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The map label for this gene is eco47IIM [H]

Identifier: 119716511

GI number: 119716511

Start: 2433741

End: 2434940

Strand: Reverse

Name: eco47IIM [H]

Synonym: Noca_2284

Alternate gene names: 119716511

Gene position: 2434940-2433741 (Counterclockwise)

Preceding gene: 119716512

Following gene: 119716510

Centisome position: 48.84

GC content: 65.08

Gene sequence:

>1200_bases
ATGGTCCACGACGCTCCGGAGGCAGCAGACGACGCCGACTCTGGCTCTTGCATCGAACTCTTTTCCGGTGGTGGTGGCCT
CGCGATGGCACTGCACGAGGCTGGCTTTCGCCACCTTCTGCTCAACGAGCTGAACAAGCGTGCTTGCGCGACCCTCAGGG
CCAACAACGCCGTGGACTACCTGCCCGACGAGACACCTCCCGCGACGCTCGCCGACCCGTGGCCCCTCATCGAAGGAGGG
ATTGGGGAAGTCGACTTCACGCCGTTCCTCGGAGACGTCGATGTCGTCGCTGGCGGCGTACCTTGCCAGCCGTTCAGCCT
TGGCGGGGCCCACAAGGGTCACCTCGACGAACGCAACCTGTGGCCTGAGTTCAACCGTTGTGTCCGGGAAACACGACCAC
TGGTCATCCTCGCCGAGAACGTGCGGGGCCTCCTCCGGCCCTCCTTCGAGCCCTATTGGGATTACATCCGCCGAGAACTG
GCTGCCCCATTCGAGCAACGCGTCGACGGGGAACCATGGGCCGATCATGACCGCCGTCTGGTCAAGGCGTTGCGCGGTGG
CGGCGGCGATCCCACCGAGCGATACGACATCGCGTTCAAGCTGGTCAACGCCGCCGACTACGGCGTTCCGCAGAATCGAT
GGCGAGTTGTGCTGGTCGGTTTCCGCAAGGATCTAGGGATCTCGTGGAGCTTCCCCGACCCCACGCACAGTGCCGGCGCT
CTTCTGCGCGCCCAACTGTCCGGAGAGTACTCAGATCGGCATCCCCACGCGCCGATCAAAGAGCATCCCGGCGTCACGCC
ACCGGAGGACGGTCTACGGCCATGGAAGACCCTGAGGGACGCCATCCACGACCTGCCCGAACCGGTAGAGCGACAGGACA
CGCCGGGCTACATCCACCACATCGGCTGGCCAGGCGCTCGTGAGTACCCCGGGCACACCGCCAACGTGCTCGACAGGCCG
GCCAAGACCGTCAAGGCAGGCGTACACGGCGTTCCTGGCGGTGAGTCGGTTCTCCGGCGTGATGACGGGAGCATCCGCTA
CCTGACTGTTCGCGAAGTCGCGCGCATCATGACGTTCCCTGACGATTGGCGGCTCGAGGGTCCGCGGGGCGAGCAAATGC
GCCAACTCGGGAATGCCGTCCCCGTCCGTCTCGGTGCAGTGATGGGTCGCGAGATCGCGAAGGTTCTGCGGGAACGATGA

Upstream 100 bases:

>100_bases
CTCGTTCAACCAGCTAGGTGCTGTCCGGGTCGCACGATGCCCTCTTAAAGACGCGTGTTCACGCCCTTCGTGTCGGAGGT
CCGCGGTAGCGTCCCGCGGT

Downstream 100 bases:

>100_bases
CCAAGACCAAAACGCAAGTAGTCCAGCCGCGCGATCCGGCCGTGACCAGTCGCATCATGAGCGCGGTTCGCGCGAAGGAC
ACCAAGCCCGAGCTCCTCCT

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.Eco47II; Cytosine-specific methyltransferase Eco47II [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MVHDAPEAADDADSGSCIELFSGGGGLAMALHEAGFRHLLLNELNKRACATLRANNAVDYLPDETPPATLADPWPLIEGG
IGEVDFTPFLGDVDVVAGGVPCQPFSLGGAHKGHLDERNLWPEFNRCVRETRPLVILAENVRGLLRPSFEPYWDYIRREL
AAPFEQRVDGEPWADHDRRLVKALRGGGGDPTERYDIAFKLVNAADYGVPQNRWRVVLVGFRKDLGISWSFPDPTHSAGA
LLRAQLSGEYSDRHPHAPIKEHPGVTPPEDGLRPWKTLRDAIHDLPEPVERQDTPGYIHHIGWPGAREYPGHTANVLDRP
AKTVKAGVHGVPGGESVLRRDDGSIRYLTVREVARIMTFPDDWRLEGPRGEQMRQLGNAVPVRLGAVMGREIAKVLRER

Sequences:

>Translated_399_residues
MVHDAPEAADDADSGSCIELFSGGGGLAMALHEAGFRHLLLNELNKRACATLRANNAVDYLPDETPPATLADPWPLIEGG
IGEVDFTPFLGDVDVVAGGVPCQPFSLGGAHKGHLDERNLWPEFNRCVRETRPLVILAENVRGLLRPSFEPYWDYIRREL
AAPFEQRVDGEPWADHDRRLVKALRGGGGDPTERYDIAFKLVNAADYGVPQNRWRVVLVGFRKDLGISWSFPDPTHSAGA
LLRAQLSGEYSDRHPHAPIKEHPGVTPPEDGLRPWKTLRDAIHDLPEPVERQDTPGYIHHIGWPGAREYPGHTANVLDRP
AKTVKAGVHGVPGGESVLRRDDGSIRYLTVREVARIMTFPDDWRLEGPRGEQMRQLGNAVPVRLGAVMGREIAKVLRER
>Mature_399_residues
MVHDAPEAADDADSGSCIELFSGGGGLAMALHEAGFRHLLLNELNKRACATLRANNAVDYLPDETPPATLADPWPLIEGG
IGEVDFTPFLGDVDVVAGGVPCQPFSLGGAHKGHLDERNLWPEFNRCVRETRPLVILAENVRGLLRPSFEPYWDYIRREL
AAPFEQRVDGEPWADHDRRLVKALRGGGGDPTERYDIAFKLVNAADYGVPQNRWRVVLVGFRKDLGISWSFPDPTHSAGA
LLRAQLSGEYSDRHPHAPIKEHPGVTPPEDGLRPWKTLRDAIHDLPEPVERQDTPGYIHHIGWPGAREYPGHTANVLDRP
AKTVKAGVHGVPGGESVLRRDDGSIRYLTVREVARIMTFPDDWRLEGPRGEQMRQLGNAVPVRLGAVMGREIAKVLRER

Specific function: This methylase recognizes the double-stranded sequence GGNCC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the Eco47II endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525
- InterPro:   IPR009061
- InterPro:   IPR000551 [H]

Pfam domain/function: PF00145 DNA_methylase; PF00376 MerR [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 44164; Mature: 44164

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHDAPEAADDADSGSCIELFSGGGGLAMALHEAGFRHLLLNELNKRACATLRANNAVDY
CCCCCCCCCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LPDETPPATLADPWPLIEGGIGEVDFTPFLGDVDVVAGGVPCQPFSLGGAHKGHLDERNL
CCCCCCCCCCCCCCCHHCCCCCCEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
WPEFNRCVRETRPLVILAENVRGLLRPSFEPYWDYIRRELAAPFEQRVDGEPWADHDRRL
CHHHHHHHHHCCCEEEEECCHHHHHCCCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHH
VKALRGGGGDPTERYDIAFKLVNAADYGVPQNRWRVVLVGFRKDLGISWSFPDPTHSAGA
HHHHHCCCCCCCHHHEEEEEEECHHHCCCCCCCCEEEEEEECCCCCEEECCCCCCCHHHH
LLRAQLSGEYSDRHPHAPIKEHPGVTPPEDGLRPWKTLRDAIHDLPEPVERQDTPGYIHH
HHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCCEEEE
IGWPGAREYPGHTANVLDRPAKTVKAGVHGVPGGESVLRRDDGSIRYLTVREVARIMTFP
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHCCC
DDWRLEGPRGEQMRQLGNAVPVRLGAVMGREIAKVLRER
CCCEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVHDAPEAADDADSGSCIELFSGGGGLAMALHEAGFRHLLLNELNKRACATLRANNAVDY
CCCCCCCCCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LPDETPPATLADPWPLIEGGIGEVDFTPFLGDVDVVAGGVPCQPFSLGGAHKGHLDERNL
CCCCCCCCCCCCCCCHHCCCCCCEECCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCC
WPEFNRCVRETRPLVILAENVRGLLRPSFEPYWDYIRRELAAPFEQRVDGEPWADHDRRL
CHHHHHHHHHCCCEEEEECCHHHHHCCCCCHHHHHHHHHHCCCHHHHCCCCCCCHHHHHH
VKALRGGGGDPTERYDIAFKLVNAADYGVPQNRWRVVLVGFRKDLGISWSFPDPTHSAGA
HHHHHCCCCCCCHHHEEEEEEECHHHCCCCCCCCEEEEEEECCCCCEEECCCCCCCHHHH
LLRAQLSGEYSDRHPHAPIKEHPGVTPPEDGLRPWKTLRDAIHDLPEPVERQDTPGYIHH
HHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCCEEEE
IGWPGAREYPGHTANVLDRPAKTVKAGVHGVPGGESVLRRDDGSIRYLTVREVARIMTFP
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEHHHHHHHHCCC
DDWRLEGPRGEQMRQLGNAVPVRLGAVMGREIAKVLRER
CCCEECCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7607524 [H]