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The map label for this gene is murE [H]

Identifier: 119717292

GI number: 119717292

Start: 3266753

End: 3268267

Strand: Reverse

Name: murE [H]

Synonym: Noca_3068

Alternate gene names: 119717292

Gene position: 3268267-3266753 (Counterclockwise)

Preceding gene: 119717293

Following gene: 119717291

Centisome position: 65.55

GC content: 75.51

Gene sequence:

>1515_bases
GTGGTGAGCGACTCCAACCGGCCCGCGCAGCCGCCCCGGACGCCGCTCGCGACCGTGGCGGCCTGGGCCGGTGCCGAGCT
GCGCGGCAACCCCGCGGGCCTGGAGGTCAGCGGCATCTCGTTGTCCTCGCAACGGATCCGGCCCGGTGACCTCTACGCGG
CGCTTCCCGGCAGCCGCGCCCACGGCATCGAGTTCGCCCAGGACGCGCTCGCGGCCGGCGCGGTCGCGGTCTTCACCGAC
GAGGCGGGAGCCGACCGGGGCCCCGCGGGGGCTCCGCACCTGGTCGTCGACCGGCCCCGCGCCGTGCTCGGCCGGCTCTC
GGCGCGCATCTACGGCGAGCCCGCGGCGTCGATGCGGATGGTCGGGGTCACCGGCACCCAGGGCAAGACCACCACGACCC
GGCTGCTCGAGGCCGGCCTCCAGCAGGCCGGGGTGCCGGCCGCCGTGGTCGGCACGGTCGGCACCCGGATCGCCGGCGAG
GAGGTGCGGTCGTCGCTGACCACGCCCGAGGCGCCCGACCTCCATGCCCTGTTCGCGGTGATGCGCGAGCGCGACGTGCG
CGCCTGCGCGATGGAGGTCTCCAGCCACGCACTGGTGATGGGCCGGGTGGACGGGGTCGTGTTCGACGTCGCGGTGTTCT
TGAACCTGGGCCGCGACCACCTCGACTTCCACCGCGACCTCGAGGACTACTACGCGGCCAAGGCCTCGCTGTTCACCCCC
GAGCGCGCCCGGTTCGCCCTCGTGAACGTCGACGACGCGCACGGCCGCCGGCTGGCCGAGGAGGCCACCGTGCCGGTCCG
GACGTTCTCGATGGGCCGGGGAGACGACGCACAGCGGGCCGACTGGCGGGCTGTCGACGTGCGGCTGCGGGCCACCGGCT
CGGAGTTCGTCGTCGTCGCCCCCGACGGCCGCCGGATCCCGGCCGGGTGCGCGCTGCCCGGCGACTTCAACGTCGCCAAC
ACGCTCGCGGCCGTGGCGGCCGCGGCCGAGGCCGGCCTGGACCCCGCGGTGGTCGCCGCGGGGATCGCCGCGGCCGGGGG
CGTGCCCGGGCGTCTCGAGCGCGTCGACGCCGGTCAGGACTTCCTGGTCGTCGTCGACTACGCCCACAAGCCCGATGCGG
TCGAGGCGGCCATCCGGACGCTGCGCCCGCTCACCGAGGGGCGGGTGATCGTCGTCCTCGGCGCCGGCGGCGACCGCGAC
CCGGGAAAGCGCCCGATCATGGCTGGGATCGCCGCTCGGCTGGCCGATCTGTTGGTGGTGACCGACGACAACCCGCGCAC
GGAGGACCCGGCCGCGATCCGGGCGGCCATGCTCGCCGGGGTCGTGACCGGTCAGGCGATCGAGGTCGGCGGCCGACGGG
CCGCGATCCGCGAGGCGCTGCGCCGGGCCGGACCGGGAGACATCGTGCTGATCGCCGGCAAGGGACACGAGACGGGGCAG
GAGGTCGACGGAGTGGTCCATCCCTTCGACGACCGCGAGGTCGTGCGGGAGGAGATCGTCGCGTGCTCGAGATGA

Upstream 100 bases:

>100_bases
ATCATGAGCTACGCCCTCCAGCGGTACGCCGTCCCGCCGACCGGCACCAAGCCCTCCCGGCTCCCGGTCGAGTGGTAGCC
GGGCGCAGCGATAGCCTCGC

Downstream 100 bases:

>100_bases
CACTGGGGAAGATCGCGAAGGTGGTCGGCGGCACGGTCGCCGGCGACCCCGACATCGTCGTCGACGCGCCGGCGTACGTC
GACTCCCGCCAGGCCGTGCC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MVSDSNRPAQPPRTPLATVAAWAGAELRGNPAGLEVSGISLSSQRIRPGDLYAALPGSRAHGIEFAQDALAAGAVAVFTD
EAGADRGPAGAPHLVVDRPRAVLGRLSARIYGEPAASMRMVGVTGTQGKTTTTRLLEAGLQQAGVPAAVVGTVGTRIAGE
EVRSSLTTPEAPDLHALFAVMRERDVRACAMEVSSHALVMGRVDGVVFDVAVFLNLGRDHLDFHRDLEDYYAAKASLFTP
ERARFALVNVDDAHGRRLAEEATVPVRTFSMGRGDDAQRADWRAVDVRLRATGSEFVVVAPDGRRIPAGCALPGDFNVAN
TLAAVAAAAEAGLDPAVVAAGIAAAGGVPGRLERVDAGQDFLVVVDYAHKPDAVEAAIRTLRPLTEGRVIVVLGAGGDRD
PGKRPIMAGIAARLADLLVVTDDNPRTEDPAAIRAAMLAGVVTGQAIEVGGRRAAIREALRRAGPGDIVLIAGKGHETGQ
EVDGVVHPFDDREVVREEIVACSR

Sequences:

>Translated_504_residues
MVSDSNRPAQPPRTPLATVAAWAGAELRGNPAGLEVSGISLSSQRIRPGDLYAALPGSRAHGIEFAQDALAAGAVAVFTD
EAGADRGPAGAPHLVVDRPRAVLGRLSARIYGEPAASMRMVGVTGTQGKTTTTRLLEAGLQQAGVPAAVVGTVGTRIAGE
EVRSSLTTPEAPDLHALFAVMRERDVRACAMEVSSHALVMGRVDGVVFDVAVFLNLGRDHLDFHRDLEDYYAAKASLFTP
ERARFALVNVDDAHGRRLAEEATVPVRTFSMGRGDDAQRADWRAVDVRLRATGSEFVVVAPDGRRIPAGCALPGDFNVAN
TLAAVAAAAEAGLDPAVVAAGIAAAGGVPGRLERVDAGQDFLVVVDYAHKPDAVEAAIRTLRPLTEGRVIVVLGAGGDRD
PGKRPIMAGIAARLADLLVVTDDNPRTEDPAAIRAAMLAGVVTGQAIEVGGRRAAIREALRRAGPGDIVLIAGKGHETGQ
EVDGVVHPFDDREVVREEIVACSR
>Mature_504_residues
MVSDSNRPAQPPRTPLATVAAWAGAELRGNPAGLEVSGISLSSQRIRPGDLYAALPGSRAHGIEFAQDALAAGAVAVFTD
EAGADRGPAGAPHLVVDRPRAVLGRLSARIYGEPAASMRMVGVTGTQGKTTTTRLLEAGLQQAGVPAAVVGTVGTRIAGE
EVRSSLTTPEAPDLHALFAVMRERDVRACAMEVSSHALVMGRVDGVVFDVAVFLNLGRDHLDFHRDLEDYYAAKASLFTP
ERARFALVNVDDAHGRRLAEEATVPVRTFSMGRGDDAQRADWRAVDVRLRATGSEFVVVAPDGRRIPAGCALPGDFNVAN
TLAAVAAAAEAGLDPAVVAAGIAAAGGVPGRLERVDAGQDFLVVVDYAHKPDAVEAAIRTLRPLTEGRVIVVLGAGGDRD
PGKRPIMAGIAARLADLLVVTDDNPRTEDPAAIRAAMLAGVVTGQAIEVGGRRAAIREALRRAGPGDIVLIAGKGHETGQ
EVDGVVHPFDDREVVREEIVACSR

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=466, Percent_Identity=39.4849785407725, Blast_Score=239, Evalue=4e-64,
Organism=Escherichia coli, GI1786274, Length=373, Percent_Identity=26.5415549597855, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 52559; Mature: 52559

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSDSNRPAQPPRTPLATVAAWAGAELRGNPAGLEVSGISLSSQRIRPGDLYAALPGSRA
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEECEECCCCCCCCCCEEEECCCCCC
HGIEFAQDALAAGAVAVFTDEAGADRGPAGAPHLVVDRPRAVLGRLSARIYGEPAASMRM
CCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCEEE
VGVTGTQGKTTTTRLLEAGLQQAGVPAAVVGTVGTRIAGEEVRSSLTTPEAPDLHALFAV
EEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHH
MRERDVRACAMEVSSHALVMGRVDGVVFDVAVFLNLGRDHLDFHRDLEDYYAAKASLFTP
HHHCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
ERARFALVNVDDAHGRRLAEEATVPVRTFSMGRGDDAQRADWRAVDVRLRATGSEFVVVA
CCCEEEEEECCCHHCCHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEEEECCCEEEEEC
PDGRRIPAGCALPGDFNVANTLAAVAAAAEAGLDPAVVAAGIAAAGGVPGRLERVDAGQD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHCCCCCCC
FLVVVDYAHKPDAVEAAIRTLRPLTEGRVIVVLGAGGDRDPGKRPIMAGIAARLADLLVV
EEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEE
TDDNPRTEDPAAIRAAMLAGVVTGQAIEVGGRRAAIREALRRAGPGDIVLIAGKGHETGQ
ECCCCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCH
EVDGVVHPFDDREVVREEIVACSR
HHCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MVSDSNRPAQPPRTPLATVAAWAGAELRGNPAGLEVSGISLSSQRIRPGDLYAALPGSRA
CCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEEECEECCCCCCCCCCEEEECCCCCC
HGIEFAQDALAAGAVAVFTDEAGADRGPAGAPHLVVDRPRAVLGRLSARIYGEPAASMRM
CCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCCCEEE
VGVTGTQGKTTTTRLLEAGLQQAGVPAAVVGTVGTRIAGEEVRSSLTTPEAPDLHALFAV
EEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHH
MRERDVRACAMEVSSHALVMGRVDGVVFDVAVFLNLGRDHLDFHRDLEDYYAAKASLFTP
HHHCCHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
ERARFALVNVDDAHGRRLAEEATVPVRTFSMGRGDDAQRADWRAVDVRLRATGSEFVVVA
CCCEEEEEECCCHHCCHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEEEECCCEEEEEC
PDGRRIPAGCALPGDFNVANTLAAVAAAAEAGLDPAVVAAGIAAAGGVPGRLERVDAGQD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHCCCCCCC
FLVVVDYAHKPDAVEAAIRTLRPLTEGRVIVVLGAGGDRDPGKRPIMAGIAARLADLLVV
EEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHEEEEE
TDDNPRTEDPAAIRAAMLAGVVTGQAIEVGGRRAAIREALRRAGPGDIVLIAGKGHETGQ
ECCCCCCCCHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCH
EVDGVVHPFDDREVVREEIVACSR
HHCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572948; 12692562 [H]