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The map label for this gene is pyrE [H]

Identifier: 119773462

GI number: 119773462

Start: 388412

End: 389053

Strand: Direct

Name: pyrE [H]

Synonym: Sama_0321

Alternate gene names: 119773462

Gene position: 388412-389053 (Clockwise)

Preceding gene: 119773461

Following gene: 119773468

Centisome position: 9.02

GC content: 54.05

Gene sequence:

>642_bases
GTGAAAGCCTATCAGCGCGAGTTTATTGAGTTTGCTCTCGAGCGTAATGTACTGAGATTCGGTGAGTTTACACTCAAATC
CGGCCGCAAGAGCCCTTACTTCTTCAACGCCGGTTTATTTAATACCGGCCGCGATCTGGCCCGTCTCGGCCGCTTCTATG
CCGCTGCGCTGGTGGATGCGGGTATCGACTACGATTTGCTGTTTGGCCCCGCTTATAAAGGCATTCCCATTGCCACCACC
ACGGCGGTAGCCCTGGCCGAGCACCATGATATCGACATGCCTTACTGCTTTAACCGTAAAGAGAAAAAAGATCACGGTGA
AGGTGGTAACCTGGTGGGCAGCGAGCTCAAGGGTAAGGTTATGTTGGTGGATGATGTGATCACCGCCGGTACCGCCATTC
GTGAATCGATGGAAATTATTCAGGCCAATGGTGCCGAGCTGGCCGGGGTGTTGATTGCGCTGGACCGTCAGGAAAAGGGT
AAGGGCGAGCTGTCTGCCATTCAGGAAGTGGAGCGTGACTTTGGCTGCGGTATCGTGGCTATCATCAAGCTGGCCGATTT
GATTGCCTTCCTGGCCGAGAAGCCAGGCATGGAAGAGGTGCTGAGCTCGGTACGTGCTTACCGCGAGCAATACGGCATTT
AA

Upstream 100 bases:

>100_bases
AATTGATTTTTTTAAAGGCTCCGAGAGGGGCCTTTTTACCACCCTGCACCCTGCTTTAATCGGCTGCCTGAGCAGCCAGA
CACATGATAGAGGAAAGACA

Downstream 100 bases:

>100_bases
GCCCAAGCCATAAAAGGTAAGCGCCCGCCAGTTTGGGCCCTTGGCTTAAGCATAAAAAACGCCGCAATCAGCGGCGTTTT
TTATTGGGAATACGCTTAAG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKAYQREFIEFALERNVLRFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALVDAGIDYDLLFGPAYKGIPIATT
TAVALAEHHDIDMPYCFNRKEKKDHGEGGNLVGSELKGKVMLVDDVITAGTAIRESMEIIQANGAELAGVLIALDRQEKG
KGELSAIQEVERDFGCGIVAIIKLADLIAFLAEKPGMEEVLSSVRAYREQYGI

Sequences:

>Translated_213_residues
MKAYQREFIEFALERNVLRFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALVDAGIDYDLLFGPAYKGIPIATT
TAVALAEHHDIDMPYCFNRKEKKDHGEGGNLVGSELKGKVMLVDDVITAGTAIRESMEIIQANGAELAGVLIALDRQEKG
KGELSAIQEVERDFGCGIVAIIKLADLIAFLAEKPGMEEVLSSVRAYREQYGI
>Mature_213_residues
MKAYQREFIEFALERNVLRFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALVDAGIDYDLLFGPAYKGIPIATT
TAVALAEHHDIDMPYCFNRKEKKDHGEGGNLVGSELKGKVMLVDDVITAGTAIRESMEIIQANGAELAGVLIALDRQEKG
KGELSAIQEVERDFGCGIVAIIKLADLIAFLAEKPGMEEVLSSVRAYREQYGI

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=150, Percent_Identity=30, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=80.7511737089202, Blast_Score=366, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17508631, Length=163, Percent_Identity=28.2208588957055, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323530, Length=220, Percent_Identity=47.2727272727273, Blast_Score=197, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6323927, Length=221, Percent_Identity=43.8914027149321, Blast_Score=187, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23431; Mature: 23431

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAYQREFIEFALERNVLRFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALVDA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GIDYDLLFGPAYKGIPIATTTAVALAEHHDIDMPYCFNRKEKKDHGEGGNLVGSELKGKV
CCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCE
MLVDDVITAGTAIRESMEIIQANGAELAGVLIALDRQEKGKGELSAIQEVERDFGCGIVA
EEEHHHHHHHHHHHHHHHHHHCCCCHHEEHEEEECCCCCCCCHHHHHHHHHHHHCCHHHH
IIKLADLIAFLAEKPGMEEVLSSVRAYREQYGI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKAYQREFIEFALERNVLRFGEFTLKSGRKSPYFFNAGLFNTGRDLARLGRFYAAALVDA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GIDYDLLFGPAYKGIPIATTTAVALAEHHDIDMPYCFNRKEKKDHGEGGNLVGSELKGKV
CCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCE
MLVDDVITAGTAIRESMEIIQANGAELAGVLIALDRQEKGKGELSAIQEVERDFGCGIVA
EEEHHHHHHHHHHHHHHHHHHCCCCHHEEHEEEECCCCCCCCHHHHHHHHHHHHCCHHHH
IIKLADLIAFLAEKPGMEEVLSSVRAYREQYGI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]