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The map label for this gene is ampD [H]

Identifier: 119773513

GI number: 119773513

Start: 451281

End: 451853

Strand: Direct

Name: ampD [H]

Synonym: Sama_0372

Alternate gene names: 119773513

Gene position: 451281-451853 (Clockwise)

Preceding gene: 119773503

Following gene: 119773514

Centisome position: 10.48

GC content: 52.18

Gene sequence:

>573_bases
ATGTCGTTGCAGCCTTTAATACAGTGGCATCAAGGGTGGATAAACCGCGCCCGACATAGCCAGTCACCCTTCTTTAATTC
CCGTCCTCAAGGGGAGATAAGTCTGCTGGTTATTCACAATATCAGTCTGCCCGCAGGCCAGTTTGGCTTACCTTATATAG
AAGCGCTGTTTCAGGGCTGCTTAGACTGCGATGCACACCCCAGTTTTGCGGATTTGCAGGGGCTTGAAGTCTCCGCTCAC
TTTCTTATCCGCCGAAATGGCGAGCTGGTACAATTTGTGTCCTGTGAAGACAGAGCCTGGCATGCTGGCGTGTCCAAGTT
CAATGGCCGTGAAGGTTGCAACGATTTTGCGGTCGGGATTGAGCTTGAGGGGACGGACGACCTTCCCTTTACCGATCTCC
AATATCAGGTACTGCGTGAGCTGACACTGGCTCTGATGGCCGAGTATCCCGATATCACCCAAGACCGAATAGTTGGTCAC
AGTGACATTGCTCCGGGGCGTAAGACCGACCCGGGCCCCAAGTTTGACTGGGCGAGGTATTTTAGGGAATTATCTCAAGA
GTTCAGTGGGTAA

Upstream 100 bases:

>100_bases
CCTTGGTAAAAGCAACGCCGCCAGCGCTCTTGGGCTTTGTACATAGCCGCTGAGAAGAGGTGGATACTGAAAGTCGTCTT
ATTGTGAATACGGAAACCAC

Downstream 100 bases:

>100_bases
GAAGTGAGTGGTTTTAAGCTTGCGCTTACCGCACATGGATAAGCCCGGCACGGTGTAACGTTAATGGGAGAACCAGGATG
GCACTATTTTCATTGTTGAT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MSLQPLIQWHQGWINRARHSQSPFFNSRPQGEISLLVIHNISLPAGQFGLPYIEALFQGCLDCDAHPSFADLQGLEVSAH
FLIRRNGELVQFVSCEDRAWHAGVSKFNGREGCNDFAVGIELEGTDDLPFTDLQYQVLRELTLALMAEYPDITQDRIVGH
SDIAPGRKTDPGPKFDWARYFRELSQEFSG

Sequences:

>Translated_190_residues
MSLQPLIQWHQGWINRARHSQSPFFNSRPQGEISLLVIHNISLPAGQFGLPYIEALFQGCLDCDAHPSFADLQGLEVSAH
FLIRRNGELVQFVSCEDRAWHAGVSKFNGREGCNDFAVGIELEGTDDLPFTDLQYQVLRELTLALMAEYPDITQDRIVGH
SDIAPGRKTDPGPKFDWARYFRELSQEFSG
>Mature_189_residues
SLQPLIQWHQGWINRARHSQSPFFNSRPQGEISLLVIHNISLPAGQFGLPYIEALFQGCLDCDAHPSFADLQGLEVSAHF
LIRRNGELVQFVSCEDRAWHAGVSKFNGREGCNDFAVGIELEGTDDLPFTDLQYQVLRELTLALMAEYPDITQDRIVGHS
DIAPGRKTDPGPKFDWARYFRELSQEFSG

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=180, Percent_Identity=58.3333333333333, Blast_Score=213, Evalue=7e-57,
Organism=Escherichia coli, GI1787092, Length=148, Percent_Identity=36.4864864864865, Blast_Score=81, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21462; Mature: 21331

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQPLIQWHQGWINRARHSQSPFFNSRPQGEISLLVIHNISLPAGQFGLPYIEALFQGC
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHH
LDCDAHPSFADLQGLEVSAHFLIRRNGELVQFVSCEDRAWHAGVSKFNGREGCNDFAVGI
HCCCCCCCHHHCCCCEEEEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCCCCEEEEE
ELEGTDDLPFTDLQYQVLRELTLALMAEYPDITQDRIVGHSDIAPGRKTDPGPKFDWARY
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHH
FRELSQEFSG
HHHHHHHHCC
>Mature Secondary Structure 
SLQPLIQWHQGWINRARHSQSPFFNSRPQGEISLLVIHNISLPAGQFGLPYIEALFQGC
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHH
LDCDAHPSFADLQGLEVSAHFLIRRNGELVQFVSCEDRAWHAGVSKFNGREGCNDFAVGI
HCCCCCCCHHHCCCCEEEEEEEEECCCCEEEEECCCCCHHHHHHHHCCCCCCCCCEEEEE
ELEGTDDLPFTDLQYQVLRELTLALMAEYPDITQDRIVGHSDIAPGRKTDPGPKFDWARY
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHH
FRELSQEFSG
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503; 7959070 [H]