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The map label for this gene is dctB [H]

Identifier: 119773687

GI number: 119773687

Start: 673156

End: 674799

Strand: Reverse

Name: dctB [H]

Synonym: Sama_0547

Alternate gene names: 119773687

Gene position: 674799-673156 (Counterclockwise)

Preceding gene: 119773689

Following gene: 119773685

Centisome position: 15.67

GC content: 52.01

Gene sequence:

>1644_bases
ATGCTGGGTTTTTCTCGTCAGGCGGTGAGCAGCAAAATCGGTCGCAGGCTGATGATTTCGATCGTGCTGTTCAGCTCGCT
TATCACACTCATCACCACCGGCTATCAGCTGTTTAACGACTATAACGGCGATCTTAACCGTATCGATCGTGCCTTCTCCA
ACATCGAGAAAGTTAACCTCGACGTGCTGGCGGCCAGTATCTGGGTGATTGATGAGCGCCTTATCAATACGCAACTCGAA
GGCCTTATCCAGCTGCCCGACATTACCTATATCTCCATCGATGACGACAGCGGCCAGCACTGGAGCCGCGGTGCCCCCAT
CGACAAGAACTTTATTGAGAAGGTGTTTTCCCTAAACTACAACTCCGGCAGTGAAAGCATCTCCGTGGGTAAACTCACTA
TCCTGGCCGACTTGAATGCCGTGTACGAACGCATTTATGACAAGGCCATCATCATATTGCTCTCCAACGCCATAAAAACC
TTTTTGGTGGCTGGCATGATCCTTATCCTGGTGTGGCTCAACATTACCCGCCACCTCAATACTCTGGCAGCCTATTGCCA
GGACATCAGCGTAGAACATGAGTATCAGCCACTGACGTTTCAAAAGCGCGGTGCTCAAAATGAGTTCGATCAGGTCGCCC
TGGCAATAAACGAAATGCAGGAACAGCTGCATCGTTCCTTCGGTGCCTTGAAAAAGTCCAAGTCCGACTTGCAACACGCG
CTGGAAGACAGAGAAAGGCTACTGGAACTGGAGCGCTCGTATAAGGAAGAACTCGCCAAGCAGGTAAAAGAGCGCACCAT
GGAGCTGGAACAGTCGCTGTTAATTCTGAAAAGAGCACAGGAGGCGCTGGTTGAGCAGGAGAAAATGGCTGCCCTTGGGG
GCTTGGTGTCCGGTGTTGCCCACGAAATCAACACCCCAATTGGGATTTGCCTCACCGCTGCCAGTACCCAGTTGGCTCAC
ATCGATGAACTCATCAGCCTTATTCACAGTGAAGAAGCCACCCTGGAGGAAATCAACGCCATTCTCGAAGAATACCAGCA
AAGCTGTGAGCTCATCGTCAGCAATATCACCCGCGCCAGTAACCTCATCCAGAAATTCAAAACCATTGCAGCGGAAAACA
GCCATGAGGCTCACGAGCAAATTCCCATTGCCCAGCTGTGTCGGGACATCCATGACTCCACACAGCTTATCTATGCGCCA
ACAATGGCCAACATGGAACTGGATATTGCCGATGAGCTACAGGTAGAAACAAACTACAGTCTGCTCAACCAGATACTGAG
TAATCTGATGTCCAACATCTATGCCCATGCCTTCGCACAGGGGCGGGAAAATCTCTTCCGGGTAGAGGCCTATCTGGAAA
ACCGGCGCCTTGTCGTGCGCCTTGAGGATAATGGCCCCGGGATCGCCGCTGATGTCGCCGAGCATATGTTTGAGCCATTT
TATACCACCATCCGCGCCCGGGGAGGCACTGGGCTGGGCTTGTCAGCGGCATTCAATGCAGCAACCCTGCTTAAAGGGAG
TGTGCGTTACGAAGGAAAGTCAACTCTGGGGGGGGCCTGCTTTGTCCTGAGCATCCCGGTAAAACGGGATGACGATCTGC
AGGCCAGCGACTCAGTCAAAGACGGGTATCAATTCCATATCTAG

Upstream 100 bases:

>100_bases
ATATGTCAATTTGTACTCAGGTTGCAGAATTTCAGCGTTTTTTTATTAGCCAAACTATGCTTATACATATTCAAATGCAG
GTTGGGGCTAATTAGAAAGC

Downstream 100 bases:

>100_bases
GCTTTTTGCTTAAGCCACATCAATACCCGGCTCACCGCCATAAAACCAAAGGTGCCGGTGACCACGGTCACAGCGCCGAA
GCCTGAAGCACAGTCCATCC

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MLGFSRQAVSSKIGRRLMISIVLFSSLITLITTGYQLFNDYNGDLNRIDRAFSNIEKVNLDVLAASIWVIDERLINTQLE
GLIQLPDITYISIDDDSGQHWSRGAPIDKNFIEKVFSLNYNSGSESISVGKLTILADLNAVYERIYDKAIIILLSNAIKT
FLVAGMILILVWLNITRHLNTLAAYCQDISVEHEYQPLTFQKRGAQNEFDQVALAINEMQEQLHRSFGALKKSKSDLQHA
LEDRERLLELERSYKEELAKQVKERTMELEQSLLILKRAQEALVEQEKMAALGGLVSGVAHEINTPIGICLTAASTQLAH
IDELISLIHSEEATLEEINAILEEYQQSCELIVSNITRASNLIQKFKTIAAENSHEAHEQIPIAQLCRDIHDSTQLIYAP
TMANMELDIADELQVETNYSLLNQILSNLMSNIYAHAFAQGRENLFRVEAYLENRRLVVRLEDNGPGIAADVAEHMFEPF
YTTIRARGGTGLGLSAAFNAATLLKGSVRYEGKSTLGGACFVLSIPVKRDDDLQASDSVKDGYQFHI

Sequences:

>Translated_547_residues
MLGFSRQAVSSKIGRRLMISIVLFSSLITLITTGYQLFNDYNGDLNRIDRAFSNIEKVNLDVLAASIWVIDERLINTQLE
GLIQLPDITYISIDDDSGQHWSRGAPIDKNFIEKVFSLNYNSGSESISVGKLTILADLNAVYERIYDKAIIILLSNAIKT
FLVAGMILILVWLNITRHLNTLAAYCQDISVEHEYQPLTFQKRGAQNEFDQVALAINEMQEQLHRSFGALKKSKSDLQHA
LEDRERLLELERSYKEELAKQVKERTMELEQSLLILKRAQEALVEQEKMAALGGLVSGVAHEINTPIGICLTAASTQLAH
IDELISLIHSEEATLEEINAILEEYQQSCELIVSNITRASNLIQKFKTIAAENSHEAHEQIPIAQLCRDIHDSTQLIYAP
TMANMELDIADELQVETNYSLLNQILSNLMSNIYAHAFAQGRENLFRVEAYLENRRLVVRLEDNGPGIAADVAEHMFEPF
YTTIRARGGTGLGLSAAFNAATLLKGSVRYEGKSTLGGACFVLSIPVKRDDDLQASDSVKDGYQFHI
>Mature_547_residues
MLGFSRQAVSSKIGRRLMISIVLFSSLITLITTGYQLFNDYNGDLNRIDRAFSNIEKVNLDVLAASIWVIDERLINTQLE
GLIQLPDITYISIDDDSGQHWSRGAPIDKNFIEKVFSLNYNSGSESISVGKLTILADLNAVYERIYDKAIIILLSNAIKT
FLVAGMILILVWLNITRHLNTLAAYCQDISVEHEYQPLTFQKRGAQNEFDQVALAINEMQEQLHRSFGALKKSKSDLQHA
LEDRERLLELERSYKEELAKQVKERTMELEQSLLILKRAQEALVEQEKMAALGGLVSGVAHEINTPIGICLTAASTQLAH
IDELISLIHSEEATLEEINAILEEYQQSCELIVSNITRASNLIQKFKTIAAENSHEAHEQIPIAQLCRDIHDSTQLIYAP
TMANMELDIADELQVETNYSLLNQILSNLMSNIYAHAFAQGRENLFRVEAYLENRRLVVRLEDNGPGIAADVAEHMFEPF
YTTIRARGGTGLGLSAAFNAATLLKGSVRYEGKSTLGGACFVLSIPVKRDDDLQASDSVKDGYQFHI

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87081816, Length=290, Percent_Identity=27.9310344827586, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788549, Length=261, Percent_Identity=26.4367816091954, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790300, Length=267, Percent_Identity=26.5917602996255, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1787894, Length=111, Percent_Identity=35.1351351351351, Blast_Score=63, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 61236; Mature: 61236

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGFSRQAVSSKIGRRLMISIVLFSSLITLITTGYQLFNDYNGDLNRIDRAFSNIEKVNL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
DVLAASIWVIDERLINTQLEGLIQLPDITYISIDDDSGQHWSRGAPIDKNFIEKVFSLNY
HHHHHHHHHHHHHHHHHHHHCEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCC
NSGSESISVGKLTILADLNAVYERIYDKAIIILLSNAIKTFLVAGMILILVWLNITRHLN
CCCCCCEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLAAYCQDISVEHEYQPLTFQKRGAQNEFDQVALAINEMQEQLHRSFGALKKSKSDLQHA
HHHHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDRERLLELERSYKEELAKQVKERTMELEQSLLILKRAQEALVEQEKMAALGGLVSGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEINTPIGICLTAASTQLAHIDELISLIHSEEATLEEINAILEEYQQSCELIVSNITRAS
HHHCCCHHEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLIQKFKTIAAENSHEAHEQIPIAQLCRDIHDSTQLIYAPTMANMELDIADELQVETNYS
HHHHHHHHHHCCCCCHHHHCCCHHHHHHHHCCCCEEEEECCCCCCEECCHHHHEECCCHH
LLNQILSNLMSNIYAHAFAQGRENLFRVEAYLENRRLVVRLEDNGPGIAADVAEHMFEPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHH
YTTIRARGGTGLGLSAAFNAATLLKGSVRYEGKSTLGGACFVLSIPVKRDDDLQASDSVK
HHHHHCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCCCEEEEEEECCCCCCCCCCCCCCC
DGYQFHI
CCCEECC
>Mature Secondary Structure
MLGFSRQAVSSKIGRRLMISIVLFSSLITLITTGYQLFNDYNGDLNRIDRAFSNIEKVNL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
DVLAASIWVIDERLINTQLEGLIQLPDITYISIDDDSGQHWSRGAPIDKNFIEKVFSLNY
HHHHHHHHHHHHHHHHHHHHCEEECCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCC
NSGSESISVGKLTILADLNAVYERIYDKAIIILLSNAIKTFLVAGMILILVWLNITRHLN
CCCCCCEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLAAYCQDISVEHEYQPLTFQKRGAQNEFDQVALAINEMQEQLHRSFGALKKSKSDLQHA
HHHHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDRERLLELERSYKEELAKQVKERTMELEQSLLILKRAQEALVEQEKMAALGGLVSGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HEINTPIGICLTAASTQLAHIDELISLIHSEEATLEEINAILEEYQQSCELIVSNITRAS
HHHCCCHHEEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NLIQKFKTIAAENSHEAHEQIPIAQLCRDIHDSTQLIYAPTMANMELDIADELQVETNYS
HHHHHHHHHHCCCCCHHHHCCCHHHHHHHHCCCCEEEEECCCCCCEECCHHHHEECCCHH
LLNQILSNLMSNIYAHAFAQGRENLFRVEAYLENRRLVVRLEDNGPGIAADVAEHMFEPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHH
YTTIRARGGTGLGLSAAFNAATLLKGSVRYEGKSTLGGACFVLSIPVKRDDDLQASDSVK
HHHHHCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCCCEEEEEEECCCCCCCCCCCCCCC
DGYQFHI
CCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3671068 [H]