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The map label for this gene is dsbE [H]

Identifier: 119773390

GI number: 119773390

Start: 273781

End: 274368

Strand: Direct

Name: dsbE [H]

Synonym: Sama_0249

Alternate gene names: 119773390

Gene position: 273781-274368 (Clockwise)

Preceding gene: 119773389

Following gene: 119773391

Centisome position: 6.36

GC content: 52.04

Gene sequence:

>588_bases
ATGAAGAAGCTGGTCTTATTCATTCCATTGGTACTGTTTCTGGGGATGGGGATATTCCTCTACAAGGGCTTGTTCCTGAA
TCCTCAAAAACTGGAGTCGGCGCTTGAAGGTAAGCCTATTCCGGCGTTTCAGTTAGAGAAACTGGAAACGCCCGGTGAGA
TGATCACTCAGGAAGACCTGAAAGGTCAGGTGTCCCTGTTGAACGTCTGGGCAACCTGGTGTCCATCCTGTAAGTATGAG
CATCCGTTTTTGATGACGTTGACCCGTCAGAAGTTGGTGCCTATTTACGGTATCAATTATCGCGATGAGCGTGCAGAAGC
TATTCGCGAGCTTCGCCATCAGGGTGACCCCTATGTACTGAACATCTTCGATAAAGATGGTCGTCTAGGGTTGGACCTGG
GGGTGTATGGTGCGCCGGAAACGTTTCTGGTTGACCATAAGGGTATTATTCGCTTCCGCTTTGCCGGTCCGGTTGATCAG
CGTGTGTGGAGTGAGACTCTTTACCCCATGGTTCAGCAGTTGCAGCAGGAGGCCGCTGCGGAAGCGGCCGCTTCCGGTAA
AGCCACTGCGACAGGAGCCGCCTCATGA

Upstream 100 bases:

>100_bases
CGGCCTCGGATAAACGTTACCGCGTAAAGGCAGCGGCTTCCGACAAAGCTAAAGAAGCGACTAAAGGTAACCTGGCTACA
GCCTGAAAATTGGAGAAACG

Downstream 100 bases:

>100_bases
AACCATTAAACCTGTTGTTGGCAATGGTGCTGTTCTGTATCAGCACCCTGACATTCGCAACGCCGGTGGACACCTATGAA
TTTAAGTCTGCCGACAATCA

Product: thiol:disulfide interchange protein DsbE

Products: NA

Alternate protein names: Cytochrome c biogenesis protein CcmG [H]

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MKKLVLFIPLVLFLGMGIFLYKGLFLNPQKLESALEGKPIPAFQLEKLETPGEMITQEDLKGQVSLLNVWATWCPSCKYE
HPFLMTLTRQKLVPIYGINYRDERAEAIRELRHQGDPYVLNIFDKDGRLGLDLGVYGAPETFLVDHKGIIRFRFAGPVDQ
RVWSETLYPMVQQLQQEAAAEAAASGKATATGAAS

Sequences:

>Translated_195_residues
MKKLVLFIPLVLFLGMGIFLYKGLFLNPQKLESALEGKPIPAFQLEKLETPGEMITQEDLKGQVSLLNVWATWCPSCKYE
HPFLMTLTRQKLVPIYGINYRDERAEAIRELRHQGDPYVLNIFDKDGRLGLDLGVYGAPETFLVDHKGIIRFRFAGPVDQ
RVWSETLYPMVQQLQQEAAAEAAASGKATATGAAS
>Mature_195_residues
MKKLVLFIPLVLFLGMGIFLYKGLFLNPQKLESALEGKPIPAFQLEKLETPGEMITQEDLKGQVSLLNVWATWCPSCKYE
HPFLMTLTRQKLVPIYGINYRDERAEAIRELRHQGDPYVLNIFDKDGRLGLDLGVYGAPETFLVDHKGIIRFRFAGPVDQ
RVWSETLYPMVQQLQQEAAAEAAASGKATATGAAS

Specific function: Involved in disulfide bond formation. Catalyzes a late, reductive step in the assembly of periplasmic c-type cytochromes, probably the reduction of disulfide bonds of the apocytochrome c to allow covalent linkage with the heme. Possible subunit of a heme

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1788523, Length=185, Percent_Identity=50.2702702702703, Blast_Score=185, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004799
- InterPro:   IPR013740
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF08534 Redoxin [H]

EC number: NA

Molecular weight: Translated: 21886; Mature: 21886

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLVLFIPLVLFLGMGIFLYKGLFLNPQKLESALEGKPIPAFQLEKLETPGEMITQEDL
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHH
KGQVSLLNVWATWCPSCKYEHPFLMTLTRQKLVPIYGINYRDERAEAIRELRHQGDPYVL
CCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHCCEEECCCCCHHHHHHHHHHHHCCCCEEE
NIFDKDGRLGLDLGVYGAPETFLVDHKGIIRFRFAGPVDQRVWSETLYPMVQQLQQEAAA
EEECCCCCEEEEEECCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
EAAASGKATATGAAS
HHHHCCCCCCCCCCC
>Mature Secondary Structure
MKKLVLFIPLVLFLGMGIFLYKGLFLNPQKLESALEGKPIPAFQLEKLETPGEMITQEDL
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHH
KGQVSLLNVWATWCPSCKYEHPFLMTLTRQKLVPIYGINYRDERAEAIRELRHQGDPYVL
CCHHHHHHHHHHHCCCCCCCCCEEEEEHHHHCCEEECCCCCHHHHHHHHHHHHCCCCEEE
NIFDKDGRLGLDLGVYGAPETFLVDHKGIIRFRFAGPVDQRVWSETLYPMVQQLQQEAAA
EEECCCCCEEEEEECCCCCCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
EAAASGKATATGAAS
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]