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The map label for this gene is rph

Identifier: 119773461

GI number: 119773461

Start: 387592

End: 388305

Strand: Direct

Name: rph

Synonym: Sama_0320

Alternate gene names: 119773461

Gene position: 387592-388305 (Clockwise)

Preceding gene: 119773448

Following gene: 119773462

Centisome position: 9.0

GC content: 56.44

Gene sequence:

>714_bases
ATGCGTCCAGGTAATCGTACCCCGGCTCAGACCCGCCCCATCAGCATCACCCGTAACTTTACTGCCCACGCCGAAGGCTC
TGTGCTGGTAGAGTTTGGCGAAACCAAGGTGCTGTGTACCGCCAGTTTCACCGAGGGTGTACCACGCTTCCTTAAAGGCA
GTGGGCAGGGTTGGTTGACTGCCGAATATGGCATGCTGCCGCGTTCTACCCATAGCCGTATGGACCGCGAAGCCGCCCGT
GGCAAACAGTCTGGCCGTACCCAGGAAATCCAGCGTCTGATTGGCCGCGCCCTGCGTGCTGCCGTGGATTTAAAGCAACT
GGGCGAAAACACCATAGTGGTGGACTGTGATGTTATTCAGGCCGATGGTGGCACCCGTACCGCAGCTATCACAGGAGCCT
GTGTGGCACTGGTGGATGCGCTGAACTGGGCCCGCGGTAAAGGTTTGGTGAAAGCCAACCCACTGAAGTTTTTGATTGCG
GCTGTGAGCGTGGGTATCCACAACGGCGAAGCCATTTGCGATCTCGAGTATGTGGAAGACAGCGCTGCCGAAACCGATAT
GAATGTGGTGATGACCGAAACCGGCAAGATCATTGAAGTTCAGGGTACGGCCGAGGGTGAGCCTTTCACCCACGAAGAGT
TGCTGACCATGCTGGAGCTGGCCAAACACGGTATCCGCGAAATCATTGATACTCAGAAAGCGGCTCTGAACTGA

Upstream 100 bases:

>100_bases
CGCGCGGAGTGTATTCAAAATCCACCGTGGCAACTCGCTCTGCATAGCCCTATAATAGGCAGCCAAATTATTGCGAAATT
CATACGTCACAGGAATCGCC

Downstream 100 bases:

>100_bases
TTACAGAATTGATTTTTTTAAAGGCTCCGAGAGGGGCCTTTTTACCACCCTGCACCCTGCTTTAATCGGCTGCCTGAGCA
GCCAGACACATGATAGAGGA

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRPGNRTPAQTRPISITRNFTAHAEGSVLVEFGETKVLCTASFTEGVPRFLKGSGQGWLTAEYGMLPRSTHSRMDREAAR
GKQSGRTQEIQRLIGRALRAAVDLKQLGENTIVVDCDVIQADGGTRTAAITGACVALVDALNWARGKGLVKANPLKFLIA
AVSVGIHNGEAICDLEYVEDSAAETDMNVVMTETGKIIEVQGTAEGEPFTHEELLTMLELAKHGIREIIDTQKAALN

Sequences:

>Translated_237_residues
MRPGNRTPAQTRPISITRNFTAHAEGSVLVEFGETKVLCTASFTEGVPRFLKGSGQGWLTAEYGMLPRSTHSRMDREAAR
GKQSGRTQEIQRLIGRALRAAVDLKQLGENTIVVDCDVIQADGGTRTAAITGACVALVDALNWARGKGLVKANPLKFLIA
AVSVGIHNGEAICDLEYVEDSAAETDMNVVMTETGKIIEVQGTAEGEPFTHEELLTMLELAKHGIREIIDTQKAALN
>Mature_237_residues
MRPGNRTPAQTRPISITRNFTAHAEGSVLVEFGETKVLCTASFTEGVPRFLKGSGQGWLTAEYGMLPRSTHSRMDREAAR
GKQSGRTQEIQRLIGRALRAAVDLKQLGENTIVVDCDVIQADGGTRTAAITGACVALVDALNWARGKGLVKANPLKFLIA
AVSVGIHNGEAICDLEYVEDSAAETDMNVVMTETGKIIEVQGTAEGEPFTHEELLTMLELAKHGIREIIDTQKAALN

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=78.1990521327014, Blast_Score=343, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI71981632, Length=187, Percent_Identity=28.3422459893048, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_SHEAM (A1S2C5)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926201.1
- ProteinModelPortal:   A1S2C5
- SMR:   A1S2C5
- STRING:   A1S2C5
- GeneID:   4602576
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0320
- NMPDR:   fig|326297.7.peg.305
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   A1S2C5
- ProtClustDB:   PRK00173
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25529; Mature: 25529

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPGNRTPAQTRPISITRNFTAHAEGSVLVEFGETKVLCTASFTEGVPRFLKGSGQGWLT
CCCCCCCCCCCCCEEEEECEEECCCCCEEEEECCEEEEEEECHHHCCHHHHCCCCCCEEE
AEYGMLPRSTHSRMDREAARGKQSGRTQEIQRLIGRALRAAVDLKQLGENTIVVDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTAAITGACVALVDALNWARGKGLVKANPLKFLIAAVSVGIHNGEAICDLEYVED
CCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCEEEEEHHHCC
SAAETDMNVVMTETGKIIEVQGTAEGEPFTHEELLTMLELAKHGIREIIDTQKAALN
CCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPGNRTPAQTRPISITRNFTAHAEGSVLVEFGETKVLCTASFTEGVPRFLKGSGQGWLT
CCCCCCCCCCCCCEEEEECEEECCCCCEEEEECCEEEEEEECHHHCCHHHHCCCCCCEEE
AEYGMLPRSTHSRMDREAARGKQSGRTQEIQRLIGRALRAAVDLKQLGENTIVVDCDVIQ
EECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTAAITGACVALVDALNWARGKGLVKANPLKFLIAAVSVGIHNGEAICDLEYVED
CCCCCEEHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCEEEEEHHHCC
SAAETDMNVVMTETGKIIEVQGTAEGEPFTHEELLTMLELAKHGIREIIDTQKAALN
CCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA