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The map label for this gene is gltD [H]

Identifier: 119717245

GI number: 119717245

Start: 3218119

End: 3219600

Strand: Reverse

Name: gltD [H]

Synonym: Noca_3021

Alternate gene names: 119717245

Gene position: 3219600-3218119 (Counterclockwise)

Preceding gene: 119717246

Following gene: 119717244

Centisome position: 64.57

GC content: 72.54

Gene sequence:

>1482_bases
ATGGCTGACCCCCGCGGATTCTTGAAGAAGGGCCGGGAGGTCGCCGCGCGGCGCCCGGTCGAGGAGCGGATCCAGGACTG
GAACGAGGTCTACCCGGGCTCCGCCGGCCGGGCCCTGCTCCCGATCATCACCGAGCAGGCCGGGCGTTGCATGGACTGCG
GCATCCCGTTCTGCCACCAGGGCTGCCCGCTGGGGAACATCATCCCCGAGTGGAACGACCTGGTCTGGCGCGACGACTGG
GAGGGCGCGATCGAGCGCCTGCACGCGACCAACAACTTCCCGGACTTCACCGGCCGGCTCTGCCCGGCGCCCTGCGAGAC
GGCCTGCGTGCTCGGCATCAACCAGGATCCGGTGACGATCAAGAACGTCGAGGTCGCGATCATCGACAAGGCGTGGGACT
CCGGGTTCGTGCGCCCGCAGCCGCCGGAGTGGCTCTCGGGTCGCACCGTCGCCGTGGTCGGCTCCGGCCCGGCCGGCCTG
GCCGCCGCTCAGCAGCTGACCCGCGCCGGGCACACGGTCGCGGTCTACGAGCGCGCCGACAAGATCGGCGGGCTGCTGCG
CTACGGCATCCCCGAGTTCAAGATGGAGAAGCAGCACCTCGACAAGCGCTTGGAGCAGATGCGCCGGGAGGGGACCGTCT
TCCGCTCCGGCGTGGAGGTCGGCGCGGAGCTGACCGGCGACCAGCTGCGGGACCGCTACGACGCCGTCGTCCTCGCGATC
GGCTCCACGGTGCCGCGCGAGCTGCCGGCCCCCGGCCGCGAGCTGCGCGGCATCCACCAGGCGATGGACTTCCTCCCGCA
GGCCAACCGGGCCGCGCTCGGCGAGGAGGTCGACGGGCAGATCACCGCGACCGGCAAGCACGTGGTGATCATCGGCGGCG
GCGACACCGGTGCGGACTGCCTCGGCACCTCCACCCGCCAGGGAGCCGCGTCGATCACGCAGCTGGAGATCATGCCCGAG
CCGCCCGAGTCGCGTCCGGCCGGCCAGCCGTGGCCCACGTACCCGATGGTGTTCCGGATCTCCTCGGCCCACGAGGAGGC
CGCGCACCAGAACCTGCAGAGCCGGGTGTACGCCGTCTCCACGAAGGAGTTCCTCGGCGACCAGGACGGCAACGTCCGGG
CGCTCAGGCTGGTCGAGGTGCGCCTCGAGGACGGCCGGTTCGTCGAGGTCGAGGGCTCCGAGCAGGAGATCCCGGCCGAG
CTGGTGCTGCTCGCGATGGGCTTCACCGGTCCGCAGCAGGCCGGCCTGGTCGAGCAGCTCGCCGTGGACCTGGACGAGCG
GGGCAACGTGGCCCGGGACGCGGCGTACATGACGTCGGTCGACGGCGTCTTCGTCGCCGGTGACGCCGGCCGCGGCCAGT
CGCTGATCGTCTGGGCGATCGCCGAGGGCCGGGCCGCGGCCGCCGCGGTGGACACCTACCTGACCGGCTCCACGACGCTG
CCCGCGCCGATCCCGCCCACCGAGCGCCCGCTGGTGGTCTGA

Upstream 100 bases:

>100_bases
AGGCCGAGGCTGACGGCTTGGACGAGAACGAGACCGCCCACGCGATGATGGAGGCGCTCCATGGATGAGCGAAGCGCAGG
AGCAGAGGGGGTGGCGCAGC

Downstream 100 bases:

>100_bases
GGGCCCGCCGCGGTCGGGACCAACGGCACCTTCCGGTCCCGGCCGCGGAGTTACCGGAGGGTTTGGTCTTTGAACGATCA
AAGTCGCGGTAGGGTGAAGC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MADPRGFLKKGREVAARRPVEERIQDWNEVYPGSAGRALLPIITEQAGRCMDCGIPFCHQGCPLGNIIPEWNDLVWRDDW
EGAIERLHATNNFPDFTGRLCPAPCETACVLGINQDPVTIKNVEVAIIDKAWDSGFVRPQPPEWLSGRTVAVVGSGPAGL
AAAQQLTRAGHTVAVYERADKIGGLLRYGIPEFKMEKQHLDKRLEQMRREGTVFRSGVEVGAELTGDQLRDRYDAVVLAI
GSTVPRELPAPGRELRGIHQAMDFLPQANRAALGEEVDGQITATGKHVVIIGGGDTGADCLGTSTRQGAASITQLEIMPE
PPESRPAGQPWPTYPMVFRISSAHEEAAHQNLQSRVYAVSTKEFLGDQDGNVRALRLVEVRLEDGRFVEVEGSEQEIPAE
LVLLAMGFTGPQQAGLVEQLAVDLDERGNVARDAAYMTSVDGVFVAGDAGRGQSLIVWAIAEGRAAAAAVDTYLTGSTTL
PAPIPPTERPLVV

Sequences:

>Translated_493_residues
MADPRGFLKKGREVAARRPVEERIQDWNEVYPGSAGRALLPIITEQAGRCMDCGIPFCHQGCPLGNIIPEWNDLVWRDDW
EGAIERLHATNNFPDFTGRLCPAPCETACVLGINQDPVTIKNVEVAIIDKAWDSGFVRPQPPEWLSGRTVAVVGSGPAGL
AAAQQLTRAGHTVAVYERADKIGGLLRYGIPEFKMEKQHLDKRLEQMRREGTVFRSGVEVGAELTGDQLRDRYDAVVLAI
GSTVPRELPAPGRELRGIHQAMDFLPQANRAALGEEVDGQITATGKHVVIIGGGDTGADCLGTSTRQGAASITQLEIMPE
PPESRPAGQPWPTYPMVFRISSAHEEAAHQNLQSRVYAVSTKEFLGDQDGNVRALRLVEVRLEDGRFVEVEGSEQEIPAE
LVLLAMGFTGPQQAGLVEQLAVDLDERGNVARDAAYMTSVDGVFVAGDAGRGQSLIVWAIAEGRAAAAAVDTYLTGSTTL
PAPIPPTERPLVV
>Mature_492_residues
ADPRGFLKKGREVAARRPVEERIQDWNEVYPGSAGRALLPIITEQAGRCMDCGIPFCHQGCPLGNIIPEWNDLVWRDDWE
GAIERLHATNNFPDFTGRLCPAPCETACVLGINQDPVTIKNVEVAIIDKAWDSGFVRPQPPEWLSGRTVAVVGSGPAGLA
AAQQLTRAGHTVAVYERADKIGGLLRYGIPEFKMEKQHLDKRLEQMRREGTVFRSGVEVGAELTGDQLRDRYDAVVLAIG
STVPRELPAPGRELRGIHQAMDFLPQANRAALGEEVDGQITATGKHVVIIGGGDTGADCLGTSTRQGAASITQLEIMPEP
PESRPAGQPWPTYPMVFRISSAHEEAAHQNLQSRVYAVSTKEFLGDQDGNVRALRLVEVRLEDGRFVEVEGSEQEIPAEL
VLLAMGFTGPQQAGLVEQLAVDLDERGNVARDAAYMTSVDGVFVAGDAGRGQSLIVWAIAEGRAAAAAVDTYLTGSTTLP
APIPPTERPLVV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=467, Percent_Identity=22.6980728051392, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI111118983, Length=152, Percent_Identity=33.5526315789474, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI111118981, Length=152, Percent_Identity=33.5526315789474, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789606, Length=474, Percent_Identity=33.9662447257384, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI1788811, Length=473, Percent_Identity=35.7293868921776, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082180, Length=477, Percent_Identity=33.7526205450734, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788468, Length=459, Percent_Identity=27.4509803921569, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1789243, Length=425, Percent_Identity=27.5294117647059, Blast_Score=118, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17570289, Length=493, Percent_Identity=45.0304259634888, Blast_Score=443, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71984108, Length=456, Percent_Identity=22.5877192982456, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543860, Length=162, Percent_Identity=34.5679012345679, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=496, Percent_Identity=45.7661290322581, Blast_Score=438, Evalue=1e-124,
Organism=Drosophila melanogaster, GI28574881, Length=490, Percent_Identity=52.6530612244898, Blast_Score=502, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24665539, Length=490, Percent_Identity=52.6530612244898, Blast_Score=502, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24665547, Length=490, Percent_Identity=52.6530612244898, Blast_Score=500, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24665543, Length=490, Percent_Identity=52.6530612244898, Blast_Score=500, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24640763, Length=468, Percent_Identity=23.9316239316239, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI18858217, Length=468, Percent_Identity=23.9316239316239, Blast_Score=95, Evalue=9e-20,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53271; Mature: 53140

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADPRGFLKKGREVAARRPVEERIQDWNEVYPGSAGRALLPIITEQAGRCMDCGIPFCHQ
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHCCCEEECCCCHHHC
GCPLGNIIPEWNDLVWRDDWEGAIERLHATNNFPDFTGRLCPAPCETACVLGINQDPVTI
CCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEE
KNVEVAIIDKAWDSGFVRPQPPEWLSGRTVAVVGSGPAGLAAAQQLTRAGHTVAVYERAD
EEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEHHHH
KIGGLLRYGIPEFKMEKQHLDKRLEQMRREGTVFRSGVEVGAELTGDQLRDRYDAVVLAI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCCHHHHHHHCEEEEEE
GSTVPRELPAPGRELRGIHQAMDFLPQANRAALGEEVDGQITATGKHVVIIGGGDTGADC
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEECCCEEEEEECCCCCCHH
LGTSTRQGAASITQLEIMPEPPESRPAGQPWPTYPMVFRISSAHEEAAHQNLQSRVYAVS
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHEEEEE
TKEFLGDQDGNVRALRLVEVRLEDGRFVEVEGSEQEIPAELVLLAMGFTGPQQAGLVEQL
HHHHCCCCCCCEEEEEEEEEEECCCCEEEECCCCCCCCHHEEEEECCCCCCCCCCHHHHH
AVDLDERGNVARDAAYMTSVDGVFVAGDAGRGQSLIVWAIAEGRAAAAAVDTYLTGSTTL
HHHHHCCCCHHHHHHHHEECCCEEEECCCCCCCEEEEEEEECCCCHHHHHHHEECCCCCC
PAPIPPTERPLVV
CCCCCCCCCCCCC
>Mature Secondary Structure 
ADPRGFLKKGREVAARRPVEERIQDWNEVYPGSAGRALLPIITEQAGRCMDCGIPFCHQ
CCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHCCCEEECCCCHHHC
GCPLGNIIPEWNDLVWRDDWEGAIERLHATNNFPDFTGRLCPAPCETACVLGINQDPVTI
CCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCEEE
KNVEVAIIDKAWDSGFVRPQPPEWLSGRTVAVVGSGPAGLAAAQQLTRAGHTVAVYERAD
EEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEHHHH
KIGGLLRYGIPEFKMEKQHLDKRLEQMRREGTVFRSGVEVGAELTGDQLRDRYDAVVLAI
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCEEECCHHHCCCCCCHHHHHHHCEEEEEE
GSTVPRELPAPGRELRGIHQAMDFLPQANRAALGEEVDGQITATGKHVVIIGGGDTGADC
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEECCCEEEEEECCCCCCHH
LGTSTRQGAASITQLEIMPEPPESRPAGQPWPTYPMVFRISSAHEEAAHQNLQSRVYAVS
CCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHEEEEE
TKEFLGDQDGNVRALRLVEVRLEDGRFVEVEGSEQEIPAELVLLAMGFTGPQQAGLVEQL
HHHHCCCCCCCEEEEEEEEEEECCCCEEEECCCCCCCCHHEEEEECCCCCCCCCCHHHHH
AVDLDERGNVARDAAYMTSVDGVFVAGDAGRGQSLIVWAIAEGRAAAAAVDTYLTGSTTL
HHHHHCCCCHHHHHHHHEECCCEEEECCCCCCCEEEEEEEECCCCHHHHHHHEECCCCCC
PAPIPPTERPLVV
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]