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The map label for this gene is vsr [H]

Identifier: 119716510

GI number: 119716510

Start: 2433283

End: 2433744

Strand: Reverse

Name: vsr [H]

Synonym: Noca_2283

Alternate gene names: 119716510

Gene position: 2433744-2433283 (Counterclockwise)

Preceding gene: 119716511

Following gene: 119716509

Centisome position: 48.81

GC content: 66.88

Gene sequence:

>462_bases
ATGACCAAGACCAAAACGCAAGTAGTCCAGCCGCGCGATCCGGCCGTGACCAGTCGCATCATGAGCGCGGTTCGCGCGAA
GGACACCAAGCCCGAGCTCCTCCTCCGCCGCGCCGTGCATGCGCGCGGCGGCCGATACCGCCTGCACGCGCCGGACGTGC
CGGGGCGCCCAGACCTGGTGGTTCGCGGCAAGAAGATCGCCATCTTCGTCGACGGCGACCTTTGGCACGGCAACCCCGCA
GAGTGGCGACGTCGGGGCAAGGACGATCTGGCTGGTTTGTTCCCCACCCGGACCCAGTGGTGGGTGGACAAGATCGAACG
GAACGTCCGACGAGATCAGGACGTCGACCGAGAGCTAGCCGCACGGGGATGGAGGGTGCTGCGCCTTTGGGCGAGCGACA
TCATCGCCGATCCAGACGCCGCAGCCGACCGCGTGATCATCGCCCTGCGGGAAGACTCATGA

Upstream 100 bases:

>100_bases
AGGGTCCGCGGGGCGAGCAAATGCGCCAACTCGGGAATGCCGTCCCCGTCCGTCTCGGTGCAGTGATGGGTCGCGAGATC
GCGAAGGTTCTGCGGGAACG

Downstream 100 bases:

>100_bases
GCCGACGCGTCCGGGCAACCAAGCCCGACCCCGCCCTGTATCTGCCCACGAAGGGACTGACAAGCAGCGAACGCGCGGCG
GAGCAGGACATGGCTGCCGG

Product: T/G mismatch-specific endonuclease

Products: DNA [C]

Alternate protein names: V.NmeDIP [H]

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MTKTKTQVVQPRDPAVTSRIMSAVRAKDTKPELLLRRAVHARGGRYRLHAPDVPGRPDLVVRGKKIAIFVDGDLWHGNPA
EWRRRGKDDLAGLFPTRTQWWVDKIERNVRRDQDVDRELAARGWRVLRLWASDIIADPDAAADRVIIALREDS

Sequences:

>Translated_153_residues
MTKTKTQVVQPRDPAVTSRIMSAVRAKDTKPELLLRRAVHARGGRYRLHAPDVPGRPDLVVRGKKIAIFVDGDLWHGNPA
EWRRRGKDDLAGLFPTRTQWWVDKIERNVRRDQDVDRELAARGWRVLRLWASDIIADPDAAADRVIIALREDS
>Mature_152_residues
TKTKTQVVQPRDPAVTSRIMSAVRAKDTKPELLLRRAVHARGGRYRLHAPDVPGRPDLVVRGKKIAIFVDGDLWHGNPAE
WRRRGKDDLAGLFPTRTQWWVDKIERNVRRDQDVDRELAARGWRVLRLWASDIIADPDAAADRVIIALREDS

Specific function: May nick NmeDIP sequences that contain T:G mispairs resulting from m5C-deamination [H]

COG id: COG3727

COG function: function code L; DNA G:T-mismatch repair endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vsr family [H]

Homologues:

Organism=Escherichia coli, GI1788270, Length=124, Percent_Identity=34.6774193548387, Blast_Score=70, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004603
- InterPro:   IPR011335 [H]

Pfam domain/function: PF03852 Vsr [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 17530; Mature: 17399

Theoretical pI: Translated: 11.24; Mature: 11.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTKTQVVQPRDPAVTSRIMSAVRAKDTKPELLLRRAVHARGGRYRLHAPDVPGRPDLV
CCCCCCCEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEE
VRGKKIAIFVDGDLWHGNPAEWRRRGKDDLAGLFPTRTQWWVDKIERNVRRDQDVDRELA
EECCEEEEEECCCCCCCCHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
ARGWRVLRLWASDIIADPDAAADRVIIALREDS
HCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
TKTKTQVVQPRDPAVTSRIMSAVRAKDTKPELLLRRAVHARGGRYRLHAPDVPGRPDLV
CCCCCCEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCEE
VRGKKIAIFVDGDLWHGNPAEWRRRGKDDLAGLFPTRTQWWVDKIERNVRRDQDVDRELA
EECCEEEEEECCCCCCCCHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHH
ARGWRVLRLWASDIIADPDAAADRVIIALREDS
HCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: DNA mismatch endonuclease vsr specifically recognizes a T-G mismatch in a special context related to the dcm methylation sequence and makes an incision 5' to the mismatched T residue [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10671450 [H]