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The map label for this gene is argF [H]

Identifier: 119773399

GI number: 119773399

Start: 295867

End: 296772

Strand: Direct

Name: argF [H]

Synonym: Sama_0258

Alternate gene names: 119773399

Gene position: 295867-296772 (Clockwise)

Preceding gene: 119773398

Following gene: 119773400

Centisome position: 6.87

GC content: 54.86

Gene sequence:

>906_bases
ATGAAGCATTTGATATCGATAAAAGAACTGACGCAAAAGCAGATGTTGGATCTGCTGACTCTGGCAAAGGAGATCAAGGC
CGACCCGGCCAAATACTCCCGCGCACTGGCGGGCAAGAGTGTGGTAATGCTGTTTGAAAAGCCTTCTCTGCGTACCCGCG
TCAGCTTCGATATCGGTATCAACAAGCTCGGTGGTCACTGCCTGTATCTGGACCAGCAAAACGGGGCATTGGGCAAGCGC
GAGTCGGTAAAAGACTTTGCCTCTAACCTGTCTTGCTGGGCCGATGCCATTGTGGCCCGTACCTTCGCCCACGAAACTGT
GGAAGGTCTTGCCAAGTATGGCCGGGTGCCGGTGATCAATGCGCTGTCGGACCTTTACCATCCCTGTCAGGGATTGGCTG
ATTTTCTGAGTCTGGCCGAAAAGTTCGACGACCTGTCGAAAGTGCGTTTGGCCTATGTGGGTGATGGCAACAACGTCAGC
CATTCGCTGATGTACGGCGCCGCCTTGCTCGGGGCCACCATGACGGTGATCTGTCCTCCAGGACACTTCCCTGATGGCTT
TGAGGTGCAGCAGGCGCAGCAAATTGCTGCGGCCCACGGTGGAAAACTGGTGTTAACCTCTGATATCAATGCCATAGAAG
GCCACGATGCCGTCTACACTGATACCTGGATCTCCATGGGCGACAACACGGCGCTGACTGACATCAAGGCCAAATTTGCG
CCTTACCAGGTGAATAAGGCACTGATGAACAAGGCCGGAGCCAGCTATTTTATGCACTGTCTGCCTGCGCATCGGGGGCT
GGAAGTGACCGATGAGGTGATGGACGGTGAAGGTTCCCTCATCCTGGATCAGGCCGAGAACCGCATGCACGCACAAAACG
CAGTGTTGGTCACACTGCTGGGATAA

Upstream 100 bases:

>100_bases
ACAACCGGTACAGGTTGCCTCGTGGCGTGACAGCGGCCAAATGGCTGCGTTAATCAAGGGTGAGGCCGTGGGCACACAGA
TACAACCTCAGGAGTAATAT

Downstream 100 bases:

>100_bases
ATTCAATCTATTGAGTCAACGGCGCCCCACGCGCCTCTTGGGAAACAGTTATGGCAATCGAAAACAGCAAAACCGGCGTC
AAGAAAGTAGTACTGGCCTA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKHLISIKELTQKQMLDLLTLAKEIKADPAKYSRALAGKSVVMLFEKPSLRTRVSFDIGINKLGGHCLYLDQQNGALGKR
ESVKDFASNLSCWADAIVARTFAHETVEGLAKYGRVPVINALSDLYHPCQGLADFLSLAEKFDDLSKVRLAYVGDGNNVS
HSLMYGAALLGATMTVICPPGHFPDGFEVQQAQQIAAAHGGKLVLTSDINAIEGHDAVYTDTWISMGDNTALTDIKAKFA
PYQVNKALMNKAGASYFMHCLPAHRGLEVTDEVMDGEGSLILDQAENRMHAQNAVLVTLLG

Sequences:

>Translated_301_residues
MKHLISIKELTQKQMLDLLTLAKEIKADPAKYSRALAGKSVVMLFEKPSLRTRVSFDIGINKLGGHCLYLDQQNGALGKR
ESVKDFASNLSCWADAIVARTFAHETVEGLAKYGRVPVINALSDLYHPCQGLADFLSLAEKFDDLSKVRLAYVGDGNNVS
HSLMYGAALLGATMTVICPPGHFPDGFEVQQAQQIAAAHGGKLVLTSDINAIEGHDAVYTDTWISMGDNTALTDIKAKFA
PYQVNKALMNKAGASYFMHCLPAHRGLEVTDEVMDGEGSLILDQAENRMHAQNAVLVTLLG
>Mature_301_residues
MKHLISIKELTQKQMLDLLTLAKEIKADPAKYSRALAGKSVVMLFEKPSLRTRVSFDIGINKLGGHCLYLDQQNGALGKR
ESVKDFASNLSCWADAIVARTFAHETVEGLAKYGRVPVINALSDLYHPCQGLADFLSLAEKFDDLSKVRLAYVGDGNNVS
HSLMYGAALLGATMTVICPPGHFPDGFEVQQAQQIAAAHGGKLVLTSDINAIEGHDAVYTDTWISMGDNTALTDIKAKFA
PYQVNKALMNKAGASYFMHCLPAHRGLEVTDEVMDGEGSLILDQAENRMHAQNAVLVTLLG

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=304, Percent_Identity=40.1315789473684, Blast_Score=211, Evalue=7e-55,
Organism=Homo sapiens, GI18105007, Length=309, Percent_Identity=28.1553398058252, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1790703, Length=326, Percent_Identity=36.5030674846626, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI1786469, Length=327, Percent_Identity=37.0030581039755, Blast_Score=203, Evalue=1e-53,
Organism=Escherichia coli, GI2367364, Length=319, Percent_Identity=29.153605015674, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI193204318, Length=330, Percent_Identity=26.3636363636364, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322373, Length=310, Percent_Identity=32.5806451612903, Blast_Score=172, Evalue=7e-44,
Organism=Saccharomyces cerevisiae, GI6322331, Length=193, Percent_Identity=27.979274611399, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24642586, Length=309, Percent_Identity=25.8899676375405, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 32663; Mature: 32663

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLISIKELTQKQMLDLLTLAKEIKADPAKYSRALAGKSVVMLFEKPSLRTRVSFDIGI
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEECCCCCEEEEEECCC
NKLGGHCLYLDQQNGALGKRESVKDFASNLSCWADAIVARTFAHETVEGLAKYGRVPVIN
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ALSDLYHPCQGLADFLSLAEKFDDLSKVRLAYVGDGNNVSHSLMYGAALLGATMTVICPP
HHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCHHHHHHHHHHHHCEEEEEECC
GHFPDGFEVQQAQQIAAAHGGKLVLTSDINAIEGHDAVYTDTWISMGDNTALTDIKAKFA
CCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEHEEECCCCCEEHHHHHHCC
PYQVNKALMNKAGASYFMHCLPAHRGLEVTDEVMDGEGSLILDQAENRMHAQNAVLVTLL
CHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHCCCCCEEEECHHCHHHHCCEEEEEEE
G
C
>Mature Secondary Structure
MKHLISIKELTQKQMLDLLTLAKEIKADPAKYSRALAGKSVVMLFEKPSLRTRVSFDIGI
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEEECCCCCEEEEEECCC
NKLGGHCLYLDQQNGALGKRESVKDFASNLSCWADAIVARTFAHETVEGLAKYGRVPVIN
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
ALSDLYHPCQGLADFLSLAEKFDDLSKVRLAYVGDGNNVSHSLMYGAALLGATMTVICPP
HHHHHHHHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCCHHHHHHHHHHHHCEEEEEECC
GHFPDGFEVQQAQQIAAAHGGKLVLTSDINAIEGHDAVYTDTWISMGDNTALTDIKAKFA
CCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEHEEECCCCCEEHHHHHHCC
PYQVNKALMNKAGASYFMHCLPAHRGLEVTDEVMDGEGSLILDQAENRMHAQNAVLVTLL
CHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHCCCCCEEEECHHCHHHHCCEEEEEEE
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]