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The map label for this gene is outD [H]

Identifier: 119773594

GI number: 119773594

Start: 559471

End: 561156

Strand: Direct

Name: outD [H]

Synonym: Sama_0454

Alternate gene names: 119773594

Gene position: 559471-561156 (Clockwise)

Preceding gene: 119773593

Following gene: 119773595

Centisome position: 12.99

GC content: 53.8

Gene sequence:

>1686_bases
ATGGCAATTTCTGGTATCAAATACCTTACCCCTCTGCTGTCGCTGTGCCTGATAGCATGCCAAACCACTGACAGGCCCAA
CCCTCAGGCGTCCAAAGAGGCGCTCAGGGAGGTTGTGGCTCAACAAAATGCACAAGCTCAGCCACCGGCCAAATTGCCCG
ATTCAGTGTCCCGCGAATTGGCCGGCAGTAACACAGTATTTGCGCCAACGCTGCCACCGGAGCGCCGTTTCGATGTGGCT
GCCAATGCGGTGGATGCCAGAGTGTTTTTTCCGAGTTTGGTCAAGGGAACGCCTTTCAGCGTGGCCGTACACCCCGATGT
GCAGGGACGGATTTCTCTGTCGCTCAAAGGCGTGACCCTGAGTGAGGCCTTGCAGGTGATTGAAGACCTGTACGGCTATG
AAGTCAGCCATGAGGGCAAGGTGCTAAAGGTATTCCCATCGGGCATGCGAACCGAAACGTTCCCGGTGAACTACCTCTAT
ATGGAACGCATGGGGGTGTCTCTAACGTCTGTGACGTCCGGCCGTATTTCCGACAACAATAACAACAATAACAACGGCAA
TAACAACAACGGCAGCAATGGTAATAACGCTTTTGATAACGGCGGGGCCAACAATAACGGTGTCAACGGCAACAATACCA
ATGGTAACAATACCAACGGCACCTTTATCCAGTCCCGCAATAAAACAGACTTCTGGGGTGAACTGAAAGAAACCCTTGAG
TCCTTGATTGGTGGCACCAGTAACAACCGCAGTGTGGTGGTCACACCCCAGGCCGGCTTGGTGACTGTGCGTGCGCTGCC
GGGTGAGCTGCGTCAGGTGAGGGAGTTTTTGGCCACCGCCGAAACCCATCTGCAGCGTCAGGTGATTTTGGAGGCCAAGG
TGCTTGAGGTGACCCTGTCTGATGGTTATCAGCAGGGTATCCAATGGAACAAGATTGCCGGCAGTGCACTGGCCGATGGC
AACACCAAAATTAATTTCGCCACTTCAGCAGGCAATGAGTTCGGCAATCAAATTTCCAGTGCCTTGGGCGGCGTAACCTC
GTTGTCCATCATAGGTTCAGACTTCGACGCCATGATAAATCTCCTCGATACTCAGGGGGATGTGGATGTGTTGTCGAGCC
CCCGTGTCACCGCCTCCAACAACCAAAAGGCGGTGATCAAGGTGGGCAAGGATGAATACTTTGTCACTGATGTGTCATCC
ACCACAGTGGCGGGCACCACACCTGTGACCAGCCCCGAAGTCGAGCTGACACCCTTTTTCTCCGGTATCGCACTGGATGT
TACCCCACAGATTGACGGCCAGGGCAATGTGCTGCTGCATGTGCATCCCTCGGTTATCGATGTGAAAGAACAAACCAAGA
CCATCAAAATCAGCAACAGCGATCTGGAGCTGCCCCTGGCCCAGAGTGAGATCCGCGAATCGGATACCGTGATTAAAGCC
ATGTCGGGGGATGTGGTGGTGATTGGGGGTCTGATGAAGAGTGAGAGCTTGGAGCTGGTGTCCAAGGTACCTCTGCTTGG
GGATATTCCGTTTCTGGGTGAAGCTTTTACCAACCGCAGTCAGTCTGTTCGTAAAACGGAGCTGGTGATACTGCTCAAAC
CAACCGTGGTCGTAAGCGGCACCTGGCAGAAAGAGCTGGAGCGGTCAAAGGCCTTGTTGGACCGTTGGTATCCCGAGGGC
GAATAA

Upstream 100 bases:

>100_bases
TCGGTAACGCGAGGATAACCGCTATCGGCAGTGATTCAGTGTCCCTGTCCGACGGGAAAACCCTCAGGCTGTTTCAGGCT
ATTACAGAGAGATAATAGAT

Downstream 100 bases:

>100_bases
GGTGTATTTGCAGCACTTTGGTCTTCGGGAGATGCCGTTTGCGCTGACACCGAATACCGGATTTTTTTTCGCACTGGCGC
CCCATGCAGAGGCGCTGCAA

Product: MSHA biogenesis protein MshL

Products: NA

Alternate protein names: Pectic enzymes secretion protein outD [H]

Number of amino acids: Translated: 561; Mature: 560

Protein sequence:

>561_residues
MAISGIKYLTPLLSLCLIACQTTDRPNPQASKEALREVVAQQNAQAQPPAKLPDSVSRELAGSNTVFAPTLPPERRFDVA
ANAVDARVFFPSLVKGTPFSVAVHPDVQGRISLSLKGVTLSEALQVIEDLYGYEVSHEGKVLKVFPSGMRTETFPVNYLY
MERMGVSLTSVTSGRISDNNNNNNNGNNNNGSNGNNAFDNGGANNNGVNGNNTNGNNTNGTFIQSRNKTDFWGELKETLE
SLIGGTSNNRSVVVTPQAGLVTVRALPGELRQVREFLATAETHLQRQVILEAKVLEVTLSDGYQQGIQWNKIAGSALADG
NTKINFATSAGNEFGNQISSALGGVTSLSIIGSDFDAMINLLDTQGDVDVLSSPRVTASNNQKAVIKVGKDEYFVTDVSS
TTVAGTTPVTSPEVELTPFFSGIALDVTPQIDGQGNVLLHVHPSVIDVKEQTKTIKISNSDLELPLAQSEIRESDTVIKA
MSGDVVVIGGLMKSESLELVSKVPLLGDIPFLGEAFTNRSQSVRKTELVILLKPTVVVSGTWQKELERSKALLDRWYPEG
E

Sequences:

>Translated_561_residues
MAISGIKYLTPLLSLCLIACQTTDRPNPQASKEALREVVAQQNAQAQPPAKLPDSVSRELAGSNTVFAPTLPPERRFDVA
ANAVDARVFFPSLVKGTPFSVAVHPDVQGRISLSLKGVTLSEALQVIEDLYGYEVSHEGKVLKVFPSGMRTETFPVNYLY
MERMGVSLTSVTSGRISDNNNNNNNGNNNNGSNGNNAFDNGGANNNGVNGNNTNGNNTNGTFIQSRNKTDFWGELKETLE
SLIGGTSNNRSVVVTPQAGLVTVRALPGELRQVREFLATAETHLQRQVILEAKVLEVTLSDGYQQGIQWNKIAGSALADG
NTKINFATSAGNEFGNQISSALGGVTSLSIIGSDFDAMINLLDTQGDVDVLSSPRVTASNNQKAVIKVGKDEYFVTDVSS
TTVAGTTPVTSPEVELTPFFSGIALDVTPQIDGQGNVLLHVHPSVIDVKEQTKTIKISNSDLELPLAQSEIRESDTVIKA
MSGDVVVIGGLMKSESLELVSKVPLLGDIPFLGEAFTNRSQSVRKTELVILLKPTVVVSGTWQKELERSKALLDRWYPEG
E
>Mature_560_residues
AISGIKYLTPLLSLCLIACQTTDRPNPQASKEALREVVAQQNAQAQPPAKLPDSVSRELAGSNTVFAPTLPPERRFDVAA
NAVDARVFFPSLVKGTPFSVAVHPDVQGRISLSLKGVTLSEALQVIEDLYGYEVSHEGKVLKVFPSGMRTETFPVNYLYM
ERMGVSLTSVTSGRISDNNNNNNNGNNNNGSNGNNAFDNGGANNNGVNGNNTNGNNTNGTFIQSRNKTDFWGELKETLES
LIGGTSNNRSVVVTPQAGLVTVRALPGELRQVREFLATAETHLQRQVILEAKVLEVTLSDGYQQGIQWNKIAGSALADGN
TKINFATSAGNEFGNQISSALGGVTSLSIIGSDFDAMINLLDTQGDVDVLSSPRVTASNNQKAVIKVGKDEYFVTDVSST
TVAGTTPVTSPEVELTPFFSGIALDVTPQIDGQGNVLLHVHPSVIDVKEQTKTIKISNSDLELPLAQSEIRESDTVIKAM
SGDVVVIGGLMKSESLELVSKVPLLGDIPFLGEAFTNRSQSVRKTELVILLKPTVVVSGTWQKELERSKALLDRWYPEGE

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=302, Percent_Identity=28.476821192053, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789793, Length=254, Percent_Identity=27.5590551181102, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 60187; Mature: 60056

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAISGIKYLTPLLSLCLIACQTTDRPNPQASKEALREVVAQQNAQAQPPAKLPDSVSREL
CCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AGSNTVFAPTLPPERRFDVAANAVDARVFFPSLVKGTPFSVAVHPDVQGRISLSLKGVTL
CCCCCEEECCCCCCCCCCHHHCCHHHEEECHHHHCCCCEEEEECCCCCCEEEEEEECCCH
SEALQVIEDLYGYEVSHEGKVLKVFPSGMRTETFPVNYLYMERMGVSLTSVTSGRISDNN
HHHHHHHHHHHCCEECCCCCEEEECCCCCCCCCCCEEEEEEHHCCCEEEEEECCCCCCCC
NNNNNGNNNNGSNGNNAFDNGGANNNGVNGNNTNGNNTNGTFIQSRNKTDFWGELKETLE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
SLIGGTSNNRSVVVTPQAGLVTVRALPGELRQVREFLATAETHLQRQVILEAKVLEVTLS
HHHCCCCCCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEC
DGYQQGIQWNKIAGSALADGNTKINFATSAGNEFGNQISSALGGVTSLSIIGSDFDAMIN
CHHHHCCEEECCCCCEECCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCHHHHHH
LLDTQGDVDVLSSPRVTASNNQKAVIKVGKDEYFVTDVSSTTVAGTTPVTSPEVELTPFF
HHCCCCCEEECCCCCEEECCCCEEEEEECCCCEEEEECCCCEEECCCCCCCCCEEEEECC
SGIALDVTPQIDGQGNVLLHVHPSVIDVKEQTKTIKISNSDLELPLAQSEIRESDTVIKA
CCEEEEEEECCCCCCCEEEEECCCEEECCCCCEEEEEECCCCCCCCCHHHHHHHCCEEEE
MSGDVVVIGGLMKSESLELVSKVPLLGDIPFLGEAFTNRSQSVRKTELVILLKPTVVVSG
CCCCEEEEECCCCCCHHHHHHHCCCCCCCCCHHHHHCCCCCCCEEEEEEEEEECEEEEEC
TWQKELERSKALLDRWYPEGE
CHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AISGIKYLTPLLSLCLIACQTTDRPNPQASKEALREVVAQQNAQAQPPAKLPDSVSREL
CCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
AGSNTVFAPTLPPERRFDVAANAVDARVFFPSLVKGTPFSVAVHPDVQGRISLSLKGVTL
CCCCCEEECCCCCCCCCCHHHCCHHHEEECHHHHCCCCEEEEECCCCCCEEEEEEECCCH
SEALQVIEDLYGYEVSHEGKVLKVFPSGMRTETFPVNYLYMERMGVSLTSVTSGRISDNN
HHHHHHHHHHHCCEECCCCCEEEECCCCCCCCCCCEEEEEEHHCCCEEEEEECCCCCCCC
NNNNNGNNNNGSNGNNAFDNGGANNNGVNGNNTNGNNTNGTFIQSRNKTDFWGELKETLE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHH
SLIGGTSNNRSVVVTPQAGLVTVRALPGELRQVREFLATAETHLQRQVILEAKVLEVTLS
HHHCCCCCCCEEEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEC
DGYQQGIQWNKIAGSALADGNTKINFATSAGNEFGNQISSALGGVTSLSIIGSDFDAMIN
CHHHHCCEEECCCCCEECCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCHHHHHH
LLDTQGDVDVLSSPRVTASNNQKAVIKVGKDEYFVTDVSSTTVAGTTPVTSPEVELTPFF
HHCCCCCEEECCCCCEEECCCCEEEEEECCCCEEEEECCCCEEECCCCCCCCCEEEEECC
SGIALDVTPQIDGQGNVLLHVHPSVIDVKEQTKTIKISNSDLELPLAQSEIRESDTVIKA
CCEEEEEEECCCCCCCEEEEECCCEEECCCCCEEEEEECCCCCCCCCHHHHHHHCCEEEE
MSGDVVVIGGLMKSESLELVSKVPLLGDIPFLGEAFTNRSQSVRKTELVILLKPTVVVSG
CCCCEEEEECCCCCCHHHHHHHCCCCCCCCCHHHHHCCCCCCCEEEEEEEEEECEEEEEC
TWQKELERSKALLDRWYPEGE
CHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]