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The map label for this gene is ampE [H]

Identifier: 119773514

GI number: 119773514

Start: 451931

End: 452791

Strand: Direct

Name: ampE [H]

Synonym: Sama_0373

Alternate gene names: 119773514

Gene position: 451931-452791 (Clockwise)

Preceding gene: 119773513

Following gene: 119773515

Centisome position: 10.5

GC content: 51.34

Gene sequence:

>861_bases
ATGGCACTATTTTCATTGTTGATGGCGATACTAGCCGAACGCCTTAAGTTTTTACCCGAGACCTGGCAATTCGACACCCT
GATATCAAAATTGCATCGCCGGCTGTGGAAAGATGAATTTACTCAGGGTCAAGCGTCTGAGTTTCAGGTGTTGTTTGCCC
TGCTGTTACCCGCGGTGTTGGTATATCTGGCACTTATAGTGGTCAGTGGGATTGCCTGGGGAATGCTCACACTGGCGGTG
TGGGTGCTCACCGCTATGGTGTGTTTCAGTCATCAGGAACAACGTAAAGCCTTTAAGCGGTATATTCAGGCGGCATGCAG
GGGCGATACCCAAGCCTGTTATCGTCATGGGTCAGAACTCGATTGCTCTTCCTGTCTTGAAGCCGTGACTGCCGATGAGC
TGGGGCTAAAGGTTGGACAAAGTGTTGCCTGGATAAACTATCGCTTTTACGGCGCTGTGGCCCTGTATTTGATAGCCCTT
GGCCCTGTTGCGGCGGTGCTCTACTGCAGCGTGCGTTACTACGAGCAGATGCAAAAGCGCAGTAATCTGCAGCTCCCTTA
TGTGGATGCGCTACTGACAGTATTGGATTGGCTGCCAAGCCGCATCTTTGCATTTGGTTTTGTGCTGGCAGGACAGTTCA
GCAGCGCATTCAATCGTTGGCGCAAGCTGGCATTCGACCCATCTACTCCCGCCAGACAAATTGTTACGGAAACCGCGACC
GAGGCTGAATCTATTCCACAGCCCTCATCAGCACCTGTATGCTTGCAACCCACATTAGTACTGCTCGGGCTAAGTAAGCG
CAATTTTATACTGCTGCTGGCAGCGGTGTCCCTGCTGACCATTTTTGGGATCATCAACTGA

Upstream 100 bases:

>100_bases
TATCTCAAGAGTTCAGTGGGTAAGAAGTGAGTGGTTTTAAGCTTGCGCTTACCGCACATGGATAAGCCCGGCACGGTGTA
ACGTTAATGGGAGAACCAGG

Downstream 100 bases:

>100_bases
AGTCAATGTGTTGGCTGAAACTACCTTAAATGCAGTACTTGCGTAGGGTTTGTTAATTGGTCTGACCCCATGACAATTTT
TTTCAGCTATGCTCTCATGT

Product: regulatory protein AmpE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MALFSLLMAILAERLKFLPETWQFDTLISKLHRRLWKDEFTQGQASEFQVLFALLLPAVLVYLALIVVSGIAWGMLTLAV
WVLTAMVCFSHQEQRKAFKRYIQAACRGDTQACYRHGSELDCSSCLEAVTADELGLKVGQSVAWINYRFYGAVALYLIAL
GPVAAVLYCSVRYYEQMQKRSNLQLPYVDALLTVLDWLPSRIFAFGFVLAGQFSSAFNRWRKLAFDPSTPARQIVTETAT
EAESIPQPSSAPVCLQPTLVLLGLSKRNFILLLAAVSLLTIFGIIN

Sequences:

>Translated_286_residues
MALFSLLMAILAERLKFLPETWQFDTLISKLHRRLWKDEFTQGQASEFQVLFALLLPAVLVYLALIVVSGIAWGMLTLAV
WVLTAMVCFSHQEQRKAFKRYIQAACRGDTQACYRHGSELDCSSCLEAVTADELGLKVGQSVAWINYRFYGAVALYLIAL
GPVAAVLYCSVRYYEQMQKRSNLQLPYVDALLTVLDWLPSRIFAFGFVLAGQFSSAFNRWRKLAFDPSTPARQIVTETAT
EAESIPQPSSAPVCLQPTLVLLGLSKRNFILLLAAVSLLTIFGIIN
>Mature_285_residues
ALFSLLMAILAERLKFLPETWQFDTLISKLHRRLWKDEFTQGQASEFQVLFALLLPAVLVYLALIVVSGIAWGMLTLAVW
VLTAMVCFSHQEQRKAFKRYIQAACRGDTQACYRHGSELDCSSCLEAVTADELGLKVGQSVAWINYRFYGAVALYLIALG
PVAAVLYCSVRYYEQMQKRSNLQLPYVDALLTVLDWLPSRIFAFGFVLAGQFSSAFNRWRKLAFDPSTPARQIVTETATE
AESIPQPSSAPVCLQPTLVLLGLSKRNFILLLAAVSLLTIFGIIN

Specific function: Putative signaling protein in beta-lactamase regulation. AmpE seems not to act as a direct sensor for beta-lactams [H]

COG id: COG3725

COG function: function code V; Membrane protein required for beta-lactamase induction

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786301, Length=300, Percent_Identity=27, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: NA

Molecular weight: Translated: 32002; Mature: 31871

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCCCCEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALFSLLMAILAERLKFLPETWQFDTLISKLHRRLWKDEFTQGQASEFQVLFALLLPAVL
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VYLALIVVSGIAWGMLTLAVWVLTAMVCFSHQEQRKAFKRYIQAACRGDTQACYRHGSEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
DCSSCLEAVTADELGLKVGQSVAWINYRFYGAVALYLIALGPVAAVLYCSVRYYEQMQKR
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SNLQLPYVDALLTVLDWLPSRIFAFGFVLAGQFSSAFNRWRKLAFDPSTPARQIVTETAT
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
EAESIPQPSSAPVCLQPTLVLLGLSKRNFILLLAAVSLLTIFGIIN
HHHCCCCCCCCCEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503 [H]