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The map label for this gene is purD [H]

Identifier: 119773534

GI number: 119773534

Start: 489862

End: 491157

Strand: Reverse

Name: purD [H]

Synonym: Sama_0394

Alternate gene names: 119773534

Gene position: 491157-489862 (Counterclockwise)

Preceding gene: 119773535

Following gene: 119773533

Centisome position: 11.41

GC content: 59.41

Gene sequence:

>1296_bases
ATGAATATTCTCGTAATTGGCAGTGGTGGCCGCGAACACGCCCTGGCCTGGAAAGCCGCTCAGAATGCCGACGTAAATAC
CGTATTTGTGGCCCCCGGCAACGCCGGTTCAAGCCTCGAGCCCAAAGTGGAAAACGTGGCCATTGACGCCACCGACATCC
CTGCGCTGCTCGCCTTTGCCAAACAAAGCAATATCGCCCTGACCATAGTTGGCCCGGAAGCGCCGCTGGTGATTGGTGTT
GTGGATGCGTTTCGTGCCGAAGGCCTGACCATTTTTGGTCCTACCCAGGGCGCCGCTCAGCTGGAAGGCTCCAAGGCCTT
CACCAAGGACTTTTTGGCCCGTCATCAGATCCCAACCGCGGATTACCAAAACTTCACCGAAATCCCGCCCGCCAAGGCCT
TTGTGGCCGAGCTTGCCTGCAAGACCGGTTTCCCTGTGGTTATCAAGGCCGATGGTCTGGCCGCAGGTAAGGGCGTGATT
ATCGCTCAGAGCCAGGCCGAGGCCGATGCCGCCATCGACGATATGCTGGCTGGCAACAAGTTCGGCGAAGCCGGTTCCCG
CGTGGTAGTAGAAGAATTCCTCAAGGGTGAAGAAGCCAGCTTTATCGTGATGGTGGACGGCAAAAACATTCTGCCCATGG
CCACCAGCCAGGATCACAAGGCCCGCGATAACGCCGACCATGGTCCCAACACCGGTGGCATGGGTGCCTACTCCCCAGCC
CCTGTGGTGACTCAGGACGTACACGACTGGGTGATGGCCAACGTTATTCGCCCAACTGTGGATGGCATGGCCGCTGAAGG
CAATGTCTACACCGGCTTCCTGTATGCCGGTCTGATGGTCGACCCTCAGGGCCAGGCCAAGGTGCTGGAATACAACTGCC
GCTTCGGCGACCCCGAGACTCAGCCCATCATGATGCGTCTGAAGTCTGATCTGGTTGAGCTGTGTCTGGCCGCCTGCCGT
GGTGAGTTGGACCAGGTGACCGCTGAGTACGACAGCCGCGCCGCCGTGGGTGTGGTGCTGGCCGCCGGTGGTTATCCGGA
TGAGTACCGCAAGGGCGATGTGATTGAAGGTCTGTCCCTTGGCAACAACGACGCCAAGGTGTTCCATGCCGGCACCAAGA
TGGTCGATGGCCGCGTGGTGACCAACGGCGGCCGCGTACTGTGTGCCACCGCCCTTGGTAACACAGTCACCGAGGCGCAA
AAAGCCGCCTATGCTCTGGTGGATGAAATTCACTGGGACGATGTCTACTTCCGTACCGACATCGGCTATCGCGCCATTGC
CCGCGAAAAGCAGTAA

Upstream 100 bases:

>100_bases
CATGGCCATGGTGTTCACCAACATGCGCCACTTCCGCCACTGATAATATTGAGGCAGCCCTAGGGCTGCCTTTCGCATTC
AAGATTTAAGGAATAAGCAG

Downstream 100 bases:

>100_bases
ATACCCGTCCTTGAGAAAGCCGGCATGCGCCGGCTTTTTTTGTGCCTGCATTTTGACGATCGTCAAAGATACCACCCTGT
AAATCAATGAATTTATTATA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNILVIGSGGREHALAWKAAQNADVNTVFVAPGNAGSSLEPKVENVAIDATDIPALLAFAKQSNIALTIVGPEAPLVIGV
VDAFRAEGLTIFGPTQGAAQLEGSKAFTKDFLARHQIPTADYQNFTEIPPAKAFVAELACKTGFPVVIKADGLAAGKGVI
IAQSQAEADAAIDDMLAGNKFGEAGSRVVVEEFLKGEEASFIVMVDGKNILPMATSQDHKARDNADHGPNTGGMGAYSPA
PVVTQDVHDWVMANVIRPTVDGMAAEGNVYTGFLYAGLMVDPQGQAKVLEYNCRFGDPETQPIMMRLKSDLVELCLAACR
GELDQVTAEYDSRAAVGVVLAAGGYPDEYRKGDVIEGLSLGNNDAKVFHAGTKMVDGRVVTNGGRVLCATALGNTVTEAQ
KAAYALVDEIHWDDVYFRTDIGYRAIAREKQ

Sequences:

>Translated_431_residues
MNILVIGSGGREHALAWKAAQNADVNTVFVAPGNAGSSLEPKVENVAIDATDIPALLAFAKQSNIALTIVGPEAPLVIGV
VDAFRAEGLTIFGPTQGAAQLEGSKAFTKDFLARHQIPTADYQNFTEIPPAKAFVAELACKTGFPVVIKADGLAAGKGVI
IAQSQAEADAAIDDMLAGNKFGEAGSRVVVEEFLKGEEASFIVMVDGKNILPMATSQDHKARDNADHGPNTGGMGAYSPA
PVVTQDVHDWVMANVIRPTVDGMAAEGNVYTGFLYAGLMVDPQGQAKVLEYNCRFGDPETQPIMMRLKSDLVELCLAACR
GELDQVTAEYDSRAAVGVVLAAGGYPDEYRKGDVIEGLSLGNNDAKVFHAGTKMVDGRVVTNGGRVLCATALGNTVTEAQ
KAAYALVDEIHWDDVYFRTDIGYRAIAREKQ
>Mature_431_residues
MNILVIGSGGREHALAWKAAQNADVNTVFVAPGNAGSSLEPKVENVAIDATDIPALLAFAKQSNIALTIVGPEAPLVIGV
VDAFRAEGLTIFGPTQGAAQLEGSKAFTKDFLARHQIPTADYQNFTEIPPAKAFVAELACKTGFPVVIKADGLAAGKGVI
IAQSQAEADAAIDDMLAGNKFGEAGSRVVVEEFLKGEEASFIVMVDGKNILPMATSQDHKARDNADHGPNTGGMGAYSPA
PVVTQDVHDWVMANVIRPTVDGMAAEGNVYTGFLYAGLMVDPQGQAKVLEYNCRFGDPETQPIMMRLKSDLVELCLAACR
GELDQVTAEYDSRAAVGVVLAAGGYPDEYRKGDVIEGLSLGNNDAKVFHAGTKMVDGRVVTNGGRVLCATALGNTVTEAQ
KAAYALVDEIHWDDVYFRTDIGYRAIAREKQ

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=436, Percent_Identity=47.2477064220183, Blast_Score=377, Evalue=1e-105,
Organism=Homo sapiens, GI4503915, Length=436, Percent_Identity=47.2477064220183, Blast_Score=377, Evalue=1e-105,
Organism=Homo sapiens, GI209869995, Length=436, Percent_Identity=47.2477064220183, Blast_Score=377, Evalue=1e-105,
Organism=Homo sapiens, GI209869993, Length=436, Percent_Identity=47.2477064220183, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1790438, Length=433, Percent_Identity=71.5935334872979, Blast_Score=627, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=426, Percent_Identity=40.3755868544601, Blast_Score=300, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=42.9213483146067, Blast_Score=327, Evalue=2e-90,
Organism=Drosophila melanogaster, GI62471619, Length=434, Percent_Identity=43.778801843318, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24582400, Length=434, Percent_Identity=43.778801843318, Blast_Score=328, Evalue=4e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45560; Mature: 45560

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVIGSGGREHALAWKAAQNADVNTVFVAPGNAGSSLEPKVENVAIDATDIPALLAFA
CEEEEEECCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCHHEEEECCCCHHHHHHHH
KQSNIALTIVGPEAPLVIGVVDAFRAEGLTIFGPTQGAAQLEGSKAFTKDFLARHQIPTA
CCCCEEEEEECCCCCEEEEEHHHHHHCCEEEECCCCCCCEECCCHHHHHHHHHHCCCCCC
DYQNFTEIPPAKAFVAELACKTGFPVVIKADGLAAGKGVIIAQSQAEADAAIDDMLAGNK
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCC
FGEAGSRVVVEEFLKGEEASFIVMVDGKNILPMATSQDHKARDNADHGPNTGGMGAYSPA
CCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
PVVTQDVHDWVMANVIRPTVDGMAAEGNVYTGFLYAGLMVDPQGQAKVLEYNCRFGDPET
CCCHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEEEEEEECCCCCEEEEEEECCCCCCCC
QPIMMRLKSDLVELCLAACRGELDQVTAEYDSRAAVGVVLAAGGYPDEYRKGDVIEGLSL
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEEECCCCHHHCCCCCEECEEC
GNNDAKVFHAGTKMVDGRVVTNGGRVLCATALGNTVTEAQKAAYALVDEIHWDDVYFRTD
CCCCCEEEECCCEEECCEEEECCCCEEEEEHHCCHHHHHHHHHHHHHHHCCCCCEEEEEC
IGYRAIAREKQ
CCHHHHHCCCC
>Mature Secondary Structure
MNILVIGSGGREHALAWKAAQNADVNTVFVAPGNAGSSLEPKVENVAIDATDIPALLAFA
CEEEEEECCCCCCEEEEECCCCCCCEEEEEECCCCCCCCCCCHHEEEECCCCHHHHHHHH
KQSNIALTIVGPEAPLVIGVVDAFRAEGLTIFGPTQGAAQLEGSKAFTKDFLARHQIPTA
CCCCEEEEEECCCCCEEEEEHHHHHHCCEEEECCCCCCCEECCCHHHHHHHHHHCCCCCC
DYQNFTEIPPAKAFVAELACKTGFPVVIKADGLAAGKGVIIAQSQAEADAAIDDMLAGNK
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHHHCCCC
FGEAGSRVVVEEFLKGEEASFIVMVDGKNILPMATSQDHKARDNADHGPNTGGMGAYSPA
CCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
PVVTQDVHDWVMANVIRPTVDGMAAEGNVYTGFLYAGLMVDPQGQAKVLEYNCRFGDPET
CCCHHHHHHHHHHHHHHHCCCCEECCCCEEEEEEEEEEEECCCCCEEEEEEECCCCCCCC
QPIMMRLKSDLVELCLAACRGELDQVTAEYDSRAAVGVVLAAGGYPDEYRKGDVIEGLSL
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEEEEEEEECCCCHHHCCCCCEECEEC
GNNDAKVFHAGTKMVDGRVVTNGGRVLCATALGNTVTEAQKAAYALVDEIHWDDVYFRTD
CCCCCEEEECCCEEECCEEEECCCCEEEEEHHCCHHHHHHHHHHHHHHHCCCCCEEEEEC
IGYRAIAREKQ
CCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]