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The map label for this gene is murF [H]

Identifier: 119717291

GI number: 119717291

Start: 3265380

End: 3266765

Strand: Reverse

Name: murF [H]

Synonym: Noca_3067

Alternate gene names: 119717291

Gene position: 3266765-3265380 (Counterclockwise)

Preceding gene: 119717292

Following gene: 119717290

Centisome position: 65.52

GC content: 73.3

Gene sequence:

>1386_bases
GTGCTCGAGATGACACTGGGGAAGATCGCGAAGGTGGTCGGCGGCACGGTCGCCGGCGACCCCGACATCGTCGTCGACGC
GCCGGCGTACGTCGACTCCCGCCAGGCCGTGCCGGGCGGACTGTTCGTCGCCGTCGTGGGGGAGCACGTCGACGGCCACG
ACTACGCCGCCGGCGCCCATGCGGTGCTCGGCAGCCGGCCGACCGAGGCGCCCACGATCGTCGTCCGCGACCCGATCCCC
GCACTCGGCAAGCTGGCGGCGTACGTCGTCCGCCGGCTGGACGCCACCGTGCTGGCCGTGACCGGCTCCCAGGGCAAGAC
CGGCACCAAGGACTATCTCGCGCGGATCCTGTCCGCCGCCGGGTCGACGGTGGCCACCTCCGGCAGCTTCAACAACGAGC
TCGGCCTGCCGCTGACCGTGCTGCGCGCTCACCCGGAGACGCGGTACCTCGTGCTGGAGATGGGGGCGCGCCGGATCGGC
CACATCAAGCACCTCTGCGAGATCGCGCCCCCGGACGTCGCTGCGGTCCTGAACGTCGGCACCGCCCACATCGGCGAGTT
CGGCAGCCGCGACTCGATCGCGTTCGCGAAGGGCGAGATCGTGGCCGCGCTCCAGCCAGAGGGCACCGCGGTGCTGAACG
CGGGCGACCCCCTGGTCGCCGAGATGACGTCGCGGACCCGGGCCCGGGTGCTCACCTGGGGGCCGGACGACGCCGACGTG
AGCGAGCGCGGCAGCGAGGTCGACGACCTCGGGCGGCCGTCGTTCGAGATCGGGTACGACGACGAGTGGTACCCGGTCCG
GCTCCAGCAGTCCGGTCTGCACCAGGCCCAGAACGCGCTCGCCGCCGCGACCATGGCGCTGGCCGTCGGCATCCCGCTCG
CGGACATCGCGGCCGGCCTGTCGGCCGCGACCGCGTCCTCGCCGATGCGGATGGAGGTGCACGAGCGCGCCGACGGGCTG
ATCGTCGTCAACGACGCCTACAACGCCAACCCCGCCTCCGTCGTCGCCGCGCTCGAGTCCCTGGCCGTCATCGGCCGGCG
CCGGCACCGCCGTACCGTCGCCGTGCTCGGGGAGATGCTCGAGCTCGGCCGCGAGTCCTTCGACGGGCACGTCCGCGTCG
GCCGGGCGGCCGCCCGGCTCGGCACCGACATCGTCGTCGTCGTGGGTGCGGCCGCCGGCGGGATCGTCGAGGGGCTCGAG
GCGGTCGCCGCCTGGCGTGGCGATGGCGTCGTGGCGGAGGAGGTCGAAGAGGCCCTCGAGTGGGTACGCGAGAATGTCCT
GTCCGGTGACGTGGTCCTGGTCAAGGCGTCGCACGGCGTCGCCTTGTGGGAGGTCGCGGACGCACTGCTCGACACCGACC
CGGAGGGAGACCGAACCGAGCCATGA

Upstream 100 bases:

>100_bases
TCGTGCTGATCGCCGGCAAGGGACACGAGACGGGGCAGGAGGTCGACGGAGTGGTCCATCCCTTCGACGACCGCGAGGTC
GTGCGGGAGGAGATCGTCGC

Downstream 100 bases:

>100_bases
GAGCCATCCTGTTCGGAGGCGGTCTGGCCCTGCTCGTCTCGCTGCTCGGCACCCGGGTCGCGATCAACCAGTTCACGCGG
CTGGGCTACGGCCAGGAGAT

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLEMTLGKIAKVVGGTVAGDPDIVVDAPAYVDSRQAVPGGLFVAVVGEHVDGHDYAAGAHAVLGSRPTEAPTIVVRDPIP
ALGKLAAYVVRRLDATVLAVTGSQGKTGTKDYLARILSAAGSTVATSGSFNNELGLPLTVLRAHPETRYLVLEMGARRIG
HIKHLCEIAPPDVAAVLNVGTAHIGEFGSRDSIAFAKGEIVAALQPEGTAVLNAGDPLVAEMTSRTRARVLTWGPDDADV
SERGSEVDDLGRPSFEIGYDDEWYPVRLQQSGLHQAQNALAAATMALAVGIPLADIAAGLSAATASSPMRMEVHERADGL
IVVNDAYNANPASVVAALESLAVIGRRRHRRTVAVLGEMLELGRESFDGHVRVGRAAARLGTDIVVVVGAAAGGIVEGLE
AVAAWRGDGVVAEEVEEALEWVRENVLSGDVVLVKASHGVALWEVADALLDTDPEGDRTEP

Sequences:

>Translated_461_residues
MLEMTLGKIAKVVGGTVAGDPDIVVDAPAYVDSRQAVPGGLFVAVVGEHVDGHDYAAGAHAVLGSRPTEAPTIVVRDPIP
ALGKLAAYVVRRLDATVLAVTGSQGKTGTKDYLARILSAAGSTVATSGSFNNELGLPLTVLRAHPETRYLVLEMGARRIG
HIKHLCEIAPPDVAAVLNVGTAHIGEFGSRDSIAFAKGEIVAALQPEGTAVLNAGDPLVAEMTSRTRARVLTWGPDDADV
SERGSEVDDLGRPSFEIGYDDEWYPVRLQQSGLHQAQNALAAATMALAVGIPLADIAAGLSAATASSPMRMEVHERADGL
IVVNDAYNANPASVVAALESLAVIGRRRHRRTVAVLGEMLELGRESFDGHVRVGRAAARLGTDIVVVVGAAAGGIVEGLE
AVAAWRGDGVVAEEVEEALEWVRENVLSGDVVLVKASHGVALWEVADALLDTDPEGDRTEP
>Mature_461_residues
MLEMTLGKIAKVVGGTVAGDPDIVVDAPAYVDSRQAVPGGLFVAVVGEHVDGHDYAAGAHAVLGSRPTEAPTIVVRDPIP
ALGKLAAYVVRRLDATVLAVTGSQGKTGTKDYLARILSAAGSTVATSGSFNNELGLPLTVLRAHPETRYLVLEMGARRIG
HIKHLCEIAPPDVAAVLNVGTAHIGEFGSRDSIAFAKGEIVAALQPEGTAVLNAGDPLVAEMTSRTRARVLTWGPDDADV
SERGSEVDDLGRPSFEIGYDDEWYPVRLQQSGLHQAQNALAAATMALAVGIPLADIAAGLSAATASSPMRMEVHERADGL
IVVNDAYNANPASVVAALESLAVIGRRRHRRTVAVLGEMLELGRESFDGHVRVGRAAARLGTDIVVVVGAAAGGIVEGLE
AVAAWRGDGVVAEEVEEALEWVRENVLSGDVVLVKASHGVALWEVADALLDTDPEGDRTEP

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=406, Percent_Identity=38.1773399014778, Blast_Score=198, Evalue=7e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48030; Mature: 48030

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEMTLGKIAKVVGGTVAGDPDIVVDAPAYVDSRQAVPGGLFVAVVGEHVDGHDYAAGAH
CCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCH
AVLGSRPTEAPTIVVRDPIPALGKLAAYVVRRLDATVLAVTGSQGKTGTKDYLARILSAA
HHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHC
GSTVATSGSFNNELGLPLTVLRAHPETRYLVLEMGARRIGHIKHLCEIAPPDVAAVLNVG
CCCEEECCCCCCCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHCC
TAHIGEFGSRDSIAFAKGEIVAALQPEGTAVLNAGDPLVAEMTSRTRARVLTWGPDDADV
HHHHCCCCCCCCEEEECCCEEEEECCCCCEEEECCCCHHHHHHCCCCEEEEEECCCCCCH
SERGSEVDDLGRPSFEIGYDDEWYPVRLQQSGLHQAQNALAAATMALAVGIPLADIAAGL
HHCCCCHHHCCCCCEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCC
SAATASSPMRMEVHERADGLIVVNDAYNANPASVVAALESLAVIGRRRHRRTVAVLGEML
HHHHCCCCCEEEHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGRESFDGHVRVGRAAARLGTDIVVVVGAAAGGIVEGLEAVAAWRGDGVVAEEVEEALE
HHHHHCCCCCEEECHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
WVRENVLSGDVVLVKASHGVALWEVADALLDTDPEGDRTEP
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLEMTLGKIAKVVGGTVAGDPDIVVDAPAYVDSRQAVPGGLFVAVVGEHVDGHDYAAGAH
CCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCH
AVLGSRPTEAPTIVVRDPIPALGKLAAYVVRRLDATVLAVTGSQGKTGTKDYLARILSAA
HHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHC
GSTVATSGSFNNELGLPLTVLRAHPETRYLVLEMGARRIGHIKHLCEIAPPDVAAVLNVG
CCCEEECCCCCCCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHCC
TAHIGEFGSRDSIAFAKGEIVAALQPEGTAVLNAGDPLVAEMTSRTRARVLTWGPDDADV
HHHHCCCCCCCCEEEECCCEEEEECCCCCEEEECCCCHHHHHHCCCCEEEEEECCCCCCH
SERGSEVDDLGRPSFEIGYDDEWYPVRLQQSGLHQAQNALAAATMALAVGIPLADIAAGL
HHCCCCHHHCCCCCEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCC
SAATASSPMRMEVHERADGLIVVNDAYNANPASVVAALESLAVIGRRRHRRTVAVLGEML
HHHHCCCCCEEEHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGRESFDGHVRVGRAAARLGTDIVVVVGAAAGGIVEGLEAVAAWRGDGVVAEEVEEALE
HHHHHCCCCCEEECHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHH
WVRENVLSGDVVLVKASHGVALWEVADALLDTDPEGDRTEP
HHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9177243; 12060705 [H]