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The map label for this gene is ygfK [H]

Identifier: 119716585

GI number: 119716585

Start: 2513583

End: 2515217

Strand: Reverse

Name: ygfK [H]

Synonym: Noca_2358

Alternate gene names: 119716585

Gene position: 2515217-2513583 (Counterclockwise)

Preceding gene: 119716586

Following gene: 119716580

Centisome position: 50.45

GC content: 73.15

Gene sequence:

>1635_bases
ATGGACCACCCGTTCGCCATCACCCTGGACGTCGGCTCGAGCCGGGCCAACAAGACCGGCTCCTGGCGTACCGAGCGTCC
CGTCTACGTCCACAACCAGCCGCCGTGCAACCGGGCCTGCCCCGCCGGCGAGGACATCCGCACCTGGCTGTACGACGCCG
AGGAGGGCGGGGACGCCTACAAGCGGGCGTGGCGCCGGATCATGGAGGTCAACCCGTTCCCGGCGGTCATGGGCCGGGTC
TGCTACCACCCGTGCGAGACGGGCTGCAACCGCGGCCAGCTCGACGAGGCCGTCGGCATCAACTCCGTCGAGCGCTTCCT
CGGCGACGAGGCGATCCGCTGCGGCTGGCAGGTCACCGTCGACCGCCCGTCGAGCGGGCTGCGGGTGCTCGTCGTCGGCG
CCGGCCCCTCGGGGCTCTCGGCGGCCTACCAGCTCGCCCGGCTCGGGCACGCCGTGACGGTCCTGGAGTCGGCCCCGAAG
CCCGGCGGCATGATGCGCTACGGCATCCCGGAGTACCGCCTCCCCCGCGCGGTCCTGGACGCCGAGATCCAGCGGATCCT
CGACCTGGGCGTGCGCCTGCAGGTGGGCGTCACCGTGACCGACCTCGACGCGACCATGCGCGAGGGGCGGTACGACGCGG
CCTTCCTGGCGGTCGGCGCCCAGCTCGGCAAGCGCGCCTACCTGCCGGCCGGCGCGGCCTCCCACGTCGTCGACGCGGTC
GCGCTGCTGCACCAGCTCGGCGAGGGCGAGCGTCCGATGCTCGGGCGCCGGGTCGCCATCTACGGCGGCGGCAACACCGC
CGTCGACGCGGCCCGCACCGCCAAGCGGCTCGGCGCCGAGGAGTCGCTGGTCGTCTACCGGCGCACCCGCGACCGGATGC
CCGCGCACGAGGCCGAGGTCCGCGAGGCCGAGGAGGAGGGCGTCCGGTTCACCTGGCTGTCCTCGATCACCCAGGTCGAG
GAGGGCTCCTTGACCATCGAGCGGATGGAGCTCGACGAGACCGGGTTCCCACAGCCGACCGGGGTCACCGAGCAGCTGCC
GGCCGACTCACTCGTGCTCGCGCTCGGCCAGGACACCGATCTGCGCTTCCTGGACGGGGTCGCCGGCATCGAGGTCGACC
ACGGCGTGGTGTCTGTCGACGACCAGATGATGACCGGTCGCCCGGGCATCTTCGCCGGCGGCGACATGGTGCCCGACCAG
CGCTCGGCGACGGTCGGCATCGGCCACGGCCGACGGGCGGCGATGGGGGTGGATGCCTGGCTGCGCGACCGGACGGCGCC
GCGGCCCGCGGAGCCGGCTCCCGCCGAGTTCGACGCGCTGAACACCTGGTACTTCGCCGACGCGCCCCGCGCGCACCGGC
CGCTGCTCGAAGCGGTGCGCCGGTCGTCGACCTTCGACGAGGTCGTGGAGTCCCTCGACGCCGACAGCGCCCTCTACGAG
GCACGGCGCTGCATGTCCTGCGGCAACTGCTTCTCCTGCGACAACTGCTTCGGCGTGTGCCCCGACAACGCCATCACCAA
GACCGGCGACCCGGACACGCCGTACCTGATCGACCTCGACTACTGCAAGGGCTGCGGGCTGTGCGCCGCGGAGTGCCCGG
CCGGGGCGATCCGGATGGCACCCGAGGAGATCTAG

Upstream 100 bases:

>100_bases
GCCCGTCCCGATCTCGTCGCGCGGCTCCAGGCCGGCGCCGACCGCAACATCGCCCGCTTCGGCCTCCTCGAGGCCGAGCC
CCGAACCGGGAGCGACTGAC

Downstream 100 bases:

>100_bases
CGGACCCGGGTTCGCCCGGCCGGGACGGGGCTCAGCCTCGGTCAGCTGCACGCCTCGACCGGCGGGCCGCCGTCGCGCAG
CCACCGGTCGTAGCGGGCGG

Product: putative glutamate synthase (NADPH) small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MDHPFAITLDVGSSRANKTGSWRTERPVYVHNQPPCNRACPAGEDIRTWLYDAEEGGDAYKRAWRRIMEVNPFPAVMGRV
CYHPCETGCNRGQLDEAVGINSVERFLGDEAIRCGWQVTVDRPSSGLRVLVVGAGPSGLSAAYQLARLGHAVTVLESAPK
PGGMMRYGIPEYRLPRAVLDAEIQRILDLGVRLQVGVTVTDLDATMREGRYDAAFLAVGAQLGKRAYLPAGAASHVVDAV
ALLHQLGEGERPMLGRRVAIYGGGNTAVDAARTAKRLGAEESLVVYRRTRDRMPAHEAEVREAEEEGVRFTWLSSITQVE
EGSLTIERMELDETGFPQPTGVTEQLPADSLVLALGQDTDLRFLDGVAGIEVDHGVVSVDDQMMTGRPGIFAGGDMVPDQ
RSATVGIGHGRRAAMGVDAWLRDRTAPRPAEPAPAEFDALNTWYFADAPRAHRPLLEAVRRSSTFDEVVESLDADSALYE
ARRCMSCGNCFSCDNCFGVCPDNAITKTGDPDTPYLIDLDYCKGCGLCAAECPAGAIRMAPEEI

Sequences:

>Translated_544_residues
MDHPFAITLDVGSSRANKTGSWRTERPVYVHNQPPCNRACPAGEDIRTWLYDAEEGGDAYKRAWRRIMEVNPFPAVMGRV
CYHPCETGCNRGQLDEAVGINSVERFLGDEAIRCGWQVTVDRPSSGLRVLVVGAGPSGLSAAYQLARLGHAVTVLESAPK
PGGMMRYGIPEYRLPRAVLDAEIQRILDLGVRLQVGVTVTDLDATMREGRYDAAFLAVGAQLGKRAYLPAGAASHVVDAV
ALLHQLGEGERPMLGRRVAIYGGGNTAVDAARTAKRLGAEESLVVYRRTRDRMPAHEAEVREAEEEGVRFTWLSSITQVE
EGSLTIERMELDETGFPQPTGVTEQLPADSLVLALGQDTDLRFLDGVAGIEVDHGVVSVDDQMMTGRPGIFAGGDMVPDQ
RSATVGIGHGRRAAMGVDAWLRDRTAPRPAEPAPAEFDALNTWYFADAPRAHRPLLEAVRRSSTFDEVVESLDADSALYE
ARRCMSCGNCFSCDNCFGVCPDNAITKTGDPDTPYLIDLDYCKGCGLCAAECPAGAIRMAPEEI
>Mature_544_residues
MDHPFAITLDVGSSRANKTGSWRTERPVYVHNQPPCNRACPAGEDIRTWLYDAEEGGDAYKRAWRRIMEVNPFPAVMGRV
CYHPCETGCNRGQLDEAVGINSVERFLGDEAIRCGWQVTVDRPSSGLRVLVVGAGPSGLSAAYQLARLGHAVTVLESAPK
PGGMMRYGIPEYRLPRAVLDAEIQRILDLGVRLQVGVTVTDLDATMREGRYDAAFLAVGAQLGKRAYLPAGAASHVVDAV
ALLHQLGEGERPMLGRRVAIYGGGNTAVDAARTAKRLGAEESLVVYRRTRDRMPAHEAEVREAEEEGVRFTWLSSITQVE
EGSLTIERMELDETGFPQPTGVTEQLPADSLVLALGQDTDLRFLDGVAGIEVDHGVVSVDDQMMTGRPGIFAGGDMVPDQ
RSATVGIGHGRRAAMGVDAWLRDRTAPRPAEPAPAEFDALNTWYFADAPRAHRPLLEAVRRSSTFDEVVESLDADSALYE
ARRCMSCGNCFSCDNCFGVCPDNAITKTGDPDTPYLIDLDYCKGCGLCAAECPAGAIRMAPEEI

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=428, Percent_Identity=26.1682242990654, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1789243, Length=518, Percent_Identity=31.4671814671815, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1789606, Length=424, Percent_Identity=31.3679245283019, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87082180, Length=431, Percent_Identity=32.2505800464037, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI1788468, Length=398, Percent_Identity=31.9095477386935, Blast_Score=165, Evalue=8e-42,
Organism=Escherichia coli, GI1788811, Length=421, Percent_Identity=30.8788598574822, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17570289, Length=268, Percent_Identity=32.089552238806, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71984108, Length=438, Percent_Identity=25.5707762557078, Blast_Score=98, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6320030, Length=428, Percent_Identity=27.5700934579439, Blast_Score=124, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24665547, Length=432, Percent_Identity=29.6296296296296, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24665543, Length=432, Percent_Identity=29.6296296296296, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28574881, Length=432, Percent_Identity=29.6296296296296, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24665539, Length=432, Percent_Identity=29.6296296296296, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24640763, Length=434, Percent_Identity=27.1889400921659, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI18858217, Length=434, Percent_Identity=27.1889400921659, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR017701 [H]

Pfam domain/function: PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 58984; Mature: 58984

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHPFAITLDVGSSRANKTGSWRTERPVYVHNQPPCNRACPAGEDIRTWLYDAEEGGDAY
CCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHEECCCCCCHHH
KRAWRRIMEVNPFPAVMGRVCYHPCETGCNRGQLDEAVGINSVERFLGDEAIRCGWQVTV
HHHHHHHHCCCCHHHHHHHHHHCHHHCCCCCCCCHHHHCHHHHHHHHCCHHHHCCEEEEE
DRPSSGLRVLVVGAGPSGLSAAYQLARLGHAVTVLESAPKPGGMMRYGIPEYRLPRAVLD
ECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHCCHHHHH
AEIQRILDLGVRLQVGVTVTDLDATMREGRYDAAFLAVGAQLGKRAYLPAGAASHVVDAV
HHHHHHHHCCCEEEEEEEEECCHHHHHCCCCCHHHHHHHHHHCCEECCCCCHHHHHHHHH
ALLHQLGEGERPMLGRRVAIYGGGNTAVDAARTAKRLGAEESLVVYRRTRDRMPAHEAEV
HHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECHHCCCCCHHHHH
REAEEEGVRFTWLSSITQVEEGSLTIERMELDETGFPQPTGVTEQLPADSLVLALGQDTD
HHHHHCCCEEEEHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHCCCCCEEEEECCCCC
LRFLDGVAGIEVDHGVVSVDDQMMTGRPGIFAGGDMVPDQRSATVGIGHGRRAAMGVDAW
CHHHCCCCCCEECCCEEEECCHHHCCCCCEEECCCCCCCCCCCEEECCCCCCHHHCHHHH
LRDRTAPRPAEPAPAEFDALNTWYFADAPRAHRPLLEAVRRSSTFDEVVESLDADSALYE
HHCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHH
ARRCMSCGNCFSCDNCFGVCPDNAITKTGDPDTPYLIDLDYCKGCGLCAAECPAGAIRMA
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCHHCCCCCCCCCC
PEEI
CCCC
>Mature Secondary Structure
MDHPFAITLDVGSSRANKTGSWRTERPVYVHNQPPCNRACPAGEDIRTWLYDAEEGGDAY
CCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHEECCCCCCHHH
KRAWRRIMEVNPFPAVMGRVCYHPCETGCNRGQLDEAVGINSVERFLGDEAIRCGWQVTV
HHHHHHHHCCCCHHHHHHHHHHCHHHCCCCCCCCHHHHCHHHHHHHHCCHHHHCCEEEEE
DRPSSGLRVLVVGAGPSGLSAAYQLARLGHAVTVLESAPKPGGMMRYGIPEYRLPRAVLD
ECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHCCHHHHH
AEIQRILDLGVRLQVGVTVTDLDATMREGRYDAAFLAVGAQLGKRAYLPAGAASHVVDAV
HHHHHHHHCCCEEEEEEEEECCHHHHHCCCCCHHHHHHHHHHCCEECCCCCHHHHHHHHH
ALLHQLGEGERPMLGRRVAIYGGGNTAVDAARTAKRLGAEESLVVYRRTRDRMPAHEAEV
HHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECHHCCCCCHHHHH
REAEEEGVRFTWLSSITQVEEGSLTIERMELDETGFPQPTGVTEQLPADSLVLALGQDTD
HHHHHCCCEEEEHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHCCCCCEEEEECCCCC
LRFLDGVAGIEVDHGVVSVDDQMMTGRPGIFAGGDMVPDQRSATVGIGHGRRAAMGVDAW
CHHHCCCCCCEECCCEEEECCHHHCCCCCEEECCCCCCCCCCCEEECCCCCCHHHCHHHH
LRDRTAPRPAEPAPAEFDALNTWYFADAPRAHRPLLEAVRRSSTFDEVVESLDADSALYE
HHCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHH
ARRCMSCGNCFSCDNCFGVCPDNAITKTGDPDTPYLIDLDYCKGCGLCAAECPAGAIRMA
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCHHCCCCCCCCCC
PEEI
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]