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The map label for this gene is purR [H]

Identifier: 119774074

GI number: 119774074

Start: 1130902

End: 1131915

Strand: Reverse

Name: purR [H]

Synonym: Sama_0937

Alternate gene names: 119774074

Gene position: 1131915-1130902 (Counterclockwise)

Preceding gene: 119774077

Following gene: 119774071

Centisome position: 26.29

GC content: 50.59

Gene sequence:

>1014_bases
ATGAAAGTCACAATTAATGACGTTGCCAAATACGCCGGCGTTTCTATCAAGACGGTTTCCCGGGTTACCAACAACGAACC
CTCGGTGAAACAGGCAACCATAGACAAGGTAAATGAAGCCATCAAGGCGCTTAATTACCAGCCCAACCTGGCTGCGCGTA
ACCTCGCCGGCACCAAGTCTTATGTTATCGGCTTTATCTACGACAATCCCAATGCCTACTATATTATCGATATGCAAAAC
GGCATTTTATCGGCATGTCGCGATAAGGGTTATGAACTCCTGATCCACCCTTGCGATGCTACCGCCGACAGTATTTGCGA
AGAGTTAACTGCCTTGGTAAAGCACGCCAGACTGGCCGGATTGGTCCTGACTCCTCCCCTGTCGGAAGATCCCAAAATTT
TAGCGGCACTCGACAGCATTGAGGCCCATTACGTGCGCATCATTGCCGGAGACAAAACCAAAGAAAGTTGTGGTTTGACG
GTATTGGTTAATGACAGGCACGGCGCGGTGTCTATTACCCAACATCTGATTGATTTGGGGCACAAACATATCGCTTTTAT
CAGCGGGGACGAGCAGCACGAATCGACCAAAGAGCGCTTCGCCGGTTTTTGCGACGCTCTCTCACTCAACAACATTACCT
TGAACCCGGCGTTTGTAATTGAAGGGCAGTATTCGTTTGAATCAGGTGTTGAAGGGGCTAAACAGCTGCTTTCAATGACA
CCAAGGCCAACGGCCATCGTCGCGTGTAACGATGAAATAGCGGCTGGTGCCTTGTTTGCCGCGCGTTTGCAGGGGGTTGA
TATCCCTCGCGATATCTCAATTGTCGGCTTCGAAGACAGCCCGTTTTCACGCCAAACCTGGCCCAAACTGACTACGGTTC
ATCAACCGAATCAAAAAATAGCACAGGTCGCTACCGAGCTGCTGATTGCTAATCGTAAAGAGCAGCGCCTGGACAGTGCC
AAGGTGTTCACCCCCGAGCCTGTTGTCCGCGATTCCAGCAGCAACCCAGGTTAA

Upstream 100 bases:

>100_bases
TCAACCGATACAAGTGCAATATTTGGTATCCCAAACGCCAGTCATCTGTCAGTTCCCATCCAAATAGCGCCACTAATAGA
TTGAATTACTGAGTGTTTAG

Downstream 100 bases:

>100_bases
AAAAACGGGCTCAATGCAACTTAAGCTTGGGGCGCTGCCATCGCAGCAGCTTTTGCGCCAAGCGATACACCAGCGCCGAA
AACCAGCCAAATAGACTGGA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATIDKVNEAIKALNYQPNLAARNLAGTKSYVIGFIYDNPNAYYIIDMQN
GILSACRDKGYELLIHPCDATADSICEELTALVKHARLAGLVLTPPLSEDPKILAALDSIEAHYVRIIAGDKTKESCGLT
VLVNDRHGAVSITQHLIDLGHKHIAFISGDEQHESTKERFAGFCDALSLNNITLNPAFVIEGQYSFESGVEGAKQLLSMT
PRPTAIVACNDEIAAGALFAARLQGVDIPRDISIVGFEDSPFSRQTWPKLTTVHQPNQKIAQVATELLIANRKEQRLDSA
KVFTPEPVVRDSSSNPG

Sequences:

>Translated_337_residues
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATIDKVNEAIKALNYQPNLAARNLAGTKSYVIGFIYDNPNAYYIIDMQN
GILSACRDKGYELLIHPCDATADSICEELTALVKHARLAGLVLTPPLSEDPKILAALDSIEAHYVRIIAGDKTKESCGLT
VLVNDRHGAVSITQHLIDLGHKHIAFISGDEQHESTKERFAGFCDALSLNNITLNPAFVIEGQYSFESGVEGAKQLLSMT
PRPTAIVACNDEIAAGALFAARLQGVDIPRDISIVGFEDSPFSRQTWPKLTTVHQPNQKIAQVATELLIANRKEQRLDSA
KVFTPEPVVRDSSSNPG
>Mature_337_residues
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATIDKVNEAIKALNYQPNLAARNLAGTKSYVIGFIYDNPNAYYIIDMQN
GILSACRDKGYELLIHPCDATADSICEELTALVKHARLAGLVLTPPLSEDPKILAALDSIEAHYVRIIAGDKTKESCGLT
VLVNDRHGAVSITQHLIDLGHKHIAFISGDEQHESTKERFAGFCDALSLNNITLNPAFVIEGQYSFESGVEGAKQLLSMT
PRPTAIVACNDEIAAGALFAARLQGVDIPRDISIVGFEDSPFSRQTWPKLTTVHQPNQKIAQVATELLIANRKEQRLDSA
KVFTPEPVVRDSSSNPG

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=318, Percent_Identity=31.4465408805031, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789068, Length=340, Percent_Identity=30.5882352941176, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1789202, Length=336, Percent_Identity=31.547619047619, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790194, Length=338, Percent_Identity=29.585798816568, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=29.7619047619048, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1790369, Length=331, Percent_Identity=29.0030211480363, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1788474, Length=308, Percent_Identity=32.7922077922078, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1790715, Length=316, Percent_Identity=25.3164556962025, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1787580, Length=321, Percent_Identity=27.1028037383178, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI48994940, Length=308, Percent_Identity=26.2987012987013, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787906, Length=290, Percent_Identity=28.6206896551724, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789456, Length=335, Percent_Identity=27.4626865671642, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790689, Length=301, Percent_Identity=24.5847176079734, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36636; Mature: 36636

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATIDKVNEAIKALNYQPNLAARNLAGTKS
CEEEHHHHHHHHCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCE
YVIGFIYDNPNAYYIIDMQNGILSACRDKGYELLIHPCDATADSICEELTALVKHARLAG
EEEEEEEECCCEEEEEECCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC
LVLTPPLSEDPKILAALDSIEAHYVRIIAGDKTKESCGLTVLVNDRHGAVSITQHLIDLG
EEECCCCCCCCHHEEHHHCCCEEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCC
HKHIAFISGDEQHESTKERFAGFCDALSLNNITLNPAFVIEGQYSFESGVEGAKQLLSMT
CCEEEEEECCCHHHHHHHHHHHHHHHEECCCEEECCEEEEECCCCHHHHHHHHHHHHHCC
PRPTAIVACNDEIAAGALFAARLQGVDIPRDISIVGFEDSPFSRQTWPKLTTVHQPNQKI
CCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCHHH
AQVATELLIANRKEQRLDSAKVFTPEPVVRDSSSNPG
HHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVTINDVAKYAGVSIKTVSRVTNNEPSVKQATIDKVNEAIKALNYQPNLAARNLAGTKS
CEEEHHHHHHHHCCEEEEHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCE
YVIGFIYDNPNAYYIIDMQNGILSACRDKGYELLIHPCDATADSICEELTALVKHARLAG
EEEEEEEECCCEEEEEECCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCC
LVLTPPLSEDPKILAALDSIEAHYVRIIAGDKTKESCGLTVLVNDRHGAVSITQHLIDLG
EEECCCCCCCCHHEEHHHCCCEEEEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHCC
HKHIAFISGDEQHESTKERFAGFCDALSLNNITLNPAFVIEGQYSFESGVEGAKQLLSMT
CCEEEEEECCCHHHHHHHHHHHHHHHEECCCEEECCEEEEECCCCHHHHHHHHHHHHHCC
PRPTAIVACNDEIAAGALFAARLQGVDIPRDISIVGFEDSPFSRQTWPKLTTVHQPNQKI
CCCCEEEEECCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCHHH
AQVATELLIANRKEQRLDSAKVFTPEPVVRDSSSNPG
HHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA