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The map label for this gene is priA [H]

Identifier: 119773585

GI number: 119773585

Start: 549686

End: 551893

Strand: Reverse

Name: priA [H]

Synonym: Sama_0445

Alternate gene names: 119773585

Gene position: 551893-549686 (Counterclockwise)

Preceding gene: 119773615

Following gene: 119773584

Centisome position: 12.82

GC content: 58.15

Gene sequence:

>2208_bases
ATGTACCTGATTATGCCACAGTTTGTTGAAGTTGCTCTGCCGGTTCCCATGCGCCGCAGTTACAGCTATCGATTGCCAGC
ACAACCAAGCACTTCACCCTGCCCCGGCAAAAGGGTCAAGGTGCCCTTTGGCAAACAACAACTGATAGGTCTTATTACCG
CAACCAAAGACAGTTGCGAGCTGCCCGATGCCCAAATCAAAGAAATCATTGAGATAATCGATGATGAGCCGGCATTGTCT
GAACCACTCTATAAACTGACTCTGTGGGCCGCAAGGTATTATTTTTGCAGTCAGGGACAGATGCTGACCCAGGCATTGCC
GGTGGCACTGCGCAAAGGCGCGGATGCCGCCCCCAAGAGTCGCACCTGGCTTAAATTGACCGACAAGGCCAGAGAGGCCG
GCATCACCACCCTGAAGCGGGCACCAGCCCAACAAAAGCTCGCCGAAATGCTGTGGCAGCATCCCCTGAGTCTGGATGAA
TTCAACGCCATGGAGCTCAGCAAGGCCGCCCTGAAAGGTCTCGAAGACAAGGATTGGGTACAGCGGGAACAGGTGCCGGT
AGACATTAACCTCGGCTGGCGAACAGCGCTTGAGCTTGGTGAAACGCCCCATAAGCTCAACAAGGAACAAGCTGTGGCAG
TCGCTGCCATCAACGCCGCCGAGGGGTACGCGTGCGCACTGCTTGAGGGCATCACGGGCTCAGGTAAAACAGAGGTGTAC
CTTTCGGTATTGGAAAAGATACTGAGCGAAGGCAAACAGGCGCTGATTTTGGTGCCTGAGATAGGCCTGACGCCCCAGAC
CATTGACCGCTTCCGTCGCCGCTTCAAGGTTGAAGTGGCGGTAATCCATTCCAACCTCACCGACAATCAACGCCTCGATG
CCTGGCGTGCAGGGCGCAGCGGCGAAGCTGCCATCATTATCGGTACCCGCAGCGCCTTATTCACGCCCATGGCGTTCCCC
GGCATTATCATCCTCGACGAAGAGCACGACAGCTCGTTCAAACAGCAGGAAGGTGTGGGCTACCACGCCCGCGATCTGGC
GGTGATGCGTGGCCATCTGGAGAATATTCCCGTGGTACTGGGCTCGGCCACACCCTCGCTGGAGTCGCTTCAAAACGCCC
TCTCGGGCCGTTATCGGCACCTGCATCTGGGGGAGCGCGCCGGTAACGCCAAAAAAGTACGTCAGGGCATTATCGATATC
CGCAACCAACCGCTGAAGGCTGGTATGTCGGCGGCGCTGCTCAACGAAATGCGGCTGCATCTGGATGCCGGCAATCAGGT
GCTGCTGTTTTTGAATCGCCGCGGCTTTGCTCCGGCGCTCCTGTGTCACGAGTGCGGCCATTTGCATGAGTGCGATCGCT
GCGATGCCTTTTTTACCGTGCATCAAAAGCTTGGCGAAATTCGCTGCCACCACTGCGGCAACCAATATGCCATTCCGCGC
CAGTGCCACAACTGCGGCTCCACCATGCTGATGGGCCAGGGGATAGGCACAGAGCAGCTGGAACAAGTGCTGAAAGAAGA
GTTCCCCAAATATCCTGTGGTACGCATCGACAGAGACACCACCCGCCTCAAGGGCTCACTGGAAAATCACCTGCAGGCCA
TTCATCGAGGCGACTACAAAATCCTGGTGGGCACCCAGATGCTGGCCAAAGGCCACCACTTCCCGGACGTGACTCTGGTG
GGTCTGTTGGATGTGGATGGTGCCCTGTTCAGCGCCGATTTTCGAGCGCCGGAACGCTTTGGCCAACTTTACACCCAGGT
GGCCGGTCGTGCGGGCCGCGCCGCCAAACCCGGCACTGTGCTGCTGCAAACTCACCAGAGCGATAACCCGATATTACGGG
AGCTGATGCATAAGGGTTATGGCGATTTTGCCCGCGGCCAGCTCAGTGAGCGCAAACAGGCGGCACTGCCACCGGCGTGG
CACATGGTGCTGGCACGGGCCGAAGCCCACAGGGCTGAAGATGCCGAAGCCTTTTTGGCAGCCATCGCGGCCATGTTGCC
AAGGGACAGGGGATTTGAAGTGATTGGGCCCCTGCCCGCGCCCATGGACCGCAAGGCTGGCAAGCACCGACGCCAGCTGA
TGTTTCAGGCCGAGCAGCGTCAGGGATTACAGCAGGCATTGGAAGCGATTCTGCCCGCCATCGATCTTCTGCCCGAGGCA
AAGCGTTGTCGGTGGAGCCTGGACAGAGATCCGCAGGACCTGCTCTAA

Upstream 100 bases:

>100_bases
GCATCGTTTTCTTGCATTGTTTTTCTTGCATAGTTACTGGGCTGCATTAGCTGTGGCTGAGTTCCCACGCTGCCGTTACA
CTAGAGGCAACTCAAGCCAG

Downstream 100 bases:

>100_bases
CCTCCGCGCCACCAAACCTGTAATTAACTGATTTAAAAAGATTCAAATCTTTTCAAAAAGAATCACATCTCACTTTTATC
GGTCTTTCTTATGCTGTCAT

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 735; Mature: 735

Protein sequence:

>735_residues
MYLIMPQFVEVALPVPMRRSYSYRLPAQPSTSPCPGKRVKVPFGKQQLIGLITATKDSCELPDAQIKEIIEIIDDEPALS
EPLYKLTLWAARYYFCSQGQMLTQALPVALRKGADAAPKSRTWLKLTDKAREAGITTLKRAPAQQKLAEMLWQHPLSLDE
FNAMELSKAALKGLEDKDWVQREQVPVDINLGWRTALELGETPHKLNKEQAVAVAAINAAEGYACALLEGITGSGKTEVY
LSVLEKILSEGKQALILVPEIGLTPQTIDRFRRRFKVEVAVIHSNLTDNQRLDAWRAGRSGEAAIIIGTRSALFTPMAFP
GIIILDEEHDSSFKQQEGVGYHARDLAVMRGHLENIPVVLGSATPSLESLQNALSGRYRHLHLGERAGNAKKVRQGIIDI
RNQPLKAGMSAALLNEMRLHLDAGNQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQKLGEIRCHHCGNQYAIPR
QCHNCGSTMLMGQGIGTEQLEQVLKEEFPKYPVVRIDRDTTRLKGSLENHLQAIHRGDYKILVGTQMLAKGHHFPDVTLV
GLLDVDGALFSADFRAPERFGQLYTQVAGRAGRAAKPGTVLLQTHQSDNPILRELMHKGYGDFARGQLSERKQAALPPAW
HMVLARAEAHRAEDAEAFLAAIAAMLPRDRGFEVIGPLPAPMDRKAGKHRRQLMFQAEQRQGLQQALEAILPAIDLLPEA
KRCRWSLDRDPQDLL

Sequences:

>Translated_735_residues
MYLIMPQFVEVALPVPMRRSYSYRLPAQPSTSPCPGKRVKVPFGKQQLIGLITATKDSCELPDAQIKEIIEIIDDEPALS
EPLYKLTLWAARYYFCSQGQMLTQALPVALRKGADAAPKSRTWLKLTDKAREAGITTLKRAPAQQKLAEMLWQHPLSLDE
FNAMELSKAALKGLEDKDWVQREQVPVDINLGWRTALELGETPHKLNKEQAVAVAAINAAEGYACALLEGITGSGKTEVY
LSVLEKILSEGKQALILVPEIGLTPQTIDRFRRRFKVEVAVIHSNLTDNQRLDAWRAGRSGEAAIIIGTRSALFTPMAFP
GIIILDEEHDSSFKQQEGVGYHARDLAVMRGHLENIPVVLGSATPSLESLQNALSGRYRHLHLGERAGNAKKVRQGIIDI
RNQPLKAGMSAALLNEMRLHLDAGNQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQKLGEIRCHHCGNQYAIPR
QCHNCGSTMLMGQGIGTEQLEQVLKEEFPKYPVVRIDRDTTRLKGSLENHLQAIHRGDYKILVGTQMLAKGHHFPDVTLV
GLLDVDGALFSADFRAPERFGQLYTQVAGRAGRAAKPGTVLLQTHQSDNPILRELMHKGYGDFARGQLSERKQAALPPAW
HMVLARAEAHRAEDAEAFLAAIAAMLPRDRGFEVIGPLPAPMDRKAGKHRRQLMFQAEQRQGLQQALEAILPAIDLLPEA
KRCRWSLDRDPQDLL
>Mature_735_residues
MYLIMPQFVEVALPVPMRRSYSYRLPAQPSTSPCPGKRVKVPFGKQQLIGLITATKDSCELPDAQIKEIIEIIDDEPALS
EPLYKLTLWAARYYFCSQGQMLTQALPVALRKGADAAPKSRTWLKLTDKAREAGITTLKRAPAQQKLAEMLWQHPLSLDE
FNAMELSKAALKGLEDKDWVQREQVPVDINLGWRTALELGETPHKLNKEQAVAVAAINAAEGYACALLEGITGSGKTEVY
LSVLEKILSEGKQALILVPEIGLTPQTIDRFRRRFKVEVAVIHSNLTDNQRLDAWRAGRSGEAAIIIGTRSALFTPMAFP
GIIILDEEHDSSFKQQEGVGYHARDLAVMRGHLENIPVVLGSATPSLESLQNALSGRYRHLHLGERAGNAKKVRQGIIDI
RNQPLKAGMSAALLNEMRLHLDAGNQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQKLGEIRCHHCGNQYAIPR
QCHNCGSTMLMGQGIGTEQLEQVLKEEFPKYPVVRIDRDTTRLKGSLENHLQAIHRGDYKILVGTQMLAKGHHFPDVTLV
GLLDVDGALFSADFRAPERFGQLYTQVAGRAGRAAKPGTVLLQTHQSDNPILRELMHKGYGDFARGQLSERKQAALPPAW
HMVLARAEAHRAEDAEAFLAAIAAMLPRDRGFEVIGPLPAPMDRKAGKHRRQLMFQAEQRQGLQQALEAILPAIDLLPEA
KRCRWSLDRDPQDLL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=733, Percent_Identity=50.6139154160982, Blast_Score=728, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81764; Mature: 81764

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLIMPQFVEVALPVPMRRSYSYRLPAQPSTSPCPGKRVKVPFGKQQLIGLITATKDSCE
CEEECCCHHHHHCCCCCCCCCCEECCCCCCCCCCCCCEEECCCCHHHHHEEEEECCCCCC
LPDAQIKEIIEIIDDEPALSEPLYKLTLWAARYYFCSQGQMLTQALPVALRKGADAAPKS
CCHHHHHHHHHHHCCCCCHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
RTWLKLTDKAREAGITTLKRAPAQQKLAEMLWQHPLSLDEFNAMELSKAALKGLEDKDWV
CCEEEECHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHH
QREQVPVDINLGWRTALELGETPHKLNKEQAVAVAAINAAEGYACALLEGITGSGKTEVY
HHHCCCEEECCCHHHHHHCCCCCCCCCHHHEEEEEEEECCCCHHHHHHHCCCCCCCHHHH
LSVLEKILSEGKQALILVPEIGLTPQTIDRFRRRFKVEVAVIHSNLTDNQRLDAWRAGRS
HHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHCCCC
GEAAIIIGTRSALFTPMAFPGIIILDEEHDSSFKQQEGVGYHARDLAVMRGHLENIPVVL
CCEEEEEECCHHHHCCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCEEE
GSATPSLESLQNALSGRYRHLHLGERAGNAKKVRQGIIDIRNQPLKAGMSAALLNEMRLH
ECCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHE
LDAGNQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQKLGEIRCHHCGNQYAIPR
ECCCCEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
QCHNCGSTMLMGQGIGTEQLEQVLKEEFPKYPVVRIDRDTTRLKGSLENHLQAIHRGDYK
HHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEE
ILVGTQMLAKGHHFPDVTLVGLLDVDGALFSADFRAPERFGQLYTQVAGRAGRAAKPGTV
EEEEHHHHHCCCCCCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEE
LLQTHQSDNPILRELMHKGYGDFARGQLSERKQAALPPAWHMVLARAEAHRAEDAEAFLA
EEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHH
AIAAMLPRDRGFEVIGPLPAPMDRKAGKHRRQLMFQAEQRQGLQQALEAILPAIDLLPEA
HHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
KRCRWSLDRDPQDLL
HHHCCCCCCCHHHCC
>Mature Secondary Structure
MYLIMPQFVEVALPVPMRRSYSYRLPAQPSTSPCPGKRVKVPFGKQQLIGLITATKDSCE
CEEECCCHHHHHCCCCCCCCCCEECCCCCCCCCCCCCEEECCCCHHHHHEEEEECCCCCC
LPDAQIKEIIEIIDDEPALSEPLYKLTLWAARYYFCSQGQMLTQALPVALRKGADAAPKS
CCHHHHHHHHHHHCCCCCHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCC
RTWLKLTDKAREAGITTLKRAPAQQKLAEMLWQHPLSLDEFNAMELSKAALKGLEDKDWV
CCEEEECHHHHHCCCHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHH
QREQVPVDINLGWRTALELGETPHKLNKEQAVAVAAINAAEGYACALLEGITGSGKTEVY
HHHCCCEEECCCHHHHHHCCCCCCCCCHHHEEEEEEEECCCCHHHHHHHCCCCCCCHHHH
LSVLEKILSEGKQALILVPEIGLTPQTIDRFRRRFKVEVAVIHSNLTDNQRLDAWRAGRS
HHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHCCCC
GEAAIIIGTRSALFTPMAFPGIIILDEEHDSSFKQQEGVGYHARDLAVMRGHLENIPVVL
CCEEEEEECCHHHHCCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCEEE
GSATPSLESLQNALSGRYRHLHLGERAGNAKKVRQGIIDIRNQPLKAGMSAALLNEMRLH
ECCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHE
LDAGNQVLLFLNRRGFAPALLCHECGHLHECDRCDAFFTVHQKLGEIRCHHCGNQYAIPR
ECCCCEEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
QCHNCGSTMLMGQGIGTEQLEQVLKEEFPKYPVVRIDRDTTRLKGSLENHLQAIHRGDYK
HHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCEE
ILVGTQMLAKGHHFPDVTLVGLLDVDGALFSADFRAPERFGQLYTQVAGRAGRAAKPGTV
EEEEHHHHHCCCCCCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEE
LLQTHQSDNPILRELMHKGYGDFARGQLSERKQAALPPAWHMVLARAEAHRAEDAEAFLA
EEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHH
AIAAMLPRDRGFEVIGPLPAPMDRKAGKHRRQLMFQAEQRQGLQQALEAILPAIDLLPEA
HHHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
KRCRWSLDRDPQDLL
HHHCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]