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The map label for this gene is livJ [C]

Identifier: 119717241

GI number: 119717241

Start: 3213538

End: 3214818

Strand: Reverse

Name: livJ [C]

Synonym: Noca_3017

Alternate gene names: 119717241

Gene position: 3214818-3213538 (Counterclockwise)

Preceding gene: 119717242

Following gene: 119717232

Centisome position: 64.48

GC content: 67.92

Gene sequence:

>1281_bases
ATGATTCGCTCCAGCCGCGCTCGCCGCGCAGCCATCACGCTCGCGGCATCCGCACTCGTCCTCACTGCTTGTGGCAGTGA
CGGCGGCTCCGACAGCAAGTCCGACGCTCCCGACGAGGGCACCTCCTCGTCGGCGCCGGCAACGACGGGTGACGGCGTGC
TCAAGATCGGCCAGCTGCTGCCCCAGACCGGTGACCTCGCCTACCTGGGCCCCCCCGAGTTCGCGGGCGTCGACCTGGCC
ATCAAGGAAATCAACGACGCGGGTGGCGTGCTCGGGAAGCCCGTCGAGAGCTTCAAGGCGGACTCCGGCGACGGTACGCC
GGATATCGCGGGCGCCTCCGTCGACTCGCTCCTCCAGGACAGCGTCGACGCCATCGTCGGTGCCGCCGCCTCCGGCGTGT
CGCTCTCGGTGATCGACAAGATCACCGGTGCCGGCGTCGTGCAGATCTCCCCGGCGAACACCGCCGCGGCGTTCGACACC
TACGACGACGGCGGCCTGTACTTCCGCACCGCGCCGTCCGACCGCCTGCAGGGCCAGGTGCTCGGCAACATGGCGGTCGA
GGACGGGTTCTCCAACGTCGCCGTGATGGCGCGCCAGGATGCGTACGGCGAGGGTCTGGCCGAGCAGGTCGACCAGACGC
TGAAGGAGCAGGGCGCCAACGTCGCGGCCCACATCCTCTACGCCGCCGACGCTCAGAACTTCACCGCAGAGGTCAACGAG
ATCGCGGCGGCCAAGCCCGACGCTCTCGTGCTGATCGCGTTCAACGAGACGACGAAGATCATCCCGCAGCTGATCGCCAA
GGGGATCGGCCCGCAGGACATCCAGCTCTACTTCGTCGACGGCAACATGGCCGACTACTCCGCCGAGTCCTTCGACCTGG
AGGGCGTCAAGGGCACCTTCCCGGCTCCGGCCGAGGTCGACGAGAGCTTCAACCAGCGGCTGCTCGAGGTGGACCCGAAG
CTGAAGGACTTCACCTACGGCCCGCAGTCCTACGACGCCACGATCCTCACCGCGCTCGCTGCGATCGCGGCCGGGGACGA
CTCCGGCGAGGCGATCGCCGGCGAGCTGGTCAACGTCTCCAAGGACGGCGAGGCCTGCACCACGTTCGCCGACTGCGCGA
AGCTGCTCGAGGACGGCCAGGACATCAACTACGAGGGTGTCTCCGGCCCGACCGACATGAACGACACCGGCAGCCCGAAC
GCTGCGACGATCGGCATCCAGGAGTACGCCAAGAACAACAAGTACTCGCAGATCGACTCGGTCTCCGGCGTCCTGGAGTG
A

Upstream 100 bases:

>100_bases
CTCTGGTCCCGGGTTCGACGCTAGTTTGCCGGGTATCGAGAGTCGATCTCCGGTCCCGCCCCGCGCGGACCGGAGGAAAG
CTAGTCCAGACGGAGGCTTC

Downstream 100 bases:

>100_bases
CCCGGCGCTGACCGACGCACAAGACGACGGGCCCGACCTCCGCGGAGGTCGGGCCCGTTCGTCGTTGTGCCCGGCTCAGG
CCTGGGCGAGGGTGCCGAGG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MIRSSRARRAAITLAASALVLTACGSDGGSDSKSDAPDEGTSSSAPATTGDGVLKIGQLLPQTGDLAYLGPPEFAGVDLA
IKEINDAGGVLGKPVESFKADSGDGTPDIAGASVDSLLQDSVDAIVGAAASGVSLSVIDKITGAGVVQISPANTAAAFDT
YDDGGLYFRTAPSDRLQGQVLGNMAVEDGFSNVAVMARQDAYGEGLAEQVDQTLKEQGANVAAHILYAADAQNFTAEVNE
IAAAKPDALVLIAFNETTKIIPQLIAKGIGPQDIQLYFVDGNMADYSAESFDLEGVKGTFPAPAEVDESFNQRLLEVDPK
LKDFTYGPQSYDATILTALAAIAAGDDSGEAIAGELVNVSKDGEACTTFADCAKLLEDGQDINYEGVSGPTDMNDTGSPN
AATIGIQEYAKNNKYSQIDSVSGVLE

Sequences:

>Translated_426_residues
MIRSSRARRAAITLAASALVLTACGSDGGSDSKSDAPDEGTSSSAPATTGDGVLKIGQLLPQTGDLAYLGPPEFAGVDLA
IKEINDAGGVLGKPVESFKADSGDGTPDIAGASVDSLLQDSVDAIVGAAASGVSLSVIDKITGAGVVQISPANTAAAFDT
YDDGGLYFRTAPSDRLQGQVLGNMAVEDGFSNVAVMARQDAYGEGLAEQVDQTLKEQGANVAAHILYAADAQNFTAEVNE
IAAAKPDALVLIAFNETTKIIPQLIAKGIGPQDIQLYFVDGNMADYSAESFDLEGVKGTFPAPAEVDESFNQRLLEVDPK
LKDFTYGPQSYDATILTALAAIAAGDDSGEAIAGELVNVSKDGEACTTFADCAKLLEDGQDINYEGVSGPTDMNDTGSPN
AATIGIQEYAKNNKYSQIDSVSGVLE
>Mature_426_residues
MIRSSRARRAAITLAASALVLTACGSDGGSDSKSDAPDEGTSSSAPATTGDGVLKIGQLLPQTGDLAYLGPPEFAGVDLA
IKEINDAGGVLGKPVESFKADSGDGTPDIAGASVDSLLQDSVDAIVGAAASGVSLSVIDKITGAGVVQISPANTAAAFDT
YDDGGLYFRTAPSDRLQGQVLGNMAVEDGFSNVAVMARQDAYGEGLAEQVDQTLKEQGANVAAHILYAADAQNFTAEVNE
IAAAKPDALVLIAFNETTKIIPQLIAKGIGPQDIQLYFVDGNMADYSAESFDLEGVKGTFPAPAEVDESFNQRLLEVDPK
LKDFTYGPQSYDATILTALAAIAAGDDSGEAIAGELVNVSKDGEACTTFADCAKLLEDGQDINYEGVSGPTDMNDTGSPN
AATIGIQEYAKNNKYSQIDSVSGVLE

Specific function: This Protein Is A Component Of The Leucine, Isoleucine, Valine, (Threonine) Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids. [C]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 43756; Mature: 43756

Theoretical pI: Translated: 3.82; Mature: 3.82

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRSSRARRAAITLAASALVLTACGSDGGSDSKSDAPDEGTSSSAPATTGDGVLKIGQLL
CCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
PQTGDLAYLGPPEFAGVDLAIKEINDAGGVLGKPVESFKADSGDGTPDIAGASVDSLLQD
CCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHH
SVDAIVGAAASGVSLSVIDKITGAGVVQISPANTAAAFDTYDDGGLYFRTAPSDRLQGQV
HHHHHHHHHCCCCEEEEHHHHCCCCEEEECCCCCCEEECCCCCCCEEEEECCCHHHCHHH
LGNMAVEDGFSNVAVMARQDAYGEGLAEQVDQTLKEQGANVAAHILYAADAQNFTAEVNE
HHHHHHHCCCCCEEEEEECCHHCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHH
IAAAKPDALVLIAFNETTKIIPQLIAKGIGPQDIQLYFVDGNMADYSAESFDLEGVKGTF
HHCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
PAPAEVDESFNQRLLEVDPKLKDFTYGPQSYDATILTALAAIAAGDDSGEAIAGELVNVS
CCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHEECC
KDGEACTTFADCAKLLEDGQDINYEGVSGPTDMNDTGSPNAATIGIQEYAKNNKYSQIDS
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCHHHHH
VSGVLE
HHHHCC
>Mature Secondary Structure
MIRSSRARRAAITLAASALVLTACGSDGGSDSKSDAPDEGTSSSAPATTGDGVLKIGQLL
CCCCCCHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHC
PQTGDLAYLGPPEFAGVDLAIKEINDAGGVLGKPVESFKADSGDGTPDIAGASVDSLLQD
CCCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHH
SVDAIVGAAASGVSLSVIDKITGAGVVQISPANTAAAFDTYDDGGLYFRTAPSDRLQGQV
HHHHHHHHHCCCCEEEEHHHHCCCCEEEECCCCCCEEECCCCCCCEEEEECCCHHHCHHH
LGNMAVEDGFSNVAVMARQDAYGEGLAEQVDQTLKEQGANVAAHILYAADAQNFTAEVNE
HHHHHHHCCCCCEEEEEECCHHCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHH
IAAAKPDALVLIAFNETTKIIPQLIAKGIGPQDIQLYFVDGNMADYSAESFDLEGVKGTF
HHCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
PAPAEVDESFNQRLLEVDPKLKDFTYGPQSYDATILTALAAIAAGDDSGEAIAGELVNVS
CCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHEECC
KDGEACTTFADCAKLLEDGQDINYEGVSGPTDMNDTGSPNAATIGIQEYAKNNKYSQIDS
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCCCHHHHH
VSGVLE
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]