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The map label for this gene is ebgA [C]

Identifier: 119773443

GI number: 119773443

Start: 365361

End: 367952

Strand: Direct

Name: ebgA [C]

Synonym: Sama_0302

Alternate gene names: 119773443

Gene position: 365361-367952 (Clockwise)

Preceding gene: 119773442

Following gene: 119773444

Centisome position: 8.48

GC content: 55.44

Gene sequence:

>2592_bases
ATGACAACACATTCCTTCGAGCCTTTTGGTCAATCTCTGAACTCTTTGGGGCAATCAATGGACCTGGGCGGCCAGTGGCA
ATTTCGTCAGCAGGGTAAAGCCGAATGGCTTCCGGCTCAGGTGCCCGGCTGTAACTTTACCGACCTGCTCGCCAATGGTC
TGATTGACGACCCCTTCTATCGGGACAATGAAGAAAAGCTGCAATGGATTGAGCAAGAGGACTGGCAGTATCGCAAGACC
TTTAGTCTGGATACTGACTGGTGGCAAAACGATGAGGTGCAACTGGTCGCCGATGGCCTGGATACCTATTGCGACCTGTA
CCTCAATGGCCATAAGCTTGGCGACAGTCGCAACATGTTTATTGGCCAGCGTATTTCCTGTAAGTCGCTGCTGAAGGTCG
GGGAAAACCTGCTGGAGATTCATTTTCGTTCACCCATTAAGGAAACCCTGCCGCTGCATCAGGCGGCGGGCTTTACCTAT
CCGGCGGAAAACGACAAGTCGGTCGAGAAACTCAGTGTGTACAATCGCAAGGCGCCCTGCCATTTTGGCTGGGACTGGGG
CCCAAGGTTTGTCACCAGTGGCATCTGGCGTGCCATCGGTCTGCAGGGAATCAAACGAGGCCGTATTGATGGGGTGCATT
TTGTGCAGCATAGCCTCAGCAGTGACAAGGCTGAGTTCAGCTTCGATGTGAGCCTGGCGCTGTGCAATACAGACAATCAG
GAAGGCGAGCTGAGTCTGCGGGTTGCGTGCCCGGCTGCGCCCGAGCTTAACCGGGAAGTGCCACTTAACCGTGACGTGCC
GCTGACTGAACAATCCGCGCTGCGATTGGACTTCGAGCTCACCAATCCCAATCTCTGGTGGCCCAATGGTCTGGGTGAAG
CCCACCTATATGAGTTCCATTTTACCTTGCTCGCCGGCGATGAGCCGTTGGCACAGCACAGCCAGGCCGTTGGTCTGCGT
ACCCTGGAAGTCATCAACGAAGCTGATGGCATGGGGCAATCCTTCTACCTCAGGGTCAATGGCCAGCCGGTGTTTATGAA
GGGGGCCAACTATATTCCCGGTGACAGCTTTATCCACCGCATGACACCCGATCGTCATCAGGCTGACTTTGACGCCGTGG
TGGATGCCAACATGAATATGCTGCGGGTATGGGGCGGTGGTGTGTATCAGGATGAGGTCTTTTATCGCCTGGCCGATGAA
AACGGCATCCTGATTTGGCAGGATTTTATGTTCGCCTGCTCCCTGTACCCGGCCGATGAAGCCTTTCTGGACAACGTCCG
CGAAGAAGCGGTTTATAACATCAAGCGCCTGCGCAATCATCCCTGTTTGGCACTCTGGTGCGGCAACAACGAAGTGGACA
TGGGTATCAAGCATTGGCAGTGGCCTGAGAAGTTTGGCTACAGCGATGAACTCTATGCCCGCCTGAAGAACGACTACATC
CGCTTGTTCGATGAGTGTCTGCCTGGGGCCGTGGCCGAGTTGGACGCCGGACGCTTTTATTTGCGCTCGTCCCCCATAGG
CTTCTGGGAAGAAGACATGGACCACATAGGCAATCACCATTACTGGGGGGTGTGGCACGGCGAGGAGCCCTTCAGCGAAT
ATCAGAAACGTATTCCCCGCTTTATGAGTGAGTTCGGCTTTCAGTCGTTCCCGCTGGCCTCGTCCATGGCGCGTTTTACC
GAGGAGACTGACTGGCATCTGGAATCAAGCGTGCTCAGGGTGCATCAGAAACACCCCCGGGGTAACAAGCTTATTCGCAG
CTACATGGAGCAGGAATACCGGGATCCGGAAAACTTCCCACGACTGTTGTTTTTAAGTCAGGTACAACAGGCAGAAGGCC
TGAAGCTGGCGTTTGAGGCCCACAGGGCTGCGCGGCCATTTTGCATGGGCAGCCTCTACTGGCAGCTTAACGACACCTGG
CCCGCGGCGTCCTGGTCCGGTATCGATTATTACGGCCGCTGGAAGGCGCTGCACTATCAGGCCAAACGCAGTTTCCGCAC
CGACCTGCTGGTGGTGGATGAGGTCGATGGCGCTGTTCGTGTCCGTTTGGTGAGCGACCGCCTGAAGTCGGTTAAGGCAA
GGCTCGTGCTTGAGCTGCTGGACTTTGATGGCAACTGCCTGTGGCAGGAGCAGCAAGCGCTTGATACCCCAAAAAATACC
AGCACCCAGGTATGCCAGTTGGAGAGGGCAAGCCTGCTTGGCGAGCACAATCCCTGTCAGCTGATGCTGAGGGGACGCCT
GCTGGATGAGCAGGGGCAACTGCTGACCGATTGCCTGTATTATTTTGTGCCGAGCAAAGATCTGGCACTGAAAACGCCGG
CGCTGACCCTTGAGCCAAGCTTCGATGCTGAGGGGCTCCGCCTGACCCTTAACACCAATACGCTTATTCGCCAGTGCCAT
TTGGAGCTGCCTGAAGTTGCCGGTAATTGCTCTGGTCATTGCTCTGGTAATTTTTCCGACAACTTCTTCGATTTGGTCCC
CGGCGAGCCAAAGTGCATCCACATTGCTTTACTGGGCATGGATGAGGATACCAAGGCGGCTCTGGTGAAGGGCATTCGCT
GTCAATCCCTGGTGCAGGAGGAAACCCAATGA

Upstream 100 bases:

>100_bases
GCGTGAGCCAGGCAAAGGTGGCTGAGCTGGTAGCCAATATTCAGGCCAATACCTTATTACAAGTGCCGCAAGGGCGGACT
TTTACATGTTGAAGAGCATT

Downstream 100 bases:

>100_bases
CCCTGGCATGGGTGGATTTACTGGTTATAGGCGCGTATCTGCTGGGCACGTTGGGGCTTGGGGTTTATATCTCCCGCCTT
GCCAGCAGAGACATGAATGC

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Glycoside Hydrolase Family Protein; Beta-Mannosidase Protein; Glycoside Hydrolase; Mannosidase; Coagulation Factor 5/8 Type Domain Protein; Glycoside Hydrolase Family 2 Protein; Glycosidase; O-Glycosyl Hydrolase; Glycosyl Hydrolase; Glycoside Hydrolase Family 2 TIM Barrel; Beta-Galactosidase/Beta-Glucuronidase; Beta-Glucuronidase; Exported Glycosyl Hydrolase; Beta-Galactosidase/Beta- Glucuronidase Family Protein; Beta-Galactosidase/Beta; Exported Beta-Mannosidase-Like Glycosidase; Beta-D-Mannosidase; Mannosylglycoprotein Endo-Beta-Mannosidase; Beta-Mannosidase-Related Protein; Beta-Mannosidase Man2A; BETA-Mannosidase Protein; DS Domain/Family 2 Glycosyl Hydrolase

Number of amino acids: Translated: 863; Mature: 862

Protein sequence:

>863_residues
MTTHSFEPFGQSLNSLGQSMDLGGQWQFRQQGKAEWLPAQVPGCNFTDLLANGLIDDPFYRDNEEKLQWIEQEDWQYRKT
FSLDTDWWQNDEVQLVADGLDTYCDLYLNGHKLGDSRNMFIGQRISCKSLLKVGENLLEIHFRSPIKETLPLHQAAGFTY
PAENDKSVEKLSVYNRKAPCHFGWDWGPRFVTSGIWRAIGLQGIKRGRIDGVHFVQHSLSSDKAEFSFDVSLALCNTDNQ
EGELSLRVACPAAPELNREVPLNRDVPLTEQSALRLDFELTNPNLWWPNGLGEAHLYEFHFTLLAGDEPLAQHSQAVGLR
TLEVINEADGMGQSFYLRVNGQPVFMKGANYIPGDSFIHRMTPDRHQADFDAVVDANMNMLRVWGGGVYQDEVFYRLADE
NGILIWQDFMFACSLYPADEAFLDNVREEAVYNIKRLRNHPCLALWCGNNEVDMGIKHWQWPEKFGYSDELYARLKNDYI
RLFDECLPGAVAELDAGRFYLRSSPIGFWEEDMDHIGNHHYWGVWHGEEPFSEYQKRIPRFMSEFGFQSFPLASSMARFT
EETDWHLESSVLRVHQKHPRGNKLIRSYMEQEYRDPENFPRLLFLSQVQQAEGLKLAFEAHRAARPFCMGSLYWQLNDTW
PAASWSGIDYYGRWKALHYQAKRSFRTDLLVVDEVDGAVRVRLVSDRLKSVKARLVLELLDFDGNCLWQEQQALDTPKNT
STQVCQLERASLLGEHNPCQLMLRGRLLDEQGQLLTDCLYYFVPSKDLALKTPALTLEPSFDAEGLRLTLNTNTLIRQCH
LELPEVAGNCSGHCSGNFSDNFFDLVPGEPKCIHIALLGMDEDTKAALVKGIRCQSLVQEETQ

Sequences:

>Translated_863_residues
MTTHSFEPFGQSLNSLGQSMDLGGQWQFRQQGKAEWLPAQVPGCNFTDLLANGLIDDPFYRDNEEKLQWIEQEDWQYRKT
FSLDTDWWQNDEVQLVADGLDTYCDLYLNGHKLGDSRNMFIGQRISCKSLLKVGENLLEIHFRSPIKETLPLHQAAGFTY
PAENDKSVEKLSVYNRKAPCHFGWDWGPRFVTSGIWRAIGLQGIKRGRIDGVHFVQHSLSSDKAEFSFDVSLALCNTDNQ
EGELSLRVACPAAPELNREVPLNRDVPLTEQSALRLDFELTNPNLWWPNGLGEAHLYEFHFTLLAGDEPLAQHSQAVGLR
TLEVINEADGMGQSFYLRVNGQPVFMKGANYIPGDSFIHRMTPDRHQADFDAVVDANMNMLRVWGGGVYQDEVFYRLADE
NGILIWQDFMFACSLYPADEAFLDNVREEAVYNIKRLRNHPCLALWCGNNEVDMGIKHWQWPEKFGYSDELYARLKNDYI
RLFDECLPGAVAELDAGRFYLRSSPIGFWEEDMDHIGNHHYWGVWHGEEPFSEYQKRIPRFMSEFGFQSFPLASSMARFT
EETDWHLESSVLRVHQKHPRGNKLIRSYMEQEYRDPENFPRLLFLSQVQQAEGLKLAFEAHRAARPFCMGSLYWQLNDTW
PAASWSGIDYYGRWKALHYQAKRSFRTDLLVVDEVDGAVRVRLVSDRLKSVKARLVLELLDFDGNCLWQEQQALDTPKNT
STQVCQLERASLLGEHNPCQLMLRGRLLDEQGQLLTDCLYYFVPSKDLALKTPALTLEPSFDAEGLRLTLNTNTLIRQCH
LELPEVAGNCSGHCSGNFSDNFFDLVPGEPKCIHIALLGMDEDTKAALVKGIRCQSLVQEETQ
>Mature_862_residues
TTHSFEPFGQSLNSLGQSMDLGGQWQFRQQGKAEWLPAQVPGCNFTDLLANGLIDDPFYRDNEEKLQWIEQEDWQYRKTF
SLDTDWWQNDEVQLVADGLDTYCDLYLNGHKLGDSRNMFIGQRISCKSLLKVGENLLEIHFRSPIKETLPLHQAAGFTYP
AENDKSVEKLSVYNRKAPCHFGWDWGPRFVTSGIWRAIGLQGIKRGRIDGVHFVQHSLSSDKAEFSFDVSLALCNTDNQE
GELSLRVACPAAPELNREVPLNRDVPLTEQSALRLDFELTNPNLWWPNGLGEAHLYEFHFTLLAGDEPLAQHSQAVGLRT
LEVINEADGMGQSFYLRVNGQPVFMKGANYIPGDSFIHRMTPDRHQADFDAVVDANMNMLRVWGGGVYQDEVFYRLADEN
GILIWQDFMFACSLYPADEAFLDNVREEAVYNIKRLRNHPCLALWCGNNEVDMGIKHWQWPEKFGYSDELYARLKNDYIR
LFDECLPGAVAELDAGRFYLRSSPIGFWEEDMDHIGNHHYWGVWHGEEPFSEYQKRIPRFMSEFGFQSFPLASSMARFTE
ETDWHLESSVLRVHQKHPRGNKLIRSYMEQEYRDPENFPRLLFLSQVQQAEGLKLAFEAHRAARPFCMGSLYWQLNDTWP
AASWSGIDYYGRWKALHYQAKRSFRTDLLVVDEVDGAVRVRLVSDRLKSVKARLVLELLDFDGNCLWQEQQALDTPKNTS
TQVCQLERASLLGEHNPCQLMLRGRLLDEQGQLLTDCLYYFVPSKDLALKTPALTLEPSFDAEGLRLTLNTNTLIRQCHL
ELPEVAGNCSGHCSGNFSDNFFDLVPGEPKCIHIALLGMDEDTKAALVKGIRCQSLVQEETQ

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI84798622, Length=703, Percent_Identity=31.7211948790896, Blast_Score=341, Evalue=2e-93,
Organism=Caenorhabditis elegans, GI17550784, Length=725, Percent_Identity=29.5172413793103, Blast_Score=305, Evalue=7e-83,
Organism=Drosophila melanogaster, GI24643838, Length=673, Percent_Identity=32.9866270430906, Blast_Score=303, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24643840, Length=617, Percent_Identity=33.8735818476499, Blast_Score=278, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.2.1.25

Molecular weight: Translated: 98999; Mature: 98867

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTHSFEPFGQSLNSLGQSMDLGGQWQFRQQGKAEWLPAQVPGCNFTDLLANGLIDDPFY
CCCCCCCHHHHHHHHHHHCCCCCCCEEHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC
RDNEEKLQWIEQEDWQYRKTFSLDTDWWQNDEVQLVADGLDTYCDLYLNGHKLGDSRNMF
CCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEECCCCCEEEEEECCEEECCCCCEE
IGQRISCKSLLKVGENLLEIHFRSPIKETLPLHQAAGFTYPAENDKSVEKLSVYNRKAPC
ECCCCCHHHHHHHCCHHEEHHHCCCHHHHCCHHHHCCCCCCCCCCCCHHHHHHHCCCCCC
HFGWDWGPRFVTSGIWRAIGLQGIKRGRIDGVHFVQHSLSSDKAEFSFDVSLALCNTDNQ
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEECCCCC
EGELSLRVACPAAPELNREVPLNRDVPLTEQSALRLDFELTNPNLWWPNGLGEAHLYEFH
CCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEECCCCCCCCEEEEEE
FTLLAGDEPLAQHSQAVGLRTLEVINEADGMGQSFYLRVNGQPVFMKGANYIPGDSFIHR
EEEEECCCCHHHHHHHHCHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCCCHHHCC
MTPDRHQADFDAVVDANMNMLRVWGGGVYQDEVFYRLADENGILIWQDFMFACSLYPADE
CCCCCCCCCCHHEECCCCCEEEEECCCEECCCEEEEEECCCCEEEEECHHHHHCCCCCCH
AFLDNVREEAVYNIKRLRNHPCLALWCGNNEVDMGIKHWQWPEKFGYSDELYARLKNDYI
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHH
RLFDECLPGAVAELDAGRFYLRSSPIGFWEEDMDHIGNHHYWGVWHGEEPFSEYQKRIPR
HHHHHHCCCHHHHCCCCEEEEECCCCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
FMSEFGFQSFPLASSMARFTEETDWHLESSVLRVHQKHPRGNKLIRSYMEQEYRDPENFP
HHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCH
RLLFLSQVQQAEGLKLAFEAHRAARPFCMGSLYWQLNDTWPAASWSGIDYYGRWKALHYQ
HHHHHHHHHHCCCCEEEEHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHH
AKRSFRTDLLVVDEVDGAVRVRLVSDRLKSVKARLVLELLDFDGNCLWQEQQALDTPKNT
HHHCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECHHHHCCCCCCC
STQVCQLERASLLGEHNPCQLMLRGRLLDEQGQLLTDCLYYFVPSKDLALKTPALTLEPS
CHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEECCEEEECCC
FDAEGLRLTLNTNTLIRQCHLELPEVAGNCSGHCSGNFSDNFFDLVPGEPKCIHIALLGM
CCCCCEEEEECCHHHHHHHHCCCHHHCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEEEC
DEDTKAALVKGIRCQSLVQEETQ
CCCHHHHHHHCHHHHHHHHHCCC
>Mature Secondary Structure 
TTHSFEPFGQSLNSLGQSMDLGGQWQFRQQGKAEWLPAQVPGCNFTDLLANGLIDDPFY
CCCCCCHHHHHHHHHHHCCCCCCCEEHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCCCC
RDNEEKLQWIEQEDWQYRKTFSLDTDWWQNDEVQLVADGLDTYCDLYLNGHKLGDSRNMF
CCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEECCCCCEEEEEECCEEECCCCCEE
IGQRISCKSLLKVGENLLEIHFRSPIKETLPLHQAAGFTYPAENDKSVEKLSVYNRKAPC
ECCCCCHHHHHHHCCHHEEHHHCCCHHHHCCHHHHCCCCCCCCCCCCHHHHHHHCCCCCC
HFGWDWGPRFVTSGIWRAIGLQGIKRGRIDGVHFVQHSLSSDKAEFSFDVSLALCNTDNQ
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEECCCCC
EGELSLRVACPAAPELNREVPLNRDVPLTEQSALRLDFELTNPNLWWPNGLGEAHLYEFH
CCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEECCCCCCCCEEEEEE
FTLLAGDEPLAQHSQAVGLRTLEVINEADGMGQSFYLRVNGQPVFMKGANYIPGDSFIHR
EEEEECCCCHHHHHHHHCHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCCCHHHCC
MTPDRHQADFDAVVDANMNMLRVWGGGVYQDEVFYRLADENGILIWQDFMFACSLYPADE
CCCCCCCCCCHHEECCCCCEEEEECCCEECCCEEEEEECCCCEEEEECHHHHHCCCCCCH
AFLDNVREEAVYNIKRLRNHPCLALWCGNNEVDMGIKHWQWPEKFGYSDELYARLKNDYI
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHH
RLFDECLPGAVAELDAGRFYLRSSPIGFWEEDMDHIGNHHYWGVWHGEEPFSEYQKRIPR
HHHHHHCCCHHHHCCCCEEEEECCCCCCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
FMSEFGFQSFPLASSMARFTEETDWHLESSVLRVHQKHPRGNKLIRSYMEQEYRDPENFP
HHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCH
RLLFLSQVQQAEGLKLAFEAHRAARPFCMGSLYWQLNDTWPAASWSGIDYYGRWKALHYQ
HHHHHHHHHHCCCCEEEEHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHH
AKRSFRTDLLVVDEVDGAVRVRLVSDRLKSVKARLVLELLDFDGNCLWQEQQALDTPKNT
HHHCCCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECHHHHCCCCCCC
STQVCQLERASLLGEHNPCQLMLRGRLLDEQGQLLTDCLYYFVPSKDLALKTPALTLEPS
CHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEECCEEEECCC
FDAEGLRLTLNTNTLIRQCHLELPEVAGNCSGHCSGNFSDNFFDLVPGEPKCIHIALLGM
CCCCCEEEEECCHHHHHHHHCCCHHHCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEEEC
DEDTKAALVKGIRCQSLVQEETQ
CCCHHHHHHHCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA