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The map label for this gene is nadB [H]

Identifier: 119774010

GI number: 119774010

Start: 1062349

End: 1063956

Strand: Reverse

Name: nadB [H]

Synonym: Sama_0873

Alternate gene names: 119774010

Gene position: 1063956-1062349 (Counterclockwise)

Preceding gene: 119774023

Following gene: 119773999

Centisome position: 24.71

GC content: 58.08

Gene sequence:

>1608_bases
ATGAAACAAGCAGTTGAACACCAGTCAGACATTCTGGTCATCGGCAGCGGTGCTGCTGGCCTGACTTTGGCACTCCATCT
TGCTGAAAAAGCAAAGGTAATTCTGATTTCCAAAGGGCCGCTCAGTGAAGGGTCCACCCTGTACGCCCAGGGAGGCATTG
CCTCAGTGTTCGACGAGGGCGACACCATTGAGTCCCACGTCGAGGACACTCTGGTGGCCGGTGCGGGTATCTGTGATGAG
ACTGTGGTCACCTATACCGCCGCCAACGCCAAGGCAGCCATGCAGTGGCTCATCAGCTGCGGCGTGCCCTTTGATAAAGA
AGAGCACATCACCAACGAAGCCCAGCCTTACCACCTGACCCGGGAAGGTGGCCACAGCCACAGACGCATTCTGCATGCCG
CAGACGCCACCGGCAAAGCGGTTCAGACCACCCTGCAGGAGCTGGCAGCCAACCACCCTAATATTCAGGTGCTGGAGCGC
TACAACGCCATCGACCTTATTACCACCCGCAAACTGGGCCGCACCGGCAATCGGGTGCTGGGCGCCTATGTGTGGAATCG
CAGTCAGGAGCAGGTTGAGACCGTGAAGGCACGTTTCGTGGCGCTCGCCACCGGCGGCAGCTCCAAGGTGTATCAGTACA
CATCCAATCCGGACGTGGCCAGCGGCGATGGCATTGCCATGGCCTGGCGTGCCGGGTGCCGGGTGGCCAACATGGAGTTT
AACCAATTCCACCCAACCTGCCTGTACCATGCAGAAGCCCGTAACTTCCTGCTGACCGAAGCCCTGCGCGGCGAAGGCGC
GTATCTGCGCCGACCCGATGGCAGCCGCTTTATGCCGGACTTCGATGAGCGCGCCGAACTCGCGCCCCGCGATATTGTCG
CCCGCGCCATCGACTATGAGATGAAACGCCTGGGTGTGGACTGCATGTATCTGGACATCAGCCACAAACCGGCGGACTTT
ATTATCAAGCACTTCCCGACCATACACAGCCGTTGCCTGGAATTGGGTCTGGACATTACCAAAGAGCCCATCCCCATAGT
TCCCGCCGCCCACTATACCTGCGGCGGTGTGATGACAGATTTGCACGGTCAGACGGATCTCAACGGTCTTTATGCCATAG
GCGAAGTGGCCTATACGGGTCTTCATGGAGCCAACCGTCTGGCCAGCAACTCTCTGCTGGAATGTCTGGTGTTTGCCCGC
GCCGCCGCACAGGACATTGAGAGTTTGATGGGCAAGATTGCCATGCCGGGTCAGCTGCCAGCCTGGGATGAAAGCCGGGT
GAGTAACTCAGACGAAGAAGTGGTCATTGCCCACAACTGGCATGAACTGCGCCTCTTTATGTGGGACTACGTAGGCATAG
TTCGCACCGATAAACGCCTCGAGCGGGCGCTCAGGCGGGTAACCATGCTGCAACAGGAAATCCAGGAGTATTACTCCAAC
TTCCGGGTCAGCAATAACCTGCTGGAGCTGCGTAATCTGGTGCAGGTGGCCGAACTTATCATTCGCTGTGCCATGGCGCG
TAAAGAAAGCCGCGGCCTGCACTGTAACCTCGACCATCCTGAAAAACTCGACGTCTCGGGCCCTACGATTTTAACGCCCG
AGCGTTAA

Upstream 100 bases:

>100_bases
GTTCTGCGATTTTTATTAAAAAAACCCCGACCGGGGCAAGTACCCTAAATTCCCAACAAGGTTTATCATAGCGGGACATT
TGAATCCGTCATGATACCCG

Downstream 100 bases:

>100_bases
TGAACAGCAGCAAGCGGCACAAATCCCGATAAGGCCCGTCATCGAGCATATCGGCAAATACAAATCGAAGGCGGCCCTGG
CCGCTTTCTTTTTTTATCAG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MKQAVEHQSDILVIGSGAAGLTLALHLAEKAKVILISKGPLSEGSTLYAQGGIASVFDEGDTIESHVEDTLVAGAGICDE
TVVTYTAANAKAAMQWLISCGVPFDKEEHITNEAQPYHLTREGGHSHRRILHAADATGKAVQTTLQELAANHPNIQVLER
YNAIDLITTRKLGRTGNRVLGAYVWNRSQEQVETVKARFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANMEF
NQFHPTCLYHAEARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAIDYEMKRLGVDCMYLDISHKPADF
IIKHFPTIHSRCLELGLDITKEPIPIVPAAHYTCGGVMTDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFAR
AAAQDIESLMGKIAMPGQLPAWDESRVSNSDEEVVIAHNWHELRLFMWDYVGIVRTDKRLERALRRVTMLQQEIQEYYSN
FRVSNNLLELRNLVQVAELIIRCAMARKESRGLHCNLDHPEKLDVSGPTILTPER

Sequences:

>Translated_535_residues
MKQAVEHQSDILVIGSGAAGLTLALHLAEKAKVILISKGPLSEGSTLYAQGGIASVFDEGDTIESHVEDTLVAGAGICDE
TVVTYTAANAKAAMQWLISCGVPFDKEEHITNEAQPYHLTREGGHSHRRILHAADATGKAVQTTLQELAANHPNIQVLER
YNAIDLITTRKLGRTGNRVLGAYVWNRSQEQVETVKARFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANMEF
NQFHPTCLYHAEARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAIDYEMKRLGVDCMYLDISHKPADF
IIKHFPTIHSRCLELGLDITKEPIPIVPAAHYTCGGVMTDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFAR
AAAQDIESLMGKIAMPGQLPAWDESRVSNSDEEVVIAHNWHELRLFMWDYVGIVRTDKRLERALRRVTMLQQEIQEYYSN
FRVSNNLLELRNLVQVAELIIRCAMARKESRGLHCNLDHPEKLDVSGPTILTPER
>Mature_535_residues
MKQAVEHQSDILVIGSGAAGLTLALHLAEKAKVILISKGPLSEGSTLYAQGGIASVFDEGDTIESHVEDTLVAGAGICDE
TVVTYTAANAKAAMQWLISCGVPFDKEEHITNEAQPYHLTREGGHSHRRILHAADATGKAVQTTLQELAANHPNIQVLER
YNAIDLITTRKLGRTGNRVLGAYVWNRSQEQVETVKARFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANMEF
NQFHPTCLYHAEARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAIDYEMKRLGVDCMYLDISHKPADF
IIKHFPTIHSRCLELGLDITKEPIPIVPAAHYTCGGVMTDLHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFAR
AAAQDIESLMGKIAMPGQLPAWDESRVSNSDEEVVIAHNWHELRLFMWDYVGIVRTDKRLERALRRVTMLQQEIQEYYSN
FRVSNNLLELRNLVQVAELIIRCAMARKESRGLHCNLDHPEKLDVSGPTILTPER

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=515, Percent_Identity=32.4271844660194, Blast_Score=199, Evalue=7e-51,
Organism=Escherichia coli, GI1788928, Length=535, Percent_Identity=73.0841121495327, Blast_Score=816, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=538, Percent_Identity=31.9702602230483, Blast_Score=235, Evalue=6e-63,
Organism=Escherichia coli, GI1786942, Length=563, Percent_Identity=32.3268206039076, Blast_Score=196, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17550100, Length=567, Percent_Identity=32.2751322751323, Blast_Score=204, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI17505833, Length=536, Percent_Identity=30.5970149253731, Blast_Score=201, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI71986328, Length=471, Percent_Identity=27.8131634819533, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322701, Length=558, Percent_Identity=32.078853046595, Blast_Score=219, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6322416, Length=568, Percent_Identity=30.4577464788732, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6322511, Length=492, Percent_Identity=25.4065040650407, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320788, Length=489, Percent_Identity=26.3803680981595, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI17137288, Length=573, Percent_Identity=30.5410122164049, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24655642, Length=573, Percent_Identity=30.5410122164049, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24655647, Length=573, Percent_Identity=30.5410122164049, Blast_Score=199, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24663005, Length=562, Percent_Identity=30.7829181494662, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59237; Mature: 59237

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQAVEHQSDILVIGSGAAGLTLALHLAEKAKVILISKGPLSEGSTLYAQGGIASVFDEG
CCCCCCCCCCEEEEECCCCCHHHEEEECCCCEEEEEECCCCCCCCEEEECCCCHHHHCCC
DTIESHVEDTLVAGAGICDETVVTYTAANAKAAMQWLISCGVPFDKEEHITNEAQPYHLT
CHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEE
REGGHSHRRILHAADATGKAVQTTLQELAANHPNIQVLERYNAIDLITTRKLGRTGNRVL
CCCCCCHHHEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEEHHHCCCCCCEEE
GAYVWNRSQEQVETVKARFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANMEF
EEEECCCCHHHHHHHHHHEEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCEEECCCC
NQFHPTCLYHAEARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAIDYE
CCCCCEEEEECHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHH
MKRLGVDCMYLDISHKPADFIIKHFPTIHSRCLELGLDITKEPIPIVPAAHYTCGGVMTD
HHHCCCEEEEEEECCCCHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCC
LHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFARAAAQDIESLMGKIAMPGQLP
CCCCCCCCHHEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AWDESRVSNSDEEVVIAHNWHELRLFMWDYVGIVRTDKRLERALRRVTMLQQEIQEYYSN
CCCHHHCCCCCCCEEEEECCHHEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
FRVSNNLLELRNLVQVAELIIRCAMARKESRGLHCNLDHPEKLDVSGPTILTPER
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEECCCCCEEECCCC
>Mature Secondary Structure
MKQAVEHQSDILVIGSGAAGLTLALHLAEKAKVILISKGPLSEGSTLYAQGGIASVFDEG
CCCCCCCCCCEEEEECCCCCHHHEEEECCCCEEEEEECCCCCCCCEEEECCCCHHHHCCC
DTIESHVEDTLVAGAGICDETVVTYTAANAKAAMQWLISCGVPFDKEEHITNEAQPYHLT
CHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCHHHCCCCCCCEEEE
REGGHSHRRILHAADATGKAVQTTLQELAANHPNIQVLERYNAIDLITTRKLGRTGNRVL
CCCCCCHHHEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCEEEEEHHHCCCCCCEEE
GAYVWNRSQEQVETVKARFVALATGGSSKVYQYTSNPDVASGDGIAMAWRAGCRVANMEF
EEEECCCCHHHHHHHHHHEEEEEECCCCCEEEECCCCCCCCCCCEEEEECCCCEEECCCC
NQFHPTCLYHAEARNFLLTEALRGEGAYLRRPDGSRFMPDFDERAELAPRDIVARAIDYE
CCCCCEEEEECHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHH
MKRLGVDCMYLDISHKPADFIIKHFPTIHSRCLELGLDITKEPIPIVPAAHYTCGGVMTD
HHHCCCEEEEEEECCCCHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHCC
LHGQTDLNGLYAIGEVAYTGLHGANRLASNSLLECLVFARAAAQDIESLMGKIAMPGQLP
CCCCCCCCHHEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AWDESRVSNSDEEVVIAHNWHELRLFMWDYVGIVRTDKRLERALRRVTMLQQEIQEYYSN
CCCHHHCCCCCCCEEEEECCHHEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
FRVSNNLLELRNLVQVAELIIRCAMARKESRGLHCNLDHPEKLDVSGPTILTPER
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCEECCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]