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The map label for this gene is pleD [H]

Identifier: 119774648

GI number: 119774648

Start: 1834784

End: 1836034

Strand: Direct

Name: pleD [H]

Synonym: Sama_1511

Alternate gene names: 119774648

Gene position: 1834784-1836034 (Clockwise)

Preceding gene: 119774647

Following gene: 119774651

Centisome position: 42.61

GC content: 53.08

Gene sequence:

>1251_bases
GTGCGGATTCTGGTGGTAGAAGACAGCAGCGTGGCAGCACGCATCCTCAAACATATGCTGACCCATCAGCTCGAGTGCAG
CATTGATGTTGCGCCGGACTTGGCCAGCGCCGCGGCCCTGCTGGCCAAGCACGACTATTTTGTGGCGATATGCGATCTCA
ACCTGCCCGATGCTCCCGGCGGGGAGAGTGTCGAGCTGGTGCTTGGCAATCAGACGCCCTGTATCGTGCTGACCGGCAGC
CTGGATGCACAGCAGCGCCGCCGTTTACTGCAAATGGGCATAGTGGATTATGTCTACAAGGAAAACCGTTACAGTTACGA
GTACGTAGTCAAACTTATCGCCACCCTTGCTCGCAACCGCCACACCAAGGTGTTGGTGGCCGACGATTCGGCCCTCAGTC
GCAAATTTGTCCGTAATCTGTTGGAGCTGCATCTTTTTGAAGTGATCGAAGCCGAAAACGGCGCCAGGGCATTGGAGCTG
CTTGACGCCGTGTCCGGCATTCGCCTGCTTATTGCCGATTACAATATGCCGGGTATCGATGGATTTGAACTTATCTTAAG
AGCCCGTGAAAAATTCAGCCGTGAGGAAATGGCGATTATCGGTCTTTCAAACGACACTGACGAAACCCTCACGGCACGTT
TTATTAAAAACGGTGCCAATGACTTTTTGCAAAAGCCCTTTGTACACGAAGAGTTTCACTGCCGCGTGAGCAACACCCTT
GAATCCCTTGAGATGGTCAGGCAGCTCTGGGAGCAGGCCAATTTGGATTACCTTACCTCGGTGTATAACCGCCGTTTTTT
CTTCAATCGCTTCAGTCAGCAGGTGCCTGTACTGGGGCAGCAGGGAGCGAGTTTTTCGCTCGCTGTGCTGGATATCGATT
TTTTTAAAGACGTCAATGACAAGCATGGCCATGATGTCGGCGATTTTGTACTCACGGAGTTTGCTTCGCGCTTGAAACAG
TCGCTTGGGCAGCATTTTACCGTTGCCCGTTTTGGTGGCGAGGAGTTTGTTGTGGCCTTGAAAGGACTGGATGGCAAGCG
CGCTCTGGCGCTGATGGAAGGTTTTCGTGAACGCCTGGCGATGGAGCCATTTGTGTTTGGCGAACTCGAACTGAGATTGA
CCGCAAGCATTGGCATTGTAGCGCTCGGGCATGACAGCGAAGAGACTCTGGACAGCCTGATAAAACAAGCCGATGTGGCA
CTCTATCAGGCCAAAACAAACGGGCGTAATCGCTGCGTGCTGGCCGAGTAA

Upstream 100 bases:

>100_bases
GAGAAGGGCTTGGCAGTGGCGACCTGGGGCTTCTGTGCTCCTTTGGCGCCGGTTATTCCATCGGCAGCTTTATTTTGCGG
GCAGTGTAAGGAGTATCGGT

Downstream 100 bases:

>100_bases
ATCAATTTTGCAAGCGCCGCTGAGAACATGAAAGCGCAGCAGAGAACATGAAAGCGCAGCAGATAAACCATGCTGCGCTT
TGTCATACCCTTCTGGTAGT

Product: response regulator receiver protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MRILVVEDSSVAARILKHMLTHQLECSIDVAPDLASAAALLAKHDYFVAICDLNLPDAPGGESVELVLGNQTPCIVLTGS
LDAQQRRRLLQMGIVDYVYKENRYSYEYVVKLIATLARNRHTKVLVADDSALSRKFVRNLLELHLFEVIEAENGARALEL
LDAVSGIRLLIADYNMPGIDGFELILRAREKFSREEMAIIGLSNDTDETLTARFIKNGANDFLQKPFVHEEFHCRVSNTL
ESLEMVRQLWEQANLDYLTSVYNRRFFFNRFSQQVPVLGQQGASFSLAVLDIDFFKDVNDKHGHDVGDFVLTEFASRLKQ
SLGQHFTVARFGGEEFVVALKGLDGKRALALMEGFRERLAMEPFVFGELELRLTASIGIVALGHDSEETLDSLIKQADVA
LYQAKTNGRNRCVLAE

Sequences:

>Translated_416_residues
MRILVVEDSSVAARILKHMLTHQLECSIDVAPDLASAAALLAKHDYFVAICDLNLPDAPGGESVELVLGNQTPCIVLTGS
LDAQQRRRLLQMGIVDYVYKENRYSYEYVVKLIATLARNRHTKVLVADDSALSRKFVRNLLELHLFEVIEAENGARALEL
LDAVSGIRLLIADYNMPGIDGFELILRAREKFSREEMAIIGLSNDTDETLTARFIKNGANDFLQKPFVHEEFHCRVSNTL
ESLEMVRQLWEQANLDYLTSVYNRRFFFNRFSQQVPVLGQQGASFSLAVLDIDFFKDVNDKHGHDVGDFVLTEFASRLKQ
SLGQHFTVARFGGEEFVVALKGLDGKRALALMEGFRERLAMEPFVFGELELRLTASIGIVALGHDSEETLDSLIKQADVA
LYQAKTNGRNRCVLAE
>Mature_416_residues
MRILVVEDSSVAARILKHMLTHQLECSIDVAPDLASAAALLAKHDYFVAICDLNLPDAPGGESVELVLGNQTPCIVLTGS
LDAQQRRRLLQMGIVDYVYKENRYSYEYVVKLIATLARNRHTKVLVADDSALSRKFVRNLLELHLFEVIEAENGARALEL
LDAVSGIRLLIADYNMPGIDGFELILRAREKFSREEMAIIGLSNDTDETLTARFIKNGANDFLQKPFVHEEFHCRVSNTL
ESLEMVRQLWEQANLDYLTSVYNRRFFFNRFSQQVPVLGQQGASFSLAVLDIDFFKDVNDKHGHDVGDFVLTEFASRLKQ
SLGQHFTVARFGGEEFVVALKGLDGKRALALMEGFRERLAMEPFVFGELELRLTASIGIVALGHDSEETLDSLIKQADVA
LYQAKTNGRNRCVLAE

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=172, Percent_Identity=37.2093023255814, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=36.969696969697, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87081881, Length=187, Percent_Identity=35.8288770053476, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1786584, Length=177, Percent_Identity=33.8983050847458, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI145693134, Length=193, Percent_Identity=31.6062176165803, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1787816, Length=165, Percent_Identity=32.7272727272727, Blast_Score=88, Evalue=9e-19,
Organism=Escherichia coli, GI87082007, Length=166, Percent_Identity=36.144578313253, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788381, Length=181, Percent_Identity=29.8342541436464, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788085, Length=183, Percent_Identity=29.5081967213115, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=166, Percent_Identity=30.1204819277108, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081974, Length=158, Percent_Identity=31.0126582278481, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787541, Length=165, Percent_Identity=30.9090909090909, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787056, Length=164, Percent_Identity=31.7073170731707, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788191, Length=128, Percent_Identity=32.03125, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 46717; Mature: 46717

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF ; PS00214 FABP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVVEDSSVAARILKHMLTHQLECSIDVAPDLASAAALLAKHDYFVAICDLNLPDAPG
CEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCCC
GESVELVLGNQTPCIVLTGSLDAQQRRRLLQMGIVDYVYKENRYSYEYVVKLIATLARNR
CCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
HTKVLVADDSALSRKFVRNLLELHLFEVIEAENGARALELLDAVSGIRLLIADYNMPGID
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCC
GFELILRAREKFSREEMAIIGLSNDTDETLTARFIKNGANDFLQKPFVHEEFHCRVSNTL
HHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHH
ESLEMVRQLWEQANLDYLTSVYNRRFFFNRFSQQVPVLGQQGASFSLAVLDIDFFKDVND
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEHHHHCCCC
KHGHDVGDFVLTEFASRLKQSLGQHFTVARFGGEEFVVALKGLDGKRALALMEGFRERLA
CCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHC
MEPFVFGELELRLTASIGIVALGHDSEETLDSLIKQADVALYQAKTNGRNRCVLAE
CCCEEEEEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHEEEECCCCCCEEEECC
>Mature Secondary Structure
MRILVVEDSSVAARILKHMLTHQLECSIDVAPDLASAAALLAKHDYFVAICDLNLPDAPG
CEEEEECCCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCCC
GESVELVLGNQTPCIVLTGSLDAQQRRRLLQMGIVDYVYKENRYSYEYVVKLIATLARNR
CCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
HTKVLVADDSALSRKFVRNLLELHLFEVIEAENGARALELLDAVSGIRLLIADYNMPGID
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCCCCC
GFELILRAREKFSREEMAIIGLSNDTDETLTARFIKNGANDFLQKPFVHEEFHCRVSNTL
HHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHH
ESLEMVRQLWEQANLDYLTSVYNRRFFFNRFSQQVPVLGQQGASFSLAVLDIDFFKDVND
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEHHHHCCCC
KHGHDVGDFVLTEFASRLKQSLGQHFTVARFGGEEFVVALKGLDGKRALALMEGFRERLA
CCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHC
MEPFVFGELELRLTASIGIVALGHDSEETLDSLIKQADVALYQAKTNGRNRCVLAE
CCCEEEEEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHEEEECCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA