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The map label for this gene is oprF [H]

Identifier: 119773836

GI number: 119773836

Start: 861843

End: 863288

Strand: Reverse

Name: oprF [H]

Synonym: Sama_0698

Alternate gene names: 119773836

Gene position: 863288-861843 (Counterclockwise)

Preceding gene: 119773860

Following gene: 119773835

Centisome position: 20.05

GC content: 52.7

Gene sequence:

>1446_bases
ATGGGACAAAATACGTTAATATTTGTATCACTTCTCGCCAGCTTAGCCACATCTGTTAATGCTGCGAGCTTATTTGGTCC
ATTCCCTAATGCAAAGTATCCCGAAGAAAAACGCATCCATTTCACGCCTTTTCACATCATCACGGCCGTCAGTAACCAGG
GCTTCACGCTTAAGCCGGTTTCAGGGCGCCTGACCCACAACTCATTTGAACTTCCGGACAGCTATCCGCTGGCACTGGTA
ATGAATAACTATCTGGCGCAGTTTAAAAACCTCAATGCCGAGGTGTTATTTCAATGTGAGGCTGAAGGCTGCGGCGATGG
CTGGGCCATGCGGGACCAGCTGGCACCCCTGATAACAGTGGATACCAGTTATCCCGGCGCTTACGTCGCAGCAAAGCTTA
AAGGTGCGGGCGGAGATGTCTACAGCAGCATCTACGCCTTTCACCGTGAGGGCAGTTACACCAAGGTGCAGCTGGTTACC
CTTGAAGTCATCCCGGAACCGCTGGATCTTGTTGAGGCAAACAGCAATTTTTTGCAGCAGGGCACACAGGATGTGGCGAT
GGCTGACAGACGCAGTGACGATGCCCAGGACTCAGCCGATCATCCGTTGCTTGGGCGCATGCCAGGCAATTATATCACGC
GTTACAAACAAACCAATCTTACCCAGGTTCCGGTCATAGTAGGCATCTCAGCAGCCGGCATTCAGACCCAATCTCTGGAC
GCCAGGCTCACTGAAATTCGTTATCAGATGCCCGAGCGTTACTCGCTGTTTGAAATTAACAGCAATTACGCCGCTGCTGC
ACAAAAGCTTAACGCAGAGCAGGTGTTTTACTGCAAAGGCACTGGCTGTGGCGATGATGATGCGCTTATCAAAGCCATGG
GATTGTTCCAGGATGATGCCGAAGATCAGTGGCAGGAATATCAGCTGTATAGATTGAGCCGCCCTCAGGGTGATGTGTAT
TTCGATATCTATTCCCAGGGCCGTTACGACGCAACACCGGGGGATACCACGCTCAGAGTTATGGAGCTGTCTGTCCTGAA
AACGGACAGGATCGCCATCAATCTCGACGCCCTCAATGACGCCATCACACAAACCGGCAAGGCAACCCTGGATGGTTTGC
TGTTCGACTTCGACAGCGACCGGATGCTGCCTGAATCCCAGCCGGTGCTGGAGGTGCTGGCAACCTATCTTAAACAGCAC
CCGGCTAAAGGCTTTTACGTGGTTGGCCATACCGATGACAAGGGAGAGCGCGCCTACAACCAAAGTCTGTCGTTGCGCCG
CGCCGATGCCGTTATTCAAAGCCTCACGCAGCAATATCACATCCCGGCGTCACAATTAACCGCCCATGGCAACGGGGAAT
ATAGCCCGGTGGCGAGTAATGAAAACGAGTCAGGCCAGGCACTCAACCGCAGAGTGGAATTGGTGCTGCGTTCGGACATG
GAATAG

Upstream 100 bases:

>100_bases
AACACGCCAAATAAGGACTTTTCTATGTACTTGTTATATGATCGGCGTCCTTGCGCTTATTTACGCTAAAGCAAGAAAGG
CCCGCTGAAAAGGATTTTTT

Downstream 100 bases:

>100_bases
CCAGCTTTTGCAAAGCATCAAAGAATCAAACAGATAAGGATTTACAGCAGTATGAAAAGACATATTTTCACCTGTGCCAT
GGCGATTTATCTTCCGGCCA

Product: Outer membrane protein-related peptidoglycan-associated (lipo)protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MGQNTLIFVSLLASLATSVNAASLFGPFPNAKYPEEKRIHFTPFHIITAVSNQGFTLKPVSGRLTHNSFELPDSYPLALV
MNNYLAQFKNLNAEVLFQCEAEGCGDGWAMRDQLAPLITVDTSYPGAYVAAKLKGAGGDVYSSIYAFHREGSYTKVQLVT
LEVIPEPLDLVEANSNFLQQGTQDVAMADRRSDDAQDSADHPLLGRMPGNYITRYKQTNLTQVPVIVGISAAGIQTQSLD
ARLTEIRYQMPERYSLFEINSNYAAAAQKLNAEQVFYCKGTGCGDDDALIKAMGLFQDDAEDQWQEYQLYRLSRPQGDVY
FDIYSQGRYDATPGDTTLRVMELSVLKTDRIAINLDALNDAITQTGKATLDGLLFDFDSDRMLPESQPVLEVLATYLKQH
PAKGFYVVGHTDDKGERAYNQSLSLRRADAVIQSLTQQYHIPASQLTAHGNGEYSPVASNENESGQALNRRVELVLRSDM
E

Sequences:

>Translated_481_residues
MGQNTLIFVSLLASLATSVNAASLFGPFPNAKYPEEKRIHFTPFHIITAVSNQGFTLKPVSGRLTHNSFELPDSYPLALV
MNNYLAQFKNLNAEVLFQCEAEGCGDGWAMRDQLAPLITVDTSYPGAYVAAKLKGAGGDVYSSIYAFHREGSYTKVQLVT
LEVIPEPLDLVEANSNFLQQGTQDVAMADRRSDDAQDSADHPLLGRMPGNYITRYKQTNLTQVPVIVGISAAGIQTQSLD
ARLTEIRYQMPERYSLFEINSNYAAAAQKLNAEQVFYCKGTGCGDDDALIKAMGLFQDDAEDQWQEYQLYRLSRPQGDVY
FDIYSQGRYDATPGDTTLRVMELSVLKTDRIAINLDALNDAITQTGKATLDGLLFDFDSDRMLPESQPVLEVLATYLKQH
PAKGFYVVGHTDDKGERAYNQSLSLRRADAVIQSLTQQYHIPASQLTAHGNGEYSPVASNENESGQALNRRVELVLRSDM
E
>Mature_480_residues
GQNTLIFVSLLASLATSVNAASLFGPFPNAKYPEEKRIHFTPFHIITAVSNQGFTLKPVSGRLTHNSFELPDSYPLALVM
NNYLAQFKNLNAEVLFQCEAEGCGDGWAMRDQLAPLITVDTSYPGAYVAAKLKGAGGDVYSSIYAFHREGSYTKVQLVTL
EVIPEPLDLVEANSNFLQQGTQDVAMADRRSDDAQDSADHPLLGRMPGNYITRYKQTNLTQVPVIVGISAAGIQTQSLDA
RLTEIRYQMPERYSLFEINSNYAAAAQKLNAEQVFYCKGTGCGDDDALIKAMGLFQDDAEDQWQEYQLYRLSRPQGDVYF
DIYSQGRYDATPGDTTLRVMELSVLKTDRIAINLDALNDAITQTGKATLDGLLFDFDSDRMLPESQPVLEVLATYLKQHP
AKGFYVVGHTDDKGERAYNQSLSLRRADAVIQSLTQQYHIPASQLTAHGNGEYSPVASNENESGQALNRRVELVLRSDME

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1788957, Length=108, Percent_Identity=38.8888888888889, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI48994946, Length=104, Percent_Identity=37.5, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 53253; Mature: 53122

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQNTLIFVSLLASLATSVNAASLFGPFPNAKYPEEKRIHFTPFHIITAVSNQGFTLKPV
CCCCCEEHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEECCEEEEEEECCCCEEEECC
SGRLTHNSFELPDSYPLALVMNNYLAQFKNLNAEVLFQCEAEGCGDGWAMRDQLAPLITV
CCCEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEHHHCCCEEEE
DTSYPGAYVAAKLKGAGGDVYSSIYAFHREGSYTKVQLVTLEVIPEPLDLVEANSNFLQQ
ECCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHEECCCHHHHC
GTQDVAMADRRSDDAQDSADHPLLGRMPGNYITRYKQTNLTQVPVIVGISAAGIQTQSLD
CCHHHHHHHCCCCCCCCCCCCCEECCCCCHHHHHHHCCCCEECCEEEEEECCCCEEHHHH
ARLTEIRYQMPERYSLFEINSNYAAAAQKLNAEQVFYCKGTGCGDDDALIKAMGLFQDDA
HHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCC
EDQWQEYQLYRLSRPQGDVYFDIYSQGRYDATPGDTTLRVMELSVLKTDRIAINLDALND
HHHHHHHHEEEECCCCCCEEEEEECCCCCCCCCCCCEEEEEEEEEEEECEEEEEEHHHHH
AITQTGKATLDGLLFDFDSDRMLPESQPVLEVLATYLKQHPAKGFYVVGHTDDKGERAYN
HHHHCCCHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
QSLSLRRADAVIQSLTQQYHIPASQLTAHGNGEYSPVASNENESGQALNRRVELVLRSDM
HCCHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHEECCC
E
C
>Mature Secondary Structure 
GQNTLIFVSLLASLATSVNAASLFGPFPNAKYPEEKRIHFTPFHIITAVSNQGFTLKPV
CCCCEEHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEECCEEEEEEECCCCEEEECC
SGRLTHNSFELPDSYPLALVMNNYLAQFKNLNAEVLFQCEAEGCGDGWAMRDQLAPLITV
CCCEECCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEHHHCCCEEEE
DTSYPGAYVAAKLKGAGGDVYSSIYAFHREGSYTKVQLVTLEVIPEPLDLVEANSNFLQQ
ECCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHEECCCHHHHC
GTQDVAMADRRSDDAQDSADHPLLGRMPGNYITRYKQTNLTQVPVIVGISAAGIQTQSLD
CCHHHHHHHCCCCCCCCCCCCCEECCCCCHHHHHHHCCCCEECCEEEEEECCCCEEHHHH
ARLTEIRYQMPERYSLFEINSNYAAAAQKLNAEQVFYCKGTGCGDDDALIKAMGLFQDDA
HHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCC
EDQWQEYQLYRLSRPQGDVYFDIYSQGRYDATPGDTTLRVMELSVLKTDRIAINLDALND
HHHHHHHHEEEECCCCCCEEEEEECCCCCCCCCCCCEEEEEEEEEEEECEEEEEEHHHHH
AITQTGKATLDGLLFDFDSDRMLPESQPVLEVLATYLKQHPAKGFYVVGHTDDKGERAYN
HHHHCCCHHHCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHH
QSLSLRRADAVIQSLTQQYHIPASQLTAHGNGEYSPVASNENESGQALNRRVELVLRSDM
HCCHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHEECCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]