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The map label for this gene is pyrB [H]

Identifier: 119773977

GI number: 119773977

Start: 1024575

End: 1025594

Strand: Reverse

Name: pyrB [H]

Synonym: Sama_0840

Alternate gene names: 119773977

Gene position: 1025594-1024575 (Counterclockwise)

Preceding gene: 119773978

Following gene: 119773976

Centisome position: 23.82

GC content: 54.71

Gene sequence:

>1020_bases
ATGACTCAGTTCAAAGGATCTCACATCCTCTCCGTAAACCAGTTCAATCTGGACTCCATTCACACTATTTTCAATGTTGC
CGAGCGTATGGCGCCCTATGCGTTACGGGAAAAACGTACCCGGGTGTTGGATGGTGCCATATTGGGGAATCTGTTCTTTG
AACCCAGCACCCGTACCCGGGTGAGCTTTGGCTGTGCCTTTAACCTGCTGGGCGGCCGCGTGGCGGAAACCGTGGGCATG
GCCTCATCTTCCTTGTCCAAGGGTGAATCGCTGTACGACACAGCCCGTGTGCTGTCGACATACTCAGATGTGATTGCCAT
GCGTCATCCCGATGCCTACTCGGTGCGCGAATTCGCTGAGGGCAGCCGGGTGCCGGTGATCAACGGTGGCGACGGCCCCA
ACGAACATCCGACTCAGGCCCTGCTGGATTTGTTCACCATCCAGAAGGAGCTCAGCCACGCCGGTATGAGTATTGACGGC
ATGCACATCGCCATGGTGGGCGACCTTAAATTTGGCCGCACAGTACACTCACTGTCACGCCTGCTGTGTCTGTACAAAAA
CGTCAGCTTTACCCTGATTTCTCCCAAAGAGCTGGCAATGCCCGATTATGTGATAGCCGACATTGAAAATGCCGGCCATA
AGGTCACAATAACCGATCAGCTGGAAGGCAATCTGGACAAGGCCGACATCCTGTACCTGACCCGAATTCAGGAAGAGCGC
TTCCCGTCACAGGATGAAGCCAACAAGTATCGCGGCAAGTTCCGTCTCAACCGCAGCATTTACACCCAGCACTGTAAGTC
CAATACGGTGATCATGCATCCGCTCCCCCGGGACTCACGTGCCCAGGCCAACGAGCTGGACAATGATTTGAACAGCCATC
CTAACCTGGCCATATTCCGCCAGGCCGATAACGGGCTGCTCATTCGCATGGCGCTGTTTGCCCTGACATTGGGCGTGGAC
TCACAGCTGGAAAAGTACGAGTGTCCGGTTAACTGGTATTCACGCAAGGCTGACTTCTAA

Upstream 100 bases:

>100_bases
GCCCCTTCTGCTATAGTTGCGCCTCAGCCAAACCCTACATAAACCAGGGGCGGAAAGGCCTTGTCGCCACTCCCTCCCAC
AGCAATTGGAAAGCCGGCTG

Downstream 100 bases:

>100_bases
GGTCGGCTACTGACTTAAAGGATTGATAAATGACCCGTTCCGAAACCTTGTTTGAACAGGCTAAAAAAACCATTCCCGGT
GGCGTTAACTCACCGGTGCG

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MTQFKGSHILSVNQFNLDSIHTIFNVAERMAPYALREKRTRVLDGAILGNLFFEPSTRTRVSFGCAFNLLGGRVAETVGM
ASSSLSKGESLYDTARVLSTYSDVIAMRHPDAYSVREFAEGSRVPVINGGDGPNEHPTQALLDLFTIQKELSHAGMSIDG
MHIAMVGDLKFGRTVHSLSRLLCLYKNVSFTLISPKELAMPDYVIADIENAGHKVTITDQLEGNLDKADILYLTRIQEER
FPSQDEANKYRGKFRLNRSIYTQHCKSNTVIMHPLPRDSRAQANELDNDLNSHPNLAIFRQADNGLLIRMALFALTLGVD
SQLEKYECPVNWYSRKADF

Sequences:

>Translated_339_residues
MTQFKGSHILSVNQFNLDSIHTIFNVAERMAPYALREKRTRVLDGAILGNLFFEPSTRTRVSFGCAFNLLGGRVAETVGM
ASSSLSKGESLYDTARVLSTYSDVIAMRHPDAYSVREFAEGSRVPVINGGDGPNEHPTQALLDLFTIQKELSHAGMSIDG
MHIAMVGDLKFGRTVHSLSRLLCLYKNVSFTLISPKELAMPDYVIADIENAGHKVTITDQLEGNLDKADILYLTRIQEER
FPSQDEANKYRGKFRLNRSIYTQHCKSNTVIMHPLPRDSRAQANELDNDLNSHPNLAIFRQADNGLLIRMALFALTLGVD
SQLEKYECPVNWYSRKADF
>Mature_338_residues
TQFKGSHILSVNQFNLDSIHTIFNVAERMAPYALREKRTRVLDGAILGNLFFEPSTRTRVSFGCAFNLLGGRVAETVGMA
SSSLSKGESLYDTARVLSTYSDVIAMRHPDAYSVREFAEGSRVPVINGGDGPNEHPTQALLDLFTIQKELSHAGMSIDGM
HIAMVGDLKFGRTVHSLSRLLCLYKNVSFTLISPKELAMPDYVIADIENAGHKVTITDQLEGNLDKADILYLTRIQEERF
PSQDEANKYRGKFRLNRSIYTQHCKSNTVIMHPLPRDSRAQANELDNDLNSHPNLAIFRQADNGLLIRMALFALTLGVDS
QLEKYECPVNWYSRKADF

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=313, Percent_Identity=45.6869009584665, Blast_Score=254, Evalue=5e-68,
Organism=Homo sapiens, GI38788445, Length=336, Percent_Identity=25.8928571428571, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI2367364, Length=315, Percent_Identity=39.6825396825397, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1786469, Length=343, Percent_Identity=25.9475218658892, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790703, Length=239, Percent_Identity=30.1255230125523, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=328, Percent_Identity=39.3292682926829, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6322331, Length=321, Percent_Identity=42.9906542056075, Blast_Score=248, Evalue=9e-67,
Organism=Saccharomyces cerevisiae, GI6322373, Length=282, Percent_Identity=25.177304964539, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24642586, Length=317, Percent_Identity=43.8485804416404, Blast_Score=248, Evalue=4e-66,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 37993; Mature: 37862

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQFKGSHILSVNQFNLDSIHTIFNVAERMAPYALREKRTRVLDGAILGNLFFEPSTRTR
CCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCE
VSFGCAFNLLGGRVAETVGMASSSLSKGESLYDTARVLSTYSDVIAMRHPDAYSVREFAE
EEECEEEHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHC
GSRVPVINGGDGPNEHPTQALLDLFTIQKELSHAGMSIDGMHIAMVGDLKFGRTVHSLSR
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHH
LLCLYKNVSFTLISPKELAMPDYVIADIENAGHKVTITDQLEGNLDKADILYLTRIQEER
HHHHHCCCEEEEECCHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCHHEEEEEHHHHHH
FPSQDEANKYRGKFRLNRSIYTQHCKSNTVIMHPLPRDSRAQANELDNDLNSHPNLAIFR
CCCCCHHHHHCCEEEECHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCEEEEE
QADNGLLIRMALFALTLGVDSQLEKYECPVNWYSRKADF
ECCCCCHHHHHHHHHHHCCCCCHHHHCCCCHHHHCCCCC
>Mature Secondary Structure 
TQFKGSHILSVNQFNLDSIHTIFNVAERMAPYALREKRTRVLDGAILGNLFFEPSTRTR
CCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCE
VSFGCAFNLLGGRVAETVGMASSSLSKGESLYDTARVLSTYSDVIAMRHPDAYSVREFAE
EEECEEEHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHC
GSRVPVINGGDGPNEHPTQALLDLFTIQKELSHAGMSIDGMHIAMVGDLKFGRTVHSLSR
CCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHH
LLCLYKNVSFTLISPKELAMPDYVIADIENAGHKVTITDQLEGNLDKADILYLTRIQEER
HHHHHCCCEEEEECCHHHCCCCEEEEEECCCCCEEEEEECCCCCCCCHHEEEEEHHHHHH
FPSQDEANKYRGKFRLNRSIYTQHCKSNTVIMHPLPRDSRAQANELDNDLNSHPNLAIFR
CCCCCHHHHHCCEEEECHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCCCCEEEEE
QADNGLLIRMALFALTLGVDSQLEKYECPVNWYSRKADF
ECCCCCHHHHHHHHHHHCCCCCHHHHCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA