCT
TTGAAATCGATGGCGTGGTGATTAAGGTGAACGACATCGAAGCCCAGCAGCAGCTCGGGTTTGTGGCCCGTGCGCCGCGC
TGGGCTATCGCCTTTAAGTTTCCTGCACAGGAAGAGATGACTGTGCTCGAAGGCGTTGATTTTCAGGTGGGTCGTACCGG
CGCCGTGACCCCTGTAGCCCGCTTAAAACCTGTGTTTGTGGGCGGTGTGACCGTATCCAATGCGACTTTGCACAACGGTG
ATGAAATTGCCCGCCTTGGGGTGAAGATTGGCGATACCGTGATTATTCGCCGCGCCGGTGATGTGATCCCGCAAATTGTG
GCTGTGGTGCCTGAGCGCCGTCCTGATGAGGCGCAGGATATCCTGTTCCCGGACAATTGCCCTGTCTGCGGCTCGCTGGT
GGAGCGCATTGAAGGCGAGGCGGTGGCCCGCTGCAGCGGCGGGCTTTTCTGTGAAGCCCAGCGCCGGGAGGCCATCAAGC
ACTTTGCATCCCGAAAGGCCATGTATATCGATGGCATGGGCGACAAGGTGGTTGAGCAGCTTATTGATAAAGAGCTGGTG
GAAAGCCCGGCGGATCTCTTTAAGCTCAATGTGTCAAAGCTGACCATGCTTGAGCGCATGGGCACCAAGTCGGCCACCAA
TCTGGTGGCGGCCATTGAAGAAGCCAAAACCACCACCTTACCCAAGTTCCTCTATGCACTGGGTATTCGGGAAGTAGGGG
AGGCCACGGCCGCCAACCTGGCGAAGCACTTTCGAAGCCTCGAAGCCATCAGGGACGCCGATGTGGACGCCCTGATACAG
GTAGAAGATGTGGGTACAGTGGTTGCTCAGCACGTGGCGCACTTTTTTGCCCAGCCCCATAACCTTGAGGTGATAGATGC
GCTGATCGCGGCCGGGGTAAACTGGCCTGCCATCGAAAAGCCATCCGCCGATGCACAGCCGCTGCTGGGCCAAACCTGGG
TGCTCACGGGGACGCTCACCAGTCTTGGCCGCACCGAGGCCAAGGCCAAGCTTGAGGCCCTTGGTGCCAAGGTGGCTGGC
AGTGTGTCCAAGAATACCCACTGCGTAGTTGCCGGTGAAGCGGCGGGCTCCAAGCTTGCCAAGGCCCAGGAGTTGGGTAT
CCCGGTATTGGATGAAGCTGGTCTGATTGAGCTGATAGGCCTCTGA

Upstream 100 bases:

>100_bases
ACGGTCAGCGCCAACTCTGGAGCGATGCCACCAAGGCACAGTATCTCGCCCGCATCCGCGCTAATGCATAACAAGTTATT
GATTTACAAAGTGGGGCCGC

Downstream 100 bases:

>100_bases
TCTGATTTAAAAATTTATCCAGTTGAAAAAACGTCAACTTCAGGTTGGCGTTTTTTTGTTGCTCGCACTCGTGAAATAAA
ATATACAATATCCAGTAACA

Product: DNA ligase (NAD(+))

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 681; Mature: 680

Protein sequence:

>681_residues
MSGPAHFQGTIMQVKAEIQRLTAEINRHNIAYYVQDAPSIPDAEYDRLMNQLKALEAEYPQFAEPDSPTQRVGGAALNKF
EQVSHLKPMLSLDNAFNEEDFVAFDKRLTDKVGSQVYCAEPKLDGLAVSLIYRNGVLERAATRGDGAVGEDISVNMRTIK
SVPLRLQGDDIPALVEVRGEVFMPRAAFEALNDRARAKGDKLFVNPRNAAAGSLRQLDSKITAERSLAFYAYALGVVQDD
NGADLVLAPTHKGQLERLTAFGLPVSKEVKLCEGLAEVMAYYADILKRRDELAFEIDGVVIKVNDIEAQQQLGFVARAPR
WAIAFKFPAQEEMTVLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNGDEIARLGVKIGDTVIIRRAGDVIPQIV
AVVPERRPDEAQDILFPDNCPVCGSLVERIEGEAVARCSGGLFCEAQRREAIKHFASRKAMYIDGMGDKVVEQLIDKELV
ESPADLFKLNVSKLTMLERMGTKSATNLVAAIEEAKTTTLPKFLYALGIREVGEATAANLAKHFRSLEAIRDADVDALIQ
VEDVGTVVAQHVAHFFAQPHNLEVIDALIAAGVNWPAIEKPSADAQPLLGQTWVLTGTLTSLGRTEAKAKLEALGAKVAG
SVSKNTHCVVAGEAAGSKLAKAQELGIPVLDEAGLIELIGL

Sequences:

>Translated_681_residues
MSGPAHFQGTIMQVKAEIQRLTAEINRHNIAYYVQDAPSIPDAEYDRLMNQLKALEAEYPQFAEPDSPTQRVGGAALNKF
EQVSHLKPMLSLDNAFNEEDFVAFDKRLTDKVGSQVYCAEPKLDGLAVSLIYRNGVLERAATRGDGAVGEDISVNMRTIK
SVPLRLQGDDIPALVEVRGEVFMPRAAFEALNDRARAKGDKLFVNPRNAAAGSLRQLDSKITAERSLAFYAYALGVVQDD
NGADLVLAPTHKGQLERLTAFGLPVSKEVKLCEGLAEVMAYYADILKRRDELAFEIDGVVIKVNDIEAQQQLGFVARAPR
WAIAFKFPAQEEMTVLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNGDEIARLGVKIGDTVIIRRAGDVIPQIV
AVVPERRPDEAQDILFPDNCPVCGSLVERIEGEAVARCSGGLFCEAQRREAIKHFASRKAMYIDGMGDKVVEQLIDKELV
ESPADLFKLNVSKLTMLERMGTKSATNLVAAIEEAKTTTLPKFLYALGIREVGEATAANLAKHFRSLEAIRDADVDALIQ
VEDVGTVVAQHVAHFFAQPHNLEVIDALIAAGVNWPAIEKPSADAQPLLGQTWVLTGTLTSLGRTEAKAKLEALGAKVAG
SVSKNTHCVVAGEAAGSKLAKAQELGIPVLDEAGLIELIGL
>Mature_680_residues
SGPAHFQGTIMQVKAEIQRLTAEINRHNIAYYVQDAPSIPDAEYDRLMNQLKALEAEYPQFAEPDSPTQRVGGAALNKFE
QVSHLKPMLSLDNAFNEEDFVAFDKRLTDKVGSQVYCAEPKLDGLAVSLIYRNGVLERAATRGDGAVGEDISVNMRTIKS
VPLRLQGDDIPALVEVRGEVFMPRAAFEALNDRARAKGDKLFVNPRNAAAGSLRQLDSKITAERSLAFYAYALGVVQDDN
GADLVLAPTHKGQLERLTAFGLPVSKEVKLCEGLAEVMAYYADILKRRDELAFEIDGVVIKVNDIEAQQQLGFVARAPRW
AIAFKFPAQEEMTVLEGVDFQVGRTGAVTPVARLKPVFVGGVTVSNATLHNGDEIARLGVKIGDTVIIRRAGDVIPQIVA
VVPERRPDEAQDILFPDNCPVCGSLVERIEGEAVARCSGGLFCEAQRREAIKHFASRKAMYIDGMGDKVVEQLIDKELVE
SPADLFKLNVSKLTMLERMGTKSATNLVAAIEEAKTTTLPKFLYALGIREVGEATAANLAKHFRSLEAIRDADVDALIQV
EDVGTVVAQHVAHFFAQPHNLEVIDALIAAGVNWPAIEKPSADAQPLLGQTWVLTGTLTSLGRTEAKAKLEALGAKVAGS
VSKNTHCVVAGEAAGSKLAKAQELGIPVLDEAGLIELIGL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=673, Percent_Identity=59.7325408618128, Blast_Score=818, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=521, Percent_Identity=23.6084452975048, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_SHEAM (A1S5R7)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_927393.1
- ProteinModelPortal:   A1S5R7
- STRING:   A1S5R7
- GeneID:   4603768
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_1516
- NMPDR:   fig|326297.7.peg.1433
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   A1S5R7
- ProtClustDB:   CLSK906920
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 73672; Mature: 73541

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 122-122 BINDING 120-120 BINDING 143-143 BINDING 180-180 BINDING 302-302 BINDING 326-326

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPAHFQGTIMQVKAEIQRLTAEINRHNIAYYVQDAPSIPDAEYDRLMNQLKALEAEYP
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
QFAEPDSPTQRVGGAALNKFEQVSHLKPMLSLDNAFNEEDFVAFDKRLTDKVGSQVYCAE
CCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEEC
PKLDGLAVSLIYRNGVLERAATRGDGAVGEDISVNMRTIKSVPLRLQGDDIPALVEVRGE
CCCCCEEEEEEHHCCHHHHHHCCCCCCCCCCCEEEHHHHCCCCEEEECCCCCCCEEECCC
VFMPRAAFEALNDRARAKGDKLFVNPRNAAAGSLRQLDSKITAERSLAFYAYALGVVQDD
EECCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEECC
NGADLVLAPTHKGQLERLTAFGLPVSKEVKLCEGLAEVMAYYADILKRRDELAFEIDGVV
CCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEE
IKVNDIEAQQQLGFVARAPRWAIAFKFPAQEEMTVLEGVDFQVGRTGAVTPVARLKPVFV
EEEECCCHHHHHCHHHCCCCEEEEEECCCCCCHHHHCCCCEECCCCCCCCHHHHCCEEEE
GGVTVSNATLHNGDEIARLGVKIGDTVIIRRAGDVIPQIVAVVPERRPDEAQDILFPDNC
ECEEEECCEECCCCHHHHHCCEECCEEEEECHHHHHHHHHHHCCCCCCCCHHCCCCCCCC
PVCGSLVERIEGEAVARCSGGLFCEAQRREAIKHFASRKAMYIDGMGDKVVEQLIDKELV
CHHHHHHHHHCCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHH
ESPADLFKLNVSKLTMLERMGTKSATNLVAAIEEAKTTTLPKFLYALGIREVGEATAANL
HCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKHFRSLEAIRDADVDALIQVEDVGTVVAQHVAHFFAQPHNLEVIDALIAAGVNWPAIEK
HHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
PSADAQPLLGQTWVLTGTLTSLGRTEAKAKLEALGAKVAGSVSKNTHCVVAGEAAGSKLA
CCCCCCCCCCCEEEEEEHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHH
KAQELGIPVLDEAGLIELIGL
HHHHCCCCEECCCCCEEECCC
>Mature Secondary Structure 
SGPAHFQGTIMQVKAEIQRLTAEINRHNIAYYVQDAPSIPDAEYDRLMNQLKALEAEYP
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCC
QFAEPDSPTQRVGGAALNKFEQVSHLKPMLSLDNAFNEEDFVAFDKRLTDKVGSQVYCAE
CCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCEEEEEC
PKLDGLAVSLIYRNGVLERAATRGDGAVGEDISVNMRTIKSVPLRLQGDDIPALVEVRGE
CCCCCEEEEEEHHCCHHHHHHCCCCCCCCCCCEEEHHHHCCCCEEEECCCCCCCEEECCC
VFMPRAAFEALNDRARAKGDKLFVNPRNAAAGSLRQLDSKITAERSLAFYAYALGVVQDD
EECCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEECC
NGADLVLAPTHKGQLERLTAFGLPVSKEVKLCEGLAEVMAYYADILKRRDELAFEIDGVV
CCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEE
IKVNDIEAQQQLGFVARAPRWAIAFKFPAQEEMTVLEGVDFQVGRTGAVTPVARLKPVFV
EEEECCCHHHHHCHHHCCCCEEEEEECCCCCCHHHHCCCCEECCCCCCCCHHHHCCEEEE
GGVTVSNATLHNGDEIARLGVKIGDTVIIRRAGDVIPQIVAVVPERRPDEAQDILFPDNC
ECEEEECCEECCCCHHHHHCCEECCEEEEECHHHHHHHHHHHCCCCCCCCHHCCCCCCCC
PVCGSLVERIEGEAVARCSGGLFCEAQRREAIKHFASRKAMYIDGMGDKVVEQLIDKELV
CHHHHHHHHHCCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHH
ESPADLFKLNVSKLTMLERMGTKSATNLVAAIEEAKTTTLPKFLYALGIREVGEATAANL
HCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKHFRSLEAIRDADVDALIQVEDVGTVVAQHVAHFFAQPHNLEVIDALIAAGVNWPAIEK
HHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
PSADAQPLLGQTWVLTGTLTSLGRTEAKAKLEALGAKVAGSVSKNTHCVVAGEAAGSKLA
CCCCCCCCCCCEEEEEEHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHH
KAQELGIPVLDEAGLIELIGL
HHHHCCCCEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA