Click here to switch to the map view.

The map label for this gene is hemL

Identifier: 119773976

GI number: 119773976

Start: 1023262

End: 1024545

Strand: Reverse

Name: hemL

Synonym: Sama_0839

Alternate gene names: 119773976

Gene position: 1024545-1023262 (Counterclockwise)

Preceding gene: 119773977

Following gene: 119773974

Centisome position: 23.79

GC content: 56.62

Gene sequence:

>1284_bases
ATGACCCGTTCCGAAACCTTGTTTGAACAGGCTAAAAAAACCATTCCCGGTGGCGTTAACTCACCGGTGCGCGCCTTCAA
TGGCGTGGGCGGTACCCCTCGTTTTATAGAAAAGGCCGATGGCGCATATATCTATGACGCCGACGGCAAGGCCTATATCG
ATTATGTGGGCTCCTGGGGCCCAATGATTCTTGGCCACAACCACCCATCCATTCGCGAAGCCGTGCTTAAAGCCGTTCAC
AATGGTTTGTCTTTCGGTGCCCCCACCGAGCTTGAAGTCATCATGGCCGAGAAGGTAATCGAAATGGTGCCTTCCATGGA
TCAGGTGCGTATGGTGAGTTCAGGCACCGAAGCCACCATGAGCGCCATCCGTCTGGCCCGTGGTTACACCAAGCGCGACA
AAATCCTTAAGTTTGAAGGTTGCTACCATGGCCACGCCGACTGTCTGCTGGTAAAAGCCGGCTCTGGCGCCCTGACTCTG
GGTCAGCCAAGCTCACCCGGTATCCCGGAAGATTTCGCCAAGCACACTCTCACTGCCGTGTATAACGACCTTGAGTCTGT
ACAGTCTTTCTTCGACCAGTATCCTGAAGACATCGCCTGTATCATCATCGAGCCGGTTGCCGGCAACATGAACTGTATTC
CGCCGGTTCCCGGATTTCTGGAAGGCCTGCGCGCCCTCTGTGACAAGTACGGTGCGCTCTTTATCATCGATGAAGTAATG
ACGGGCTTCCGTGTATCCAGAAGCGGTGCTCAGGGCCACTATGGCGTGACCCCGGACCTCACCACCCTGGGTAAGGTGAT
TGGCGGCGGCATGCCCGTTGGCGCCTTTGGCGGCAAAAAAGACGTGATGCAGTATCTGGCTCCTGCCGGCCCTGTGTATC
AGGCAGGTACTCTTTCGGGTAATCCCATTGCCATGACAGCCGGTCTGGCGCAGCTCGACGCCCTTTGTGCCGATGGCCTG
TATGAAGAACTTGCCGCCAAGACCAAGCGCATCGCCGAAGGCTTCAAGGCCGCTGCCGACAAGCATGGTATCCCGATGGC
CATCAACTATGTTGGCGGCATGTTCGGCTTCTTCTTCACCGATGAGCAGCATATCACCCGTTTCGACCAGGTGACCCGCT
GTAACATGGACCACTTCCGCGCCTTCTACCACGGCATGCTGGACGAGGGTGTGTATCTGGCCCCAAGCGCTTATGAGGCT
GGCTTCCTGTCCATGGCCCACGGTGACAAGGAAATCGAAGAAACCCTGGCCGCCGCTGATCGCGTACTGGCCCGCATGAA
GTAA

Upstream 100 bases:

>100_bases
TGGGCGTGGACTCACAGCTGGAAAAGTACGAGTGTCCGGTTAACTGGTATTCACGCAAGGCTGACTTCTAAGGTCGGCTA
CTGACTTAAAGGATTGATAA

Downstream 100 bases:

>100_bases
CTGCAACGCCCGGATGCTCGCCGTTATCGCGGCACTGCAAAACGAAAGGACGCTATCGGCGTCCTTTTTCATTAGATTCG
AATCACAGCAAACCAGACAG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTRSETLFEQAKKTIPGGVNSPVRAFNGVGGTPRFIEKADGAYIYDADGKAYIDYVGSWGPMILGHNHPSIREAVLKAVH
NGLSFGAPTELEVIMAEKVIEMVPSMDQVRMVSSGTEATMSAIRLARGYTKRDKILKFEGCYHGHADCLLVKAGSGALTL
GQPSSPGIPEDFAKHTLTAVYNDLESVQSFFDQYPEDIACIIIEPVAGNMNCIPPVPGFLEGLRALCDKYGALFIIDEVM
TGFRVSRSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGKKDVMQYLAPAGPVYQAGTLSGNPIAMTAGLAQLDALCADGL
YEELAAKTKRIAEGFKAAADKHGIPMAINYVGGMFGFFFTDEQHITRFDQVTRCNMDHFRAFYHGMLDEGVYLAPSAYEA
GFLSMAHGDKEIEETLAAADRVLARMK

Sequences:

>Translated_427_residues
MTRSETLFEQAKKTIPGGVNSPVRAFNGVGGTPRFIEKADGAYIYDADGKAYIDYVGSWGPMILGHNHPSIREAVLKAVH
NGLSFGAPTELEVIMAEKVIEMVPSMDQVRMVSSGTEATMSAIRLARGYTKRDKILKFEGCYHGHADCLLVKAGSGALTL
GQPSSPGIPEDFAKHTLTAVYNDLESVQSFFDQYPEDIACIIIEPVAGNMNCIPPVPGFLEGLRALCDKYGALFIIDEVM
TGFRVSRSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGKKDVMQYLAPAGPVYQAGTLSGNPIAMTAGLAQLDALCADGL
YEELAAKTKRIAEGFKAAADKHGIPMAINYVGGMFGFFFTDEQHITRFDQVTRCNMDHFRAFYHGMLDEGVYLAPSAYEA
GFLSMAHGDKEIEETLAAADRVLARMK
>Mature_426_residues
TRSETLFEQAKKTIPGGVNSPVRAFNGVGGTPRFIEKADGAYIYDADGKAYIDYVGSWGPMILGHNHPSIREAVLKAVHN
GLSFGAPTELEVIMAEKVIEMVPSMDQVRMVSSGTEATMSAIRLARGYTKRDKILKFEGCYHGHADCLLVKAGSGALTLG
QPSSPGIPEDFAKHTLTAVYNDLESVQSFFDQYPEDIACIIIEPVAGNMNCIPPVPGFLEGLRALCDKYGALFIIDEVMT
GFRVSRSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGKKDVMQYLAPAGPVYQAGTLSGNPIAMTAGLAQLDALCADGLY
EELAAKTKRIAEGFKAAADKHGIPMAINYVGGMFGFFFTDEQHITRFDQVTRCNMDHFRAFYHGMLDEGVYLAPSAYEAG
FLSMAHGDKEIEETLAAADRVLARMK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=303, Percent_Identity=29.7029702970297, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI284507298, Length=221, Percent_Identity=31.2217194570136, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=69.9530516431925, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=390, Percent_Identity=31.025641025641, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1788044, Length=333, Percent_Identity=31.2312312312312, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI145693181, Length=350, Percent_Identity=29.1428571428571, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789759, Length=336, Percent_Identity=29.7619047619048, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787560, Length=359, Percent_Identity=29.2479108635098, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1786991, Length=426, Percent_Identity=25.8215962441315, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25144271, Length=303, Percent_Identity=27.7227722772277, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71992977, Length=433, Percent_Identity=26.0969976905312, Blast_Score=114, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI25144274, Length=150, Percent_Identity=35.3333333333333, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323470, Length=343, Percent_Identity=28.8629737609329, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=352, Percent_Identity=25, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357415, Length=298, Percent_Identity=27.1812080536913, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21356575, Length=339, Percent_Identity=24.188790560472, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_SHEAM (A1S3U0)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926716.1
- ProteinModelPortal:   A1S3U0
- SMR:   A1S3U0
- STRING:   A1S3U0
- GeneID:   4603091
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0839
- NMPDR:   fig|326297.7.peg.790
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   TANQFES
- PhylomeDB:   A1S3U0
- ProtClustDB:   PRK00062
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45922; Mature: 45791

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSETLFEQAKKTIPGGVNSPVRAFNGVGGTPRFIEKADGAYIYDADGKAYIDYVGSWG
CCCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHCCCCCEEEECCCCEEEEEECCCC
PMILGHNHPSIREAVLKAVHNGLSFGAPTELEVIMAEKVIEMVPSMDQVRMVSSGTEATM
CEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SAIRLARGYTKRDKILKFEGCYHGHADCLLVKAGSGALTLGQPSSPGIPEDFAKHTLTAV
HHHHHHHCCCCHHCEEEECCCCCCCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHH
YNDLESVQSFFDQYPEDIACIIIEPVAGNMNCIPPVPGFLEGLRALCDKYGALFIIDEVM
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHHH
TGFRVSRSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGKKDVMQYLAPAGPVYQAGTLSG
HHHEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMTAGLAQLDALCADGLYEELAAKTKRIAEGFKAAADKHGIPMAINYVGGMFGFFFT
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCC
DEQHITRFDQVTRCNMDHFRAFYHGMLDEGVYLAPSAYEAGFLSMAHGDKEIEETLAAAD
CHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHCCHHHHHHHHHHHH
RVLARMK
HHHHHCC
>Mature Secondary Structure 
TRSETLFEQAKKTIPGGVNSPVRAFNGVGGTPRFIEKADGAYIYDADGKAYIDYVGSWG
CCHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCHHHHCCCCCEEEECCCCEEEEEECCCC
PMILGHNHPSIREAVLKAVHNGLSFGAPTELEVIMAEKVIEMVPSMDQVRMVSSGTEATM
CEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
SAIRLARGYTKRDKILKFEGCYHGHADCLLVKAGSGALTLGQPSSPGIPEDFAKHTLTAV
HHHHHHHCCCCHHCEEEECCCCCCCCCEEEEECCCCEEECCCCCCCCCCHHHHHHHHHHH
YNDLESVQSFFDQYPEDIACIIIEPVAGNMNCIPPVPGFLEGLRALCDKYGALFIIDEVM
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCEEEHHHHH
TGFRVSRSGAQGHYGVTPDLTTLGKVIGGGMPVGAFGGKKDVMQYLAPAGPVYQAGTLSG
HHHEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPIAMTAGLAQLDALCADGLYEELAAKTKRIAEGFKAAADKHGIPMAINYVGGMFGFFFT
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCC
DEQHITRFDQVTRCNMDHFRAFYHGMLDEGVYLAPSAYEAGFLSMAHGDKEIEETLAAAD
CHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEECCCCCCCCHHHHHCCHHHHHHHHHHHH
RVLARMK
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA