Click here to switch to the map view.

The map label for this gene is greA [H]

Identifier: 119715224

GI number: 119715224

Start: 1029313

End: 1029816

Strand: Reverse

Name: greA [H]

Synonym: Noca_0980

Alternate gene names: 119715224

Gene position: 1029816-1029313 (Counterclockwise)

Preceding gene: 119715227

Following gene: 119715219

Centisome position: 20.65

GC content: 70.63

Gene sequence:

>504_bases
ATGACACAGGCAGCACAGCCGGGCACCGTCTGGCTGACCCAGGACGCCTACGACAAGCTGCGCGCCGAGCTGGAGAGCCT
CAAGGGCCCGGTCCGCCAGGAGATCATCGCGCGCATCAGCTCCGCTCGCGACGAGGGCGACCTGAAGGAGAACGGCGCCT
ACCACGCCGCCCGCGAGGAGCAGGGCAAGGTCGAGGGGCGGATCCGCCAGCTCGAGGACGTGCTGCGCCGCGCCGAGGTC
GGCGAGACCCCGCCGAACGACGGCGTCGTCGAGCCCGGGATGGTCGTCACCTACCGGTTCGTCGGCGACGCCGAGGACGA
GGTCGAGACCTTCCTGCTCGGCGCTCGCGAGATCGAGCCCGAGGGGCTCACCGTCTACTCCCCCCAGTCCCCGCTCGGCT
CGGCCATCAACGGCAAGGCGAAGGGCGACACCGTCAGCTACCTCGCGCCCAACGGCAAGGAGCTCCAGGTCGTCATCGTC
GACGCCGTCCCCTACGAGGGGTGA

Upstream 100 bases:

>100_bases
GCGTATTCGCACACGCCCACACCCGGACGTTCCGGGGGGACATCCCCTGACCCGGCGGACGAGCTGGTCCGTCGTACCTC
GAAGCAAGCAGGAGTACCCG

Downstream 100 bases:

>100_bases
GCGACCTCGGCTACTCGTACACGCGGTAGCCGCGCTCGCGCAGGCGCGCCAGCACCTGCGCGGTGTGCGGCTCCCCGCGG
GTCTCGAGCTGGACGTGCAC

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MTQAAQPGTVWLTQDAYDKLRAELESLKGPVRQEIIARISSARDEGDLKENGAYHAAREEQGKVEGRIRQLEDVLRRAEV
GETPPNDGVVEPGMVVTYRFVGDAEDEVETFLLGAREIEPEGLTVYSPQSPLGSAINGKAKGDTVSYLAPNGKELQVVIV
DAVPYEG

Sequences:

>Translated_167_residues
MTQAAQPGTVWLTQDAYDKLRAELESLKGPVRQEIIARISSARDEGDLKENGAYHAAREEQGKVEGRIRQLEDVLRRAEV
GETPPNDGVVEPGMVVTYRFVGDAEDEVETFLLGAREIEPEGLTVYSPQSPLGSAINGKAKGDTVSYLAPNGKELQVVIV
DAVPYEG
>Mature_166_residues
TQAAQPGTVWLTQDAYDKLRAELESLKGPVRQEIIARISSARDEGDLKENGAYHAAREEQGKVEGRIRQLEDVLRRAEVG
ETPPNDGVVEPGMVVTYRFVGDAEDEVETFLLGAREIEPEGLTVYSPQSPLGSAINGKAKGDTVSYLAPNGKELQVVIVD
AVPYEG

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=164, Percent_Identity=39.0243902439024, Blast_Score=88, Evalue=3e-19,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 18152; Mature: 18021

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS00829 GREAB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAAQPGTVWLTQDAYDKLRAELESLKGPVRQEIIARISSARDEGDLKENGAYHAAREE
CCCCCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
QGKVEGRIRQLEDVLRRAEVGETPPNDGVVEPGMVVTYRFVGDAEDEVETFLLGAREIEP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEECC
EGLTVYSPQSPLGSAINGKAKGDTVSYLAPNGKELQVVIVDAVPYEG
CCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCC
>Mature Secondary Structure 
TQAAQPGTVWLTQDAYDKLRAELESLKGPVRQEIIARISSARDEGDLKENGAYHAAREE
CCCCCCCEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
QGKVEGRIRQLEDVLRRAEVGETPPNDGVVEPGMVVTYRFVGDAEDEVETFLLGAREIEP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEECC
EGLTVYSPQSPLGSAINGKAKGDTVSYLAPNGKELQVVIVDAVPYEG
CCEEEECCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12692562; 11572948 [H]