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The map label for this gene is purH

Identifier: 119773535

GI number: 119773535

Start: 491215

End: 492804

Strand: Reverse

Name: purH

Synonym: Sama_0395

Alternate gene names: 119773535

Gene position: 492804-491215 (Counterclockwise)

Preceding gene: 119773540

Following gene: 119773534

Centisome position: 11.44

GC content: 59.69

Gene sequence:

>1590_bases
ATGAATAATGCCAGACCCATTCGCCGCGCGCTGCTGAGCGTGTCCGATAAAACAGGTATCCTCGAGTTTGCCCAGGCGCT
GCACGCACAGGGCGTAGAACTGCTGTCCACCGGTGGCACCGCCAAGCTGCTGGCCGATAACGGCGTACCTGTGATCGAAG
TTTCTGACTACACAGGTCACCCTGAGATCATGGACGGTCGGGTCAAGACCCTGCATCCCAAGGTGCATGGTGGCATTCTG
GCGCGTCGCGGCCAGGACGAAGACGTGATGGCTGCCAACAACATTGGCCCTATCGATCTGGTTGCCGTTAACCTGTATCC
CTTTGCTGCCACCGTAGCCAAGCCTGGCTGTACCCTGGAAGACGCCATCGAGAACATCGATATCGGCGGCCCTACCATGG
TGCGCGCTGCCGCCAAGAACCACAAAGACGTGGTGATTGTGGTGAACGCCAAAGACTACGACCGCGTACTGGCCGAAATG
AGCGCCAATGGCGGCTCTACCAGCCACGCTACCCGTTTCGATTTGGCCATTGCCGCCTTCGAGCACACCGCCGCTTACGA
TGGCATGATTGCCAACTACTTCGGCACCATGGTTCCGGCTCACAGCAGCGACGAGTGCCACGACGACTCCAAATTCCCAC
GCACCTTCAACACCCAGCTGGTGAAGAAGCAGGACCTGCGCTACGGCGAAAACAGCCACCAGAGCGCCGCCTTCTACGTG
GATTTGAACAGCGACGAGGCCTCTGTGGCCACCGCCACTCAGCTGCAGGGTAAGGCTCTGTCTTACAACAACATCGCCGA
CACCGATGCCGCCCTTGAGTGCGTAAAAGAATTCGACGCCCCAGCCTGCGTTATCGTCAAGCACGCCAACCCCTGTGGTG
TGGCCCTGGGCGACAACCTGCTGGACGCGTACAACCGCGCCTACAAGACCGACCCCACTTCTGCTTTCGGTGGCATCATC
GCCTTTAACCGCGAGCTGGACGGCGAAACCGCCGCCGCCATCGTTGAGCGTCAGTTTGTGGAAGTGATTATCGCCCCTGT
GGTGAGCCAAGCCGCCCGTGACGTGGTTGCCAAGAAGACCAACGTGCGCCTGCTGGAATGTGGTCAATGGACTGCGCAGA
CCAAGGGTCTGGACTACAAGCGCGTAAACGGCGGCCTGCTTATTCAGGACCGCGATCAGGGTATGGTGACCGAGGCCGAA
CTCAAGGTCGTGACCAAGCGTGTACCGACCGAAGCTGAACTGAAAGATCTGATGTTCTGCTGGAAAGTGGCCAAGTTCGT
GAAATCCAACGCCATCGTGTATGCCAAAGAAGGCATGACCATAGGCGTGGGCGCAGGCCAGATGAGCCGCGTCTACAGTG
CCAAGATTGCCGGTATCAAGGCCGCCGACGAAGGTCTGGTGGTTGAGGGCTCTGTGATGGCGTCCGACGCCTTCTTCCCA
TTCCGCGACGGTATCGACGCCGCAGCGGCTGCCGGGATCAGCTGCATCATCCAGCCCGGCGGTTCTATCCGCGACGAGGA
AGTGATTGCCGCCGCCGACGAGCACGGCATGGCCATGGTGTTCACCAACATGCGCCACTTCCGCCACTGA

Upstream 100 bases:

>100_bases
CGTAAGGGGAACGCGAGGGGCTTTTGGAAAATAAAACCTTGATGTTGTTTTGTTTTCCGAAAGATCCTTGAACCCTTAAC
AGCGGAAAACTGTACCCAAA

Downstream 100 bases:

>100_bases
TAATATTGAGGCAGCCCTAGGGCTGCCTTTCGCATTCAAGATTTAAGGAATAAGCAGATGAATATTCTCGTAATTGGCAG
TGGTGGCCGCGAACACGCCC

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MNNARPIRRALLSVSDKTGILEFAQALHAQGVELLSTGGTAKLLADNGVPVIEVSDYTGHPEIMDGRVKTLHPKVHGGIL
ARRGQDEDVMAANNIGPIDLVAVNLYPFAATVAKPGCTLEDAIENIDIGGPTMVRAAAKNHKDVVIVVNAKDYDRVLAEM
SANGGSTSHATRFDLAIAAFEHTAAYDGMIANYFGTMVPAHSSDECHDDSKFPRTFNTQLVKKQDLRYGENSHQSAAFYV
DLNSDEASVATATQLQGKALSYNNIADTDAALECVKEFDAPACVIVKHANPCGVALGDNLLDAYNRAYKTDPTSAFGGII
AFNRELDGETAAAIVERQFVEVIIAPVVSQAARDVVAKKTNVRLLECGQWTAQTKGLDYKRVNGGLLIQDRDQGMVTEAE
LKVVTKRVPTEAELKDLMFCWKVAKFVKSNAIVYAKEGMTIGVGAGQMSRVYSAKIAGIKAADEGLVVEGSVMASDAFFP
FRDGIDAAAAAGISCIIQPGGSIRDEEVIAAADEHGMAMVFTNMRHFRH

Sequences:

>Translated_529_residues
MNNARPIRRALLSVSDKTGILEFAQALHAQGVELLSTGGTAKLLADNGVPVIEVSDYTGHPEIMDGRVKTLHPKVHGGIL
ARRGQDEDVMAANNIGPIDLVAVNLYPFAATVAKPGCTLEDAIENIDIGGPTMVRAAAKNHKDVVIVVNAKDYDRVLAEM
SANGGSTSHATRFDLAIAAFEHTAAYDGMIANYFGTMVPAHSSDECHDDSKFPRTFNTQLVKKQDLRYGENSHQSAAFYV
DLNSDEASVATATQLQGKALSYNNIADTDAALECVKEFDAPACVIVKHANPCGVALGDNLLDAYNRAYKTDPTSAFGGII
AFNRELDGETAAAIVERQFVEVIIAPVVSQAARDVVAKKTNVRLLECGQWTAQTKGLDYKRVNGGLLIQDRDQGMVTEAE
LKVVTKRVPTEAELKDLMFCWKVAKFVKSNAIVYAKEGMTIGVGAGQMSRVYSAKIAGIKAADEGLVVEGSVMASDAFFP
FRDGIDAAAAAGISCIIQPGGSIRDEEVIAAADEHGMAMVFTNMRHFRH
>Mature_529_residues
MNNARPIRRALLSVSDKTGILEFAQALHAQGVELLSTGGTAKLLADNGVPVIEVSDYTGHPEIMDGRVKTLHPKVHGGIL
ARRGQDEDVMAANNIGPIDLVAVNLYPFAATVAKPGCTLEDAIENIDIGGPTMVRAAAKNHKDVVIVVNAKDYDRVLAEM
SANGGSTSHATRFDLAIAAFEHTAAYDGMIANYFGTMVPAHSSDECHDDSKFPRTFNTQLVKKQDLRYGENSHQSAAFYV
DLNSDEASVATATQLQGKALSYNNIADTDAALECVKEFDAPACVIVKHANPCGVALGDNLLDAYNRAYKTDPTSAFGGII
AFNRELDGETAAAIVERQFVEVIIAPVVSQAARDVVAKKTNVRLLECGQWTAQTKGLDYKRVNGGLLIQDRDQGMVTEAE
LKVVTKRVPTEAELKDLMFCWKVAKFVKSNAIVYAKEGMTIGVGAGQMSRVYSAKIAGIKAADEGLVVEGSVMASDAFFP
FRDGIDAAAAAGISCIIQPGGSIRDEEVIAAADEHGMAMVFTNMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=492, Percent_Identity=39.6341463414634, Blast_Score=272, Evalue=5e-73,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=75.8034026465028, Blast_Score=840, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=514, Percent_Identity=34.0466926070039, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI71985574, Length=374, Percent_Identity=25.9358288770053, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71985556, Length=119, Percent_Identity=34.453781512605, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323768, Length=486, Percent_Identity=36.6255144032922, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6323056, Length=490, Percent_Identity=35.7142857142857, Blast_Score=254, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24649832, Length=614, Percent_Identity=36.3192182410423, Blast_Score=308, Evalue=5e-84,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_SHEAM (A1S2J9)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926275.1
- ProteinModelPortal:   A1S2J9
- SMR:   A1S2J9
- STRING:   A1S2J9
- GeneID:   4602650
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0395
- NMPDR:   fig|326297.7.peg.375
- eggNOG:   COG0138
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- PhylomeDB:   A1S2J9
- ProtClustDB:   PRK00881
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 56678; Mature: 56678

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNARPIRRALLSVSDKTGILEFAQALHAQGVELLSTGGTAKLLADNGVPVIEVSDYTGH
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEECCCCCC
PEIMDGRVKTLHPKVHGGILARRGQDEDVMAANNIGPIDLVAVNLYPFAATVAKPGCTLE
CCCCCCCEEEECCHHHCCEEECCCCCCCEEEECCCCCEEEEEEEEEEHHHHHCCCCCCHH
DAIENIDIGGPTMVRAAAKNHKDVVIVVNAKDYDRVLAEMSANGGSTSHATRFDLAIAAF
HHHHHCCCCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEEEHH
EHTAAYDGMIANYFGTMVPAHSSDECHDDSKFPRTFNTQLVKKQDLRYGENSHQSAAFYV
HHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEE
DLNSDEASVATATQLQGKALSYNNIADTDAALECVKEFDAPACVIVKHANPCGVALGDNL
EECCCCCHHEEHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEECCHHH
LDAYNRAYKTDPTSAFGGIIAFNRELDGETAAAIVERQFVEVIIAPVVSQAARDVVAKKT
HHHHHHHCCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NVRLLECGQWTAQTKGLDYKRVNGGLLIQDRDQGMVTEAELKVVTKRVPTEAELKDLMFC
CEEEEECCCCCHHCCCCCEEECCCCEEEEECCCCCEEHHHHHHHHHCCCCHHHHHHHHHH
WKVAKFVKSNAIVYAKEGMTIGVGAGQMSRVYSAKIAGIKAADEGLVVEGSVMASDAFFP
HHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCEEECCCCEEEECCEEECCCCCC
FRDGIDAAAAAGISCIIQPGGSIRDEEVIAAADEHGMAMVFTNMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC
>Mature Secondary Structure
MNNARPIRRALLSVSDKTGILEFAQALHAQGVELLSTGGTAKLLADNGVPVIEVSDYTGH
CCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCEEEECCCCEEEEECCCCCEEEECCCCCC
PEIMDGRVKTLHPKVHGGILARRGQDEDVMAANNIGPIDLVAVNLYPFAATVAKPGCTLE
CCCCCCCEEEECCHHHCCEEECCCCCCCEEEECCCCCEEEEEEEEEEHHHHHCCCCCCHH
DAIENIDIGGPTMVRAAAKNHKDVVIVVNAKDYDRVLAEMSANGGSTSHATRFDLAIAAF
HHHHHCCCCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEEEEHH
EHTAAYDGMIANYFGTMVPAHSSDECHDDSKFPRTFNTQLVKKQDLRYGENSHQSAAFYV
HHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEE
DLNSDEASVATATQLQGKALSYNNIADTDAALECVKEFDAPACVIVKHANPCGVALGDNL
EECCCCCHHEEHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEECCHHH
LDAYNRAYKTDPTSAFGGIIAFNRELDGETAAAIVERQFVEVIIAPVVSQAARDVVAKKT
HHHHHHHCCCCCCHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NVRLLECGQWTAQTKGLDYKRVNGGLLIQDRDQGMVTEAELKVVTKRVPTEAELKDLMFC
CEEEEECCCCCHHCCCCCEEECCCCEEEEECCCCCEEHHHHHHHHHCCCCHHHHHHHHHH
WKVAKFVKSNAIVYAKEGMTIGVGAGQMSRVYSAKIAGIKAADEGLVVEGSVMASDAFFP
HHHHHHHHCCCEEEEECCCEEEECCCHHHHHHHHHHCCEEECCCCEEEECCEEECCCCCC
FRDGIDAAAAAGISCIIQPGGSIRDEEVIAAADEHGMAMVFTNMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA