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The map label for this gene is argG

Identifier: 119773400

GI number: 119773400

Start: 296823

End: 298046

Strand: Direct

Name: argG

Synonym: Sama_0259

Alternate gene names: 119773400

Gene position: 296823-298046 (Clockwise)

Preceding gene: 119773399

Following gene: 119773401

Centisome position: 6.89

GC content: 57.52

Gene sequence:

>1224_bases
ATGGCAATCGAAAACAGCAAAACCGGCGTCAAGAAAGTAGTACTGGCCTATTCAGGCGGTCTGGACACCTCGGCCATCAT
TCCGTGGTTGAAGGAAAACTATGATAACTGCGAAATCGTCGCCTTCTGTGCCGACGTGGGCCAGGGTGAAGAAGAGCTGG
TGGGCCTGACCGAAAAGGCCCTTGCCTCAGGTGCGAGTGAGTGTCACATCGTTGACCTGAAAGAAGAATTTGTTAAAGAC
TACATCTACCCCACCATAGCCACTGGTGCCATTTACGAAGGTACTTATCTGCTGGGTACCTCCATGGCCCGTCCTATCAT
CGCCAAGGCGCAGGTAGAAGTGGCCCGTAAAGTGGGCGCCGATGCCCTGTGCCACGGTTGCACCGGTAAGGGTAATGACC
AGGTGCGTTTCGAAGGCTGCTTCGCTGCGCTTGCGCCCGATTTGAAAGTGATTGCCCCATGGCGTGAGTGGGACATGGAA
AGCCGTGAAGATCTGCTGGCGTATCTCGCCGAGCGCAACATCAAGACCTCGGCCTCTGCCACCAAGATTTACAGCCGCGA
TGCCAACGCCTGGCACATCTCCCACGAAGGTGGCGAGCTGGAAGACCCTTGGAACGAGCCATCCAAGCAGGTATGGACCA
TGACTGTGGACCCGCTGGATGCGCCTGACGAGCCTGAGTACGTGACCCTCAAGATTGAAAATGCCCGGGTGACCGAGGTG
AATGGCGAGGCCCTGAGCCCATATCAGGCGCTGATGAAGCTTAACGCCATTGCCGCGCCCCATGGCGTTGGCCGTATCGA
CATTACCGAAAACCGCCTGGTGGGCATGAAGTCCCGCGGCTGCTACGAAACCCCCGGCGGTACCGTGATGTTTGCTGCCC
TGCGTGCGGTGGAAGAGCTGGTGCTGGACAAGAGCAGCCGTGAATGGCGTGAGCAGGTTGCCGCCCGTATGGCGCACCTG
GTTTATGACGGCCGTTGGTTCACGCCTCTGTGTGACTCTCTGCTGGCCGCCTCCGAGTCGCTGGCGACCATGGTCAACGG
TGAGGTAGTGGTTAAGCTCTATAAGGGGCAGGCCGTTCCTGTGAAGAAGCGTTCACCCAACAGCCTCTACTCAGAAGCCT
TTGCCACCTTTGGTCAGGACAGCGTGTACGATCAGAAACACGCCGAAGGCTTTATCCGCCTTTACTCGCTGGCAAGCCGC
ATCCGCGCTTACAACACCAAGTAA

Upstream 100 bases:

>100_bases
AGAACCGCATGCACGCACAAAACGCAGTGTTGGTCACACTGCTGGGATAAATTCAATCTATTGAGTCAACGGCGCCCCAC
GCGCCTCTTGGGAAACAGTT

Downstream 100 bases:

>100_bases
GCCGCCTTGTCAGTCAGGGGGCCAATTTGGCCCCCATTCATTTTTAGTTCCGGGCACGCCCGGGCAGAAGGAGAATTGCT
ATGGCCTTGTGGGGCGGCAG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MAIENSKTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIVAFCADVGQGEEELVGLTEKALASGASECHIVDLKEEFVKD
YIYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWDME
SREDLLAYLAERNIKTSASATKIYSRDANAWHISHEGGELEDPWNEPSKQVWTMTVDPLDAPDEPEYVTLKIENARVTEV
NGEALSPYQALMKLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAVEELVLDKSSREWREQVAARMAHL
VYDGRWFTPLCDSLLAASESLATMVNGEVVVKLYKGQAVPVKKRSPNSLYSEAFATFGQDSVYDQKHAEGFIRLYSLASR
IRAYNTK

Sequences:

>Translated_407_residues
MAIENSKTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIVAFCADVGQGEEELVGLTEKALASGASECHIVDLKEEFVKD
YIYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWDME
SREDLLAYLAERNIKTSASATKIYSRDANAWHISHEGGELEDPWNEPSKQVWTMTVDPLDAPDEPEYVTLKIENARVTEV
NGEALSPYQALMKLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAVEELVLDKSSREWREQVAARMAHL
VYDGRWFTPLCDSLLAASESLATMVNGEVVVKLYKGQAVPVKKRSPNSLYSEAFATFGQDSVYDQKHAEGFIRLYSLASR
IRAYNTK
>Mature_406_residues
AIENSKTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIVAFCADVGQGEEELVGLTEKALASGASECHIVDLKEEFVKDY
IYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWDMES
REDLLAYLAERNIKTSASATKIYSRDANAWHISHEGGELEDPWNEPSKQVWTMTVDPLDAPDEPEYVTLKIENARVTEVN
GEALSPYQALMKLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAVEELVLDKSSREWREQVAARMAHLV
YDGRWFTPLCDSLLAASESLATMVNGEVVVKLYKGQAVPVKKRSPNSLYSEAFATFGQDSVYDQKHAEGFIRLYSLASRI
RAYNTK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=403, Percent_Identity=41.6873449131514, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI16950633, Length=403, Percent_Identity=41.6873449131514, Blast_Score=296, Evalue=3e-80,
Organism=Escherichia coli, GI1789563, Length=369, Percent_Identity=27.3712737127371, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324514, Length=408, Percent_Identity=41.1764705882353, Blast_Score=302, Evalue=7e-83,
Organism=Drosophila melanogaster, GI21358151, Length=408, Percent_Identity=41.6666666666667, Blast_Score=300, Evalue=8e-82,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_SHEAM (A1S264)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_926140.1
- ProteinModelPortal:   A1S264
- SMR:   A1S264
- STRING:   A1S264
- GeneID:   4602515
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_0259
- NMPDR:   fig|326297.7.peg.249
- eggNOG:   COG0137
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- PhylomeDB:   A1S264
- ProtClustDB:   PRK00509
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44791; Mature: 44660

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 44-44 BINDING 96-96 BINDING 101-101 BINDING 126-126 BINDING 128-128 BINDING 132-132 BINDING 132-132 BINDING 133-133 BINDING 136-136 BINDING 185-185 BINDING 194-194 BINDING 270-270 BINDING 282-282

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIENSKTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIVAFCADVGQGEEELVGLTEKA
CCCCCCCCCHHEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHH
LASGASECHIVDLKEEFVKDYIYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVARKVGA
HHCCCCCEEEECHHHHHHHHHHCHHHCCCCEECCEEEECCHHCCHHHHHHHHHHHHHHCH
DALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWDMESREDLLAYLAERNIKTSASA
HHHHCCCCCCCCCCEEEEEEHHHHCCCCEEEECHHHCCCCCHHHHHHHHHHCCCCCCCCH
TKIYSRDANAWHISHEGGELEDPWNEPSKQVWTMTVDPLDAPDEPEYVTLKIENARVTEV
HHHEECCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCEEEEC
NGEALSPYQALMKLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAVEEL
CCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHHH
VLDKSSREWREQVAARMAHLVYDGRWFTPLCDSLLAASESLATMVNGEVVVKLYKGQAVP
HHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCC
VKKRSPNSLYSEAFATFGQDSVYDQKHAEGFIRLYSLASRIRAYNTK
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AIENSKTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIVAFCADVGQGEEELVGLTEKA
CCCCCCCCHHEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHH
LASGASECHIVDLKEEFVKDYIYPTIATGAIYEGTYLLGTSMARPIIAKAQVEVARKVGA
HHCCCCCEEEECHHHHHHHHHHCHHHCCCCEECCEEEECCHHCCHHHHHHHHHHHHHHCH
DALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWDMESREDLLAYLAERNIKTSASA
HHHHCCCCCCCCCCEEEEEEHHHHCCCCEEEECHHHCCCCCHHHHHHHHHHCCCCCCCCH
TKIYSRDANAWHISHEGGELEDPWNEPSKQVWTMTVDPLDAPDEPEYVTLKIENARVTEV
HHHEECCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECCCEEEEC
NGEALSPYQALMKLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAVEEL
CCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHHH
VLDKSSREWREQVAARMAHLVYDGRWFTPLCDSLLAASESLATMVNGEVVVKLYKGQAVP
HHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCC
VKKRSPNSLYSEAFATFGQDSVYDQKHAEGFIRLYSLASRIRAYNTK
CCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA