Click here to switch to the map view.

The map label for this gene is pnp

Identifier: 119717401

GI number: 119717401

Start: 3375064

End: 3377292

Strand: Reverse

Name: pnp

Synonym: Noca_3177

Alternate gene names: 119717401

Gene position: 3377292-3375064 (Counterclockwise)

Preceding gene: 119717402

Following gene: 119717400

Centisome position: 67.74

GC content: 69.27

Gene sequence:

>2229_bases
TTGACCGACCCCGAGATCTCTGCCGTCGAGACCGTTCTGGACAACGGCAAGTTCGGCACCCGCACCGTCAAGTTCGAGAC
CGGCCTACTGGCCCGGCAGGCTGCCGGCGCCGTGACCGCCTACCTCGACGAGGACACCATGCTCCTGTCGGCCACCACGG
CCGGCAAGCACCCGAAGGACCACTTCGACTTCTTCCCGCTCACCATCGACGTCGAGGAGCGGATGTACGCCGCGGGCAAG
ATCCCCGGCTCGTTCTTCCGCAGCGAGGGCCGTCCGGGCGAGGACGCGATCCTCACCTGCCGCCTCATCGACCGGCCGCT
GCGCCCGACCTTCAAGAAGGGCCTGCGCAACGAGGTCCAGGTCGTCATCACCGTGATGGCGCTCAACCCCGACACGCCGT
ACGACGTGCTCGCGATCAACGCCGCCTCGCTCTCCACCCAGCTCTCGGGCCTGCCGTTCTCCGGCCCCGTCGGCGGCGTC
CGCGTGGCGCTGATCGAGGGCCAGTGGGTCGCCTTCCCGACCCACAGCCAGCTCGAGAACGCCGTGTTCGACATGGTGGT
CGCCGGACGCGTCACCGAGACCGGTGACGTCGCGATCATGATGGTCGAGGCCGAGGCCACCGAGCAGACCTGGGACCTGG
TCCGCTCCGGCACCCAGGCGCCGACCGAGGAGATCGTGGCCGGCGGTCTCGACGCCGCCAAGCCGTTCATCAAGCAGCTC
TGCGAGGCGCAGGTCGAGCTGGCGAACGTCGCGGCGAAGCCGGTCCAGGACTTCCCCGTCTTCCTCGACTACGAGGACGA
CGTGTACGACGCGGTCGAAGCGGCCGTGAAGGCCGAGCTGGCCGCGGCGATGAAGATCGCCGACAAGCAGGAGCGCAACG
ACCGCACCGACGAGCTCAAGGACGAGGTCCTGGCCAAGCTCGGCGAGCAGTTCGAGGGCCGTGAGAAGGAGATCGGCGCG
GCGTTCCGCTCGGTCAACAAGCAGGTCGTCCGGGAGAGCATCCTGCGCGACAAGGTCCGCATCGACGGCCGCGGCCTGGC
CGACATCCGGCCGCTGCACGCCGAGGTCGGCGTGATTCCGCGGGTGCACGGCTCGGCGCTGTTCGAGCGTGGGGAGACCC
AGATCCTGGGCGTCACGACGCTGGACATGCTCAAGATGGAGCAGCAGCTCGACACGCTCTCGCCGGAGAAGCACCGCCGT
TACATGCACAAGTACGTCTTCCCGCCGTTCTCCACCGGCGAGACCGGCCGGGTCGGCTCGCCGAAGCGCCGCGAGGTCGG
CCACGGCGCGCTGGCGCGCCGGGCGCTGCTGCCGGTGCTGCCCACGCGCGAGGAGTTCCCCTACGCGATCCGCCAGCTCT
CCGAGGCGATGGGCTCCAACGGCTCGACCTCGATGGGCTCGGTCTGCGCGTCCACGCTCGCACTGCTCCAGGCCGGTGTG
CCGCTGAAGGCCCCGGTCGCCGGCATCGCGATGGGCCTGGTCTCCGGCGAGATCGACGGCCAGCTCCAGTACGTCGCCCT
CACCGACATCCTCGGTGCCGAGGACGCGTTCGGCGACATGGACTTCAAGGTCGCCGGCACCCGCGAGTTCGTCACCGCGC
TGCAGCTGGACACCAAGCTCGACGGCATCCCGGCCGAGGTCCTCGCCCAGGCGCTGACCCAGGCGCGCGACGCCCGCCTG
ACCATCCTCGACGTGATGGCCGAGGCCATCGACGAGCCCGAGGAGATGTCGGCGTACGCCCCGCGGATCATCACGATCAC
GATCCCGGTCGACAAGATCGGCGAGGTGATCGGCCCCAAGGGCAAGATCATCAACCAGATCCAGGACGACACCGGCGCGT
CGATCTCGATCGAGGACGACGGCACGATCTACATCGGTGCGACCAACGGCGAGGCGGCCGAGGCGGCCAAGAACGCCGTC
AACGCGATCGCCAACCCGACCATGCCCGAGGTCGGCGAGCGCTACCTCGGCACGGTCGTCAAGACGACCAACTTCGGCGC
GTTCGTCTCGCTGATGCCCGGCAAGGACGGCCTGCTGCACATCAGCAAGCTCCGCTCGCTGGCCGGCGGCAAGCGGGTCG
ACGCGGTCGAGGACGTCGTCTCGGTCGGCCAGAAGATCCAGGTCCAGATCGCCGAGATCGACGACCGCGGGAAGCTGTCG
CTGGTCCCGGTCGTCGAGGGCGACCAGGCCGACTCCGACACCGCCGAGGCCGGCTCCGAGGAGGAGTGA

Upstream 100 bases:

>100_bases
CCGGCCGGCTCTGCCGCGCAGATCTGCGGGCCTCGATCGAAGACCGGCTCGCAGCAGCGGGACGCCGCGGATCGCTCCGC
GACAGAAGAGGGACACACCC

Downstream 100 bases:

>100_bases
CGCCCCGGTCGGCCGAAGCCTCACCGGTGACCCGCCAGAGCAGCTCTGGCGGGTCACCGTCTTCCCGGGGCACGACGCGC
ACGCTGCAGACGACCACCGA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 742; Mature: 741

Protein sequence:

>742_residues
MTDPEISAVETVLDNGKFGTRTVKFETGLLARQAAGAVTAYLDEDTMLLSATTAGKHPKDHFDFFPLTIDVEERMYAAGK
IPGSFFRSEGRPGEDAILTCRLIDRPLRPTFKKGLRNEVQVVITVMALNPDTPYDVLAINAASLSTQLSGLPFSGPVGGV
RVALIEGQWVAFPTHSQLENAVFDMVVAGRVTETGDVAIMMVEAEATEQTWDLVRSGTQAPTEEIVAGGLDAAKPFIKQL
CEAQVELANVAAKPVQDFPVFLDYEDDVYDAVEAAVKAELAAAMKIADKQERNDRTDELKDEVLAKLGEQFEGREKEIGA
AFRSVNKQVVRESILRDKVRIDGRGLADIRPLHAEVGVIPRVHGSALFERGETQILGVTTLDMLKMEQQLDTLSPEKHRR
YMHKYVFPPFSTGETGRVGSPKRREVGHGALARRALLPVLPTREEFPYAIRQLSEAMGSNGSTSMGSVCASTLALLQAGV
PLKAPVAGIAMGLVSGEIDGQLQYVALTDILGAEDAFGDMDFKVAGTREFVTALQLDTKLDGIPAEVLAQALTQARDARL
TILDVMAEAIDEPEEMSAYAPRIITITIPVDKIGEVIGPKGKIINQIQDDTGASISIEDDGTIYIGATNGEAAEAAKNAV
NAIANPTMPEVGERYLGTVVKTTNFGAFVSLMPGKDGLLHISKLRSLAGGKRVDAVEDVVSVGQKIQVQIAEIDDRGKLS
LVPVVEGDQADSDTAEAGSEEE

Sequences:

>Translated_742_residues
MTDPEISAVETVLDNGKFGTRTVKFETGLLARQAAGAVTAYLDEDTMLLSATTAGKHPKDHFDFFPLTIDVEERMYAAGK
IPGSFFRSEGRPGEDAILTCRLIDRPLRPTFKKGLRNEVQVVITVMALNPDTPYDVLAINAASLSTQLSGLPFSGPVGGV
RVALIEGQWVAFPTHSQLENAVFDMVVAGRVTETGDVAIMMVEAEATEQTWDLVRSGTQAPTEEIVAGGLDAAKPFIKQL
CEAQVELANVAAKPVQDFPVFLDYEDDVYDAVEAAVKAELAAAMKIADKQERNDRTDELKDEVLAKLGEQFEGREKEIGA
AFRSVNKQVVRESILRDKVRIDGRGLADIRPLHAEVGVIPRVHGSALFERGETQILGVTTLDMLKMEQQLDTLSPEKHRR
YMHKYVFPPFSTGETGRVGSPKRREVGHGALARRALLPVLPTREEFPYAIRQLSEAMGSNGSTSMGSVCASTLALLQAGV
PLKAPVAGIAMGLVSGEIDGQLQYVALTDILGAEDAFGDMDFKVAGTREFVTALQLDTKLDGIPAEVLAQALTQARDARL
TILDVMAEAIDEPEEMSAYAPRIITITIPVDKIGEVIGPKGKIINQIQDDTGASISIEDDGTIYIGATNGEAAEAAKNAV
NAIANPTMPEVGERYLGTVVKTTNFGAFVSLMPGKDGLLHISKLRSLAGGKRVDAVEDVVSVGQKIQVQIAEIDDRGKLS
LVPVVEGDQADSDTAEAGSEEE
>Mature_741_residues
TDPEISAVETVLDNGKFGTRTVKFETGLLARQAAGAVTAYLDEDTMLLSATTAGKHPKDHFDFFPLTIDVEERMYAAGKI
PGSFFRSEGRPGEDAILTCRLIDRPLRPTFKKGLRNEVQVVITVMALNPDTPYDVLAINAASLSTQLSGLPFSGPVGGVR
VALIEGQWVAFPTHSQLENAVFDMVVAGRVTETGDVAIMMVEAEATEQTWDLVRSGTQAPTEEIVAGGLDAAKPFIKQLC
EAQVELANVAAKPVQDFPVFLDYEDDVYDAVEAAVKAELAAAMKIADKQERNDRTDELKDEVLAKLGEQFEGREKEIGAA
FRSVNKQVVRESILRDKVRIDGRGLADIRPLHAEVGVIPRVHGSALFERGETQILGVTTLDMLKMEQQLDTLSPEKHRRY
MHKYVFPPFSTGETGRVGSPKRREVGHGALARRALLPVLPTREEFPYAIRQLSEAMGSNGSTSMGSVCASTLALLQAGVP
LKAPVAGIAMGLVSGEIDGQLQYVALTDILGAEDAFGDMDFKVAGTREFVTALQLDTKLDGIPAEVLAQALTQARDARLT
ILDVMAEAIDEPEEMSAYAPRIITITIPVDKIGEVIGPKGKIINQIQDDTGASISIEDDGTIYIGATNGEAAEAAKNAVN
AIANPTMPEVGERYLGTVVKTTNFGAFVSLMPGKDGLLHISKLRSLAGGKRVDAVEDVVSVGQKIQVQIAEIDDRGKLSL
VPVVEGDQADSDTAEAGSEEE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=723, Percent_Identity=34.0248962655602, Blast_Score=373, Evalue=1e-103,
Organism=Escherichia coli, GI145693187, Length=712, Percent_Identity=46.0674157303371, Blast_Score=566, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI115534063, Length=679, Percent_Identity=31.5169366715758, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI281362905, Length=728, Percent_Identity=35.1648351648352, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24651641, Length=728, Percent_Identity=35.1648351648352, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24651643, Length=728, Percent_Identity=35.1648351648352, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI161079377, Length=654, Percent_Identity=35.9327217125382, Blast_Score=363, Evalue=1e-100,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_NOCSJ (A1SLJ4)

Other databases:

- EMBL:   CP000509
- RefSeq:   YP_924366.1
- ProteinModelPortal:   A1SLJ4
- SMR:   A1SLJ4
- STRING:   A1SLJ4
- GeneID:   4599040
- GenomeReviews:   CP000509_GR
- KEGG:   nca:Noca_3177
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   NSP35761:NOCA_3177-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR014069
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591
- TIGRFAMs:   TIGR02696

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 79753; Mature: 79621

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPEISAVETVLDNGKFGTRTVKFETGLLARQAAGAVTAYLDEDTMLLSATTAGKHPKD
CCCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCEEEEECCCEEEEEECCCCCCCCC
HFDFFPLTIDVEERMYAAGKIPGSFFRSEGRPGEDAILTCRLIDRPLRPTFKKGLRNEVQ
CCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEHHHCCCCCHHHHHHCCCCEE
VVITVMALNPDTPYDVLAINAASLSTQLSGLPFSGPVGGVRVALIEGQWVAFPTHSQLEN
EEEEEEEECCCCCCEEEEEEHHHHHHHHCCCCCCCCCCCEEEEEEECCEEECCCHHHHHH
AVFDMVVAGRVTETGDVAIMMVEAEATEQTWDLVRSGTQAPTEEIVAGGLDAAKPFIKQL
HHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHH
CEAQVELANVAAKPVQDFPVFLDYEDDVYDAVEAAVKAELAAAMKIADKQERNDRTDELK
HHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DEVLAKLGEQFEGREKEIGAAFRSVNKQVVRESILRDKVRIDGRGLADIRPLHAEVGVIP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHCCCC
RVHGSALFERGETQILGVTTLDMLKMEQQLDTLSPEKHRRYMHKYVFPPFSTGETGRVGS
CCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
PKRREVGHGALARRALLPVLPTREEFPYAIRQLSEAMGSNGSTSMGSVCASTLALLQAGV
CHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
PLKAPVAGIAMGLVSGEIDGQLQYVALTDILGAEDAFGDMDFKVAGTREFVTALQLDTKL
CCCCCHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCCCCCCCEEEECHHHHHHHHHHCCCC
DGIPAEVLAQALTQARDARLTILDVMAEAIDEPEEMSAYAPRIITITIPVDKIGEVIGPK
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHCCEEEEEEECHHHHHHHHCCC
GKIINQIQDDTGASISIEDDGTIYIGATNGEAAEAAKNAVNAIANPTMPEVGERYLGTVV
HHHHHHHCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
KTTNFGAFVSLMPGKDGLLHISKLRSLAGGKRVDAVEDVVSVGQKIQVQIAEIDDRGKLS
EECCCCEEEEECCCCCCCEEHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCEE
LVPVVEGDQADSDTAEAGSEEE
EEEEECCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
TDPEISAVETVLDNGKFGTRTVKFETGLLARQAAGAVTAYLDEDTMLLSATTAGKHPKD
CCCHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCEEEEECCCEEEEEECCCCCCCCC
HFDFFPLTIDVEERMYAAGKIPGSFFRSEGRPGEDAILTCRLIDRPLRPTFKKGLRNEVQ
CCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEHHHCCCCCHHHHHHCCCCEE
VVITVMALNPDTPYDVLAINAASLSTQLSGLPFSGPVGGVRVALIEGQWVAFPTHSQLEN
EEEEEEEECCCCCCEEEEEEHHHHHHHHCCCCCCCCCCCEEEEEEECCEEECCCHHHHHH
AVFDMVVAGRVTETGDVAIMMVEAEATEQTWDLVRSGTQAPTEEIVAGGLDAAKPFIKQL
HHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHH
CEAQVELANVAAKPVQDFPVFLDYEDDVYDAVEAAVKAELAAAMKIADKQERNDRTDELK
HHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
DEVLAKLGEQFEGREKEIGAAFRSVNKQVVRESILRDKVRIDGRGLADIRPLHAEVGVIP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHCCCC
RVHGSALFERGETQILGVTTLDMLKMEQQLDTLSPEKHRRYMHKYVFPPFSTGETGRVGS
CCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
PKRREVGHGALARRALLPVLPTREEFPYAIRQLSEAMGSNGSTSMGSVCASTLALLQAGV
CHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
PLKAPVAGIAMGLVSGEIDGQLQYVALTDILGAEDAFGDMDFKVAGTREFVTALQLDTKL
CCCCCHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCCCCCCCEEEECHHHHHHHHHHCCCC
DGIPAEVLAQALTQARDARLTILDVMAEAIDEPEEMSAYAPRIITITIPVDKIGEVIGPK
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHCCEEEEEEECHHHHHHHHCCC
GKIINQIQDDTGASISIEDDGTIYIGATNGEAAEAAKNAVNAIANPTMPEVGERYLGTVV
HHHHHHHCCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
KTTNFGAFVSLMPGKDGLLHISKLRSLAGGKRVDAVEDVVSVGQKIQVQIAEIDDRGKLS
EECCCCEEEEECCCCCCCEEHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCEE
LVPVVEGDQADSDTAEAGSEEE
EEEEECCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA