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The map label for this gene is pyk [H]

Identifier: 119717244

GI number: 119717244

Start: 3216553

End: 3218016

Strand: Reverse

Name: pyk [H]

Synonym: Noca_3020

Alternate gene names: 119717244

Gene position: 3218016-3216553 (Counterclockwise)

Preceding gene: 119717245

Following gene: 119717242

Centisome position: 64.54

GC content: 70.97

Gene sequence:

>1464_bases
GTGCGTAGAGCGAAGATCGTGTGCACCCTGGGCCCCGCGGTGGCGAACCCTCGACGGATCCGTGAACTGGTCTATGCCGG
CATGGACGTCGCCCGCCTGAACATGAGCCACGGCTCGCACGAGGACCATGCCGAGGCCTACCGCCTCGTGCGCGAGGCCT
CGGACGCGAGCGGCCACGGCGTCGGCATCTTCGCCGACCTGCAGGGCCCGAAGATCCGGCTCGAGCGGTTCGCGAACGGT
CCGGTCGTGCTGACCCGCGGGCAGGCGTGGACGATCACCACCCGCGACGTGCCCGGCGACGACCGCGAGTGCGGGACGAC
GTACAAGGGCCTGCCCGGCGACGTGAGCGTCGGCGACCCGATCCTCATCGACGACGGCAAGGTGCGGCTGCGGGTCACCA
AGGTCGACGGACCGGACGTGCACACCGAGGTGCTCGTGGGCGGCAAGGTCAGCGACAACAAGGGCATCAACCTGCCCGGC
GTCGCCGTGTCCGTGCCGGCGCTGTCGGAGAAGGACATCGAGGACCTGCGCTTCGCGCTGCACCTCACGGTCGACTTCAT
CGCGCTGAGCTTCGTACGCGACGCCAAGGACGTCGAGGACGTCCGGCGGATCATGGACGAGGAGGGCGTGCACCTCCCGG
TCATCGCGAAGATCGAGAAGCCGCAGGCGATCGAGAACCTCGACGAGGTGATCGCGGCGTTCGACGGCTTCATGGTCGCC
CGCGGCGACCTCGGCGTGGAGTGCCCGCTCGAGGACGTCCCGTTCCTGCAGAAGCGGATCGTCGAGAAGGCCCGCCTCAA
CGCCAAGCCCGTCATCGTCGCGACCCAGATGCTGGAGTCGATGATCGGCAACCCCGCGCCGACCCGCGCCGAGGCCTCCG
ACGTCGCGAACGCCGTCCTCGACGGCGCCGACGCGGTGATGCTGTCCGGCGAGACCAGCGTGGGGGAGTACCCCGTCCAC
ACCGTCGAGACGATGGCGCGGATCATCGCGGCGACCGAGGCGCACGCCCTGGCGCAGCCCCCGGGCCGGGCCGCCCTCGC
CGAGATCGACTGGGACCCGCACACCCGCGGCGGCGTGATCGCCAAGGCCGCCGAGGAGGTCGCCGAGCGGGTCGGGGCGA
AGTACGTCGTCGCCTTCACCCAGAGCGGTGACTCCGCGCGCCGGATGTCGCGGCTGCGTGGCCCGATCCCGATCCTCGCG
TTCACGCCGGTCGGGGTGGTGCGCTCGCAGCTCGCGCTGACCTGGGGGATCGAGACGTTCCTGACCCAGCCCGTCGAGCA
CACCGACGAGATGGTCCGTCAGGTCGACGACCAGCTGCTCCGGATCGGCCGGGTCCGCGAGGGTGACCTCGTGGTCATCA
TCGCGGGCGCCCCTCCGGGCATCCCCGGGTCGACGAACGCGCTGCGGATCCACCGGATGGGCGACGCGATCAACCAGGTC
GCGCCGGCGTACCGCCGCAGCTGA

Upstream 100 bases:

>100_bases
GCCCGCCGCGGTCGGGACCAACGGCACCTTCCGGTCCCGGCCGCGGAGTTACCGGAGGGTTTGGTCTTTGAACGATCAAA
GTCGCGGTAGGGTGAAGCAC

Downstream 100 bases:

>100_bases
ACATCAGCGCTTCAGCCCGATCAGCGTGTCGAGCCTGGCCACTGCGGCGCGGGTCGAGACGCTGATCGTCTTCGCGTTCG
ACTGCCGCCACGGGACGTCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MRRAKIVCTLGPAVANPRRIRELVYAGMDVARLNMSHGSHEDHAEAYRLVREASDASGHGVGIFADLQGPKIRLERFANG
PVVLTRGQAWTITTRDVPGDDRECGTTYKGLPGDVSVGDPILIDDGKVRLRVTKVDGPDVHTEVLVGGKVSDNKGINLPG
VAVSVPALSEKDIEDLRFALHLTVDFIALSFVRDAKDVEDVRRIMDEEGVHLPVIAKIEKPQAIENLDEVIAAFDGFMVA
RGDLGVECPLEDVPFLQKRIVEKARLNAKPVIVATQMLESMIGNPAPTRAEASDVANAVLDGADAVMLSGETSVGEYPVH
TVETMARIIAATEAHALAQPPGRAALAEIDWDPHTRGGVIAKAAEEVAERVGAKYVVAFTQSGDSARRMSRLRGPIPILA
FTPVGVVRSQLALTWGIETFLTQPVEHTDEMVRQVDDQLLRIGRVREGDLVVIIAGAPPGIPGSTNALRIHRMGDAINQV
APAYRRS

Sequences:

>Translated_487_residues
MRRAKIVCTLGPAVANPRRIRELVYAGMDVARLNMSHGSHEDHAEAYRLVREASDASGHGVGIFADLQGPKIRLERFANG
PVVLTRGQAWTITTRDVPGDDRECGTTYKGLPGDVSVGDPILIDDGKVRLRVTKVDGPDVHTEVLVGGKVSDNKGINLPG
VAVSVPALSEKDIEDLRFALHLTVDFIALSFVRDAKDVEDVRRIMDEEGVHLPVIAKIEKPQAIENLDEVIAAFDGFMVA
RGDLGVECPLEDVPFLQKRIVEKARLNAKPVIVATQMLESMIGNPAPTRAEASDVANAVLDGADAVMLSGETSVGEYPVH
TVETMARIIAATEAHALAQPPGRAALAEIDWDPHTRGGVIAKAAEEVAERVGAKYVVAFTQSGDSARRMSRLRGPIPILA
FTPVGVVRSQLALTWGIETFLTQPVEHTDEMVRQVDDQLLRIGRVREGDLVVIIAGAPPGIPGSTNALRIHRMGDAINQV
APAYRRS
>Mature_487_residues
MRRAKIVCTLGPAVANPRRIRELVYAGMDVARLNMSHGSHEDHAEAYRLVREASDASGHGVGIFADLQGPKIRLERFANG
PVVLTRGQAWTITTRDVPGDDRECGTTYKGLPGDVSVGDPILIDDGKVRLRVTKVDGPDVHTEVLVGGKVSDNKGINLPG
VAVSVPALSEKDIEDLRFALHLTVDFIALSFVRDAKDVEDVRRIMDEEGVHLPVIAKIEKPQAIENLDEVIAAFDGFMVA
RGDLGVECPLEDVPFLQKRIVEKARLNAKPVIVATQMLESMIGNPAPTRAEASDVANAVLDGADAVMLSGETSVGEYPVH
TVETMARIIAATEAHALAQPPGRAALAEIDWDPHTRGGVIAKAAEEVAERVGAKYVVAFTQSGDSARRMSRLRGPIPILA
FTPVGVVRSQLALTWGIETFLTQPVEHTDEMVRQVDDQLLRIGRVREGDLVVIIAGAPPGIPGSTNALRIHRMGDAINQV
APAYRRS

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=491, Percent_Identity=39.1038696537678, Blast_Score=305, Evalue=8e-83,
Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=38.4458077709611, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=38.4458077709611, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI10835121, Length=495, Percent_Identity=40.6060606060606, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI32967597, Length=495, Percent_Identity=40.6060606060606, Blast_Score=292, Evalue=6e-79,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=39.3728222996516, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=39.3728222996516, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=37.4468085106383, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=37.4468085106383, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=37.3831775700935, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1787965, Length=479, Percent_Identity=40.7098121085595, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=40.4166666666667, Blast_Score=306, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17544584, Length=481, Percent_Identity=39.2931392931393, Blast_Score=308, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=40.5349794238683, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=40.5349794238683, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=40.4123711340206, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17506831, Length=488, Percent_Identity=40.5737704918033, Blast_Score=286, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6319279, Length=482, Percent_Identity=36.9294605809129, Blast_Score=279, Evalue=6e-76,
Organism=Saccharomyces cerevisiae, GI6324923, Length=490, Percent_Identity=35.5102040816327, Blast_Score=256, Evalue=4e-69,
Organism=Drosophila melanogaster, GI28571814, Length=496, Percent_Identity=38.9112903225806, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24648964, Length=496, Percent_Identity=38.9112903225806, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24648966, Length=411, Percent_Identity=39.6593673965937, Blast_Score=272, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24581235, Length=489, Percent_Identity=32.3108384458078, Blast_Score=239, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24646914, Length=276, Percent_Identity=39.8550724637681, Blast_Score=183, Evalue=2e-46,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52492; Mature: 52492

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAKIVCTLGPAVANPRRIRELVYAGMDVARLNMSHGSHEDHAEAYRLVREASDASGHG
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCC
VGIFADLQGPKIRLERFANGPVVLTRGQAWTITTRDVPGDDRECGTTYKGLPGDVSVGDP
EEEEEECCCCEEEHEECCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCE
ILIDDGKVRLRVTKVDGPDVHTEVLVGGKVSDNKGINLPGVAVSVPALSEKDIEDLRFAL
EEEECCEEEEEEEECCCCCCCEEEEECCEECCCCCCCCCCCEEECCCCCCCCHHHHHHHH
HLTVDFIALSFVRDAKDVEDVRRIMDEEGVHLPVIAKIEKPQAIENLDEVIAAFDGFMVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHCCEEEE
RGDLGVECPLEDVPFLQKRIVEKARLNAKPVIVATQMLESMIGNPAPTRAEASDVANAVL
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
DGADAVMLSGETSVGEYPVHTVETMARIIAATEAHALAQPPGRAALAEIDWDPHTRGGVI
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCH
AKAAEEVAERVGAKYVVAFTQSGDSARRMSRLRGPIPILAFTPVGVVRSQLALTWGIETF
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCHHHH
LTQPVEHTDEMVRQVDDQLLRIGRVREGDLVVIIAGAPPGIPGSTNALRIHRMGDAINQV
HCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEEEEEHHHHHHHH
APAYRRS
CHHHHCC
>Mature Secondary Structure
MRRAKIVCTLGPAVANPRRIRELVYAGMDVARLNMSHGSHEDHAEAYRLVREASDASGHG
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCC
VGIFADLQGPKIRLERFANGPVVLTRGQAWTITTRDVPGDDRECGTTYKGLPGDVSVGDP
EEEEEECCCCEEEHEECCCCCEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCE
ILIDDGKVRLRVTKVDGPDVHTEVLVGGKVSDNKGINLPGVAVSVPALSEKDIEDLRFAL
EEEECCEEEEEEEECCCCCCCEEEEECCEECCCCCCCCCCCEEECCCCCCCCHHHHHHHH
HLTVDFIALSFVRDAKDVEDVRRIMDEEGVHLPVIAKIEKPQAIENLDEVIAAFDGFMVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHCCEEEE
RGDLGVECPLEDVPFLQKRIVEKARLNAKPVIVATQMLESMIGNPAPTRAEASDVANAVL
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
DGADAVMLSGETSVGEYPVHTVETMARIIAATEAHALAQPPGRAALAEIDWDPHTRGGVI
CCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCH
AKAAEEVAERVGAKYVVAFTQSGDSARRMSRLRGPIPILAFTPVGVVRSQLALTWGIETF
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCHHHH
LTQPVEHTDEMVRQVDDQLLRIGRVREGDLVVIIAGAPPGIPGSTNALRIHRMGDAINQV
HCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEEEEEHHHHHHHH
APAYRRS
CHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA